Restriction map for
pF1KE3971
(Circular) (Six-base) MAPSORT of: pF1KE3971.seq Check: 2610 from: 1 to: 4475
pF1KE3971 4475 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 654 1513 654
Size: 859 3616
Acc65I G'GTAC_C
Cuts at: 538 1491 538
Size: 953 3522
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 698 1220 2300 2732 3479 3747 635
Size: 63 522 1080 432 747 268 1363
Fragments arranged by size:
1363 1080 747 522 432 268 63
AflII C'TTAA_G
Cuts at: 958 958
Size: 4475
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 363 524 363
Size: 161 4314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 211
Size: 32 4443
AlwNI CAG_nnn'CTG
Cuts at: 1363 1780 3347 1363
Size: 417 1567 2491
Fragments arranged by size:
2491 1567 417
ApaI G_GGCC'C
Cuts at: 151 180 151
Size: 29 4446
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 1174 1479 3828 4084 1174
Size: 305 2349 256 1565
Fragments arranged by size:
2349 1565 305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 1495 1495
Size: 4475
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 522 555 522
Size: 33 4442
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 157 538 1491 1905 2153 2188 157
Size: 381 953 414 248 35 2444
Fragments arranged by size:
2444 953 414 381 248 35
BanII G_rGCy'C
Cuts at: 151 180 331 1489 2519 151
Size: 29 151 1158 1030 2107
Fragments arranged by size:
2107 1158 1030 151 29
BbeI G_GCGC'C
Cuts at: 161 1909 2157 161
Size: 1748 248 2479
Fragments arranged by size:
2479 1748 248
BciVI GTATCCnnnnn_n'
Cuts at: 201 2373 3134 4475 201
Size: 2172 761 1341 201
Fragments arranged by size:
2172 1341 761 201
BclI T'GATC_A
Cuts at: 468 900 1995 468
Size: 432 1095 2948
Fragments arranged by size:
2948 1095 432
BglI GCCn_nnn'nGGC
Cuts at: 112 155 112
Size: 43 4432
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 151 180 1393 2100 2193 3249 151
Size: 29 1213 707 93 1056 1377
Fragments arranged by size:
1377 1213 1056 707 93 29
BmrI ACTGGGnnnn_n'
Cuts at: 354 1687 1768 2103 4247 354
Size: 1333 81 335 2144 582
Fragments arranged by size:
2144 1333 582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 238 409 655 229
Size: 9 171 246 4049
Fragments arranged by size:
4049 246 171 9
Bpu10I CC'TnA_GC
Cuts at: 325 325
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1651 2530 3022 3320 589
Size: 1062 879 492 298 1744
Fragments arranged by size:
1744 1062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1376 4179 1376
Size: 2803 1672
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 251 1989 4026 6
Size: 245 1738 2037 455
Fragments arranged by size:
2037 1738 455 245
BsaHI Gr'CG_yC
Cuts at: 158 1906 2154 2856 4352 158
Size: 1748 248 702 1496 281
Fragments arranged by size:
1748 1496 702 281 248
BsaWI w'CCGG_w
Cuts at: 200 1664 2185 2863 3137 3284 3695 200
Size: 1464 521 678 274 147 411 980
Fragments arranged by size:
1464 980 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 382 412 211
Size: 30 141 30 4274
Fragments arranged by size:
4274 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 943 1178 943
Size: 235 4240
BseYI C'CCAG_C
Cuts at: 129 1900 2658 3235 3731 129
Size: 1771 758 577 496 873
Fragments arranged by size:
1771 873 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1057 1426 469
Size: 588 369 3518
Fragments arranged by size:
3518 588 369
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 331 1489 2267 2457 3249 331
Size: 1158 778 190 792 1557
Fragments arranged by size:
1557 1158 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 151 180 331 1393 1489 2100 2193 2267 2457
Size: 29 151 1062 96 611 93 74 190
Cuts at: 2457 2519 3249 151
Size: 62 730 1377
Fragments arranged by size:
1377 1062 730 611 190 151 96 93
74 62 29
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 780 969 3651 780
Size: 189 2682 1604
Fragments arranged by size:
2682 1604 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 783 1059 1525 1739 2041 2422 783
Size: 276 466 214 302 381 2836
Fragments arranged by size:
2836 466 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 2767 2821 4305 4470 50
Size: 2717 54 1484 165 55
Fragments arranged by size:
2717 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2863 2473
Size: 181 209 4085
Fragments arranged by size:
4085 209 181
BssSI C'ACGA_G
Cuts at: 2746 3104 2746
Size: 358 4117
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstEII G'GTnAC_C
Cuts at: 1288 1288
Size: 4475
BstXI CCAn_nnnn'nTGG
Cuts at: 711 931 711
Size: 220 4255
BstYI r'GATC_y
Cuts at: 1 252 405 1034 1500 1967 1990 2325 2571
Size: 251 153 629 466 467 23 335 246
Cuts at: 2571 3583 3669 3681 4027 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 629 467 466 449 346 335 251
246 153 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1114 1114
Size: 4475
BtgI C'CryG_G
Cuts at: 170 170
Size: 4475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1718 1391
Size: 327 4148
ClaI AT'CG_AT
Cuts at: 272 272
Size: 4475
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 2060 2234 2625 2652 3997 2060
Size: 174 391 27 1345 2538
Fragments arranged by size:
2538 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 2993 3139 4285 2683
Size: 310 146 1146 2873
Fragments arranged by size:
2873 1146 310 146
EcoICRI GAG'CTC
Cuts at: 329 1487 329
Size: 1158 3317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 238 409 635 655 698 1220 2300 2732
Size: 9 171 226 20 43 522 1080 432
Cuts at: 2732 3479 3747 229
Size: 747 268 957
Fragments arranged by size:
1080 957 747 522 432 268 226 171
43 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 958 958
Size: 4475
EcoO109I rG'GnC_Cy
Cuts at: 147 176 177 772 1614 147
Size: 29 1 595 842 3008
Fragments arranged by size:
3008 842 595 29 1
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 622 2494 622
Size: 1872 2603
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 161 1909 2157 3179 4158 161
Size: 1748 248 1022 979 478
Fragments arranged by size:
1748 1022 979 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 397 429 1233 1265 2317 2347 2349 2379 397
Size: 32 804 32 1052 30 2 30 2493
Fragments arranged by size:
2493 1052 804 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1101 1110 1514 1101
Size: 9 404 4062
Fragments arranged by size:
4062 404 9
HindIII A'AGCT_T
Cuts at: 236 787 236
Size: 551 3924
HpaI GTT'AAC
Cuts at: 1110 1110
Size: 4475
KasI G'GCGC_C
Cuts at: 157 1905 2153 157
Size: 1748 248 2479
Fragments arranged by size:
2479 1748 248
KpnI G_GTAC'C
Cuts at: 542 1495 542
Size: 953 3522
MfeI C'AATT_G
Cuts at: 748 748
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 762 3146 3330 762
Size: 2384 184 1907
Fragments arranged by size:
2384 1907 184
MscI TGG'CCA
Cuts at: 2236 2236
Size: 4475
MslI CAynn'nnrTG
Cuts at: 1376 2464 2591 1376
Size: 1088 127 3260
Fragments arranged by size:
3260 1088 127
MspA1I CmG'CkG
Cuts at: 483 498 1586 1900 2260 3273 3518 483
Size: 15 1088 314 360 1013 245 1440
Fragments arranged by size:
1440 1088 1013 360 314 245 15
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 158 1906 2154 158
Size: 1748 248 2479
Fragments arranged by size:
2479 1748 248
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NspI r_CATG'y
Cuts at: 1528 2028 2935 1528
Size: 500 907 3068
Fragments arranged by size:
3068 907 500
PasI CC'CwG_GG
Cuts at: 151 151
Size: 4475
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PflMI CCAn_nnn'nTGG
Cuts at: 387 387
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 243 3614 3646 211
Size: 32 3371 32 1040
Fragments arranged by size:
3371 1040 32 32
PpuMI rG'GwC_Cy
Cuts at: 772 772
Size: 4475
PspOMI G'GGCC_C
Cuts at: 147 176 147
Size: 29 4446
PstI C_TGCA'G
Cuts at: 503 853 1522 2207 503
Size: 350 669 685 2771
Fragments arranged by size:
2771 685 669 350
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 483 498 1900 2260 483
Size: 15 1402 360 2698
Fragments arranged by size:
2698 1402 360 15
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 331 1489 331
Size: 1158 3317
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 709 3992 709
Size: 3283 1192
SfcI C'TryA_G
Cuts at: 33 499 849 1518 2203 3196 3387 33
Size: 466 350 669 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 466 350 191
SfoI GGC'GCC
Cuts at: 159 1907 2155 159
Size: 1748 248 2479
Fragments arranged by size:
2479 1748 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 568 958 1630 2545 3037 3299 568
Size: 390 672 915 492 262 1744
Fragments arranged by size:
1744 915 672 492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StuI AGG'CCT
Cuts at: 977 977
Size: 4475
StyI C'CwwG_G
Cuts at: 924 978 1609 1963 924
Size: 54 631 354 3436
Fragments arranged by size:
3436 631 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2058 2191 2650 2859 2058
Size: 133 459 209 3674
Fragments arranged by size:
3674 459 209 133
TatI w'GTAC_w
Cuts at: 462 707 3990 462
Size: 245 3283 947
Fragments arranged by size:
3283 947 245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4443
Tth111I GACn'n_nGTC
Cuts at: 2272 2272
Size: 4475
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 226 647 226
Size: 421 4054
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AscI AvrII BbsI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I CspCI DraIII EcoRV FseI FspAI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI