Restriction map for
pF1KE4105
(Circular) (Six-base) MAPSORT of: pF1KE4105.seq Check: 6369 from: 1 to: 4538
pF1KE4105 4538 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 415 1466 1576 415
Size: 1051 110 3377
Fragments arranged by size:
3377 1051 110
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2363 2795 3542 3810 1192
Size: 1171 432 747 268 1920
Fragments arranged by size:
1920 1171 747 432 268
AflIII A'CryG_T
Cuts at: 1890 2994 3971 1890
Size: 1104 977 2457
Fragments arranged by size:
2457 1104 977
AgeI A'CCGG_T
Cuts at: 2926 2926
Size: 4538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 766 845 766
Size: 79 4459
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1366 1398 1366
Size: 32 4506
AlwNI CAG_nnn'CTG
Cuts at: 175 480 1843 3410 175
Size: 305 1363 1567 1303
Fragments arranged by size:
1567 1363 1303 305
ApaLI G'TGCA_C
Cuts at: 3308 3308
Size: 4538
ApoI r'AATT_y
Cuts at: 123 150 451 938 978 1122 1542 3891 4147
Size: 27 301 487 40 144 420 2349 256
Cuts at: 4147 123
Size: 514
Fragments arranged by size:
2349 514 487 420 301 256 144 40
27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 1558 1558
Size: 4538
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 122 155 575 608 122
Size: 33 420 33 4052
Fragments arranged by size:
4052 420 33 33
BamHI G'GATC_C
Cuts at: 1563 1563
Size: 4538
BanI G'GyrC_C
Cuts at: 1554 1968 2216 2251 1554
Size: 414 248 35 3841
Fragments arranged by size:
3841 414 248 35
BanII G_rGCy'C
Cuts at: 1490 1552 2582 1490
Size: 62 1030 3446
Fragments arranged by size:
3446 1030 62
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BbsI GAAGACnn'nnnn_
Cuts at: 897 1535 897
Size: 638 3900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4504
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3197 4538 2436
Size: 761 1341 2436
Fragments arranged by size:
2436 1341 761
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BfrBI ATG'CAT
Cuts at: 1010 1010
Size: 4538
BglII A'GATC_T
Cuts at: 1511 2053 4090 1511
Size: 542 2037 1959
Fragments arranged by size:
2037 1959 542
BlpI GC'TnA_GC
Cuts at: 220 1484 1650 220
Size: 1264 166 3108
Fragments arranged by size:
3108 1264 166
Bme1580I G_kGCm'C
Cuts at: 503 2163 2256 3312 503
Size: 1660 93 1056 1729
Fragments arranged by size:
1729 1660 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 506 1750 1831 2166 4310 506
Size: 1244 81 335 2144 734
Fragments arranged by size:
2144 1244 734 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 4538
Bpu10I CC'TnA_GC
Cuts at: 1196 1196
Size: 4538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1714 2593 3085 3383 1714
Size: 879 492 298 2869
Fragments arranged by size:
2869 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 2052 4089 6
Size: 2046 2037 455
Fragments arranged by size:
2046 2037 455
BsaHI Gr'CG_yC
Cuts at: 1969 2217 2919 4415 1969
Size: 248 702 1496 2092
Fragments arranged by size:
2092 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1162 1727 2248 2926 3200 3347 3758 1162
Size: 565 521 678 274 147 411 1942
Fragments arranged by size:
1942 678 565 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1283 1313 1283
Size: 30 4508
BseYI C'CCAG_C
Cuts at: 1963 2721 3298 3794 1963
Size: 758 577 496 2707
Fragments arranged by size:
2707 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 940
Size: 4538
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 534 797 1170 1490 1552 2330 2520 3312 534
Size: 263 373 320 62 778 190 792 1760
Fragments arranged by size:
1760 792 778 373 320 263 190 62
Bsp1286I G_dGCh'C
Cuts at: 503 534 797 1170 1490 1552 2163 2256 2330
Size: 31 263 373 320 62 611 93 74
Cuts at: 2330 2520 2582 3312 503
Size: 190 62 730 1729
Fragments arranged by size:
1729 730 611 373 320 263 190 93
74 62 62 31
BspEI T'CCGG_A
Cuts at: 3758 3758
Size: 4538
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 1802 2104 2485 1588
Size: 214 302 381 3641
Fragments arranged by size:
3641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1203 2830 2884 4368 4533 50
Size: 1153 1627 54 1484 165 55
Fragments arranged by size:
1627 1484 1153 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1012 2450 1012
Size: 1438 3100
BsrFI r'CCGG_y
Cuts at: 2536 2717 2926 2536
Size: 181 209 4148
Fragments arranged by size:
4148 209 181
BsrGI T'GTAC_A
Cuts at: 1290 1290
Size: 4538
BssSI C'ACGA_G
Cuts at: 2809 3167 2809
Size: 358 4180
BstAPI GCAn_nnn'nTGC
Cuts at: 1915 1915
Size: 4538
BstBI TT'CG_AA
Cuts at: 2899 2899
Size: 4538
BstYI r'GATC_y
Cuts at: 1 733 1511 1563 2030 2053 2388 2634 3646
Size: 732 778 52 467 23 335 246 1012
Cuts at: 3646 3732 3744 4090 1
Size: 86 12 346 449
Fragments arranged by size:
1012 778 732 467 449 346 335 246
86 52 23 12
BtgI C'CryG_G
Cuts at: 137 991 137
Size: 854 3684
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 1781 1781
Size: 4538
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DraIII CAC_nnn'GTG
Cuts at: 963 963
Size: 4538
DrdI GACnn_nn'nnGTC
Cuts at: 2244 3102 2244
Size: 858 3680
EaeI y'GGCC_r
Cuts at: 1358 2123 2297 2688 2715 4060 1358
Size: 765 174 391 27 1345 1836
Fragments arranged by size:
1836 1345 765 391 174 27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 1106 1210 2561 2771 1106
Size: 104 1351 210 2873
Fragments arranged by size:
2873 1351 210 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1295 2746 3056 3202 4348 1295
Size: 1451 310 146 1146 1485
Fragments arranged by size:
1485 1451 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1488 1550 1488
Size: 62 4476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2363 2795 3542 3810 1192
Size: 1171 432 747 268 1920
Fragments arranged by size:
1920 1171 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1677 1677
Size: 4538
EcoRI G'AATT_C
Cuts at: 150 1542 150
Size: 1392 3146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 591 2557 591
Size: 1966 2572
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 1972 2220 3242 4221 1972
Size: 248 1022 979 2289
Fragments arranged by size:
2289 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1085 1117 2380 2410 2412 2442 1085
Size: 32 1263 30 2 30 3181
Fragments arranged by size:
3181 1263 32 30 30 2
HincII GTy'rAC
Cuts at: 1104 1577 1104
Size: 473 4065
HindIII A'AGCT_T
Cuts at: 1392 1392
Size: 4538
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MfeI C'AATT_G
Cuts at: 668 668
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3393 3209
Size: 184 4354
MscI TGG'CCA
Cuts at: 1360 2299 1360
Size: 939 3599
MslI CAynn'nnrTG
Cuts at: 827 2527 2654 827
Size: 1700 127 2711
Fragments arranged by size:
2711 1700 127
MspA1I CmG'CkG
Cuts at: 1649 1963 2323 3336 3581 1649
Size: 314 360 1013 245 2606
Fragments arranged by size:
2606 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NsiI A_TGCA'T
Cuts at: 1012 1012
Size: 4538
NspI r_CATG'y
Cuts at: 1591 2091 2998 1591
Size: 500 907 3131
Fragments arranged by size:
3131 907 500
PciI A'CATG_T
Cuts at: 2994 2994
Size: 4538
PfoI T'CCnGG_A
Cuts at: 421 1184 421
Size: 763 3775
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PstI C_TGCA'G
Cuts at: 1585 2270 1585
Size: 685 3853
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 1963 2323 1963
Size: 360 4178
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 1490 1552 1490
Size: 62 4476
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 1210 2561 2771 1210
Size: 1351 210 2977
Fragments arranged by size:
2977 1351 210
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SfcI C'TryA_G
Cuts at: 33 613 1581 2266 3259 3450 33
Size: 580 968 685 993 191 1121
Fragments arranged by size:
1121 993 968 685 580 191
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 1693 2608 3100 3362 1693
Size: 915 492 262 2869
Fragments arranged by size:
2869 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1591 2091 1591
Size: 500 4038
StyI C'CwwG_G
Cuts at: 162 548 1672 2026 162
Size: 386 1124 354 2674
Fragments arranged by size:
2674 1124 386 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2121 2254 2713 2922 2121
Size: 133 459 209 3737
Fragments arranged by size:
3737 459 209 133
TatI w'GTAC_w
Cuts at: 1290 1502 4053 1290
Size: 212 2551 1775
Fragments arranged by size:
2551 1775 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 4055 4087 817
Size: 32 3206 32 1268
Fragments arranged by size:
3206 1268 32 32
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1035 1569 1035
Size: 534 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1392 2033 1392
Size: 641 3897
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
XmnI GAAnn'nnTTC
Cuts at: 894 894
Size: 4538
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BbvCI BglI BmgBI BmtI BpmI BseRI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI