Restriction map for
pF1KE4166
(Circular) (Six-base) MAPSORT of: pF1KE4166.seq Check: 9377 from: 1 to: 4967
pF1KE4166 4967 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 163 245 359 1226 163
Size: 82 114 867 3904
Fragments arranged by size:
3904 867 114 82
AccI GT'mk_AC
Cuts at: 1628 2005 1628
Size: 377 4590
Acc65I G'GTAC_C
Cuts at: 1983 1983
Size: 4967
AclI AA'CG_TT
Cuts at: 4807 4807
Size: 4967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 2792 3224 3971 4239 669
Size: 2123 432 747 268 1397
Fragments arranged by size:
2123 1397 747 432 268
AflIII A'CryG_T
Cuts at: 2319 3423 4400 2319
Size: 1104 977 2886
Fragments arranged by size:
2886 1104 977
AgeI A'CCGG_T
Cuts at: 3355 3355
Size: 4967
AhdI GACnn_n'nnGTC
Cuts at: 474 1602 474
Size: 1128 3839
AlwNI CAG_nnn'CTG
Cuts at: 672 705 783 2272 3839 672
Size: 33 78 1489 1567 1800
Fragments arranged by size:
1800 1567 1489 78 33
ApaI G_GGCC'C
Cuts at: 434 1135 1380 434
Size: 701 245 4021
Fragments arranged by size:
4021 701 245
ApaLI G'TGCA_C
Cuts at: 3737 3737
Size: 4967
ApoI r'AATT_y
Cuts at: 1971 4320 4576 1971
Size: 2349 256 2362
Fragments arranged by size:
2362 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4967
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
AvaI C'yCGr_G
Cuts at: 196 523 1135 1987 196
Size: 327 612 852 3176
Fragments arranged by size:
3176 852 612 327
AvrII C'CTAG_G
Cuts at: 1245 1245
Size: 4967
BamHI G'GATC_C
Cuts at: 398 1992 398
Size: 1594 3373
BanI G'GyrC_C
Cuts at: 258 438 761 1072 1433 1983 2397 2645 2680
Size: 180 323 311 361 550 414 248 35
Cuts at: 2680 258
Size: 2545
Fragments arranged by size:
2545 550 414 361 323 311 248 180
35
BanII G_rGCy'C
Cuts at: 434 487 734 1010 1135 1142 1380 1793 1981
Size: 53 247 276 125 7 238 413 188
Cuts at: 1981 3011 434
Size: 1030 2390
Fragments arranged by size:
2390 1030 413 276 247 238 188 125
53 7
BbeI G_GCGC'C
Cuts at: 1076 2401 2649 1076
Size: 1325 248 3394
Fragments arranged by size:
3394 1325 248
BbsI GAAGACnn'nnnn_
Cuts at: 163 802 163
Size: 639 4328
BbvCI CC'TCA_GC
Cuts at: 1924 1924
Size: 4967
BciVI GTATCCnnnnn_n'
Cuts at: 1180 2865 3626 4967 1180
Size: 1685 761 1341 1180
Fragments arranged by size:
1685 1341 1180 761
BclI T'GATC_A
Cuts at: 2487 2487
Size: 4967
BglI GCCn_nnn'nGGC
Cuts at: 598 778 1825 1912 598
Size: 180 1047 87 3653
Fragments arranged by size:
3653 1047 180 87
BglII A'GATC_T
Cuts at: 2482 4519 2482
Size: 2037 2930
BlpI GC'TnA_GC
Cuts at: 2079 2079
Size: 4967
Bme1580I G_kGCm'C
Cuts at: 263 434 443 764 989 1135 1380 1436 1528
Size: 171 9 321 225 146 245 56 92
Cuts at: 1528 2592 2685 3741 263
Size: 1064 93 1056 1489
Fragments arranged by size:
1489 1064 1056 321 245 225 171 146
93 92 56 9
BmgBI CAC'GTC
Cuts at: 120 792 120
Size: 672 4295
BmrI ACTGGGnnnn_n'
Cuts at: 145 2179 2260 2595 4739 145
Size: 2034 81 335 2144 373
Fragments arranged by size:
2144 2034 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 4967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 967 805
Size: 162 4805
Bpu10I CC'TnA_GC
Cuts at: 1924 1924
Size: 4967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2143 3022 3514 3812 2143
Size: 879 492 298 3298
Fragments arranged by size:
3298 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 526 895 4671 526
Size: 369 3776 822
Fragments arranged by size:
3776 822 369
BsaAI yAC'GTr
Cuts at: 984 2320 984
Size: 1336 3631
BsaBI GATnn'nnATC
Cuts at: 6 229 2481 4518 6
Size: 223 2252 2037 455
Fragments arranged by size:
2252 2037 455 223
BsaHI Gr'CG_yC
Cuts at: 160 242 356 1073 1223 1556 2398 2646 3348
Size: 82 114 717 150 333 842 248 702
Cuts at: 3348 4844 160
Size: 1496 283
Fragments arranged by size:
1496 842 717 702 333 283 248 150
114 82
BsaWI w'CCGG_w
Cuts at: 1226 2156 2677 3355 3629 3776 4187 1226
Size: 930 521 678 274 147 411 2006
Fragments arranged by size:
2006 930 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 952 982 952
Size: 30 4937
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 214 235 319 331 379 1665 202
Size: 12 21 84 12 48 1286 3504
Fragments arranged by size:
3504 1286 84 48 21 12 12
BseYI C'CCAG_C
Cuts at: 1116 1782 1792 1811 1890 2392 3150 3727 4223
Size: 666 10 19 79 502 758 577 496
Cuts at: 4223 1116
Size: 1860
Fragments arranged by size:
1860 758 666 577 502 496 79 19
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 1054 1165 1501 828
Size: 226 111 336 4294
Fragments arranged by size:
4294 336 226 111
BsiEI CG_ry'CG
Cuts at: 104 478 894 1170 1422 1727 2509 2555 3763
Size: 374 416 276 252 305 782 46 1208
Cuts at: 3763 104
Size: 1308
Fragments arranged by size:
1308 1208 782 416 374 305 276 252
46
BsiHKAI G_wGCw'C
Cuts at: 487 503 734 1010 1142 1981 2759 2949 3741
Size: 16 231 276 132 839 778 190 792
Cuts at: 3741 487
Size: 1713
Fragments arranged by size:
1713 839 792 778 276 231 190 132
16
BsmBI CGTCTCn'nnnn_
Cuts at: 363 363
Size: 4967
Bsp1286I G_dGCh'C
Cuts at: 263 434 443 487 503 734 764 989 1010
Size: 171 9 44 16 231 30 225 21
Cuts at: 1010 1135 1142 1380 1436 1528 1793 1981 2592
Size: 125 7 238 56 92 265 188 611
Cuts at: 2592 2685 2759 2949 3011 3741 263
Size: 93 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 265 238 231 225 190
188 171 125 93 92 74 62 56
44 30 21 16 9 7
BspEI T'CCGG_A
Cuts at: 1226 4187 1226
Size: 2961 2006
BspHI T'CATG_A
Cuts at: 509 4143 509
Size: 3634 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 479 544 1473 1697 2017 2231 2533 2914 479
Size: 65 929 224 320 214 302 381 2532
Fragments arranged by size:
2532 929 381 320 302 224 214 65
BsrBI CCG'CTC
Cuts at: 50 1756 3259 3313 4797 4962 50
Size: 1706 1503 54 1484 165 55
Fragments arranged by size:
1706 1503 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2879 2879
Size: 4967
BsrFI r'CCGG_y
Cuts at: 839 893 1165 1169 1722 1745 2965 3146 3355
Size: 54 272 4 553 23 1220 181 209
Cuts at: 3355 839
Size: 2451
Fragments arranged by size:
2451 1220 553 272 209 181 54 23
4
BssSI C'ACGA_G
Cuts at: 898 1307 1443 3238 3596 898
Size: 409 136 1795 358 2269
Fragments arranged by size:
2269 1795 409 358 136
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 2344 1618
Size: 726 4241
BstBI TT'CG_AA
Cuts at: 387 3328 387
Size: 2941 2026
BstEII G'GTnAC_C
Cuts at: 1209 1209
Size: 4967
BstXI CCAn_nnnn'nTGG
Cuts at: 1551 1551
Size: 4967
BstYI r'GATC_y
Cuts at: 1 320 398 1992 2459 2482 2817 3063 4075
Size: 319 78 1594 467 23 335 246 1012
Cuts at: 4075 4161 4173 4519 1
Size: 86 12 346 449
Fragments arranged by size:
1594 1012 467 449 346 335 319 246
86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 562 635 562
Size: 73 4894
BtgI C'CryG_G
Cuts at: 236 488 1085 1380 1422 1823 1910 236
Size: 252 597 295 42 401 87 3293
Fragments arranged by size:
3293 597 401 295 252 87 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 953 1532 2917 3098 3261 953
Size: 579 1385 181 163 2659
Fragments arranged by size:
2659 1385 579 181 163
BtsI GCAGTG_nn'
Cuts at: 1599 2210 1599
Size: 611 4356
DraI TTT'AAA
Cuts at: 1966 1966
Size: 4967
DraIII CAC_nnn'GTG
Cuts at: 273 1618 273
Size: 1345 3622
DrdI GACnn_nn'nnGTC
Cuts at: 2673 3531 2673
Size: 858 4109
EaeI y'GGCC_r
Cuts at: 551 891 1167 1419 1541 1724 1826 1913 2552
Size: 340 276 252 122 183 102 87 639
Cuts at: 2552 2726 3117 3144 4489 551
Size: 174 391 27 1345 1029
Fragments arranged by size:
1345 1029 639 391 340 276 252 183
174 122 102 87 27
EagI C'GGCC_G
Cuts at: 891 1167 1419 1724 2552 891
Size: 276 252 305 828 3306
Fragments arranged by size:
3306 828 305 276 252
EarI CTCTTCn'nnn_
Cuts at: 722 868 883 1309 1872 1948 2990 3200 722
Size: 146 15 426 563 76 1042 210 2489
Fragments arranged by size:
2489 1042 563 426 210 146 76 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3175 3485 3631 4777 3175
Size: 310 146 1146 3365
Fragments arranged by size:
3365 1146 310 146
EcoICRI GAG'CTC
Cuts at: 485 732 1008 1140 1979 485
Size: 247 276 132 839 3473
Fragments arranged by size:
3473 839 276 247 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 805 967 2792 3224 3971 4239 669
Size: 136 162 1825 432 747 268 1397
Fragments arranged by size:
1825 1397 747 432 268 162 136
EcoNI CCTnn'n_nnAGG
Cuts at: 709 1363 1516 709
Size: 654 153 4160
Fragments arranged by size:
4160 654 153
EcoO109I rG'GnC_Cy
Cuts at: 204 430 702 780 1131 1132 1376 1944 2106
Size: 226 272 78 351 1 244 568 162
Cuts at: 2106 204
Size: 3065
Fragments arranged by size:
3065 568 351 272 244 226 162 78
1
EcoRI G'AATT_C
Cuts at: 1971 1971
Size: 4967
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1416 2986 1416
Size: 1570 3397
FseI GG_CCGG'CC
Cuts at: 1169 1169
Size: 4967
FspI TGC'GCA
Cuts at: 124 2748 124
Size: 2624 2343
FspAI rTGC'GCAy
Cuts at: 124 124
Size: 4967
HaeII r_GCGC'y
Cuts at: 673 718 927 1076 1126 2401 2649 3671 4650
Size: 45 209 149 50 1275 248 1022 979
Cuts at: 4650 673
Size: 990
Fragments arranged by size:
1275 1022 990 979 248 209 149 50
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 952 984 1588 1620 2809 2839 2841
Size: 32 460 32 604 32 1189 30 2
Cuts at: 2841 2871 460
Size: 30 2556
Fragments arranged by size:
2556 1189 604 460 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2006 2006
Size: 4967
KasI G'GCGC_C
Cuts at: 1072 2397 2645 1072
Size: 1325 248 3394
Fragments arranged by size:
3394 1325 248
KpnI G_GTAC'C
Cuts at: 1987 1987
Size: 4967
MluI A'CGCG_T
Cuts at: 4400 4400
Size: 4967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3638 3822 3638
Size: 184 4783
MscI TGG'CCA
Cuts at: 1543 2728 1543
Size: 1185 3782
MslI CAynn'nnrTG
Cuts at: 451 2956 3083 451
Size: 2505 127 2335
Fragments arranged by size:
2505 2335 127
MspA1I CmG'CkG
Cuts at: 238 1347 1424 2078 2392 2752 3765 4010 238
Size: 1109 77 654 314 360 1013 245 1195
Fragments arranged by size:
1195 1109 1013 654 360 314 245 77
NaeI GCC'GGC
Cuts at: 895 1167 3148 895
Size: 272 1981 2714
Fragments arranged by size:
2714 1981 272
NarI GG'CG_CC
Cuts at: 1073 2398 2646 1073
Size: 1325 248 3394
Fragments arranged by size:
3394 1325 248
NcoI C'CATG_G
Cuts at: 488 1823 488
Size: 1335 3632
NgoMIV G'CCGG_C
Cuts at: 893 1165 3146 893
Size: 272 1981 2714
Fragments arranged by size:
2714 1981 272
NspI r_CATG'y
Cuts at: 2020 2520 3427 2020
Size: 500 907 3560
Fragments arranged by size:
3560 907 500
PasI CC'CwG_GG
Cuts at: 434 1127 434
Size: 693 4274
PciI A'CATG_T
Cuts at: 3423 3423
Size: 4967
PflMI CCAn_nnn'nTGG
Cuts at: 273 1644 273
Size: 1371 3596
PmeI GTTT'AAAC
Cuts at: 1966 1966
Size: 4967
PmlI CAC'GTG
Cuts at: 984 984
Size: 4967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 4106
Size: 32 4935
PpuMI rG'GwC_Cy
Cuts at: 204 702 1944 204
Size: 498 1242 3227
Fragments arranged by size:
3227 1242 498
PspOMI G'GGCC_C
Cuts at: 430 1131 1376 430
Size: 701 245 4021
Fragments arranged by size:
4021 701 245
PstI C_TGCA'G
Cuts at: 1000 1409 2014 2699 1000
Size: 409 605 685 3268
Fragments arranged by size:
3268 685 605 409
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4967
PvuII CAG'CTG
Cuts at: 2392 2752 2392
Size: 360 4607
RsrII CG'GwC_CG
Cuts at: 3162 3162
Size: 4967
SacI G_AGCT'C
Cuts at: 487 734 1010 1142 1981 487
Size: 247 276 132 839 3473
Fragments arranged by size:
3473 839 276 247 132
SacII CC_GC'GG
Cuts at: 239 1425 239
Size: 1186 3781
SalI G'TCGA_C
Cuts at: 2004 2004
Size: 4967
SapI GCTCTTCn'nnn_
Cuts at: 722 883 1948 2990 3200 722
Size: 161 1065 1042 210 2489
Fragments arranged by size:
2489 1065 1042 210 161
SbfI CC_TGCA'GG
Cuts at: 1409 2014 1409
Size: 605 4362
ScaI AGT'ACT
Cuts at: 1504 4484 1504
Size: 2980 1987
SfcI C'TryA_G
Cuts at: 33 933 996 1405 1938 2010 2695 3688 3879
Size: 900 63 409 533 72 685 993 191
Cuts at: 3879 33
Size: 1121
Fragments arranged by size:
1121 993 900 685 533 409 191 72
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 778 1912 778
Size: 1134 3833
SfoI GGC'GCC
Cuts at: 1074 2399 2647 1074
Size: 1325 248 3394
Fragments arranged by size:
3394 1325 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
SmaI CCC'GGG
Cuts at: 1989 1989
Size: 4967
SmlI C'TyrA_G
Cuts at: 2122 3037 3529 3791 2122
Size: 915 492 262 3298
Fragments arranged by size:
3298 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4967
SphI G_CATG'C
Cuts at: 2020 2520 2020
Size: 500 4467
StuI AGG'CCT
Cuts at: 1411 1411
Size: 4967
StyI C'CwwG_G
Cuts at: 488 1017 1236 1245 1679 1823 2101 2455 488
Size: 529 219 9 434 144 278 354 3000
Fragments arranged by size:
3000 529 434 354 278 219 144 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 280 1825 2550 2683 3142 3351 280
Size: 1545 725 133 459 209 1896
Fragments arranged by size:
1896 1545 725 459 209 133
TatI w'GTAC_w
Cuts at: 1502 4482 1502
Size: 2980 1987
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1136 1168 1829 1861 4484 4516 1136
Size: 32 661 32 2623 32 1587
Fragments arranged by size:
2623 1587 661 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2764 2764
Size: 4967
XbaI T'CTAG_A
Cuts at: 1998 1998
Size: 4967
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 450 1124 2462 270
Size: 180 674 1338 2775
Fragments arranged by size:
2775 1338 674 180
XmaI C'CCGG_G
Cuts at: 1987 1987
Size: 4967
ZraI GAC'GTC
Cuts at: 161 243 357 1224 161
Size: 82 114 867 3904
Fragments arranged by size:
3904 867 114 82
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI BaeI
BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I
ClaI CspCI EcoRV HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI