Restriction map for
pF1KE4224
(Circular) (Six-base) MAPSORT of: pF1KE4224.seq Check: 5545 from: 1 to: 5471
pF1KE4224 5471 bp
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1873 1873
Size: 5471
AccI GT'mk_AC
Cuts at: 1946 2509 1946
Size: 563 4908
Acc65I G'GTAC_C
Cuts at: 2487 2487
Size: 5471
AclI AA'CG_TT
Cuts at: 5311 5311
Size: 5471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3296 3728 4475 4743 3296
Size: 432 747 268 4024
Fragments arranged by size:
4024 747 432 268
AflIII A'CryG_T
Cuts at: 2823 3927 4904 2823
Size: 1104 977 3390
Fragments arranged by size:
3390 1104 977
AgeI A'CCGG_T
Cuts at: 3859 3859
Size: 5471
AhdI GACnn_n'nnGTC
Cuts at: 260 260
Size: 5471
AlwNI CAG_nnn'CTG
Cuts at: 123 137 1689 2776 4343 123
Size: 14 1552 1087 1567 1251
Fragments arranged by size:
1567 1552 1251 1087 14
ApaLI G'TGCA_C
Cuts at: 4241 4241
Size: 5471
ApoI r'AATT_y
Cuts at: 373 381 460 555 1443 2475 4824 5080 373
Size: 8 79 95 888 1032 2349 256 764
Fragments arranged by size:
2349 1032 888 764 256 95 79 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 5471
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5471
AvaI C'yCGr_G
Cuts at: 193 643 2227 2306 2491 193
Size: 450 1584 79 185 3173
Fragments arranged by size:
3173 1584 450 185 79
BamHI G'GATC_C
Cuts at: 170 2496 170
Size: 2326 3145
BanI G'GyrC_C
Cuts at: 2487 2901 3149 3184 2487
Size: 414 248 35 4774
Fragments arranged by size:
4774 414 248 35
BanII G_rGCy'C
Cuts at: 224 854 1862 2485 3515 224
Size: 630 1008 623 1030 2180
Fragments arranged by size:
2180 1030 1008 630 623
BbeI G_GCGC'C
Cuts at: 2905 3153 2905
Size: 248 5223
BbsI GAAGACnn'nnnn_
Cuts at: 1471 1495 1471
Size: 24 5447
BciVI GTATCCnnnnn_n'
Cuts at: 2327 3369 4130 5471 2327
Size: 1042 761 1341 2327
Fragments arranged by size:
2327 1341 1042 761
BclI T'GATC_A
Cuts at: 2991 2991
Size: 5471
BfrBI ATG'CAT
Cuts at: 353 1313 353
Size: 960 4511
BglI GCCn_nnn'nGGC
Cuts at: 1899 1899
Size: 5471
BglII A'GATC_T
Cuts at: 1030 2986 5023 1030
Size: 1956 2037 1478
Fragments arranged by size:
2037 1956 1478
BlpI GC'TnA_GC
Cuts at: 2583 2583
Size: 5471
Bme1580I G_kGCm'C
Cuts at: 3096 3189 4245 3096
Size: 93 1056 4322
Fragments arranged by size:
4322 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2130 2683 2764 3099 5243 2130
Size: 553 81 335 2144 2358
Fragments arranged by size:
2358 2144 553 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3838 3838
Size: 5471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 418 1055 1459 1981 2373 145
Size: 273 637 404 522 392 3243
Fragments arranged by size:
3243 637 522 404 392 273
Bpu10I CC'TnA_GC
Cuts at: 1856 1856
Size: 5471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2647 3526 4018 4316 2647
Size: 879 492 298 3802
Fragments arranged by size:
3802 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5175 5175
Size: 5471
BsaAI yAC'GTr
Cuts at: 370 2824 370
Size: 2454 3017
BsaBI GATnn'nnATC
Cuts at: 6 2985 5022 6
Size: 2979 2037 455
Fragments arranged by size:
2979 2037 455
BsaHI Gr'CG_yC
Cuts at: 1834 2902 3150 3852 5348 1834
Size: 1068 248 702 1496 1957
Fragments arranged by size:
1957 1496 1068 702 248
BsaWI w'CCGG_w
Cuts at: 2660 3181 3859 4133 4280 4691 2660
Size: 521 678 274 147 411 3440
Fragments arranged by size:
3440 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1512 2243 1512
Size: 731 4740
BseYI C'CCAG_C
Cuts at: 275 2896 3654 4231 4727 275
Size: 2621 758 577 496 1019
Fragments arranged by size:
2621 1019 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2152 3013 3059 4267 104
Size: 2048 861 46 1208 1308
Fragments arranged by size:
2048 1308 1208 861 46
BsiHKAI G_wGCw'C
Cuts at: 224 854 1028 2485 3263 3453 4245 224
Size: 630 174 1457 778 190 792 1450
Fragments arranged by size:
1457 1450 792 778 630 190 174
BsmBI CGTCTCn'nnnn_
Cuts at: 2045 2045
Size: 5471
Bsp1286I G_dGCh'C
Cuts at: 224 854 1028 1862 2485 3096 3189 3263 3453
Size: 630 174 834 623 611 93 74 190
Cuts at: 3453 3515 4245 224
Size: 62 730 1450
Fragments arranged by size:
1450 834 730 630 623 611 190 174
93 74 62
BspEI T'CCGG_A
Cuts at: 4691 4691
Size: 5471
BspHI T'CATG_A
Cuts at: 854 1517 4647 854
Size: 663 3130 1678
Fragments arranged by size:
3130 1678 663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1873 2521 2735 3037 3418 1873
Size: 648 214 302 381 3926
Fragments arranged by size:
3926 648 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3763 3817 5301 5466 50
Size: 3713 54 1484 165 55
Fragments arranged by size:
3713 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1431 3383 1431
Size: 1952 3519
BsrFI r'CCGG_y
Cuts at: 617 3469 3650 3859 617
Size: 2852 181 209 2229
Fragments arranged by size:
2852 2229 209 181
BsrGI T'GTAC_A
Cuts at: 1355 2379 1355
Size: 1024 4447
BssSI C'ACGA_G
Cuts at: 1331 1577 3742 4100 1331
Size: 246 2165 358 2702
Fragments arranged by size:
2702 2165 358 246
BstAPI GCAn_nnn'nTGC
Cuts at: 2848 2848
Size: 5471
BstBI TT'CG_AA
Cuts at: 3832 3832
Size: 5471
BstXI CCAn_nnnn'nTGG
Cuts at: 2019 2019
Size: 5471
BstYI r'GATC_y
Cuts at: 1 170 1030 2496 2963 2986 3321 3567 4579
Size: 169 860 1466 467 23 335 246 1012
Cuts at: 4579 4665 4677 5023 1
Size: 86 12 346 449
Fragments arranged by size:
1466 1012 860 467 449 346 335 246
169 86 23 12
BtgI C'CryG_G
Cuts at: 107 154 1937 107
Size: 47 1783 3641
Fragments arranged by size:
3641 1783 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 231 3421 3602 3765 231
Size: 3190 181 163 1937
Fragments arranged by size:
3190 1937 181 163
BtsI GCAGTG_nn'
Cuts at: 1198 2714 1198
Size: 1516 3955
ClaI AT'CG_AT
Cuts at: 819 819
Size: 5471
DraI TTT'AAA
Cuts at: 2470 2470
Size: 5471
DrdI GACnn_nn'nnGTC
Cuts at: 2151 3177 4035 2151
Size: 1026 858 3587
Fragments arranged by size:
3587 1026 858
EaeI y'GGCC_r
Cuts at: 1940 3056 3230 3621 3648 4993 1940
Size: 1116 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 1116 391 174 27
EagI C'GGCC_G
Cuts at: 3056 3056
Size: 5471
EarI CTCTTCn'nnn_
Cuts at: 842 1172 1903 3494 3704 842
Size: 330 731 1591 210 2609
Fragments arranged by size:
2609 1591 731 330 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3679 3989 4135 5281 3679
Size: 310 146 1146 3869
Fragments arranged by size:
3869 1146 310 146
EcoICRI GAG'CTC
Cuts at: 222 852 2483 222
Size: 630 1631 3210
Fragments arranged by size:
3210 1631 630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 418 1055 1459 1981 2373 3296 3728 4475
Size: 273 637 404 522 392 923 432 747
Cuts at: 4475 4743 145
Size: 268 873
Fragments arranged by size:
923 873 747 637 522 432 404 392
273 268
EcoO109I rG'GnC_Cy
Cuts at: 2610 2610
Size: 5471
EcoRI G'AATT_C
Cuts at: 1443 2475 1443
Size: 1032 4439
EcoRV GAT'ATC
Cuts at: 600 600
Size: 5471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 493 3490 493
Size: 2997 2474
FspI TGC'GCA
Cuts at: 3252 3252
Size: 5471
HaeII r_GCGC'y
Cuts at: 2905 3153 4175 5154 2905
Size: 248 1022 979 3222
Fragments arranged by size:
3222 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 246 278 1015 1047 2110 2142 3313
Size: 32 52 32 737 32 1063 32 1171
Cuts at: 3313 3343 3345 3375 162
Size: 30 2 30 2258
Fragments arranged by size:
2258 1171 1063 737 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2510 2510
Size: 5471
KasI G'GCGC_C
Cuts at: 2901 3149 2901
Size: 248 5223
KpnI G_GTAC'C
Cuts at: 2491 2491
Size: 5471
MluI A'CGCG_T
Cuts at: 4904 4904
Size: 5471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1259 1616 2209 2225 2278 4142 4326 1178
Size: 81 357 593 16 53 1864 184 2323
Fragments arranged by size:
2323 1864 593 357 184 81 53 16
MscI TGG'CCA
Cuts at: 3232 3232
Size: 5471
MslI CAynn'nnrTG
Cuts at: 629 3460 3587 629
Size: 2831 127 2513
Fragments arranged by size:
2831 2513 127
MspA1I CmG'CkG
Cuts at: 279 2056 2209 2582 2896 3256 4269 4514 279
Size: 1777 153 373 314 360 1013 245 1236
Fragments arranged by size:
1777 1236 1013 373 360 314 245 153
NaeI GCC'GGC
Cuts at: 3652 3652
Size: 5471
NarI GG'CG_CC
Cuts at: 2902 3150 2902
Size: 248 5223
NcoI C'CATG_G
Cuts at: 107 107
Size: 5471
NgoMIV G'CCGG_C
Cuts at: 3650 3650
Size: 5471
NsiI A_TGCA'T
Cuts at: 355 1315 355
Size: 960 4511
NspI r_CATG'y
Cuts at: 984 1424 2524 3024 3931 984
Size: 440 1100 500 907 2524
Fragments arranged by size:
2524 1100 907 500 440
PasI CC'CwG_GG
Cuts at: 2062 2062
Size: 5471
PciI A'CATG_T
Cuts at: 3927 3927
Size: 5471
PflMI CCAn_nnn'nTGG
Cuts at: 1599 2094 1599
Size: 495 4976
PfoI T'CCnGG_A
Cuts at: 161 929 2070 161
Size: 768 1141 3562
Fragments arranged by size:
3562 1141 768
PmeI GTTT'AAAC
Cuts at: 2470 2470
Size: 5471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 626 658 4610 4642 626
Size: 32 3952 32 1455
Fragments arranged by size:
3952 1455 32 32
PspXI vC'TCGA_Gb
Cuts at: 2306 2306
Size: 5471
PstI C_TGCA'G
Cuts at: 119 665 1452 2518 3203 119
Size: 546 787 1066 685 2387
Fragments arranged by size:
2387 1066 787 685 546
PvuI CG_AT'CG
Cuts at: 104 2152 104
Size: 2048 3423
PvuII CAG'CTG
Cuts at: 2056 2209 2896 3256 2056
Size: 153 687 360 4271
Fragments arranged by size:
4271 687 360 153
RsrII CG'GwC_CG
Cuts at: 3666 3666
Size: 5471
SacI G_AGCT'C
Cuts at: 224 854 2485 224
Size: 630 1631 3210
Fragments arranged by size:
3210 1631 630
SalI G'TCGA_C
Cuts at: 2508 2508
Size: 5471
SapI GCTCTTCn'nnn_
Cuts at: 842 3494 3704 842
Size: 2652 210 2609
Fragments arranged by size:
2652 2609 210
SbfI CC_TGCA'GG
Cuts at: 2518 2518
Size: 5471
ScaI AGT'ACT
Cuts at: 4988 4988
Size: 5471
SfcI C'TryA_G
Cuts at: 33 115 661 790 1448 2051 2264 2514 3199
Size: 82 546 129 658 603 213 250 685
Cuts at: 3199 4192 4383 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 658 603 546 250 213
191 129 82
SfoI GGC'GCC
Cuts at: 2903 3151 2903
Size: 248 5223
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5471
SmaI CCC'GGG
Cuts at: 2229 2493 2229
Size: 264 5207
SmlI C'TyrA_G
Cuts at: 193 643 2306 2626 3541 4033 4295 193
Size: 450 1663 320 915 492 262 1369
Fragments arranged by size:
1663 1369 915 492 450 320 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5471
SphI G_CATG'C
Cuts at: 2524 3024 2524
Size: 500 4971
SspI AAT'ATT
Cuts at: 1788 1788
Size: 5471
StuI AGG'CCT
Cuts at: 942 942
Size: 5471
StyI C'CwwG_G
Cuts at: 107 452 878 1347 1897 1994 2605 2959 107
Size: 345 426 469 550 97 611 354 2619
Fragments arranged by size:
2619 611 550 469 426 354 345 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1198 3054 3187 3646 3855 1198
Size: 1856 133 459 209 2814
Fragments arranged by size:
2814 1856 459 209 133
TatI w'GTAC_w
Cuts at: 1355 2379 4986 1355
Size: 1024 2607 1840
Fragments arranged by size:
2607 1840 1024
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4988 5020 4988
Size: 32 5439
Tth111I GACn'n_nGTC
Cuts at: 3268 3268
Size: 5471
XbaI T'CTAG_A
Cuts at: 2502 2502
Size: 5471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1935 2966 1935
Size: 1031 4440
XhoI C'TCGA_G
Cuts at: 193 643 2306 193
Size: 450 1663 3358
Fragments arranged by size:
3358 1663 450
XmaI C'CCGG_G
Cuts at: 2227 2491 2227
Size: 264 5207
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BssHII BstEII BstZ17I Bsu36I CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XmnI ZraI