Restriction map for
pF1KE4235
(Circular) (Six-base) MAPSORT of: pF1KE4235.seq Check: 6093 from: 1 to: 5687
pF1KE4235 5687 bp
With 182 enzymes: SgfI *
October 29, 2018 20:57 ..
AatII G_ACGT'C
Cuts at: 2630 2630
Size: 5687
AccI GT'mk_AC
Cuts at: 2725 2725
Size: 5687
Acc65I G'GTAC_C
Cuts at: 2703 2703
Size: 5687
AclI AA'CG_TT
Cuts at: 1682 5527 1682
Size: 3845 1842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 3512 3944 4691 4959 435
Size: 3077 432 747 268 1163
Fragments arranged by size:
3077 1163 747 432 268
AflII C'TTAA_G
Cuts at: 1030 2532 1030
Size: 1502 4185
AflIII A'CryG_T
Cuts at: 602 1700 3039 4143 5120 602
Size: 1098 1339 1104 977 1169
Fragments arranged by size:
1339 1169 1104 1098 977
AgeI A'CCGG_T
Cuts at: 4075 4075
Size: 5687
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 2602
Size: 32 5655
AlwNI CAG_nnn'CTG
Cuts at: 1065 1677 2992 4559 1065
Size: 612 1315 1567 2193
Fragments arranged by size:
2193 1567 1315 612
ApaI G_GGCC'C
Cuts at: 889 889
Size: 5687
ApaLI G'TGCA_C
Cuts at: 4457 4457
Size: 5687
ApoI r'AATT_y
Cuts at: 1016 2691 5040 5296 1016
Size: 1675 2349 256 1407
Fragments arranged by size:
2349 1675 1407 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
AvaI C'yCGr_G
Cuts at: 160 316 372 2707 160
Size: 156 56 2335 3140
Fragments arranged by size:
3140 2335 156 56
BamHI G'GATC_C
Cuts at: 1636 2712 1636
Size: 1076 4611
BanI G'GyrC_C
Cuts at: 376 1999 2703 3117 3365 3400 376
Size: 1623 704 414 248 35 2663
Fragments arranged by size:
2663 1623 704 414 248 35
BanII G_rGCy'C
Cuts at: 167 464 551 889 1995 2077 2269 2655 2701
Size: 297 87 338 1106 82 192 386 46
Cuts at: 2701 3731 167
Size: 1030 2123
Fragments arranged by size:
2123 1106 1030 386 338 297 192 87
82 46
BbeI G_GCGC'C
Cuts at: 380 3121 3369 380
Size: 2741 248 2698
Fragments arranged by size:
2741 2698 248
BbsI GAAGACnn'nnnn_
Cuts at: 1146 1652 1703 1146
Size: 506 51 5130
Fragments arranged by size:
5130 506 51
BbvCI CC'TCA_GC
Cuts at: 844 1670 844
Size: 826 4861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5653
BciVI GTATCCnnnnn_n'
Cuts at: 1455 1810 3585 4346 5687 1455
Size: 355 1775 761 1341 1455
Fragments arranged by size:
1775 1455 1341 761 355
BclI T'GATC_A
Cuts at: 3207 3207
Size: 5687
BglI GCCn_nnn'nGGC
Cuts at: 112 374 112
Size: 262 5425
BglII A'GATC_T
Cuts at: 485 998 3202 5239 485
Size: 513 2204 2037 933
Fragments arranged by size:
2204 2037 933 513
BlpI GC'TnA_GC
Cuts at: 445 2263 2799 445
Size: 1818 536 3333
Fragments arranged by size:
3333 1818 536
Bme1580I G_kGCm'C
Cuts at: 889 3312 3405 4461 889
Size: 2423 93 1056 2115
Fragments arranged by size:
2423 2115 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 419 480 1964 2144 2576 2899 2980 3315 5459
Size: 61 1484 180 432 323 81 335 2144
Cuts at: 5459 419
Size: 647
Fragments arranged by size:
2144 1484 647 432 335 323 180 81
61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4054 4054
Size: 5687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 664 1042 1128 1435 2251 2406 514
Size: 150 378 86 307 816 155 3795
Fragments arranged by size:
3795 816 378 307 155 150 86
Bpu10I CC'TnA_GC
Cuts at: 743 844 1670 743
Size: 101 826 4760
Fragments arranged by size:
4760 826 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1590 2626 2863 3742 4234 4532 535
Size: 1055 1036 237 879 492 298 1690
Fragments arranged by size:
1690 1055 1036 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 559 2321 5391 559
Size: 1762 3070 855
Fragments arranged by size:
3070 1762 855
BsaAI yAC'GTr
Cuts at: 605 801 3040 605
Size: 196 2239 3252
Fragments arranged by size:
3252 2239 196
BsaBI GATnn'nnATC
Cuts at: 6 3201 5238 6
Size: 3195 2037 455
Fragments arranged by size:
3195 2037 455
BsaHI Gr'CG_yC
Cuts at: 377 2627 3118 3366 4068 5564 377
Size: 2250 491 248 702 1496 500
Fragments arranged by size:
2250 1496 702 500 491 248
BsaWI w'CCGG_w
Cuts at: 2876 3397 4075 4349 4496 4907 2876
Size: 521 678 274 147 411 3656
Fragments arranged by size:
3656 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 796 826 1301 1331 2439 2469 2602
Size: 30 345 30 475 30 1108 30 133
Cuts at: 2602 2632 421
Size: 30 3476
Fragments arranged by size:
3476 1108 475 345 133 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 559 604 846 1098 2149 2663 445
Size: 114 45 242 252 1051 514 3469
Fragments arranged by size:
3469 1051 514 252 242 114 45
BseYI C'CCAG_C
Cuts at: 276 788 1200 1804 1945 2032 3112 3870 4447
Size: 512 412 604 141 87 1080 758 577
Cuts at: 4447 4943 276
Size: 496 1020
Fragments arranged by size:
1080 1020 758 604 577 512 496 412
141 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 5687
BsiEI CG_ry'CG
Cuts at: 104 3229 3275 4483 104
Size: 3125 46 1208 1308
Fragments arranged by size:
3125 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 196 464 551 1995 2121 2452 2701 3479 3669
Size: 268 87 1444 126 331 249 778 190
Cuts at: 3669 4461 196
Size: 792 1422
Fragments arranged by size:
1444 1422 792 778 331 268 249 190
126 87
Bsp1286I G_dGCh'C
Cuts at: 167 196 464 551 889 1995 2077 2121 2269
Size: 29 268 87 338 1106 82 44 148
Cuts at: 2269 2452 2655 2701 3312 3405 3479 3669 3731
Size: 183 203 46 611 93 74 190 62
Cuts at: 3731 4461 167
Size: 730 1393
Fragments arranged by size:
1393 1106 730 611 338 268 203 190
183 148 93 87 82 74 62 46
44 29
BspEI T'CCGG_A
Cuts at: 4907 4907
Size: 5687
BspHI T'CATG_A
Cuts at: 4863 4863
Size: 5687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 848 2737 2951 3253 3634 848
Size: 1889 214 302 381 2901
Fragments arranged by size:
2901 1889 381 302 214
BsrBI CCG'CTC
Cuts at: 50 140 579 3979 4033 5517 5682 50
Size: 90 439 3400 54 1484 165 55
Fragments arranged by size:
3400 1484 439 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 716 1301 3599 716
Size: 585 2298 2804
Fragments arranged by size:
2804 2298 585
BsrFI r'CCGG_y
Cuts at: 111 3685 3866 4075 111
Size: 3574 181 209 1723
Fragments arranged by size:
3574 1723 209 181
BsrGI T'GTAC_A
Cuts at: 1703 1703
Size: 5687
BssHII G'CGCG_C
Cuts at: 440 440
Size: 5687
BssSI C'ACGA_G
Cuts at: 232 2666 3958 4316 232
Size: 2434 1292 358 1603
Fragments arranged by size:
2434 1603 1292 358
BstAPI GCAn_nnn'nTGC
Cuts at: 191 3064 191
Size: 2873 2814
BstBI TT'CG_AA
Cuts at: 4048 4048
Size: 5687
BstEII G'GTnAC_C
Cuts at: 2143 2143
Size: 5687
BstXI CCAn_nnnn'nTGG
Cuts at: 1051 2247 1051
Size: 1196 4491
BstYI r'GATC_y
Cuts at: 1 485 674 998 1636 2712 3179 3202 3537
Size: 484 189 324 638 1076 467 23 335
Cuts at: 3537 3783 4795 4881 4893 5239 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1076 1012 638 484 467 449 346 335
324 246 189 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1904 1904
Size: 5687
BtgI C'CryG_G
Cuts at: 167 830 1124 2655 167
Size: 663 294 1531 3199
Fragments arranged by size:
3199 1531 663 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 341 801 3637 3818 3981 341
Size: 460 2836 181 163 2047
Fragments arranged by size:
2836 2047 460 181 163
BtsI GCAGTG_nn'
Cuts at: 2930 2930
Size: 5687
DraI TTT'AAA
Cuts at: 2686 2686
Size: 5687
DrdI GACnn_nn'nnGTC
Cuts at: 3393 4251 3393
Size: 858 4829
EaeI y'GGCC_r
Cuts at: 768 911 1715 3272 3446 3837 3864 5209 768
Size: 143 804 1557 174 391 27 1345 1246
Fragments arranged by size:
1557 1345 1246 804 391 174 143 27
EagI C'GGCC_G
Cuts at: 3272 3272
Size: 5687
EarI CTCTTCn'nnn_
Cuts at: 613 1120 1331 1372 3710 3920 613
Size: 507 211 41 2338 210 2380
Fragments arranged by size:
2380 2338 507 211 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 973 3895 4205 4351 5497 196
Size: 777 2922 310 146 1146 386
Fragments arranged by size:
2922 1146 777 386 310 146
EcoICRI GAG'CTC
Cuts at: 462 549 1993 2699 462
Size: 87 1444 706 3450
Fragments arranged by size:
3450 1444 706 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 514 664 1042 1128 1435 2251 2406 3512
Size: 79 150 378 86 307 816 155 1106
Cuts at: 3512 3944 4691 4959 435
Size: 432 747 268 1163
Fragments arranged by size:
1163 1106 816 747 432 378 307 268
155 150 86 79
EcoO109I rG'GnC_Cy
Cuts at: 636 885 2578 2826 636
Size: 249 1693 248 3497
Fragments arranged by size:
3497 1693 249 248
EcoRI G'AATT_C
Cuts at: 1016 2691 1016
Size: 1675 4012
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2525 3706 2525
Size: 1181 4506
FspI TGC'GCA
Cuts at: 181 612 3468 181
Size: 431 2856 2400
Fragments arranged by size:
2856 2400 431
HaeII r_GCGC'y
Cuts at: 380 3121 3369 4391 5370 380
Size: 2741 248 1022 979 697
Fragments arranged by size:
2741 1022 979 697 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2390 2422 2439 2471 3529 3559 3561 3591 2390
Size: 32 17 32 1058 30 2 30 4486
Fragments arranged by size:
4486 1058 32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 2130 2726 2130
Size: 596 5091
HindIII A'AGCT_T
Cuts at: 1033 1033
Size: 5687
KasI G'GCGC_C
Cuts at: 376 3117 3365 376
Size: 2741 248 2698
Fragments arranged by size:
2741 2698 248
KpnI G_GTAC'C
Cuts at: 2707 2707
Size: 5687
MluI A'CGCG_T
Cuts at: 5120 5120
Size: 5687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 977 1673 4358 4542 977
Size: 696 2685 184 2122
Fragments arranged by size:
2685 2122 696 184
MscI TGG'CCA
Cuts at: 913 1717 3448 913
Size: 804 1731 3152
Fragments arranged by size:
3152 1731 804
MslI CAynn'nnrTG
Cuts at: 1228 1453 1483 1849 3676 3803 1228
Size: 225 30 366 1827 127 3112
Fragments arranged by size:
3112 1827 366 225 127 30
MspA1I CmG'CkG
Cuts at: 792 2403 2798 3112 3472 4485 4730 792
Size: 1611 395 314 360 1013 245 1749
Fragments arranged by size:
1749 1611 1013 395 360 314 245
NaeI GCC'GGC
Cuts at: 113 3868 113
Size: 3755 1932
NarI GG'CG_CC
Cuts at: 377 3118 3366 377
Size: 2741 248 2698
Fragments arranged by size:
2741 2698 248
NcoI C'CATG_G
Cuts at: 167 830 1124 2655 167
Size: 663 294 1531 3199
Fragments arranged by size:
3199 1531 663 294
NgoMIV G'CCGG_C
Cuts at: 111 3866 111
Size: 3755 1932
NspI r_CATG'y
Cuts at: 1704 1854 2740 3240 4147 1704
Size: 150 886 500 907 3244
Fragments arranged by size:
3244 907 886 500 150
PasI CC'CwG_GG
Cuts at: 2443 2443
Size: 5687
PciI A'CATG_T
Cuts at: 1700 4143 1700
Size: 2443 3244
PflMI CCAn_nnn'nTGG
Cuts at: 869 1065 869
Size: 196 5491
PmeI GTTT'AAAC
Cuts at: 2686 2686
Size: 5687
PmlI CAC'GTG
Cuts at: 605 801 605
Size: 196 5491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2634 4826 4858 2602
Size: 32 2192 32 3431
Fragments arranged by size:
3431 2192 32 32
PpuMI rG'GwC_Cy
Cuts at: 636 636
Size: 5687
PshAI GACnn'nnGTC
Cuts at: 2150 2150
Size: 5687
PspOMI G'GGCC_C
Cuts at: 885 885
Size: 5687
PspXI vC'TCGA_Gb
Cuts at: 316 316
Size: 5687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2533 2565 2533
Size: 32 5655
PstI C_TGCA'G
Cuts at: 2734 3419 2734
Size: 685 5002
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5687
PvuII CAG'CTG
Cuts at: 792 2403 3112 3472 792
Size: 1611 709 360 3007
Fragments arranged by size:
3007 1611 709 360
RsrII CG'GwC_CG
Cuts at: 3882 3882
Size: 5687
SacI G_AGCT'C
Cuts at: 464 551 1995 2701 464
Size: 87 1444 706 3450
Fragments arranged by size:
3450 1444 706 87
SalI G'TCGA_C
Cuts at: 2724 2724
Size: 5687
SapI GCTCTTCn'nnn_
Cuts at: 1331 3710 3920 1331
Size: 2379 210 3098
Fragments arranged by size:
3098 2379 210
SbfI CC_TGCA'GG
Cuts at: 2734 2734
Size: 5687
ScaI AGT'ACT
Cuts at: 355 5204 355
Size: 4849 838
SfcI C'TryA_G
Cuts at: 33 333 748 2409 2730 3415 4408 4599 33
Size: 300 415 1661 321 685 993 191 1121
Fragments arranged by size:
1661 1121 993 685 415 321 300 191
SfoI GGC'GCC
Cuts at: 378 3119 3367 378
Size: 2741 248 2698
Fragments arranged by size:
2741 2698 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
SmaI CCC'GGG
Cuts at: 374 2709 374
Size: 2335 3352
SmlI C'TyrA_G
Cuts at: 316 550 1030 1605 2532 2605 2842 3757 4249
Size: 234 480 575 927 73 237 915 492
Cuts at: 4249 4511 316
Size: 262 1492
Fragments arranged by size:
1492 927 915 575 492 480 262 237
234 73
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5687
SphI G_CATG'C
Cuts at: 2740 3240 2740
Size: 500 5187
StyI C'CwwG_G
Cuts at: 167 830 1124 1625 1748 2655 2821 3175 167
Size: 663 294 501 123 907 166 354 2679
Fragments arranged by size:
2679 907 663 501 354 294 166 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1987 3270 3403 3862 4071 1987
Size: 1283 133 459 209 3603
Fragments arranged by size:
3603 1283 459 209 133
TatI w'GTAC_w
Cuts at: 353 1539 1703 2369 5202 353
Size: 1186 164 666 2833 838
Fragments arranged by size:
2833 1186 838 666 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 796 828 1301 1333 5204 5236 421
Size: 32 343 32 473 32 3871 32 872
Fragments arranged by size:
3871 872 473 343 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1590 3484 1590
Size: 1894 3793
XbaI T'CTAG_A
Cuts at: 2718 2718
Size: 5687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 660 906 1208 1269 1979 3182 660
Size: 246 302 61 710 1203 3165
Fragments arranged by size:
3165 1203 710 302 246 61
XhoI C'TCGA_G
Cuts at: 316 316
Size: 5687
XmaI C'CCGG_G
Cuts at: 372 2707 372
Size: 2335 3352
XmnI GAAnn'nnTTC
Cuts at: 877 877
Size: 5687
ZraI GAC'GTC
Cuts at: 2628 2628
Size: 5687
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PsiI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI