Restriction map for
pF1KE4265
(Circular) (Six-base) MAPSORT of: pF1KE4265.seq Check: 7565 from: 1 to: 6299
pF1KE4265 6299 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 3337 3337
Size: 6299
Acc65I G'GTAC_C
Cuts at: 1181 1934 3315 1181
Size: 753 1381 4165
Fragments arranged by size:
4165 1381 753
AclI AA'CG_TT
Cuts at: 3080 6139 3080
Size: 3059 3240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1627 1798 3208 4124 4556 5303 5571 1268
Size: 359 171 1410 916 432 747 268 1996
Fragments arranged by size:
1996 1410 916 747 432 359 268 171
AflIII A'CryG_T
Cuts at: 2070 3651 4755 5732 2070
Size: 1581 1104 977 2637
Fragments arranged by size:
2637 1581 1104 977
AgeI A'CCGG_T
Cuts at: 4687 4687
Size: 6299
AhdI GACnn_n'nnGTC
Cuts at: 2552 2691 2552
Size: 139 6160
AleI CACnn'nnGTG
Cuts at: 1121 2252 1121
Size: 1131 5168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3098 3098
Size: 6299
AlwNI CAG_nnn'CTG
Cuts at: 252 384 1336 1768 3604 5171 252
Size: 132 952 432 1836 1567 1380
Fragments arranged by size:
1836 1567 1380 952 432 132
ApaLI G'TGCA_C
Cuts at: 5069 5069
Size: 6299
ApoI r'AATT_y
Cuts at: 176 1701 3303 5652 5908 176
Size: 1525 1602 2349 256 567
Fragments arranged by size:
2349 1602 1525 567 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6299
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6299
AvaI C'yCGr_G
Cuts at: 117 2717 3319 117
Size: 2600 602 3097
Fragments arranged by size:
3097 2600 602
AvrII C'CTAG_G
Cuts at: 2688 2688
Size: 6299
BamHI G'GATC_C
Cuts at: 3324 3324
Size: 6299
BanI G'GyrC_C
Cuts at: 1181 1934 2338 2412 3165 3315 3729 3977 4012
Size: 753 404 74 753 150 414 248 35
Cuts at: 4012 1181
Size: 3468
Fragments arranged by size:
3468 753 753 414 404 248 150 74
35
BanII G_rGCy'C
Cuts at: 186 948 1040 1172 2246 3097 3127 3313 4343
Size: 762 92 132 1074 851 30 186 1030
Cuts at: 4343 186
Size: 2142
Fragments arranged by size:
2142 1074 1030 851 762 186 132 92
30
BbeI G_GCGC'C
Cuts at: 3733 3981 3733
Size: 248 6051
BbsI GAAGACnn'nnnn_
Cuts at: 699 1633 2519 699
Size: 934 886 4479
Fragments arranged by size:
4479 934 886
BciVI GTATCCnnnnn_n'
Cuts at: 3003 4197 4958 6299 3003
Size: 1194 761 1341 3003
Fragments arranged by size:
3003 1341 1194 761
BclI T'GATC_A
Cuts at: 3819 3819
Size: 6299
BglII A'GATC_T
Cuts at: 691 3814 5851 691
Size: 3123 2037 1139
Fragments arranged by size:
3123 2037 1139
BlpI GC'TnA_GC
Cuts at: 3411 3411
Size: 6299
Bme1580I G_kGCm'C
Cuts at: 1149 3924 4017 5073 1149
Size: 2775 93 1056 2375
Fragments arranged by size:
2775 2375 1056 93
BmgBI CAC'GTC
Cuts at: 2855 2855
Size: 6299
BmrI ACTGGGnnnn_n'
Cuts at: 3511 3592 3927 6071 3511
Size: 81 335 2144 3739
Fragments arranged by size:
3739 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4666 4666
Size: 6299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 232 377 972 2189 2591 2986 3037 3114
Size: 3 145 595 1217 402 395 51 77
Cuts at: 3114 3179 229
Size: 65 3349
Fragments arranged by size:
3349 1217 595 402 395 145 77 65
51 3
Bpu10I CC'TnA_GC
Cuts at: 1177 2759 2915 1177
Size: 1582 156 4561
Fragments arranged by size:
4561 1582 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 2803 3475 4354 4846 5144 477
Size: 2326 672 879 492 298 1632
Fragments arranged by size:
2326 1632 879 672 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6003 6003
Size: 6299
BsaAI yAC'GTr
Cuts at: 3652 3652
Size: 6299
BsaBI GATnn'nnATC
Cuts at: 6 3813 5850 6
Size: 3807 2037 455
Fragments arranged by size:
3807 2037 455
BsaHI Gr'CG_yC
Cuts at: 2616 3730 3978 4680 6176 2616
Size: 1114 248 702 1496 2739
Fragments arranged by size:
2739 1496 1114 702 248
BsaWI w'CCGG_w
Cuts at: 3488 4009 4687 4961 5108 5519 3488
Size: 521 678 274 147 411 4268
Fragments arranged by size:
4268 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 681 711 2524 2554 681
Size: 30 1813 30 4426
Fragments arranged by size:
4426 1813 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 1885 2606 2667 3117 739
Size: 1146 721 61 450 3921
Fragments arranged by size:
3921 1146 721 450 61
BseYI C'CCAG_C
Cuts at: 1148 1375 3724 4482 5059 5555 1148
Size: 227 2349 758 577 496 1892
Fragments arranged by size:
2349 1892 758 577 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 2455 3202 659
Size: 1796 747 3756
Fragments arranged by size:
3756 1796 747
BsiEI CG_ry'CG
Cuts at: 104 3841 3887 5095 104
Size: 3737 46 1208 1308
Fragments arranged by size:
3737 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 186 1172 2204 2980 3097 3127 3313 4091 4281
Size: 986 1032 776 117 30 186 778 190
Cuts at: 4281 5073 186
Size: 792 1412
Fragments arranged by size:
1412 1032 986 792 778 776 190 186
117 30
BsmI GAATG_Cn'
Cuts at: 547 1023 1312 547
Size: 476 289 5534
Fragments arranged by size:
5534 476 289
BsmBI CGTCTCn'nnnn_
Cuts at: 280 280
Size: 6299
Bsp1286I G_dGCh'C
Cuts at: 186 948 1040 1149 1172 2204 2246 2980 3097
Size: 762 92 109 23 1032 42 734 117
Cuts at: 3097 3127 3313 3924 4017 4091 4281 4343 5073
Size: 30 186 611 93 74 190 62 730
Cuts at: 5073 186
Size: 1412
Fragments arranged by size:
1412 1032 762 734 730 611 190 186
117 109 93 92 74 62 42 30
23
BspEI T'CCGG_A
Cuts at: 5519 5519
Size: 6299
BspHI T'CATG_A
Cuts at: 558 885 1427 5475 558
Size: 327 542 4048 1382
Fragments arranged by size:
4048 1382 542 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3222 3258 3349 3563 3865 4246 3222
Size: 36 91 214 302 381 5275
Fragments arranged by size:
5275 381 302 214 91 36
BsrBI CCG'CTC
Cuts at: 50 2649 2763 4591 4645 6129 6294 50
Size: 2599 114 1828 54 1484 165 55
Fragments arranged by size:
2599 1828 1484 165 114 55 54
BsrDI GCAATG_nn'
Cuts at: 2640 4211 2640
Size: 1571 4728
BsrFI r'CCGG_y
Cuts at: 2804 4297 4478 4687 2804
Size: 1493 181 209 4416
Fragments arranged by size:
4416 1493 209 181
BssSI C'ACGA_G
Cuts at: 3120 4570 4928 3120
Size: 1450 358 4491
Fragments arranged by size:
4491 1450 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2989 3676 2989
Size: 687 5612
BstBI TT'CG_AA
Cuts at: 4660 4660
Size: 6299
BstEII G'GTnAC_C
Cuts at: 1278 1278
Size: 6299
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2233
Size: 6299
BstYI r'GATC_y
Cuts at: 1 249 691 3324 3791 3814 4149 4395 5407
Size: 248 442 2633 467 23 335 246 1012
Cuts at: 5407 5493 5505 5851 1
Size: 86 12 346 449
Fragments arranged by size:
2633 1012 467 449 442 346 335 248
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1817 3088 3202 1817
Size: 1271 114 4914
Fragments arranged by size:
4914 1271 114
BtgI C'CryG_G
Cuts at: 1791 2736 2781 3053 1791
Size: 945 45 272 5037
Fragments arranged by size:
5037 945 272 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1365 1988 4249 4430 4593 1365
Size: 623 2261 181 163 3071
Fragments arranged by size:
3071 2261 623 181 163
BtsI GCAGTG_nn'
Cuts at: 2502 3542 2502
Size: 1040 5259
DraI TTT'AAA
Cuts at: 528 3298 528
Size: 2770 3529
DrdI GACnn_nn'nnGTC
Cuts at: 4005 4863 4005
Size: 858 5441
EaeI y'GGCC_r
Cuts at: 2003 2637 2778 3884 4058 4449 4476 5821 2003
Size: 634 141 1106 174 391 27 1345 2481
Fragments arranged by size:
2481 1345 1106 634 391 174 141 27
EagI C'GGCC_G
Cuts at: 3884 3884
Size: 6299
EarI CTCTTCn'nnn_
Cuts at: 1223 1386 4322 4532 1223
Size: 163 2936 210 2990
Fragments arranged by size:
2990 2936 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4507 4817 4963 6109 4507
Size: 310 146 1146 4697
Fragments arranged by size:
4697 1146 310 146
EcoICRI GAG'CTC
Cuts at: 184 1170 3095 3125 3311 184
Size: 986 1925 30 186 3172
Fragments arranged by size:
3172 1925 986 186 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 232 377 972 1268 1627 1798 2189 2591
Size: 3 145 595 296 359 171 391 402
Cuts at: 2591 2986 3037 3114 3179 3208 4124 4556 5303
Size: 395 51 77 65 29 916 432 747
Cuts at: 5303 5571 229
Size: 268 957
Fragments arranged by size:
957 916 747 595 432 402 395 391
359 296 268 171 145 77 65 51
29 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3242 3242
Size: 6299
EcoO109I rG'GnC_Cy
Cuts at: 1502 2693 2721 3247 3438 1502
Size: 1191 28 526 191 4363
Fragments arranged by size:
4363 1191 526 191 28
EcoRI G'AATT_C
Cuts at: 3303 3303
Size: 6299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4318 4318
Size: 6299
FspI TGC'GCA
Cuts at: 4080 4080
Size: 6299
HaeII r_GCGC'y
Cuts at: 1879 3733 3981 5003 5982 1879
Size: 1854 248 1022 979 2196
Fragments arranged by size:
2196 1854 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1096 1128 1744 1776 2041 2073 2506 2524 2538
Size: 32 616 32 265 32 433 18 14
Cuts at: 2538 2556 2570 2677 2709 2898 2930 4141 4171
Size: 18 14 107 32 189 32 1211 30
Cuts at: 4171 4173 4203 1096
Size: 2 30 3192
Fragments arranged by size:
3192 1211 616 433 265 189 107 32
32 32 32 32 30 30 18 18
14 14 2
HincII GTy'rAC
Cuts at: 2685 3338 2685
Size: 653 5646
KasI G'GCGC_C
Cuts at: 3729 3977 3729
Size: 248 6051
KpnI G_GTAC'C
Cuts at: 1185 1938 3319 1185
Size: 753 1381 4165
Fragments arranged by size:
4165 1381 753
MfeI C'AATT_G
Cuts at: 2909 2909
Size: 6299
MluI A'CGCG_T
Cuts at: 5732 5732
Size: 6299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 576 3295 4970 5154 576
Size: 2719 1675 184 1721
Fragments arranged by size:
2719 1721 1675 184
MscI TGG'CCA
Cuts at: 2005 2639 2780 4060 2005
Size: 634 141 1280 4244
Fragments arranged by size:
4244 1280 634 141
MslI CAynn'nnrTG
Cuts at: 1066 1121 2075 2252 4288 4415 1066
Size: 55 954 177 2036 127 2950
Fragments arranged by size:
2950 2036 954 177 127 55
MspA1I CmG'CkG
Cuts at: 3186 3410 3724 4084 5097 5342 3186
Size: 224 314 360 1013 245 4143
Fragments arranged by size:
4143 1013 360 314 245 224
NaeI GCC'GGC
Cuts at: 2806 4480 2806
Size: 1674 4625
NarI GG'CG_CC
Cuts at: 3730 3978 3730
Size: 248 6051
NcoI C'CATG_G
Cuts at: 2781 2781
Size: 6299
NdeI CA'TA_TG
Cuts at: 614 614
Size: 6299
NgoMIV G'CCGG_C
Cuts at: 2804 4478 2804
Size: 1674 4625
NspI r_CATG'y
Cuts at: 2074 2665 2992 3352 3852 4759 2074
Size: 591 327 360 500 907 3614
Fragments arranged by size:
3614 907 591 500 360 327
PasI CC'CwG_GG
Cuts at: 1506 3264 1506
Size: 1758 4541
PciI A'CATG_T
Cuts at: 2070 4755 2070
Size: 2685 3614
PfoI T'CCnGG_A
Cuts at: 245 298 245
Size: 53 6246
PmeI GTTT'AAAC
Cuts at: 3298 3298
Size: 6299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5438 5470 5438
Size: 32 6267
PpuMI rG'GwC_Cy
Cuts at: 1502 2693 3247 1502
Size: 1191 554 4554
Fragments arranged by size:
4554 1191 554
PsiI TTA'TAA
Cuts at: 1997 1997
Size: 6299
PstI C_TGCA'G
Cuts at: 1499 2732 3346 4031 1499
Size: 1233 614 685 3767
Fragments arranged by size:
3767 1233 685 614
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6299
PvuII CAG'CTG
Cuts at: 3186 3724 4084 3186
Size: 538 360 5401
Fragments arranged by size:
5401 538 360
RsrII CG'GwC_CG
Cuts at: 4494 4494
Size: 6299
SacI G_AGCT'C
Cuts at: 186 1172 3097 3127 3313 186
Size: 986 1925 30 186 3172
Fragments arranged by size:
3172 1925 986 186 30
SalI G'TCGA_C
Cuts at: 3336 3336
Size: 6299
SanDI GG'GwC_CC
Cuts at: 2693 2693
Size: 6299
SapI GCTCTTCn'nnn_
Cuts at: 1386 4322 4532 1386
Size: 2936 210 3153
Fragments arranged by size:
3153 2936 210
SbfI CC_TGCA'GG
Cuts at: 3346 3346
Size: 6299
ScaI AGT'ACT
Cuts at: 877 3147 5816 877
Size: 2270 2669 1360
Fragments arranged by size:
2669 2270 1360
SfcI C'TryA_G
Cuts at: 33 1495 2728 3342 4027 5020 5211 33
Size: 1462 1233 614 685 993 191 1121
Fragments arranged by size:
1462 1233 1121 993 685 614 191
SfoI GGC'GCC
Cuts at: 3731 3979 3731
Size: 248 6051
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6299
SmaI CCC'GGG
Cuts at: 2719 3321 2719
Size: 602 5697
SmlI C'TyrA_G
Cuts at: 456 2818 3454 4369 4861 5123 456
Size: 2362 636 915 492 262 1632
Fragments arranged by size:
2362 1632 915 636 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6299
SphI G_CATG'C
Cuts at: 2992 3352 3852 2992
Size: 360 500 5439
Fragments arranged by size:
5439 500 360
SspI AAT'ATT
Cuts at: 295 793 1444 295
Size: 498 651 5150
Fragments arranged by size:
5150 651 498
StuI AGG'CCT
Cuts at: 2775 2775
Size: 6299
StyI C'CwwG_G
Cuts at: 2528 2688 2781 3433 3787 2528
Size: 160 93 652 354 5040
Fragments arranged by size:
5040 652 354 160 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1111 1905 3183 3882 4015 4474 4683 1111
Size: 794 1278 699 133 459 209 2727
Fragments arranged by size:
2727 1278 794 699 459 209 133
TatI w'GTAC_w
Cuts at: 875 971 1967 3145 5814 875
Size: 96 996 1178 2669 1360
Fragments arranged by size:
2669 1360 1178 996 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 681 713 1309 1341 5816 5848 681
Size: 32 596 32 4475 32 1132
Fragments arranged by size:
4475 1132 596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4096 4096
Size: 6299
XbaI T'CTAG_A
Cuts at: 306 3330 306
Size: 3024 3275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 401 3794 401
Size: 3393 2906
XmaI C'CCGG_G
Cuts at: 2717 3319 2717
Size: 602 5697
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmtI BsiWI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI