Restriction map for
pF1KE4276
(Circular) (Six-base) MAPSORT of: pF1KE4276.seq Check: 5437 from: 1 to: 6896
pF1KE4276 6896 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 2333 2333
Size: 6896
AccI GT'mk_AC
Cuts at: 2211 3934 2211
Size: 1723 5173
Acc65I G'GTAC_C
Cuts at: 3912 3912
Size: 6896
AclI AA'CG_TT
Cuts at: 2868 6736 2868
Size: 3868 3028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1565 1862 2900 3521 4721 5153 5900 6168
Size: 18 297 1038 621 1200 432 747 268
Cuts at: 6168 1547
Size: 2275
Fragments arranged by size:
2275 1200 1038 747 621 432 297 268
18
AflII C'TTAA_G
Cuts at: 207 207
Size: 6896
AflIII A'CryG_T
Cuts at: 278 2421 4248 5352 6329 278
Size: 2143 1827 1104 977 845
Fragments arranged by size:
2143 1827 1104 977 845
AgeI A'CCGG_T
Cuts at: 5284 5284
Size: 6896
AlwNI CAG_nnn'CTG
Cuts at: 891 1419 1885 4201 5768 891
Size: 528 466 2316 1567 2019
Fragments arranged by size:
2316 2019 1567 528 466
ApaLI G'TGCA_C
Cuts at: 3407 5666 3407
Size: 2259 4637
ApoI r'AATT_y
Cuts at: 669 863 970 1334 1501 1518 2061 2122 2219
Size: 194 107 364 167 17 543 61 97
Cuts at: 2219 2305 2392 2501 2547 2933 3234 3310 3344
Size: 86 87 109 46 386 301 76 34
Cuts at: 3344 3875 3900 6249 6505 669
Size: 531 25 2349 256 1060
Fragments arranged by size:
2349 1060 543 531 386 364 301 256
194 167 109 107 97 87 86 76
61 46 34 25 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 6896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6896
AvaI C'yCGr_G
Cuts at: 605 3916 605
Size: 3311 3585
BamHI G'GATC_C
Cuts at: 3921 3921
Size: 6896
BanI G'GyrC_C
Cuts at: 3912 4326 4574 4609 3912
Size: 414 248 35 6199
Fragments arranged by size:
6199 414 248 35
BanII G_rGCy'C
Cuts at: 200 3910 4940 200
Size: 3710 1030 2156
Fragments arranged by size:
3710 2156 1030
BbeI G_GCGC'C
Cuts at: 4330 4578 4330
Size: 248 6648
BbsI GAAGACnn'nnnn_
Cuts at: 1130 1163 2468 2551 3527 3788 3866 1130
Size: 33 1305 83 976 261 78 4160
Fragments arranged by size:
4160 1305 976 261 83 78 33
BbvCI CC'TCA_GC
Cuts at: 3666 3666
Size: 6896
BciVI GTATCCnnnnn_n'
Cuts at: 2167 4794 5555 6896 2167
Size: 2627 761 1341 2167
Fragments arranged by size:
2627 2167 1341 761
BclI T'GATC_A
Cuts at: 2814 3395 4416 2814
Size: 581 1021 5294
Fragments arranged by size:
5294 1021 581
BfrBI ATG'CAT
Cuts at: 1103 3001 1103
Size: 1898 4998
BglII A'GATC_T
Cuts at: 1759 2759 4411 6448 1759
Size: 1000 1652 2037 2207
Fragments arranged by size:
2207 2037 1652 1000
BlpI GC'TnA_GC
Cuts at: 4008 4008
Size: 6896
Bme1580I G_kGCm'C
Cuts at: 3411 4521 4614 5670 3411
Size: 1110 93 1056 4637
Fragments arranged by size:
4637 1110 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3398 3473 4108 4189 4524 6668 3398
Size: 75 635 81 335 2144 3626
Fragments arranged by size:
3626 2144 635 335 81 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 5263 3152
Size: 2111 4785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1760 3397 3424 943
Size: 817 1637 27 4415
Fragments arranged by size:
4415 1637 817 27
Bpu10I CC'TnA_GC
Cuts at: 3083 3666 3083
Size: 583 6313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 4072 4951 5443 5741 184
Size: 3888 879 492 298 1339
Fragments arranged by size:
3888 1339 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3712 3822 6600 3712
Size: 110 2778 4008
Fragments arranged by size:
4008 2778 110
BsaAI yAC'GTr
Cuts at: 4249 4249
Size: 6896
BsaBI GATnn'nnATC
Cuts at: 6 3695 3812 4410 6447 6
Size: 3689 117 598 2037 455
Fragments arranged by size:
3689 2037 598 455 117
BsaHI Gr'CG_yC
Cuts at: 2330 4327 4575 5277 6773 2330
Size: 1997 248 702 1496 2453
Fragments arranged by size:
2453 1997 1496 702 248
BsaWI w'CCGG_w
Cuts at: 231 1349 4085 4606 5284 5558 5705 6116 231
Size: 1118 2736 521 678 274 147 411 1011
Fragments arranged by size:
2736 1118 1011 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1153 1183 2664 2694 1153
Size: 30 1481 30 5355
Fragments arranged by size:
5355 1481 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1174 1249 2685 3661 3805 1174
Size: 75 1436 976 144 4265
Fragments arranged by size:
4265 1436 976 144 75
BseYI C'CCAG_C
Cuts at: 4321 5079 5656 6152 4321
Size: 758 577 496 5065
Fragments arranged by size:
5065 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1172 1691 997
Size: 175 519 6202
Fragments arranged by size:
6202 519 175
BsiEI CG_ry'CG
Cuts at: 104 4438 4484 5692 104
Size: 4334 46 1208 1308
Fragments arranged by size:
4334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1738 3411 3570 3910 4688 4878 5670 1738
Size: 1673 159 340 778 190 792 2964
Fragments arranged by size:
2964 1673 792 778 340 190 159
BsmI GAATG_Cn'
Cuts at: 1080 2288 3851 1080
Size: 1208 1563 4125
Fragments arranged by size:
4125 1563 1208
BsmBI CGTCTCn'nnnn_
Cuts at: 3522 3522
Size: 6896
Bsp1286I G_dGCh'C
Cuts at: 200 1738 3411 3570 3910 4521 4614 4688 4878
Size: 1538 1673 159 340 611 93 74 190
Cuts at: 4878 4940 5670 200
Size: 62 730 1426
Fragments arranged by size:
1673 1538 1426 730 611 340 190 159
93 74 62
BspEI T'CCGG_A
Cuts at: 231 1349 6116 231
Size: 1118 4767 1011
Fragments arranged by size:
4767 1118 1011
BspHI T'CATG_A
Cuts at: 6072 6072
Size: 6896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1602 3946 4160 4462 4843 1602
Size: 2344 214 302 381 3655
Fragments arranged by size:
3655 2344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5188 5242 6726 6891 50
Size: 5138 54 1484 165 55
Fragments arranged by size:
5138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 582 4808 582
Size: 4226 2670
BsrFI r'CCGG_y
Cuts at: 4894 5075 5284 4894
Size: 181 209 6506
Fragments arranged by size:
6506 209 181
BsrGI T'GTAC_A
Cuts at: 2384 2384
Size: 6896
BssSI C'ACGA_G
Cuts at: 3709 5167 5525 3709
Size: 1458 358 5080
Fragments arranged by size:
5080 1458 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1419 4273 1075
Size: 344 2854 3698
Fragments arranged by size:
3698 2854 344
BstBI TT'CG_AA
Cuts at: 5257 5257
Size: 6896
BstXI CCAn_nnnn'nTGG
Cuts at: 2631 3448 2631
Size: 817 6079
BstYI r'GATC_y
Cuts at: 1 1759 1963 2414 2759 3921 4388 4411 4746
Size: 1758 204 451 345 1162 467 23 335
Cuts at: 4746 4992 6004 6090 6102 6448 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1758 1162 1012 467 451 449 346 345
335 246 204 86 23 12
BtgI C'CryG_G
Cuts at: 3702 3702
Size: 6896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4846 5027 5190 4846
Size: 181 163 6552
Fragments arranged by size:
6552 181 163
BtsI GCAGTG_nn'
Cuts at: 431 880 3577 3680 4139 431
Size: 449 2697 103 459 3188
Fragments arranged by size:
3188 2697 459 449 103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3104 3139 3104
Size: 35 6861
DraI TTT'AAA
Cuts at: 321 365 1565 3895 321
Size: 44 1200 2330 3322
Fragments arranged by size:
3322 2330 1200 44
DrdI GACnn_nn'nnGTC
Cuts at: 4602 5460 4602
Size: 858 6038
EaeI y'GGCC_r
Cuts at: 2473 4481 4655 5046 5073 6418 2473
Size: 2008 174 391 27 1345 2951
Fragments arranged by size:
2951 2008 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4481 4481
Size: 6896
EarI CTCTTCn'nnn_
Cuts at: 496 911 1151 1226 1408 1643 2018 4919 5129
Size: 415 240 75 182 235 375 2901 210
Cuts at: 5129 496
Size: 2263
Fragments arranged by size:
2901 2263 415 375 240 235 210 182
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1196 5104 5414 5560 6706 1196
Size: 3908 310 146 1146 1386
Fragments arranged by size:
3908 1386 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3908 3908
Size: 6896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1547 1565 1760 1862 2900 3397 3424 3521
Size: 604 18 195 102 1038 497 27 97
Cuts at: 3521 4721 5153 5900 6168 943
Size: 1200 432 747 268 1671
Fragments arranged by size:
1671 1200 1038 747 604 497 432 268
195 102 97 27 18
EcoO109I rG'GnC_Cy
Cuts at: 1684 2771 4035 1684
Size: 1087 1264 4545
Fragments arranged by size:
4545 1264 1087
EcoRI G'AATT_C
Cuts at: 863 2122 2501 3900 863
Size: 1259 379 1399 3859
Fragments arranged by size:
3859 1399 1259 379
EcoRV GAT'ATC
Cuts at: 261 2322 2907 261
Size: 2061 585 4250
Fragments arranged by size:
4250 2061 585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 816 4915 816
Size: 4099 2797
FspI TGC'GCA
Cuts at: 4677 4677
Size: 6896
HaeII r_GCGC'y
Cuts at: 4330 4578 5600 6579 4330
Size: 248 1022 979 4647
Fragments arranged by size:
4647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1153 1185 1645 1677 1759 1791 2639 2671 3031
Size: 32 460 32 82 32 848 32 360
Cuts at: 3031 3063 3517 3549 3727 3759 4738 4768 4770
Size: 32 454 32 178 32 979 30 2
Cuts at: 4770 4800 1153
Size: 30 3249
Fragments arranged by size:
3249 979 848 460 454 360 178 82
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 163 1398 3113 3772 3935 163
Size: 1235 1715 659 163 3124
Fragments arranged by size:
3124 1715 1235 659 163
HindIII A'AGCT_T
Cuts at: 793 2267 793
Size: 1474 5422
HpaI GTT'AAC
Cuts at: 1398 1398
Size: 6896
KasI G'GCGC_C
Cuts at: 4326 4574 4326
Size: 248 6648
KpnI G_GTAC'C
Cuts at: 3916 3916
Size: 6896
MfeI C'AATT_G
Cuts at: 947 2533 947
Size: 1586 5310
MluI A'CGCG_T
Cuts at: 6329 6329
Size: 6896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 239 1208 2442 5567 5751 239
Size: 969 1234 3125 184 1384
Fragments arranged by size:
3125 1384 1234 969 184
MscI TGG'CCA
Cuts at: 2475 4657 2475
Size: 2182 4714
MslI CAynn'nnrTG
Cuts at: 1999 2426 4885 5012 1999
Size: 427 2459 127 3883
Fragments arranged by size:
3883 2459 427 127
MspA1I CmG'CkG
Cuts at: 4007 4321 4681 5694 5939 4007
Size: 314 360 1013 245 4964
Fragments arranged by size:
4964 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5077 5077
Size: 6896
NarI GG'CG_CC
Cuts at: 4327 4575 4327
Size: 248 6648
NdeI CA'TA_TG
Cuts at: 738 3489 738
Size: 2751 4145
NgoMIV G'CCGG_C
Cuts at: 5075 5075
Size: 6896
NsiI A_TGCA'T
Cuts at: 1105 3003 1105
Size: 1898 4998
NspI r_CATG'y
Cuts at: 282 1107 1413 2425 3005 3619 3949 4449 5356
Size: 825 306 1012 580 614 330 500 907
Cuts at: 5356 282
Size: 1822
Fragments arranged by size:
1822 1012 907 825 614 580 500 330
306
PciI A'CATG_T
Cuts at: 278 2421 5352 278
Size: 2143 2931 1822
Fragments arranged by size:
2931 2143 1822
PflMI CCAn_nnn'nTGG
Cuts at: 1825 1825
Size: 6896
PfoI T'CCnGG_A
Cuts at: 494 1785 2094 3413 494
Size: 1291 309 1319 3977
Fragments arranged by size:
3977 1319 1291 309
PmeI GTTT'AAAC
Cuts at: 3895 3895
Size: 6896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 700 732 6035 6067 700
Size: 32 5303 32 1529
Fragments arranged by size:
5303 1529 32 32
PpuMI rG'GwC_Cy
Cuts at: 1684 2771 1684
Size: 1087 5809
PsiI TTA'TAA
Cuts at: 1696 3608 1696
Size: 1912 4984
PstI C_TGCA'G
Cuts at: 423 887 1424 1845 3584 3943 4628 423
Size: 464 537 421 1739 359 685 2691
Fragments arranged by size:
2691 1739 685 537 464 421 359
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6896
PvuII CAG'CTG
Cuts at: 4321 4681 4321
Size: 360 6536
RsrII CG'GwC_CG
Cuts at: 5091 5091
Size: 6896
SacI G_AGCT'C
Cuts at: 3910 3910
Size: 6896
SalI G'TCGA_C
Cuts at: 3933 3933
Size: 6896
SanDI GG'GwC_CC
Cuts at: 2771 2771
Size: 6896
SapI GCTCTTCn'nnn_
Cuts at: 911 4919 5129 911
Size: 4008 210 2678
Fragments arranged by size:
4008 2678 210
SbfI CC_TGCA'GG
Cuts at: 3943 3943
Size: 6896
ScaI AGT'ACT
Cuts at: 6413 6413
Size: 6896
SfcI C'TryA_G
Cuts at: 33 419 883 1420 1841 2212 3170 3337 3580
Size: 386 464 537 421 371 958 167 243
Cuts at: 3580 3939 4624 5617 5808 33
Size: 359 685 993 191 1121
Fragments arranged by size:
1121 993 958 685 537 464 421 386
371 359 243 191 167
SfoI GGC'GCC
Cuts at: 4328 4576 4328
Size: 248 6648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6896
SmaI CCC'GGG
Cuts at: 3918 3918
Size: 6896
SmlI C'TyrA_G
Cuts at: 199 207 605 4051 4966 5458 5720 199
Size: 8 398 3446 915 492 262 1375
Fragments arranged by size:
3446 1375 915 492 398 262 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6896
SphI G_CATG'C
Cuts at: 1107 3949 4449 1107
Size: 2842 500 3554
Fragments arranged by size:
3554 2842 500
StyI C'CwwG_G
Cuts at: 1899 2007 2665 3139 3832 4030 4384 1899
Size: 108 658 474 693 198 354 4411
Fragments arranged by size:
4411 693 658 474 354 198 108
SwaI ATTT'AAAT
Cuts at: 1565 1565
Size: 6896
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3585 4479 4612 5071 5280 3585
Size: 894 133 459 209 5201
Fragments arranged by size:
5201 894 459 209 133
TatI w'GTAC_w
Cuts at: 1493 2384 2574 6411 1493
Size: 891 190 3837 1978
Fragments arranged by size:
3837 1978 891 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1346 1378 1707 1739 2664 2696 6413 6445 1346
Size: 32 329 32 925 32 3717 32 1797
Fragments arranged by size:
3717 1797 925 329 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2651 4693 2651
Size: 2042 4854
XbaI T'CTAG_A
Cuts at: 342 1314 1756 1851 3030 3927 342
Size: 972 442 95 1179 897 3311
Fragments arranged by size:
3311 1179 972 897 442 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 822 4391 822
Size: 3569 3327
XhoI C'TCGA_G
Cuts at: 605 605
Size: 6896
XmaI C'CCGG_G
Cuts at: 3916 3916
Size: 6896
XmnI GAAnn'nnTTC
Cuts at: 329 672 2761 329
Size: 343 2089 4464
Fragments arranged by size:
4464 2089 343
ZraI GAC'GTC
Cuts at: 2331 2331
Size: 6896
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI
NcoI NheI NotI NruI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI