Restriction map for
pF1KE4277
(Circular) (Six-base) MAPSORT of: pF1KE4277.seq Check: 3708 from: 1 to: 6953
pF1KE4277 6953 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 591 3991 591
Size: 3400 3553
Acc65I G'GTAC_C
Cuts at: 567 3969 567
Size: 3402 3551
AclI AA'CG_TT
Cuts at: 6793 6793
Size: 6953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 948 1191 1638 2532 2842 3538 3742 4778
Size: 285 243 447 894 310 696 204 1036
Cuts at: 4778 5210 5957 6225 663
Size: 432 747 268 1391
Fragments arranged by size:
1391 1036 894 747 696 447 432 310
285 268 243 204
AfeI AGC'GCT
Cuts at: 2188 2188
Size: 6953
AflII C'TTAA_G
Cuts at: 945 945
Size: 6953
AflIII A'CryG_T
Cuts at: 4305 5409 6386 4305
Size: 1104 977 4872
Fragments arranged by size:
4872 1104 977
AgeI A'CCGG_T
Cuts at: 5341 5341
Size: 6953
AlwNI CAG_nnn'CTG
Cuts at: 1932 2100 2406 3600 3944 4258 5825 1932
Size: 168 306 1194 344 314 1567 3060
Fragments arranged by size:
3060 1567 1194 344 314 306 168
ApaLI G'TGCA_C
Cuts at: 1558 2804 5723 1558
Size: 1246 2919 2788
Fragments arranged by size:
2919 2788 1246
ApoI r'AATT_y
Cuts at: 1201 1459 1468 3928 3957 6306 6562 1201
Size: 258 9 2460 29 2349 256 1592
Fragments arranged by size:
2460 2349 1592 258 256 29 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 6953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6953
AvaI C'yCGr_G
Cuts at: 532 3170 3973 532
Size: 2638 803 3512
Fragments arranged by size:
3512 2638 803
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1699 1732 1699
Size: 33 6920
BamHI G'GATC_C
Cuts at: 260 1782 3978 260
Size: 1522 2196 3235
Fragments arranged by size:
3235 2196 1522
BanI G'GyrC_C
Cuts at: 550 567 3078 3166 3969 4383 4631 4666 550
Size: 17 2511 88 803 414 248 35 2837
Fragments arranged by size:
2837 2511 803 414 248 88 35 17
BanII G_rGCy'C
Cuts at: 116 838 3316 3967 4997 116
Size: 722 2478 651 1030 2072
Fragments arranged by size:
2478 2072 1030 722 651
BbeI G_GCGC'C
Cuts at: 554 4387 4635 554
Size: 3833 248 2872
Fragments arranged by size:
3833 2872 248
BbsI GAAGACnn'nnnn_
Cuts at: 2489 2993 3657 2489
Size: 504 664 5785
Fragments arranged by size:
5785 664 504
BciVI GTATCCnnnnn_n'
Cuts at: 1361 2054 4851 5612 6953 1361
Size: 693 2797 761 1341 1361
Fragments arranged by size:
2797 1361 1341 761 693
BclI T'GATC_A
Cuts at: 4473 4473
Size: 6953
BfrBI ATG'CAT
Cuts at: 2698 2698
Size: 6953
BglI GCCn_nnn'nGGC
Cuts at: 142 695 3253 142
Size: 553 2558 3842
Fragments arranged by size:
3842 2558 553
BglII A'GATC_T
Cuts at: 2201 4468 6505 2201
Size: 2267 2037 2649
Fragments arranged by size:
2649 2267 2037
BlpI GC'TnA_GC
Cuts at: 832 4065 832
Size: 3233 3720
Bme1580I G_kGCm'C
Cuts at: 1562 2808 3171 4578 4671 5727 1562
Size: 1246 363 1407 93 1056 2788
Fragments arranged by size:
2788 1407 1246 1056 363 93
BmrI ACTGGGnnnn_n'
Cuts at: 217 1741 2012 3134 3388 4165 4246 4581 6725
Size: 1524 271 1122 254 777 81 335 2144
Cuts at: 6725 217
Size: 445
Fragments arranged by size:
2144 1524 1122 777 445 335 271 254
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 201 3078 5320 201
Size: 2877 2242 1834
Fragments arranged by size:
2877 2242 1834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 289 583 2415 2650 3048 3288 3571 3646
Size: 93 294 1832 235 398 240 283 75
Cuts at: 3646 196
Size: 3503
Fragments arranged by size:
3503 1832 398 294 283 240 235 93
75
Bpu10I CC'TnA_GC
Cuts at: 2950 2950
Size: 6953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 1681 2669 4129 5008 5500 5798 1528
Size: 153 988 1460 879 492 298 2683
Fragments arranged by size:
2683 1460 988 879 492 298 153
BsaI GGTCTCn'nnnn_
Cuts at: 788 6657 788
Size: 5869 1084
BsaAI yAC'GTr
Cuts at: 3214 4306 3214
Size: 1092 5861
BsaBI GATnn'nnATC
Cuts at: 6 4467 6504 6
Size: 4461 2037 455
Fragments arranged by size:
4461 2037 455
BsaHI Gr'CG_yC
Cuts at: 551 4384 4632 5334 6830 551
Size: 3833 248 702 1496 674
Fragments arranged by size:
3833 1496 702 674 248
BsaWI w'CCGG_w
Cuts at: 127 2731 4142 4663 5341 5615 5762 6173 127
Size: 2604 1411 521 678 274 147 411 907
Fragments arranged by size:
2604 1411 907 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 133 163 262 292 370 400 133
Size: 30 99 30 78 30 6686
Fragments arranged by size:
6686 99 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 510 1552 2448 2574 3652 146
Size: 364 1042 896 126 1078 3447
Fragments arranged by size:
3447 1078 1042 896 364 126
BseYI C'CCAG_C
Cuts at: 293 1786 2832 3007 4378 5136 5713 6209 293
Size: 1493 1046 175 1371 758 577 496 1037
Fragments arranged by size:
1493 1371 1046 1037 758 577 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2812 3286 3598 2812
Size: 474 312 6167
Fragments arranged by size:
6167 474 312
BsiEI CG_ry'CG
Cuts at: 104 2324 4495 4541 5749 104
Size: 2220 2171 46 1208 1308
Fragments arranged by size:
2220 2171 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 116 1562 2794 2808 3967 4745 4935 5727 116
Size: 1446 1232 14 1159 778 190 792 1342
Fragments arranged by size:
1446 1342 1232 1159 792 778 190 14
BsmI GAATG_Cn'
Cuts at: 2143 2280 2143
Size: 137 6816
BsmBI CGTCTCn'nnnn_
Cuts at: 2403 2655 2403
Size: 252 6701
Bsp1286I G_dGCh'C
Cuts at: 116 838 1562 2794 2808 3171 3316 3967 4578
Size: 722 724 1232 14 363 145 651 611
Cuts at: 4578 4671 4745 4935 4997 5727 116
Size: 93 74 190 62 730 1342
Fragments arranged by size:
1342 1232 730 724 722 651 611 363
190 145 93 74 62 14
BspEI T'CCGG_A
Cuts at: 127 6173 127
Size: 6046 907
BspHI T'CATG_A
Cuts at: 1722 2147 6129 1722
Size: 425 3982 2546
Fragments arranged by size:
3982 2546 425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3348 3858 4003 4217 4519 4900 3348
Size: 510 145 214 302 381 5401
Fragments arranged by size:
5401 510 381 302 214 145
BsrBI CCG'CTC
Cuts at: 50 381 3642 3855 5245 5299 6783 6948 50
Size: 331 3261 213 1390 54 1484 165 55
Fragments arranged by size:
3261 1484 1390 331 213 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2918 3882 4865 2918
Size: 964 983 5006
Fragments arranged by size:
5006 983 964
BsrFI r'CCGG_y
Cuts at: 241 3278 3542 4951 5132 5341 241
Size: 3037 264 1409 181 209 1853
Fragments arranged by size:
3037 1853 1409 264 209 181
BssHII G'CGCG_C
Cuts at: 3833 3833
Size: 6953
BssSI C'ACGA_G
Cuts at: 741 785 1889 2348 5224 5582 741
Size: 44 1104 459 2876 358 2112
Fragments arranged by size:
2876 2112 1104 459 358 44
BstAPI GCAn_nnn'nTGC
Cuts at: 3078 3591 3600 4330 3078
Size: 513 9 730 5701
Fragments arranged by size:
5701 730 513 9
BstBI TT'CG_AA
Cuts at: 5314 5314
Size: 6953
BstEII G'GTnAC_C
Cuts at: 936 936
Size: 6953
BstXI CCAn_nnnn'nTGG
Cuts at: 561 636 2851 561
Size: 75 2215 4663
Fragments arranged by size:
4663 2215 75
BstYI r'GATC_y
Cuts at: 1 260 861 1782 2201 3137 3978 4445 4468
Size: 259 601 921 419 936 841 467 23
Cuts at: 4468 4803 5049 6061 6147 6159 6505 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 936 921 841 601 467 449 419
346 335 259 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 493 493
Size: 6953
BtgI C'CryG_G
Cuts at: 107 137 635 1938 2324 107
Size: 30 498 1303 386 4736
Fragments arranged by size:
4736 1303 498 386 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 501 4903 5084 5247 501
Size: 4402 181 163 2207
Fragments arranged by size:
4402 2207 181 163
BtsI GCAGTG_nn'
Cuts at: 641 1310 1933 2954 4196 641
Size: 669 623 1021 1242 3398
Fragments arranged by size:
3398 1242 1021 669 623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2982 3017 2982
Size: 35 6918
DraI TTT'AAA
Cuts at: 3952 3952
Size: 6953
DraIII CAC_nnn'GTG
Cuts at: 3078 3202 3078
Size: 124 6829
DrdI GACnn_nn'nnGTC
Cuts at: 217 4659 5517 217
Size: 4442 858 1653
Fragments arranged by size:
4442 1653 858
EaeI y'GGCC_r
Cuts at: 154 2102 2759 3254 4538 4712 5103 5130 6475
Size: 1948 657 495 1284 174 391 27 1345
Cuts at: 6475 154
Size: 632
Fragments arranged by size:
1948 1345 1284 657 632 495 391 174
27
EagI C'GGCC_G
Cuts at: 4538 4538
Size: 6953
EarI CTCTTCn'nnn_
Cuts at: 1226 3303 3434 3707 4976 5186 1226
Size: 2077 131 273 1269 210 2993
Fragments arranged by size:
2993 2077 1269 273 210 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5161 5471 5617 6763 5161
Size: 310 146 1146 5351
Fragments arranged by size:
5351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 114 3965 114
Size: 3851 3102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 289 583 663 948 1191 1638 2415 2532
Size: 93 294 80 285 243 447 777 117
Cuts at: 2532 2650 2842 3048 3288 3538 3571 3646 3742
Size: 118 192 206 240 250 33 75 96
Cuts at: 3742 4778 5210 5957 6225 196
Size: 1036 432 747 268 924
Fragments arranged by size:
1036 924 777 747 447 432 294 285
268 250 243 240 206 192 118 117
96 93 80 75 33
EcoNI CCTnn'n_nnAGG
Cuts at: 446 2035 446
Size: 1589 5364
EcoO109I rG'GnC_Cy
Cuts at: 204 581 1089 1393 2338 2822 4092 204
Size: 377 508 304 945 484 1270 3065
Fragments arranged by size:
3065 1270 945 508 484 377 304
EcoRI G'AATT_C
Cuts at: 1201 3928 3957 1201
Size: 2727 29 4197
Fragments arranged by size:
4197 2727 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4972 4972
Size: 6953
FspI TGC'GCA
Cuts at: 4734 4734
Size: 6953
HaeII r_GCGC'y
Cuts at: 148 175 554 690 2190 3528 4387 4635 5657
Size: 27 379 136 1500 1338 859 248 1022
Cuts at: 5657 6636 148
Size: 979 465
Fragments arranged by size:
1500 1338 1022 979 859 465 379 248
136 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 584 616 766 798 1803 1835 2044
Size: 32 419 32 150 32 1005 32 209
Cuts at: 2044 2058 2076 2090 2193 2206 2225 2238 2683
Size: 14 18 14 103 13 19 13 445
Cuts at: 2683 2715 4795 4825 4827 4857 133
Size: 32 2080 30 2 30 2229
Fragments arranged by size:
2229 2080 1005 445 419 209 150 103
32 32 32 32 32 30 30 19
18 14 14 13 13 2
HincII GTy'rAC
Cuts at: 1302 3992 1302
Size: 2690 4263
HindIII A'AGCT_T
Cuts at: 727 1748 3904 727
Size: 1021 2156 3776
Fragments arranged by size:
3776 2156 1021
KasI G'GCGC_C
Cuts at: 550 4383 4631 550
Size: 3833 248 2872
Fragments arranged by size:
3833 2872 248
KpnI G_GTAC'C
Cuts at: 571 3973 571
Size: 3402 3551
MfeI C'AATT_G
Cuts at: 1038 1038
Size: 6953
MluI A'CGCG_T
Cuts at: 6386 6386
Size: 6953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 233 2623 5624 5808 206
Size: 27 2390 3001 184 1351
Fragments arranged by size:
3001 2390 1351 184 27
MscI TGG'CCA
Cuts at: 2104 2761 4714 2104
Size: 657 1953 4343
Fragments arranged by size:
4343 1953 657
MslI CAynn'nnrTG
Cuts at: 3232 4942 5069 3232
Size: 1710 127 5116
Fragments arranged by size:
5116 1710 127
MspA1I CmG'CkG
Cuts at: 139 192 968 2775 3944 4064 4378 4738 5751
Size: 53 776 1807 1169 120 314 360 1013
Cuts at: 5751 5996 139
Size: 245 1096
Fragments arranged by size:
1807 1169 1096 1013 776 360 314 245
120 53
NaeI GCC'GGC
Cuts at: 3544 5134 3544
Size: 1590 5363
NarI GG'CG_CC
Cuts at: 551 4384 4632 551
Size: 3833 248 2872
Fragments arranged by size:
3833 2872 248
NcoI C'CATG_G
Cuts at: 107 635 107
Size: 528 6425
NgoMIV G'CCGG_C
Cuts at: 3542 5132 3542
Size: 1590 5363
NsiI A_TGCA'T
Cuts at: 2700 2700
Size: 6953
NspI r_CATG'y
Cuts at: 252 2698 3231 3487 3867 4006 4506 5413 252
Size: 2446 533 256 380 139 500 907 1792
Fragments arranged by size:
2446 1792 907 533 500 380 256 139
PciI A'CATG_T
Cuts at: 5409 5409
Size: 6953
PflMI CCAn_nnn'nTGG
Cuts at: 229 229
Size: 6953
PfoI T'CCnGG_A
Cuts at: 2666 2666
Size: 6953
PmeI GTTT'AAAC
Cuts at: 3952 3952
Size: 6953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6092 6124 6092
Size: 32 6921
PpuMI rG'GwC_Cy
Cuts at: 204 581 1089 1393 204
Size: 377 508 304 5764
Fragments arranged by size:
5764 508 377 304
PstI C_TGCA'G
Cuts at: 2471 4000 4685 2471
Size: 1529 685 4739
Fragments arranged by size:
4739 1529 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6953
PvuII CAG'CTG
Cuts at: 968 2775 3944 4378 4738 968
Size: 1807 1169 434 360 3183
Fragments arranged by size:
3183 1807 1169 434 360
RsrII CG'GwC_CG
Cuts at: 5148 5148
Size: 6953
SacI G_AGCT'C
Cuts at: 116 3967 116
Size: 3851 3102
SacII CC_GC'GG
Cuts at: 140 140
Size: 6953
SalI G'TCGA_C
Cuts at: 3990 3990
Size: 6953
SapI GCTCTTCn'nnn_
Cuts at: 4976 5186 4976
Size: 210 6743
SbfI CC_TGCA'GG
Cuts at: 4000 4000
Size: 6953
ScaI AGT'ACT
Cuts at: 1575 3123 6470 1575
Size: 1548 3347 2058
Fragments arranged by size:
3347 2058 1548
SfcI C'TryA_G
Cuts at: 33 2467 2927 3996 4681 5674 5865 33
Size: 2434 460 1069 685 993 191 1121
Fragments arranged by size:
2434 1121 1069 993 685 460 191
SfoI GGC'GCC
Cuts at: 552 4385 4633 552
Size: 3833 248 2872
Fragments arranged by size:
3833 2872 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6953
SgrAI Cr'CCGG_yG
Cuts at: 241 241
Size: 6953
SmaI CCC'GGG
Cuts at: 3975 3975
Size: 6953
SmlI C'TyrA_G
Cuts at: 945 1507 1696 2684 4108 5023 5515 5777 945
Size: 562 189 988 1424 915 492 262 2121
Fragments arranged by size:
2121 1424 988 915 562 492 262 189
SnaBI TAC'GTA
Cuts at: 3214 3214
Size: 6953
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6953
SphI G_CATG'C
Cuts at: 2698 3487 3867 4006 4506 2698
Size: 789 380 139 500 5145
Fragments arranged by size:
5145 789 500 380 139
SspI AAT'ATT
Cuts at: 1111 1179 2477 1111
Size: 68 1298 5587
Fragments arranged by size:
5587 1298 68
StuI AGG'CCT
Cuts at: 2923 2923
Size: 6953
StyI C'CwwG_G
Cuts at: 107 635 912 1085 1586 2084 4087 4441 107
Size: 528 277 173 501 498 2003 354 2619
Fragments arranged by size:
2619 2003 528 501 498 354 277 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 343 1386 2846 4536 4669 5128 5337 339
Size: 4 1043 1460 1690 133 459 209 1955
Fragments arranged by size:
1955 1690 1460 1043 459 209 133 4
TatI w'GTAC_w
Cuts at: 613 769 1360 1573 3121 6468 613
Size: 156 591 213 1548 3347 1098
Fragments arranged by size:
3347 1548 1098 591 213 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 6470 6502 262
Size: 32 6176 32 713
Fragments arranged by size:
6176 713 32 32
Tth111I GACn'n_nGTC
Cuts at: 4750 4750
Size: 6953
XbaI T'CTAG_A
Cuts at: 3468 3984 3468
Size: 516 6437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1440 2754 3546 4448 1440
Size: 1314 792 902 3945
Fragments arranged by size:
3945 1314 902 792
XmaI C'CCGG_G
Cuts at: 3973 3973
Size: 6953
XmnI GAAnn'nnTTC
Cuts at: 1007 1058 1111 1007
Size: 51 53 6849
Fragments arranged by size:
6849 53 51
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BbvCI BcgI BmgBI BmtI BsiWI BsrGI BstZ17I
ClaI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SrfI SwaI XhoI ZraI