Restriction map for
pF1KE4367
(Circular) (Six-base) MAPSORT of: pF1KE4367.seq Check: 4989 from: 1 to: 4271
pF1KE4367 4271 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1097 2096 2528 3275 3543 1097
Size: 999 432 747 268 1825
Fragments arranged by size:
1825 999 747 432 268
AfeI AGC'GCT
Cuts at: 147 147
Size: 4271
AflII C'TTAA_G
Cuts at: 220 220
Size: 4271
AflIII A'CryG_T
Cuts at: 577 864 1623 2727 3704 577
Size: 287 759 1104 977 1144
Fragments arranged by size:
1144 1104 977 759 287
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AhdI GACnn_n'nnGTC
Cuts at: 127 127
Size: 4271
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 576 608 576
Size: 32 4239
AlwNI CAG_nnn'CTG
Cuts at: 1005 1056 1075 1249 1576 3143 1005
Size: 51 19 174 327 1567 2133
Fragments arranged by size:
2133 1567 327 174 51 19
ApaLI G'TGCA_C
Cuts at: 402 868 3041 402
Size: 466 2173 1632
Fragments arranged by size:
2173 1632 466
ApoI r'AATT_y
Cuts at: 811 1275 3624 3880 811
Size: 464 2349 256 1202
Fragments arranged by size:
2349 1202 464 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 141 149 1291 141
Size: 8 1142 3121
Fragments arranged by size:
3121 1142 8
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 1287 1701 1949 1984 1287
Size: 414 248 35 3574
Fragments arranged by size:
3574 414 248 35
BanII G_rGCy'C
Cuts at: 1213 1285 2315 1213
Size: 72 1030 3169
Fragments arranged by size:
3169 1030 72
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BbsI GAAGACnn'nnnn_
Cuts at: 225 225
Size: 4271
BciVI GTATCCnnnnn_n'
Cuts at: 2169 2930 4271 2169
Size: 761 1341 2169
Fragments arranged by size:
2169 1341 761
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 406 872 1896 1989 3045 406
Size: 466 1024 93 1056 1632
Fragments arranged by size:
1632 1056 1024 466 93
BmgBI CAC'GTC
Cuts at: 578 578
Size: 4271
BmrI ACTGGGnnnn_n'
Cuts at: 683 1483 1564 1899 4043 683
Size: 800 81 335 2144 911
Fragments arranged by size:
2144 911 800 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1263 2638 1263
Size: 1375 2896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 407
Size: 4271
Bpu10I CC'TnA_GC
Cuts at: 840 840
Size: 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1447 2326 2818 3116 325
Size: 1122 879 492 298 1480
Fragments arranged by size:
1480 1122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 934 981 3975 934
Size: 47 2994 1230
Fragments arranged by size:
2994 1230 47
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 1702 1950 2652 4148 1702
Size: 248 702 1496 1825
Fragments arranged by size:
1825 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 1981 2659 2933 3080 3491 1460
Size: 521 678 274 147 411 2240
Fragments arranged by size:
2240 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 185 215 337 367 1108 1138 185
Size: 30 122 30 741 30 3318
Fragments arranged by size:
3318 741 122 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 364 709 866 1057 1104 1107 361
Size: 3 345 157 191 47 3 3525
Fragments arranged by size:
3525 345 191 157 47 3 3
BseYI C'CCAG_C
Cuts at: 302 684 1696 2454 3031 3527 302
Size: 382 1012 758 577 496 1046
Fragments arranged by size:
1046 1012 758 577 496 382
BsiEI CG_ry'CG
Cuts at: 104 137 1813 1859 3067 104
Size: 33 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 406 872 1285 2063 2253 3045 406
Size: 466 413 778 190 792 1632
Fragments arranged by size:
1632 792 778 466 413 190
Bsp1286I G_dGCh'C
Cuts at: 406 872 1213 1285 1896 1989 2063 2253 2315
Size: 466 341 72 611 93 74 190 62
Cuts at: 2315 3045 406
Size: 730 1632
Fragments arranged by size:
1632 730 611 466 341 190 93 74
72 62
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1321 1535 1837 2218 1321
Size: 214 302 381 3374
Fragments arranged by size:
3374 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 425 2183 425
Size: 1758 2513
BsrFI r'CCGG_y
Cuts at: 255 2269 2450 2659 255
Size: 2014 181 209 1867
Fragments arranged by size:
2014 1867 209 181
BsrGI T'GTAC_A
Cuts at: 460 460
Size: 4271
BssSI C'ACGA_G
Cuts at: 905 2542 2900 905
Size: 1637 358 2276
Fragments arranged by size:
2276 1637 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1648 1249
Size: 399 3872
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 603 714 603
Size: 111 4160
BstYI r'GATC_y
Cuts at: 1 215 364 1296 1763 1786 2121 2367 3379
Size: 214 149 932 467 23 335 246 1012
Cuts at: 3379 3465 3477 3823 1
Size: 86 12 346 449
Fragments arranged by size:
1012 932 467 449 346 335 246 214
149 86 23 12
BtgI C'CryG_G
Cuts at: 107 196 107
Size: 89 4182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 1514
Size: 4271
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DraIII CAC_nnn'GTG
Cuts at: 521 521
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 1032 1977 2835 1032
Size: 945 858 2468
Fragments arranged by size:
2468 945 858
EaeI y'GGCC_r
Cuts at: 1856 2030 2421 2448 3793 1856
Size: 174 391 27 1345 2334
Fragments arranged by size:
2334 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1856 1856
Size: 4271
EarI CTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 934 2479 2789 2935 4081 934
Size: 1545 310 146 1146 1124
Fragments arranged by size:
1545 1146 1124 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 1097 2096 2528 3275 3543 407
Size: 690 999 432 747 268 1135
Fragments arranged by size:
1135 999 747 690 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 343 343
Size: 4271
EcoO109I rG'GnC_Cy
Cuts at: 856 1410 856
Size: 554 3717
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 149 162 1705 1953 2975 3954 149
Size: 13 1543 248 1022 979 466
Fragments arranged by size:
1543 1022 979 466 248 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 185 217 541 573 590 622 1108
Size: 32 40 32 324 32 17 32 486
Cuts at: 1108 1140 2113 2143 2145 2175 113
Size: 32 973 30 2 30 2209
Fragments arranged by size:
2209 973 486 324 40 32 32 32
32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 267 1310 267
Size: 1043 3228
HindIII A'AGCT_T
Cuts at: 540 540
Size: 4271
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 784 2942 3126 458
Size: 326 2158 184 1603
Fragments arranged by size:
2158 1603 326 184
MscI TGG'CCA
Cuts at: 2032 2032
Size: 4271
MslI CAynn'nnrTG
Cuts at: 754 2260 2387 754
Size: 1506 127 2638
Fragments arranged by size:
2638 1506 127
MspA1I CmG'CkG
Cuts at: 661 691 1129 1382 1696 2056 3069 3314 661
Size: 30 438 253 314 360 1013 245 1618
Fragments arranged by size:
1618 1013 438 360 314 253 245 30
NaeI GCC'GGC
Cuts at: 2452 2452
Size: 4271
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 107 196 107
Size: 89 4182
NgoMIV G'CCGG_C
Cuts at: 2450 2450
Size: 4271
NspI r_CATG'y
Cuts at: 1180 1324 1824 2731 1180
Size: 144 500 907 2720
Fragments arranged by size:
2720 907 500 144
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PflMI CCAn_nnn'nTGG
Cuts at: 197 1206 197
Size: 1009 3262
PfoI T'CCnGG_A
Cuts at: 165 203 545 165
Size: 38 342 3891
Fragments arranged by size:
3891 342 38
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 856 856
Size: 4271
PspXI vC'TCGA_Gb
Cuts at: 141 149 141
Size: 8 4263
PstI C_TGCA'G
Cuts at: 1318 2003 1318
Size: 685 3586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 661 1696 2056 661
Size: 1035 360 2876
Fragments arranged by size:
2876 1035 360
RsrII CG'GwC_CG
Cuts at: 258 2466 258
Size: 2208 2063
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SfcI C'TryA_G
Cuts at: 33 1028 1314 1999 2992 3183 33
Size: 995 286 685 993 191 1121
Fragments arranged by size:
1121 995 993 685 286 191
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 141 149 220 340 1426 2341 2833 3095 141
Size: 8 71 120 1086 915 492 262 1317
Fragments arranged by size:
1317 1086 915 492 262 120 71 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 1324 1824 1324
Size: 500 3771
StyI C'CwwG_G
Cuts at: 107 196 993 1405 1759 107
Size: 89 797 412 354 2619
Fragments arranged by size:
2619 797 412 354 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 985 1854 1987 2446 2655 985
Size: 869 133 459 209 2601
Fragments arranged by size:
2601 869 459 209 133
TatI w'GTAC_w
Cuts at: 460 3786 460
Size: 3326 945
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 3788 3820 337
Size: 32 3419 32 788
Fragments arranged by size:
3419 788 32 32
Tth111I GACn'n_nGTC
Cuts at: 180 2068 180
Size: 1888 2383
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1766 1766
Size: 4271
XhoI C'TCGA_G
Cuts at: 141 149 141
Size: 8 4263
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
XmnI GAAnn'nnTTC
Cuts at: 811 1190 811
Size: 379 3892
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AleI AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI