Restriction map for
pF1KE4483
(Circular) (Six-base) MAPSORT of: pF1KE4483.seq Check: 333 from: 1 to: 4613
pF1KE4483 4613 bp
With 182 enzymes: SgfI *
November 17, 2015 09:53 ..
AccI GT'mk_AC
Cuts at: 1307 1651 1307
Size: 344 4269
Acc65I G'GTAC_C
Cuts at: 1629 1629
Size: 4613
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1158 2438 2870 3617 3885 1158
Size: 1280 432 747 268 1886
Fragments arranged by size:
1886 1280 747 432 268
AflIII A'CryG_T
Cuts at: 775 1965 3069 4046 775
Size: 1190 1104 977 1342
Fragments arranged by size:
1342 1190 1104 977
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AleI CACnn'nnGTG
Cuts at: 910 910
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 1251 1918 3485 1251
Size: 667 1567 2379
Fragments arranged by size:
2379 1567 667
ApaI G_GGCC'C
Cuts at: 698 698
Size: 4613
ApaLI G'TGCA_C
Cuts at: 996 3383 996
Size: 2387 2226
ApoI r'AATT_y
Cuts at: 1617 3966 4222 1617
Size: 2349 256 2008
Fragments arranged by size:
2349 2008 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 586 1121 1633 586
Size: 535 512 3566
Fragments arranged by size:
3566 535 512
BamHI G'GATC_C
Cuts at: 684 1638 684
Size: 954 3659
BanI G'GyrC_C
Cuts at: 1629 2043 2291 2326 1629
Size: 414 248 35 3916
Fragments arranged by size:
3916 414 248 35
BanII G_rGCy'C
Cuts at: 172 698 1205 1541 1627 2657 172
Size: 526 507 336 86 1030 2128
Fragments arranged by size:
2128 1030 526 507 336 86
BbeI G_GCGC'C
Cuts at: 2047 2295 2047
Size: 248 4365
BbsI GAAGACnn'nnnn_
Cuts at: 338 1164 1508 338
Size: 826 344 3443
Fragments arranged by size:
3443 826 344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1267 1301 1267
Size: 34 4579
BciVI GTATCCnnnnn_n'
Cuts at: 2511 3272 4613 2511
Size: 761 1341 2511
Fragments arranged by size:
2511 1341 761
BclI T'GATC_A
Cuts at: 2133 2133
Size: 4613
BglII A'GATC_T
Cuts at: 2128 4165 2128
Size: 2037 2576
BlpI GC'TnA_GC
Cuts at: 1725 1725
Size: 4613
Bme1580I G_kGCm'C
Cuts at: 698 1000 2238 2331 3387 698
Size: 302 1238 93 1056 1924
Fragments arranged by size:
1924 1238 1056 302 93
BmrI ACTGGGnnnn_n'
Cuts at: 1825 1906 2241 4385 1825
Size: 81 335 2144 2053
Fragments arranged by size:
2144 2053 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 749 1380 2980 749
Size: 631 1600 2382
Fragments arranged by size:
2382 1600 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1587 159
Size: 1428 3185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 1789 2668 3160 3458 810
Size: 979 879 492 298 1965
Fragments arranged by size:
1965 979 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1322 4317 1322
Size: 2995 1618
BsaAI yAC'GTr
Cuts at: 627 1966 627
Size: 1339 3274
BsaBI GATnn'nnATC
Cuts at: 6 1162 2127 4164 6
Size: 1156 965 2037 455
Fragments arranged by size:
2037 1156 965 455
BsaHI Gr'CG_yC
Cuts at: 452 2044 2292 2994 4490 452
Size: 1592 248 702 1496 575
Fragments arranged by size:
1592 1496 702 575 248
BsaWI w'CCGG_w
Cuts at: 735 1802 2323 3001 3275 3422 3833 735
Size: 1067 521 678 274 147 411 1515
Fragments arranged by size:
1515 1067 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 611 641 1528 1558 611
Size: 30 887 30 3666
Fragments arranged by size:
3666 887 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1330 1330
Size: 4613
BseYI C'CCAG_C
Cuts at: 1475 2038 2796 3373 3869 1475
Size: 563 758 577 496 2219
Fragments arranged by size:
2219 758 577 563 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 418 397
Size: 21 4592
BsiEI CG_ry'CG
Cuts at: 104 2155 2201 3409 104
Size: 2051 46 1208 1308
Fragments arranged by size:
2051 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1000 1541 1627 2405 2595 3387 1000
Size: 541 86 778 190 792 2226
Fragments arranged by size:
2226 792 778 541 190 86
BsmI GAATG_Cn'
Cuts at: 1290 1290
Size: 4613
Bsp1286I G_dGCh'C
Cuts at: 172 698 1000 1205 1541 1627 2238 2331 2405
Size: 526 302 205 336 86 611 93 74
Cuts at: 2405 2595 2657 3387 172
Size: 190 62 730 1398
Fragments arranged by size:
1398 730 611 526 336 302 205 190
93 86 74 62
BspEI T'CCGG_A
Cuts at: 735 3833 735
Size: 3098 1515
BspHI T'CATG_A
Cuts at: 3789 3789
Size: 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1663 1877 2179 2560 1663
Size: 214 302 381 3716
Fragments arranged by size:
3716 381 302 214
BsrBI CCG'CTC
Cuts at: 50 392 1272 2905 2959 4443 4608 50
Size: 342 880 1633 54 1484 165 55
Fragments arranged by size:
1633 1484 880 342 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 2525 539
Size: 1986 2627
BsrFI r'CCGG_y
Cuts at: 2611 2792 3001 2611
Size: 181 209 4223
Fragments arranged by size:
4223 209 181
BsrGI T'GTAC_A
Cuts at: 1495 1495
Size: 4613
BssSI C'ACGA_G
Cuts at: 421 2884 3242 421
Size: 2463 358 1792
Fragments arranged by size:
2463 1792 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1990 1990
Size: 4613
BstBI TT'CG_AA
Cuts at: 2974 2974
Size: 4613
BstXI CCAn_nnnn'nTGG
Cuts at: 528 528
Size: 4613
BstYI r'GATC_y
Cuts at: 1 684 1025 1340 1638 2105 2128 2463 2709
Size: 683 341 315 298 467 23 335 246
Cuts at: 2709 3721 3807 3819 4165 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 683 467 449 346 341 335 315
298 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2563 2744 2907 2563
Size: 181 163 4269
Fragments arranged by size:
4269 181 163
BtsI GCAGTG_nn'
Cuts at: 1856 1856
Size: 4613
DraI TTT'AAA
Cuts at: 1612 1612
Size: 4613
DrdI GACnn_nn'nnGTC
Cuts at: 2319 3177 2319
Size: 858 3755
EaeI y'GGCC_r
Cuts at: 312 629 2198 2372 2763 2790 4135 312
Size: 317 1569 174 391 27 1345 790
Fragments arranged by size:
1569 1345 790 391 317 174 27
EagI C'GGCC_G
Cuts at: 2198 2198
Size: 4613
EarI CTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1539 1625 1539
Size: 86 4527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1158 1587 2438 2870 3617 3885 159
Size: 999 429 851 432 747 268 887
Fragments arranged by size:
999 887 851 747 432 429 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1387 1387
Size: 4613
EcoO109I rG'GnC_Cy
Cuts at: 279 1361 1752 279
Size: 1082 391 3140
Fragments arranged by size:
3140 1082 391
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4613
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 2047 2295 3317 4296 2047
Size: 248 1022 979 2364
Fragments arranged by size:
2364 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 287 319 2455 2485 2487 2517 287
Size: 32 2136 30 2 30 2383
Fragments arranged by size:
2383 2136 32 30 30 2
HincII GTy'rAC
Cuts at: 471 954 1652 471
Size: 483 698 3432
Fragments arranged by size:
3432 698 483
KasI G'GCGC_C
Cuts at: 2043 2291 2043
Size: 248 4365
KpnI G_GTAC'C
Cuts at: 1633 1633
Size: 4613
MfeI C'AATT_G
Cuts at: 887 887
Size: 4613
MluI A'CGCG_T
Cuts at: 775 4046 775
Size: 3271 1342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3284 3468 3284
Size: 184 4429
MscI TGG'CCA
Cuts at: 2374 2374
Size: 4613
MslI CAynn'nnrTG
Cuts at: 910 1456 2602 2729 910
Size: 546 1146 127 2794
Fragments arranged by size:
2794 1146 546 127
MspA1I CmG'CkG
Cuts at: 1479 1724 2038 2398 3411 3656 1479
Size: 245 314 360 1013 245 2436
Fragments arranged by size:
2436 1013 360 314 245 245
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 4613
NarI GG'CG_CC
Cuts at: 2044 2292 2044
Size: 248 4365
NdeI CA'TA_TG
Cuts at: 894 894
Size: 4613
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 4613
NspI r_CATG'y
Cuts at: 1134 1666 2166 3073 1134
Size: 532 500 907 2674
Fragments arranged by size:
2674 907 532 500
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PflMI CCAn_nnn'nTGG
Cuts at: 862 862
Size: 4613
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PmlI CAC'GTG
Cuts at: 627 627
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 279 279
Size: 4613
PshAI GACnn'nnGTC
Cuts at: 238 238
Size: 4613
PspOMI G'GGCC_C
Cuts at: 694 694
Size: 4613
PstI C_TGCA'G
Cuts at: 340 566 1660 2345 340
Size: 226 1094 685 2608
Fragments arranged by size:
2608 1094 685 226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 2038 2398 2038
Size: 360 4253
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 1541 1627 1541
Size: 86 4527
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SfcI C'TryA_G
Cuts at: 33 336 562 609 1656 2341 3334 3525 33
Size: 303 226 47 1047 685 993 191 1121
Fragments arranged by size:
1121 1047 993 685 303 226 191 47
SfoI GGC'GCC
Cuts at: 2045 2293 2045
Size: 248 4365
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 1123 1635 1123
Size: 512 4101
SmlI C'TyrA_G
Cuts at: 789 1768 2683 3175 3437 789
Size: 979 915 492 262 1965
Fragments arranged by size:
1965 979 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 1134 1666 2166 1134
Size: 532 500 3581
Fragments arranged by size:
3581 532 500
StyI C'CwwG_G
Cuts at: 275 1747 2101 275
Size: 1472 354 2787
Fragments arranged by size:
2787 1472 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2196 2329 2788 2997 2196
Size: 133 459 209 3812
Fragments arranged by size:
3812 459 209 133
TatI w'GTAC_w
Cuts at: 1495 4128 1495
Size: 2633 1980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 611 643 4130 4162 611
Size: 32 3487 32 1062
Fragments arranged by size:
3487 1062 32 32
Tth111I GACn'n_nGTC
Cuts at: 2410 2410
Size: 4613
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 462 859 1299 1593 2108 462
Size: 397 440 294 515 2967
Fragments arranged by size:
2967 515 440 397 294
XmaI C'CCGG_G
Cuts at: 1121 1633 1121
Size: 512 4101
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NcoI
NheI NotI NruI NsiI PacI PasI PfoI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI