Restriction map for
pF1KE4526
(Circular) (Six-base) MAPSORT of: pF1KE4526.seq Check: 7232 from: 1 to: 4835
pF1KE4526 4835 bp
With 182 enzymes: SgfI *
March 8, 2012 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 246 246
Size: 4835
AccI GT'mk_AC
Cuts at: 1873 1873
Size: 4835
Acc65I G'GTAC_C
Cuts at: 1034 1851 1034
Size: 817 4018
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2660 3092 3839 4107 2660
Size: 432 747 268 3388
Fragments arranged by size:
3388 747 432 268
AfeI AGC'GCT
Cuts at: 310 1588 310
Size: 1278 3557
AflIII A'CryG_T
Cuts at: 2187 3291 4268 2187
Size: 1104 977 2754
Fragments arranged by size:
2754 1104 977
AgeI A'CCGG_T
Cuts at: 3223 3223
Size: 4835
AhdI GACnn_n'nnGTC
Cuts at: 687 687
Size: 4835
AleI CACnn'nnGTG
Cuts at: 1258 1258
Size: 4835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1827 1827
Size: 4835
AlwNI CAG_nnn'CTG
Cuts at: 1547 1565 1617 2140 3707 1547
Size: 18 52 523 1567 2675
Fragments arranged by size:
2675 1567 523 52 18
ApaI G_GGCC'C
Cuts at: 144 534 144
Size: 390 4445
ApaLI G'TGCA_C
Cuts at: 175 1711 3605 175
Size: 1536 1894 1405
Fragments arranged by size:
1894 1536 1405
ApoI r'AATT_y
Cuts at: 1839 4188 4444 1839
Size: 2349 256 2230
Fragments arranged by size:
2349 2230 256
AscI GG'CGCG_CC
Cuts at: 344 344
Size: 4835
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 119 196 276 326 527 797 838 871 991
Size: 77 80 50 201 270 41 33 120
Cuts at: 991 1060 1855 119
Size: 69 795 3099
Fragments arranged by size:
3099 795 270 201 120 80 77 69
50 41 33
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 154 396 455 477 651 952 1034 1851 2265
Size: 242 59 22 174 301 82 817 414
Cuts at: 2265 2513 2548 154
Size: 248 35 2441
Fragments arranged by size:
2441 817 414 301 248 242 174 82
59 35 22
BanII G_rGCy'C
Cuts at: 144 208 283 382 430 534 568 1272 1624
Size: 64 75 99 48 104 34 704 352
Cuts at: 1624 1849 2879 144
Size: 225 1030 2100
Fragments arranged by size:
2100 1030 704 352 225 104 99 75
64 48 34
BbeI G_GCGC'C
Cuts at: 158 400 655 2269 2517 158
Size: 242 255 1614 248 2476
Fragments arranged by size:
2476 1614 255 248 242
BbvCI CC'TCA_GC
Cuts at: 305 1168 305
Size: 863 3972
BciVI GTATCCnnnnn_n'
Cuts at: 2733 3494 4835 2733
Size: 761 1341 2733
Fragments arranged by size:
2733 1341 761
BclI T'GATC_A
Cuts at: 2355 2355
Size: 4835
BfrBI ATG'CAT
Cuts at: 1594 1594
Size: 4835
BglI GCCn_nnn'nGGC
Cuts at: 1336 1336
Size: 4835
BglII A'GATC_T
Cuts at: 2350 4387 2350
Size: 2037 2798
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 144 179 482 534 955 1399 1715 2460 2553
Size: 35 303 52 421 444 316 745 93
Cuts at: 2553 3609 144
Size: 1056 1370
Fragments arranged by size:
1370 1056 745 444 421 316 303 93
52 35
BmgBI CAC'GTC
Cuts at: 1683 1683
Size: 4835
BmrI ACTGGGnnnn_n'
Cuts at: 2047 2128 2463 4607 2047
Size: 81 335 2144 2275
Fragments arranged by size:
2275 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 3202 339
Size: 2863 1972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1573 1108
Size: 465 4370
Bpu10I CC'TnA_GC
Cuts at: 305 1168 1618 305
Size: 863 450 3522
Fragments arranged by size:
3522 863 450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 1690 2011 2890 3382 3680 1567
Size: 123 321 879 492 298 2722
Fragments arranged by size:
2722 879 492 321 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 585 1010 1216 4539 585
Size: 425 206 3323 881
Fragments arranged by size:
3323 881 425 206
BsaAI yAC'GTr
Cuts at: 291 885 1716 2188 291
Size: 594 831 472 2938
Fragments arranged by size:
2938 831 594 472
BsaBI GATnn'nnATC
Cuts at: 6 2349 4386 6
Size: 2343 2037 455
Fragments arranged by size:
2343 2037 455
BsaHI Gr'CG_yC
Cuts at: 155 387 397 652 2266 2514 3216 4712 155
Size: 232 10 255 1614 248 702 1496 278
Fragments arranged by size:
1614 1496 702 278 255 248 232 10
BsaWI w'CCGG_w
Cuts at: 2024 2545 3223 3497 3644 4055 2024
Size: 521 678 274 147 411 2804
Fragments arranged by size:
2804 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 1264 1291 1294 1321 550
Size: 30 684 27 3 27 4064
Fragments arranged by size:
4064 684 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 564 826 1315 564
Size: 262 489 4084
Fragments arranged by size:
4084 489 262
BseYI C'CCAG_C
Cuts at: 135 356 548 1376 2260 3018 3595 4091 135
Size: 221 192 828 884 758 577 496 879
Fragments arranged by size:
884 879 828 758 577 496 221 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 1705 1282
Size: 423 4412
BsiEI CG_ry'CG
Cuts at: 104 299 761 1206 2377 2423 3631 104
Size: 195 462 445 1171 46 1208 1308
Fragments arranged by size:
1308 1208 1171 462 445 195 46
BsiHKAI G_wGCw'C
Cuts at: 179 1715 1849 2627 2817 3609 179
Size: 1536 134 778 190 792 1405
Fragments arranged by size:
1536 1405 792 778 190 134
BsiWI C'GTAC_G
Cuts at: 1067 1067
Size: 4835
BsmBI CGTCTCn'nnnn_
Cuts at: 549 549
Size: 4835
Bsp1286I G_dGCh'C
Cuts at: 144 179 208 283 382 430 482 534 568
Size: 35 29 75 99 48 52 52 34
Cuts at: 568 955 1272 1399 1624 1715 1849 2460 2553
Size: 387 317 127 225 91 134 611 93
Cuts at: 2553 2627 2817 2879 3609 144
Size: 74 190 62 730 1370
Fragments arranged by size:
1370 730 611 387 317 225 190 134
127 99 93 91 75 74 62 52
52 48 35 34 29
BspEI T'CCGG_A
Cuts at: 4055 4055
Size: 4835
BspHI T'CATG_A
Cuts at: 4011 4011
Size: 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 246 1109 1885 2099 2401 2782 246
Size: 863 776 214 302 381 2299
Fragments arranged by size:
2299 863 776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1143 3127 3181 4665 4830 50
Size: 1093 1984 54 1484 165 55
Fragments arranged by size:
1984 1484 1093 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2747 2747
Size: 4835
BsrFI r'CCGG_y
Cuts at: 128 294 340 722 1104 1187 1418 2833 3014
Size: 166 46 382 382 83 231 1415 181
Cuts at: 3014 3223 128
Size: 209 1740
Fragments arranged by size:
1740 1415 382 382 231 209 181 166
83 46
BsrGI T'GTAC_A
Cuts at: 260 941 260
Size: 681 4154
BssHII G'CGCG_C
Cuts at: 344 802 1080 1082 1151 344
Size: 458 278 2 69 4028
Fragments arranged by size:
4028 458 278 69 2
BssSI C'ACGA_G
Cuts at: 3106 3464 3106
Size: 358 4477
BstAPI GCAn_nnn'nTGC
Cuts at: 2212 2212
Size: 4835
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1761 114
Size: 1647 3188
BstYI r'GATC_y
Cuts at: 1 1860 2327 2350 2685 2931 3943 4029 4041
Size: 1859 467 23 335 246 1012 86 12
Cuts at: 4041 4387 1
Size: 346 449
Fragments arranged by size:
1859 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 851 1334 851
Size: 483 4352
BtgI C'CryG_G
Cuts at: 107 372 728 761 1008 1206 1423 107
Size: 265 356 33 247 198 217 3519
Fragments arranged by size:
3519 356 265 247 217 198 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 2078 2078
Size: 4835
DraI TTT'AAA
Cuts at: 1834 1834
Size: 4835
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3399 2541
Size: 858 3977
EaeI y'GGCC_r
Cuts at: 296 338 444 471 500 758 1203 1313 1420
Size: 42 106 27 29 258 445 110 107
Cuts at: 1420 1523 2420 2594 2985 3012 4357 296
Size: 103 897 174 391 27 1345 774
Fragments arranged by size:
1345 897 774 445 391 258 174 110
107 106 103 42 29 27 27
EagI C'GGCC_G
Cuts at: 296 758 1203 2420 296
Size: 462 445 1217 2711
Fragments arranged by size:
2711 1217 462 445
EarI CTCTTCn'nnn_
Cuts at: 824 2858 3068 824
Size: 2034 210 2591
Fragments arranged by size:
2591 2034 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 558 1028 3043 3353 3499 4645 558
Size: 470 2015 310 146 1146 748
Fragments arranged by size:
2015 1146 748 470 310 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1573 2660 3092 3839 4107 1108
Size: 465 1087 432 747 268 1836
Fragments arranged by size:
1836 1087 747 465 432 268
EcoO109I rG'GnC_Cy
Cuts at: 140 141 878 1614 1974 140
Size: 1 737 736 360 3001
Fragments arranged by size:
3001 737 736 360 1
EcoRI G'AATT_C
Cuts at: 1839 1839
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4835
FspI TGC'GCA
Cuts at: 1111 2616 1111
Size: 1505 3330
HaeII r_GCGC'y
Cuts at: 158 312 400 655 1590 2269 2517 3539 4518
Size: 154 88 255 935 679 248 1022 979
Cuts at: 4518 158
Size: 475
Fragments arranged by size:
1022 979 935 679 475 255 248 154
88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 673 705 2677 2707 2709 2739 673
Size: 32 1972 30 2 30 2769
Fragments arranged by size:
2769 1972 32 30 30 2
HincII GTy'rAC
Cuts at: 1874 1874
Size: 4835
KasI G'GCGC_C
Cuts at: 154 396 651 2265 2513 154
Size: 242 255 1614 248 2476
Fragments arranged by size:
2476 1614 255 248 242
KpnI G_GTAC'C
Cuts at: 1038 1855 1038
Size: 817 4018
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1142 3506 3690 1142
Size: 2364 184 2287
Fragments arranged by size:
2364 2287 184
MscI TGG'CCA
Cuts at: 473 1315 2596 473
Size: 842 1281 2712
Fragments arranged by size:
2712 1281 842
MslI CAynn'nnrTG
Cuts at: 520 1258 2824 2951 520
Size: 738 1566 127 2404
Fragments arranged by size:
2404 1566 738 127
MspA1I CmG'CkG
Cuts at: 135 221 231 363 730 1208 1448 1946 2260
Size: 86 10 132 367 478 240 498 314
Cuts at: 2260 2620 3633 3878 135
Size: 360 1013 245 1092
Fragments arranged by size:
1092 1013 498 478 367 360 314 245
240 132 86 10
NaeI GCC'GGC
Cuts at: 130 296 342 3016 130
Size: 166 46 2674 1949
Fragments arranged by size:
2674 1949 166 46
NarI GG'CG_CC
Cuts at: 155 397 652 2266 2514 155
Size: 242 255 1614 248 2476
Fragments arranged by size:
2476 1614 255 248 242
NcoI C'CATG_G
Cuts at: 107 1008 107
Size: 901 3934
NgoMIV G'CCGG_C
Cuts at: 128 294 340 3014 128
Size: 166 46 2674 1949
Fragments arranged by size:
2674 1949 166 46
NsiI A_TGCA'T
Cuts at: 1596 1596
Size: 4835
NspI r_CATG'y
Cuts at: 1598 1888 2388 3295 1598
Size: 290 500 907 3138
Fragments arranged by size:
3138 907 500 290
PasI CC'CwG_GG
Cuts at: 1611 1611
Size: 4835
PciI A'CATG_T
Cuts at: 3291 3291
Size: 4835
PflMI CCAn_nnn'nTGG
Cuts at: 1470 1470
Size: 4835
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PmlI CAC'GTG
Cuts at: 1716 1716
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4803
PshAI GACnn'nnGTC
Cuts at: 986 986
Size: 4835
PspOMI G'GGCC_C
Cuts at: 140 530 140
Size: 390 4445
PstI C_TGCA'G
Cuts at: 1882 2567 1882
Size: 685 4150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 135 231 1448 2260 2620 135
Size: 96 1217 812 360 2350
Fragments arranged by size:
2350 1217 812 360 96
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SacII CC_GC'GG
Cuts at: 731 1209 731
Size: 478 4357
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 824 2858 3068 824
Size: 2034 210 2591
Fragments arranged by size:
2591 2034 210
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 4352 4352
Size: 4835
SfcI C'TryA_G
Cuts at: 33 1878 2563 3556 3747 33
Size: 1845 685 993 191 1121
Fragments arranged by size:
1845 1121 993 685 191
SfoI GGC'GCC
Cuts at: 156 398 653 2267 2515 156
Size: 242 255 1614 248 2476
Fragments arranged by size:
2476 1614 255 248 242
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 198 328 529 799 1857 198
Size: 130 201 270 1058 3176
Fragments arranged by size:
3176 1058 270 201 130
SmlI C'TyrA_G
Cuts at: 1582 1705 1990 2905 3397 3659 1582
Size: 123 285 915 492 262 2758
Fragments arranged by size:
2758 915 492 285 262 123
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1598 1888 2388 1598
Size: 290 500 4045
Fragments arranged by size:
4045 500 290
SrfI GCCC'GGGC
Cuts at: 198 529 198
Size: 331 4504
StuI AGG'CCT
Cuts at: 973 973
Size: 4835
StyI C'CwwG_G
Cuts at: 107 1008 1969 2323 107
Size: 901 961 354 2619
Fragments arranged by size:
2619 961 901 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 473 567 2418 2551 3010 3219 473
Size: 94 1851 133 459 209 2089
Fragments arranged by size:
2089 1851 459 209 133 94
TatI w'GTAC_w
Cuts at: 260 716 941 1277 4350 260
Size: 456 225 336 3073 745
Fragments arranged by size:
3073 745 456 336 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 550 582 1264 1291 1296 1323 4352 4384 550
Size: 32 682 27 5 27 3029 32 1001
Fragments arranged by size:
3029 1001 682 32 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 1222 2632 1222
Size: 1410 3425
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1028 1430 1548 2330 1028
Size: 402 118 782 3533
Fragments arranged by size:
3533 782 402 118
XmaI C'CCGG_G
Cuts at: 196 326 527 797 1855 196
Size: 130 201 270 1058 3176
Fragments arranged by size:
3176 1058 270 201 130
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AvrII BaeI BbsI BcgI BmtI
BsmI BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PfoI PpuMI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SspI SwaI XhoI XmnI
ZraI