Restriction map for
pF1KE4539
(Circular) (Six-base) MAPSORT of: pF1KE4539.seq Check: 6522 from: 1 to: 4979
pF1KE4539 4979 bp
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AatII G_ACGT'C
Cuts at: 1160 1279 1532 1160
Size: 119 253 4607
Fragments arranged by size:
4607 253 119
AccI GT'mk_AC
Cuts at: 820 2017 820
Size: 1197 3782
Acc65I G'GTAC_C
Cuts at: 1424 1995 1424
Size: 571 4408
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 1001 1312 1777 2804 3236 3983 4251 168
Size: 833 311 465 1027 432 747 268 896
Fragments arranged by size:
1027 896 833 747 465 432 311 268
AflIII A'CryG_T
Cuts at: 1505 1507 2331 3435 4412 1505
Size: 2 824 1104 977 2072
Fragments arranged by size:
2072 1104 977 824 2
AgeI A'CCGG_T
Cuts at: 3367 3367
Size: 4979
AhdI GACnn_n'nnGTC
Cuts at: 1010 1010
Size: 4979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 4979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 953 985 953
Size: 32 4947
AlwNI CAG_nnn'CTG
Cuts at: 591 1221 1478 1500 2284 3851 591
Size: 630 257 22 784 1567 1719
Fragments arranged by size:
1719 1567 784 630 257 22
ApaI G_GGCC'C
Cuts at: 509 1617 509
Size: 1108 3871
ApaLI G'TGCA_C
Cuts at: 3749 3749
Size: 4979
ApoI r'AATT_y
Cuts at: 1983 4332 4588 1983
Size: 2349 256 2374
Fragments arranged by size:
2374 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 207 442 501 564 577 741 935 1033 1201
Size: 235 59 63 13 164 194 98 168
Cuts at: 1201 1444 1899 1999 207
Size: 243 455 100 3187
Fragments arranged by size:
3187 455 243 235 194 168 164 100
98 63 59 13
AvrII C'CTAG_G
Cuts at: 1917 1917
Size: 4979
BamHI G'GATC_C
Cuts at: 1793 2004 1793
Size: 211 4768
BanI G'GyrC_C
Cuts at: 1424 1772 1995 2409 2657 2692 1424
Size: 348 223 414 248 35 3711
Fragments arranged by size:
3711 414 348 248 223 35
BanII G_rGCy'C
Cuts at: 440 509 576 660 1184 1249 1459 1617 1993
Size: 69 67 84 524 65 210 158 376
Cuts at: 1993 3023 440
Size: 1030 2396
Fragments arranged by size:
2396 1030 524 376 210 158 84 69
67 65
BbeI G_GCGC'C
Cuts at: 2413 2661 2413
Size: 248 4731
BbsI GAAGACnn'nnnn_
Cuts at: 1009 1009
Size: 4979
BbvCI CC'TCA_GC
Cuts at: 1438 1718 1879 1438
Size: 280 161 4538
Fragments arranged by size:
4538 280 161
BciVI GTATCCnnnnn_n'
Cuts at: 2877 3638 4979 2877
Size: 761 1341 2877
Fragments arranged by size:
2877 1341 761
BclI T'GATC_A
Cuts at: 2499 2499
Size: 4979
BglI GCCn_nnn'nGGC
Cuts at: 706 781 1102 706
Size: 75 321 4583
Fragments arranged by size:
4583 321 75
BglII A'GATC_T
Cuts at: 2494 4531 2494
Size: 2037 2942
BlpI GC'TnA_GC
Cuts at: 637 1453 2091 637
Size: 816 638 3525
Fragments arranged by size:
3525 816 638
Bme1580I G_kGCm'C
Cuts at: 509 1322 1466 1617 2604 2697 3753 509
Size: 813 144 151 987 93 1056 1735
Fragments arranged by size:
1735 1056 987 813 151 144 93
BmrI ACTGGGnnnn_n'
Cuts at: 2191 2272 2607 4751 2191
Size: 81 335 2144 2419
Fragments arranged by size:
2419 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 590 1473 1914 3346 590
Size: 883 441 1432 2223
Fragments arranged by size:
2223 1432 883 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 1303 242
Size: 1061 3918
Bpu10I CC'TnA_GC
Cuts at: 337 1438 1718 1879 337
Size: 1101 280 161 3437
Fragments arranged by size:
3437 1101 280 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2155 3034 3526 3824 1222
Size: 933 879 492 298 2377
Fragments arranged by size:
2377 933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 635 1279 4683 635
Size: 644 3404 931
Fragments arranged by size:
3404 931 644
BsaAI yAC'GTr
Cuts at: 1508 1746 2332 1508
Size: 238 586 4155
Fragments arranged by size:
4155 586 238
BsaBI GATnn'nnATC
Cuts at: 6 2493 4530 6
Size: 2487 2037 455
Fragments arranged by size:
2487 2037 455
BsaHI Gr'CG_yC
Cuts at: 455 1157 1276 1529 1906 2410 2658 3360 4856
Size: 702 119 253 377 504 248 702 1496
Cuts at: 4856 455
Size: 578
Fragments arranged by size:
1496 702 702 578 504 377 253 248
119
BsaWI w'CCGG_w
Cuts at: 2168 2689 3367 3641 3788 4199 2168
Size: 521 678 274 147 411 2948
Fragments arranged by size:
2948 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1417 1447 1417
Size: 30 4949
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 921 1462 1780 921
Size: 541 318 4120
Fragments arranged by size:
4120 541 318
BseYI C'CCAG_C
Cuts at: 614 870 1468 1827 1833 2404 3162 3739 4235
Size: 256 598 359 6 571 758 577 496
Cuts at: 4235 614
Size: 1358
Fragments arranged by size:
1358 758 598 577 571 496 359 256
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 501 1744 220
Size: 281 1243 3455
Fragments arranged by size:
3455 1243 281
BsiEI CG_ry'CG
Cuts at: 104 710 979 1356 2521 2567 3775 104
Size: 606 269 377 1165 46 1208 1308
Fragments arranged by size:
1308 1208 1165 606 377 269 46
BsiHKAI G_wGCw'C
Cuts at: 343 1184 1435 1459 1993 2771 2961 3753 343
Size: 841 251 24 534 778 190 792 1569
Fragments arranged by size:
1569 841 792 778 534 251 190 24
Bsp1286I G_dGCh'C
Cuts at: 343 440 509 576 660 1184 1249 1322 1435
Size: 97 69 67 84 524 65 73 113
Cuts at: 1435 1459 1466 1617 1993 2604 2697 2771 2961
Size: 24 7 151 376 611 93 74 190
Cuts at: 2961 3023 3753 343
Size: 62 730 1569
Fragments arranged by size:
1569 730 611 524 376 190 151 113
97 93 84 74 73 69 67 65
62 24 7
BspEI T'CCGG_A
Cuts at: 4199 4199
Size: 4979
BspHI T'CATG_A
Cuts at: 1676 4155 1676
Size: 2479 2500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 479 566 689 2029 2243 2545 2926 479
Size: 87 123 1340 214 302 381 2532
Fragments arranged by size:
2532 1340 381 302 214 123 87
BsrBI CCG'CTC
Cuts at: 50 1770 3271 3325 4809 4974 50
Size: 1720 1501 54 1484 165 55
Fragments arranged by size:
1720 1501 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2891 2891
Size: 4979
BsrFI r'CCGG_y
Cuts at: 425 2977 3158 3367 425
Size: 2552 181 209 2037
Fragments arranged by size:
2552 2037 209 181
BssSI C'ACGA_G
Cuts at: 3250 3608 3250
Size: 358 4621
BstAPI GCAn_nnn'nTGC
Cuts at: 2356 2356
Size: 4979
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstEII G'GTnAC_C
Cuts at: 1195 1195
Size: 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 307 1113 307
Size: 806 4173
BstYI r'GATC_y
Cuts at: 1 1592 1793 2004 2471 2494 2829 3075 4087
Size: 1591 201 211 467 23 335 246 1012
Cuts at: 4087 4173 4185 4531 1
Size: 86 12 346 449
Fragments arranged by size:
1591 1012 467 449 346 335 246 211
201 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1250 1334 1250
Size: 84 4895
BtgI C'CryG_G
Cuts at: 107 979 1619 107
Size: 872 640 3467
Fragments arranged by size:
3467 872 640
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2929 3110 3273 2929
Size: 181 163 4635
Fragments arranged by size:
4635 181 163
BtsI GCAGTG_nn'
Cuts at: 1948 2222 1948
Size: 274 4705
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 2685 3543 2685
Size: 858 4121
EaeI y'GGCC_r
Cuts at: 263 698 707 911 976 1103 1340 1667 1970
Size: 435 9 204 65 127 237 327 303
Cuts at: 1970 2564 2738 3129 3156 4501 263
Size: 594 174 391 27 1345 741
Fragments arranged by size:
1345 741 594 435 391 327 303 237
204 174 127 65 27 9
EagI C'GGCC_G
Cuts at: 707 976 2564 707
Size: 269 1588 3122
Fragments arranged by size:
3122 1588 269
EarI CTCTTCn'nnn_
Cuts at: 187 1237 3002 3212 187
Size: 1050 1765 210 1954
Fragments arranged by size:
1954 1765 1050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1777 3187 3497 3643 4789 1777
Size: 1410 310 146 1146 1967
Fragments arranged by size:
1967 1410 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1182 1457 1991 1182
Size: 275 534 4170
Fragments arranged by size:
4170 534 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 242 1001 1303 1312 1777 2804 3236 3983
Size: 74 759 302 9 465 1027 432 747
Cuts at: 3983 4251 168
Size: 268 896
Fragments arranged by size:
1027 896 759 747 465 432 302 268
74 9
EcoNI CCTnn'n_nnAGG
Cuts at: 754 754
Size: 4979
EcoO109I rG'GnC_Cy
Cuts at: 140 149 212 447 505 506 745 799 855
Size: 9 63 235 58 1 239 54 56
Cuts at: 855 939 1490 1497 1613 1614 1738 1752 1893
Size: 84 551 7 116 1 124 14 141
Cuts at: 1893 1921 2118 140
Size: 28 197 3001
Fragments arranged by size:
3001 551 239 235 197 141 124 116
84 63 58 56 54 28 14 9
7 1 1
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 4979
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4979
HaeII r_GCGC'y
Cuts at: 693 1639 2413 2661 3683 4662 693
Size: 946 774 248 1022 979 1010
Fragments arranged by size:
1022 1010 979 946 774 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 831 863 996 1028 1919 1951 2821
Size: 32 315 32 133 32 891 32 870
Cuts at: 2821 2851 2853 2883 484
Size: 30 2 30 2580
Fragments arranged by size:
2580 891 870 315 133 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 821 1311 1709 2018 821
Size: 490 398 309 3782
Fragments arranged by size:
3782 490 398 309
KasI G'GCGC_C
Cuts at: 2409 2657 2409
Size: 248 4731
KpnI G_GTAC'C
Cuts at: 1428 1999 1428
Size: 571 4408
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3650 3834 3650
Size: 184 4795
MscI TGG'CCA
Cuts at: 265 1105 1342 1669 2740 265
Size: 840 237 327 1071 2504
Fragments arranged by size:
2504 1071 840 327 237
MslI CAynn'nnrTG
Cuts at: 1111 1675 2968 3095 1111
Size: 564 1293 127 2995
Fragments arranged by size:
2995 1293 564 127
MspA1I CmG'CkG
Cuts at: 408 588 981 1152 1524 1806 2090 2404 2764
Size: 180 393 171 372 282 284 314 360
Cuts at: 2764 3777 4022 408
Size: 1013 245 1365
Fragments arranged by size:
1365 1013 393 372 360 314 284 282
245 180 171
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 4979
NarI GG'CG_CC
Cuts at: 2410 2658 2410
Size: 248 4731
NcoI C'CATG_G
Cuts at: 107 107
Size: 4979
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 4979
NspI r_CATG'y
Cuts at: 1887 2032 2532 3439 1887
Size: 145 500 907 3427
Fragments arranged by size:
3427 907 500 145
PasI CC'CwG_GG
Cuts at: 1420 1420
Size: 4979
PciI A'CATG_T
Cuts at: 3435 3435
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 1221 1500 1221
Size: 279 4700
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 4979
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PmlI CAC'GTG
Cuts at: 1508 1746 1508
Size: 238 4741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4947
PpuMI rG'GwC_Cy
Cuts at: 140 149 212 447 745 799 939 1497 1738
Size: 9 63 235 298 54 140 558 241
Cuts at: 1738 1921 140
Size: 183 3198
Fragments arranged by size:
3198 558 298 241 235 183 140 63
54 9
PspOMI G'GGCC_C
Cuts at: 505 1613 505
Size: 1108 3871
PspXI vC'TCGA_Gb
Cuts at: 207 207
Size: 4979
PstI C_TGCA'G
Cuts at: 791 2026 2711 791
Size: 1235 685 3059
Fragments arranged by size:
3059 1235 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 408 1152 1524 2404 2764 408
Size: 744 372 880 360 2623
Fragments arranged by size:
2623 880 744 372 360
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1184 1459 1993 1184
Size: 275 534 4170
Fragments arranged by size:
4170 534 275
SacII CC_GC'GG
Cuts at: 982 982
Size: 4979
SalI G'TCGA_C
Cuts at: 819 2016 819
Size: 1197 3782
SanDI GG'GwC_CC
Cuts at: 149 745 799 939 1738 149
Size: 596 54 140 799 3390
Fragments arranged by size:
3390 799 596 140 54
SapI GCTCTTCn'nnn_
Cuts at: 187 3002 3212 187
Size: 2815 210 1954
Fragments arranged by size:
2815 1954 210
SbfI CC_TGCA'GG
Cuts at: 2026 2026
Size: 4979
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SexAI A'CCwGG_T
Cuts at: 1865 1865
Size: 4979
SfcI C'TryA_G
Cuts at: 33 787 1840 2022 2707 3700 3891 33
Size: 754 1053 182 685 993 191 1121
Fragments arranged by size:
1121 1053 993 754 685 191 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 706 1102 706
Size: 396 4583
SfoI GGC'GCC
Cuts at: 2411 2659 2411
Size: 248 4731
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 743 2001 743
Size: 1258 3721
SmlI C'TyrA_G
Cuts at: 207 1237 2134 3049 3541 3803 207
Size: 1030 897 915 492 262 1383
Fragments arranged by size:
1383 1030 915 897 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 1887 2032 2532 1887
Size: 145 500 4334
Fragments arranged by size:
4334 500 145
StuI AGG'CCT
Cuts at: 514 668 514
Size: 154 4825
StyI C'CwwG_G
Cuts at: 107 306 371 1516 1541 1917 2113 2467 107
Size: 199 65 1145 25 376 196 354 2619
Fragments arranged by size:
2619 1145 376 354 199 196 65 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 186 1123 1597 2562 2695 3154 3363 186
Size: 937 474 965 133 459 209 1802
Fragments arranged by size:
1802 965 937 474 459 209 133
TatI w'GTAC_w
Cuts at: 4494 4494
Size: 4979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1730 1762 4496 4528 1730
Size: 32 2734 32 2181
Fragments arranged by size:
2734 2181 32 32
Tth111I GACn'n_nGTC
Cuts at: 256 825 2776 256
Size: 569 1951 2459
Fragments arranged by size:
2459 1951 569
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1863 2474 1863
Size: 611 4368
XhoI C'TCGA_G
Cuts at: 207 207
Size: 4979
XmaI C'CCGG_G
Cuts at: 741 1999 741
Size: 1258 3721
ZraI GAC'GTC
Cuts at: 1158 1277 1530 1158
Size: 119 253 4607
Fragments arranged by size:
4607 253 119
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AscI BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PsrI SgrAI SnaBI SrfI SspI SwaI XmnI