Restriction map for
pF1KE4564
(Circular) (Six-base) MAPSORT of: pF1KE4564.seq Check: 6070 from: 1 to: 5219
pF1KE4564 5219 bp
With 182 enzymes: SgfI *
January 12, 2017 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 912 1446 912
Size: 534 4685
AccI GT'mk_AC
Cuts at: 527 2257 527
Size: 1730 3489
Acc65I G'GTAC_C
Cuts at: 2235 2235
Size: 5219
AclI AA'CG_TT
Cuts at: 5059 5059
Size: 5219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3044 3476 4223 4491 3044
Size: 432 747 268 3772
Fragments arranged by size:
3772 747 432 268
AflIII A'CryG_T
Cuts at: 474 2571 3675 4652 474
Size: 2097 1104 977 1041
Fragments arranged by size:
2097 1104 1041 977
AgeI A'CCGG_T
Cuts at: 3607 3607
Size: 5219
AlwNI CAG_nnn'CTG
Cuts at: 1024 1597 2131 2524 4091 1024
Size: 573 534 393 1567 2152
Fragments arranged by size:
2152 1567 573 534 393
ApaI G_GGCC'C
Cuts at: 386 386
Size: 5219
ApaLI G'TGCA_C
Cuts at: 3989 3989
Size: 5219
ApoI r'AATT_y
Cuts at: 2223 4572 4828 2223
Size: 2349 256 2614
Fragments arranged by size:
2614 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5219
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
AvaI C'yCGr_G
Cuts at: 2239 2239
Size: 5219
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 300 333 300
Size: 33 5186
BamHI G'GATC_C
Cuts at: 991 2244 991
Size: 1253 3966
BanI G'GyrC_C
Cuts at: 2235 2649 2897 2932 2235
Size: 414 248 35 4522
Fragments arranged by size:
4522 414 248 35
BanII G_rGCy'C
Cuts at: 386 959 1493 2233 3263 386
Size: 573 534 740 1030 2342
Fragments arranged by size:
2342 1030 740 573 534
BbeI G_GCGC'C
Cuts at: 2653 2901 2653
Size: 248 4971
BbvCI CC'TCA_GC
Cuts at: 1405 1405
Size: 5219
BciVI GTATCCnnnnn_n'
Cuts at: 1994 3117 3878 5219 1994
Size: 1123 761 1341 1994
Fragments arranged by size:
1994 1341 1123 761
BclI T'GATC_A
Cuts at: 2739 2739
Size: 5219
BfrBI ATG'CAT
Cuts at: 421 421
Size: 5219
BglII A'GATC_T
Cuts at: 489 2734 4771 489
Size: 2245 2037 937
Fragments arranged by size:
2245 2037 937
BlpI GC'TnA_GC
Cuts at: 2331 2331
Size: 5219
Bme1580I G_kGCm'C
Cuts at: 386 2844 2937 3993 386
Size: 2458 93 1056 1612
Fragments arranged by size:
2458 1612 1056 93
BmgBI CAC'GTC
Cuts at: 477 477
Size: 5219
BmrI ACTGGGnnnn_n'
Cuts at: 760 1318 1726 1852 2431 2512 2847 4991 760
Size: 558 408 126 579 81 335 2144 988
Fragments arranged by size:
2144 988 579 558 408 335 126 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 1954 3586 135
Size: 1819 1632 1768
Fragments arranged by size:
1819 1768 1632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1608 540
Size: 1068 4151
Bpu10I CC'TnA_GC
Cuts at: 1405 1405
Size: 5219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2395 3274 3766 4064 2395
Size: 879 492 298 3550
Fragments arranged by size:
3550 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2158 4923 2158
Size: 2765 2454
BsaAI yAC'GTr
Cuts at: 1001 2572 1001
Size: 1571 3648
BsaBI GATnn'nnATC
Cuts at: 6 2733 4770 6
Size: 2727 2037 455
Fragments arranged by size:
2727 2037 455
BsaHI Gr'CG_yC
Cuts at: 2650 2898 3600 5096 2650
Size: 248 702 1496 2773
Fragments arranged by size:
2773 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2408 2929 3607 3881 4028 4439 2408
Size: 521 678 274 147 411 3188
Fragments arranged by size:
3188 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2180 2210 2180
Size: 30 5189
BseYI C'CCAG_C
Cuts at: 284 2161 2644 3402 3979 4475 284
Size: 1877 483 758 577 496 1028
Fragments arranged by size:
1877 1028 758 577 496 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 1424 890
Size: 534 4685
BsiEI CG_ry'CG
Cuts at: 104 2761 2807 4015 104
Size: 2657 46 1208 1308
Fragments arranged by size:
2657 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 959 1493 2233 3011 3201 3993 959
Size: 534 740 778 190 792 2185
Fragments arranged by size:
2185 792 778 740 534 190
Bsp1286I G_dGCh'C
Cuts at: 386 959 1493 2233 2844 2937 3011 3201 3263
Size: 573 534 740 611 93 74 190 62
Cuts at: 3263 3993 386
Size: 730 1612
Fragments arranged by size:
1612 740 730 611 573 534 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4439 4439
Size: 5219
BspHI T'CATG_A
Cuts at: 2180 4395 2180
Size: 2215 3004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 912 1446 1543 2269 2483 2785 3166 912
Size: 534 97 726 214 302 381 2965
Fragments arranged by size:
2965 726 534 381 302 214 97
BsrBI CCG'CTC
Cuts at: 50 3511 3565 5049 5214 50
Size: 3461 54 1484 165 55
Fragments arranged by size:
3461 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3131 3131
Size: 5219
BsrFI r'CCGG_y
Cuts at: 3217 3398 3607 3217
Size: 181 209 4829
Fragments arranged by size:
4829 209 181
BssSI C'ACGA_G
Cuts at: 3490 3848 3490
Size: 358 4861
BstAPI GCAn_nnn'nTGC
Cuts at: 2596 2596
Size: 5219
BstBI TT'CG_AA
Cuts at: 3580 3580
Size: 5219
BstXI CCAn_nnnn'nTGG
Cuts at: 576 1110 1644 576
Size: 534 534 4151
Fragments arranged by size:
4151 534 534
BstYI r'GATC_y
Cuts at: 1 489 991 2244 2711 2734 3069 3315 4327
Size: 488 502 1253 467 23 335 246 1012
Cuts at: 4327 4413 4425 4771 1
Size: 86 12 346 449
Fragments arranged by size:
1253 1012 502 488 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 528 528
Size: 5219
Bsu36I CC'TnA_GG
Cuts at: 1682 1682
Size: 5219
BtgI C'CryG_G
Cuts at: 107 575 1109 1643 107
Size: 468 534 534 3683
Fragments arranged by size:
3683 534 534 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3169 3350 3513 3169
Size: 181 163 4875
Fragments arranged by size:
4875 181 163
BtsI GCAGTG_nn'
Cuts at: 203 789 1857 2462 203
Size: 586 1068 605 2960
Fragments arranged by size:
2960 1068 605 586
DraI TTT'AAA
Cuts at: 2218 2218
Size: 5219
DrdI GACnn_nn'nnGTC
Cuts at: 2925 3783 2925
Size: 858 4361
EaeI y'GGCC_r
Cuts at: 513 1560 2094 2804 2978 3369 3396 4741 513
Size: 1047 534 710 174 391 27 1345 991
Fragments arranged by size:
1345 1047 991 710 534 391 174 27
EagI C'GGCC_G
Cuts at: 2804 2804
Size: 5219
EarI CTCTTCn'nnn_
Cuts at: 3242 3452 3242
Size: 210 5009
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3427 3737 3883 5029 3427
Size: 310 146 1146 3617
Fragments arranged by size:
3617 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 1491 2231 957
Size: 534 740 3945
Fragments arranged by size:
3945 740 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1608 3044 3476 4223 4491 540
Size: 1068 1436 432 747 268 1268
Fragments arranged by size:
1436 1268 1068 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 153 153
Size: 5219
EcoO109I rG'GnC_Cy
Cuts at: 382 701 1235 1273 1769 2060 2358 382
Size: 319 534 38 496 291 298 3243
Fragments arranged by size:
3243 534 496 319 298 291 38
EcoRI G'AATT_C
Cuts at: 2223 2223
Size: 5219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 5219
FspI TGC'GCA
Cuts at: 3000 3000
Size: 5219
HaeII r_GCGC'y
Cuts at: 2653 2901 3923 4902 2653
Size: 248 1022 979 2970
Fragments arranged by size:
2970 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 693 725 1227 1259 1761 1793 3061
Size: 32 533 32 502 32 502 32 1268
Cuts at: 3061 3091 3093 3123 128
Size: 30 2 30 2224
Fragments arranged by size:
2224 1268 533 502 502 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1311 2258 1311
Size: 947 4272
KasI G'GCGC_C
Cuts at: 2649 2897 2649
Size: 248 4971
KpnI G_GTAC'C
Cuts at: 2239 2239
Size: 5219
MluI A'CGCG_T
Cuts at: 4652 4652
Size: 5219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 1526 3890 4074 1127
Size: 399 2364 184 2272
Fragments arranged by size:
2364 2272 399 184
MscI TGG'CCA
Cuts at: 515 1562 2980 515
Size: 1047 1418 2754
Fragments arranged by size:
2754 1418 1047
MslI CAynn'nnrTG
Cuts at: 976 3208 3335 976
Size: 2232 127 2860
Fragments arranged by size:
2860 2232 127
MspA1I CmG'CkG
Cuts at: 305 684 1218 1523 1752 2161 2330 2644 3004
Size: 379 534 305 229 409 169 314 360
Cuts at: 3004 4017 4262 305
Size: 1013 245 1262
Fragments arranged by size:
1262 1013 534 409 379 360 314 305
245 229 169
NaeI GCC'GGC
Cuts at: 3400 3400
Size: 5219
NarI GG'CG_CC
Cuts at: 2650 2898 2650
Size: 248 4971
NcoI C'CATG_G
Cuts at: 107 107
Size: 5219
NgoMIV G'CCGG_C
Cuts at: 3398 3398
Size: 5219
NsiI A_TGCA'T
Cuts at: 423 423
Size: 5219
NspI r_CATG'y
Cuts at: 2272 2772 3679 2272
Size: 500 907 3812
Fragments arranged by size:
3812 907 500
PasI CC'CwG_GG
Cuts at: 379 379
Size: 5219
PciI A'CATG_T
Cuts at: 3675 3675
Size: 5219
PmeI GTTT'AAAC
Cuts at: 2218 2218
Size: 5219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 4358
Size: 32 5187
PpuMI rG'GwC_Cy
Cuts at: 701 1235 1273 1769 2060 701
Size: 534 38 496 291 3860
Fragments arranged by size:
3860 534 496 291 38
PshAI GACnn'nnGTC
Cuts at: 1042 1576 1042
Size: 534 4685
PspOMI G'GGCC_C
Cuts at: 382 382
Size: 5219
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1150 1182 1684 1716 1150
Size: 32 502 32 4653
Fragments arranged by size:
4653 502 32 32
PstI C_TGCA'G
Cuts at: 683 1217 1751 2266 2951 683
Size: 534 534 515 685 2951
Fragments arranged by size:
2951 685 534 534 515
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5219
PvuII CAG'CTG
Cuts at: 305 684 1218 1752 2161 2644 3004 305
Size: 379 534 534 409 483 360 2520
Fragments arranged by size:
2520 534 534 483 409 379 360
RsrII CG'GwC_CG
Cuts at: 3414 3414
Size: 5219
SacI G_AGCT'C
Cuts at: 959 1493 2233 959
Size: 534 740 3945
Fragments arranged by size:
3945 740 534
SalI G'TCGA_C
Cuts at: 2256 2256
Size: 5219
SapI GCTCTTCn'nnn_
Cuts at: 3242 3452 3242
Size: 210 5009
SbfI CC_TGCA'GG
Cuts at: 2266 2266
Size: 5219
ScaI AGT'ACT
Cuts at: 913 4736 913
Size: 3823 1396
SfcI C'TryA_G
Cuts at: 33 300 679 1127 1213 1574 1747 2262 2947
Size: 267 379 448 86 361 173 515 685
Cuts at: 2947 3940 4131 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 515 448 379 361 267
191 173 86
SfoI GGC'GCC
Cuts at: 2651 2899 2651
Size: 248 4971
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5219
SmaI CCC'GGG
Cuts at: 2241 2241
Size: 5219
SmlI C'TyrA_G
Cuts at: 2374 3289 3781 4043 2374
Size: 915 492 262 3550
Fragments arranged by size:
3550 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5219
SphI G_CATG'C
Cuts at: 2272 2772 2272
Size: 500 4719
StyI C'CwwG_G
Cuts at: 107 2353 2707 107
Size: 2246 354 2619
Fragments arranged by size:
2619 2246 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 964 1089 2802 2935 3394 3603 964
Size: 125 1713 133 459 209 2580
Fragments arranged by size:
2580 1713 459 209 133 125
TatI w'GTAC_w
Cuts at: 911 4734 911
Size: 3823 1396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4736 4768 4736
Size: 32 5187
Tth111I GACn'n_nGTC
Cuts at: 1324 1858 3016 1324
Size: 534 1158 3527
Fragments arranged by size:
3527 1158 534
XbaI T'CTAG_A
Cuts at: 2250 2250
Size: 5219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 292 2714 292
Size: 2422 2797
XmaI C'CCGG_G
Cuts at: 2239 2239
Size: 5219
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbsI BcgI BglI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PfoI PmlI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI