Restriction map for
pF1KE5061
(Circular) (Six-base) MAPSORT of: pF1KE5061.seq Check: 6453 from: 1 to: 4286
pF1KE5061 4286 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 839 2111 2543 3290 3558 820
Size: 19 1272 432 747 268 1548
Fragments arranged by size:
1548 1272 747 432 268 19
AflIII A'CryG_T
Cuts at: 137 1638 2742 3719 137
Size: 1501 1104 977 704
Fragments arranged by size:
1501 1104 977 704
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 1591 3158 1591
Size: 1567 2719
ApaLI G'TGCA_C
Cuts at: 3056 3056
Size: 4286
ApoI r'AATT_y
Cuts at: 262 439 882 1290 3639 3895 262
Size: 177 443 408 2349 256 653
Fragments arranged by size:
2349 653 443 408 256 177
AseI AT'TA_AT
Cuts at: 20 482 20
Size: 462 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 1306 1306
Size: 4286
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 1302 1716 1964 1999 1302
Size: 414 248 35 3589
Fragments arranged by size:
3589 414 248 35
BanII G_rGCy'C
Cuts at: 1300 2330 1300
Size: 1030 3256
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 808 808
Size: 4286
BbvCI CC'TCA_GC
Cuts at: 495 495
Size: 4286
BciVI GTATCCnnnnn_n'
Cuts at: 1104 2184 2945 4286 1104
Size: 1080 761 1341 1104
Fragments arranged by size:
1341 1104 1080 761
BclI T'GATC_A
Cuts at: 143 704 924 1806 143
Size: 561 220 882 2623
Fragments arranged by size:
2623 882 561 220
BglII A'GATC_T
Cuts at: 650 1801 3838 650
Size: 1151 2037 1098
Fragments arranged by size:
2037 1151 1098
BlpI GC'TnA_GC
Cuts at: 1398 1398
Size: 4286
Bme1580I G_kGCm'C
Cuts at: 1911 2004 3060 1911
Size: 93 1056 3137
Fragments arranged by size:
3137 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1498 1579 1914 4058 1498
Size: 81 335 2144 1726
Fragments arranged by size:
2144 1726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 639 952 535
Size: 104 313 3869
Fragments arranged by size:
3869 313 104
Bpu10I CC'TnA_GC
Cuts at: 495 495
Size: 4286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 506 1462 2341 2833 3131 316
Size: 190 956 879 492 298 1471
Fragments arranged by size:
1471 956 879 492 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 400 820 952 1153 3990 400
Size: 420 132 201 2837 696
Fragments arranged by size:
2837 696 420 201 132
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 850 1800 3837 6
Size: 844 950 2037 455
Fragments arranged by size:
2037 950 844 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1475 1996 2674 2948 3095 3506 1037
Size: 438 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 438 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 798 828 268
Size: 30 500 30 3726
Fragments arranged by size:
3726 500 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1072 1072
Size: 4286
BseYI C'CCAG_C
Cuts at: 871 1711 2469 3046 3542 871
Size: 840 758 577 496 1615
Fragments arranged by size:
1615 840 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3082 104
Size: 1724 46 1208 1308
Fragments arranged by size:
1724 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1300 2078 2268 3060 1300
Size: 778 190 792 2526
Fragments arranged by size:
2526 792 778 190
BsmI GAATG_Cn'
Cuts at: 117 210 117
Size: 93 4193
Bsp1286I G_dGCh'C
Cuts at: 1300 1911 2004 2078 2268 2330 3060 1300
Size: 611 93 74 190 62 730 2526
Fragments arranged by size:
2526 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1037 3506 1037
Size: 2469 1817
BspHI T'CATG_A
Cuts at: 3462 3462
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1336 1550 1852 2233 1036
Size: 300 214 302 381 3089
Fragments arranged by size:
3089 381 302 300 214
BsrBI CCG'CTC
Cuts at: 50 2578 2632 4116 4281 50
Size: 2528 54 1484 165 55
Fragments arranged by size:
2528 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 276 2198 276
Size: 1922 2364
BsrFI r'CCGG_y
Cuts at: 2284 2465 2674 2284
Size: 181 209 3896
Fragments arranged by size:
3896 209 181
BssSI C'ACGA_G
Cuts at: 2557 2915 2557
Size: 358 3928
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1663
Size: 4286
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 837 837
Size: 4286
BstYI r'GATC_y
Cuts at: 1 650 1311 1778 1801 2136 2382 3394 3480
Size: 649 661 467 23 335 246 1012 86
Cuts at: 3480 3492 3838 1
Size: 12 346 449
Fragments arranged by size:
1012 661 649 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 569 707 1529 569
Size: 138 822 3326
Fragments arranged by size:
3326 822 138
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 2850 1992
Size: 858 3428
EaeI y'GGCC_r
Cuts at: 1871 2045 2436 2463 3808 1871
Size: 174 391 27 1345 2349
Fragments arranged by size:
2349 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 754 1210 2309 2519 754
Size: 456 1099 210 2521
Fragments arranged by size:
2521 1099 456 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2494 2804 2950 4096 2494
Size: 310 146 1146 2684
Fragments arranged by size:
2684 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 639 820 839 952 2111 2543 3290 3558
Size: 104 181 19 113 1159 432 747 268
Cuts at: 3558 535
Size: 1263
Fragments arranged by size:
1263 1159 747 432 268 181 113 104
19
EcoO109I rG'GnC_Cy
Cuts at: 1425 1425
Size: 4286
EcoRI G'AATT_C
Cuts at: 439 1290 439
Size: 851 3435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 4286
FspI TGC'GCA
Cuts at: 2067 2067
Size: 4286
HaeII r_GCGC'y
Cuts at: 1720 1968 2990 3969 1720
Size: 248 1022 979 2037
Fragments arranged by size:
2037 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 599 631 2128 2158 2160 2190 268
Size: 32 299 32 1497 30 2 30 2364
Fragments arranged by size:
2364 1497 299 32 32 30 30 2
HincII GTy'rAC
Cuts at: 155 867 1325 155
Size: 712 458 3116
Fragments arranged by size:
3116 712 458
HindIII A'AGCT_T
Cuts at: 724 793 724
Size: 69 4217
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 103 2957 3141 103
Size: 2854 184 1248
Fragments arranged by size:
2854 1248 184
MscI TGG'CCA
Cuts at: 2047 2047
Size: 4286
MslI CAynn'nnrTG
Cuts at: 785 1276 2275 2402 785
Size: 491 999 127 2669
Fragments arranged by size:
2669 999 491 127
MspA1I CmG'CkG
Cuts at: 955 1397 1711 2071 3084 3329 955
Size: 442 314 360 1013 245 1912
Fragments arranged by size:
1912 1013 442 360 314 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 1339 1839 2746 1339
Size: 500 907 2879
Fragments arranged by size:
2879 907 500
PciI A'CATG_T
Cuts at: 2742 2742
Size: 4286
PfoI T'CCnGG_A
Cuts at: 434 434
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4254
PshAI GACnn'nnGTC
Cuts at: 808 808
Size: 4286
PsiI TTA'TAA
Cuts at: 613 1187 613
Size: 574 3712
PstI C_TGCA'G
Cuts at: 1333 2018 1333
Size: 685 3601
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 1711 2071 1711
Size: 360 3926
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 754 2309 2519 754
Size: 1555 210 2521
Fragments arranged by size:
2521 1555 210
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 1329 2014 3007 3198 33
Size: 1296 685 993 191 1121
Fragments arranged by size:
1296 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 331 521 1441 2356 2848 3110 331
Size: 190 920 915 492 262 1507
Fragments arranged by size:
1507 920 915 492 262 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
SspI AAT'ATT
Cuts at: 480 480
Size: 4286
StyI C'CwwG_G
Cuts at: 1420 1774 1420
Size: 354 3932
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1869 2002 2461 2670 1869
Size: 133 459 209 3485
Fragments arranged by size:
3485 459 209 133
TatI w'GTAC_w
Cuts at: 3801 3801
Size: 4286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 798 830 3803 3835 798
Size: 32 2973 32 1249
Fragments arranged by size:
2973 1249 32 32
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1781 1781
Size: 4286
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI