Restriction map for
pF1KE5139
(Circular) (Six-base) MAPSORT of: pF1KE5139.seq Check: 3725 from: 1 to: 3458
pF1KE5139 3458 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 496 496
Size: 3458
Acc65I G'GTAC_C
Cuts at: 474 474
Size: 3458
AclI AA'CG_TT
Cuts at: 3298 3298
Size: 3458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 1715 2462 2730 1283
Size: 432 747 268 2011
Fragments arranged by size:
2011 747 432 268
AflIII A'CryG_T
Cuts at: 450 810 1914 2891 450
Size: 360 1104 977 1017
Fragments arranged by size:
1104 1017 977 360
AgeI A'CCGG_T
Cuts at: 1846 1846
Size: 3458
AlwNI CAG_nnn'CTG
Cuts at: 763 2330 763
Size: 1567 1891
ApaLI G'TGCA_C
Cuts at: 2228 2228
Size: 3458
ApoI r'AATT_y
Cuts at: 462 2811 3067 462
Size: 2349 256 853
Fragments arranged by size:
2349 853 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3458
AvaI C'yCGr_G
Cuts at: 478 478
Size: 3458
BamHI G'GATC_C
Cuts at: 483 483
Size: 3458
BanI G'GyrC_C
Cuts at: 474 888 1136 1171 474
Size: 414 248 35 2761
Fragments arranged by size:
2761 414 248 35
BanII G_rGCy'C
Cuts at: 131 237 472 1502 131
Size: 106 235 1030 2087
Fragments arranged by size:
2087 1030 235 106
BbeI G_GCGC'C
Cuts at: 892 1140 892
Size: 248 3210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3424
BciVI GTATCCnnnnn_n'
Cuts at: 1356 2117 3458 1356
Size: 761 1341 1356
Fragments arranged by size:
1356 1341 761
BclI T'GATC_A
Cuts at: 978 978
Size: 3458
BglII A'GATC_T
Cuts at: 973 3010 973
Size: 2037 1421
BlpI GC'TnA_GC
Cuts at: 570 570
Size: 3458
Bme1580I G_kGCm'C
Cuts at: 432 1083 1176 2232 432
Size: 651 93 1056 1658
Fragments arranged by size:
1658 1056 651 93
BmrI ACTGGGnnnn_n'
Cuts at: 134 670 751 1086 3230 134
Size: 536 81 335 2144 362
Fragments arranged by size:
2144 536 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 249
Size: 3458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1513 2005 2303 634
Size: 879 492 298 1789
Fragments arranged by size:
1789 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3162 3162
Size: 3458
BsaAI yAC'GTr
Cuts at: 811 811
Size: 3458
BsaBI GATnn'nnATC
Cuts at: 6 972 3009 6
Size: 966 2037 455
Fragments arranged by size:
2037 966 455
BsaHI Gr'CG_yC
Cuts at: 889 1137 1839 3335 889
Size: 248 702 1496 1012
Fragments arranged by size:
1496 1012 702 248
BsaWI w'CCGG_w
Cuts at: 647 1168 1846 2120 2267 2678 647
Size: 521 678 274 147 411 1427
Fragments arranged by size:
1427 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 196 883 1641 2218 2714 196
Size: 687 758 577 496 940
Fragments arranged by size:
940 758 687 577 496
BsiEI CG_ry'CG
Cuts at: 104 1000 1046 2254 104
Size: 896 46 1208 1308
Fragments arranged by size:
1308 1208 896 46
BsiHKAI G_wGCw'C
Cuts at: 237 472 1250 1440 2232 237
Size: 235 778 190 792 1463
Fragments arranged by size:
1463 792 778 235 190
BsmBI CGTCTCn'nnnn_
Cuts at: 406 406
Size: 3458
Bsp1286I G_dGCh'C
Cuts at: 131 237 432 472 1083 1176 1250 1440 1502
Size: 106 195 40 611 93 74 190 62
Cuts at: 1502 2232 131
Size: 730 1357
Fragments arranged by size:
1357 730 611 195 190 106 93 74
62 40
BspEI T'CCGG_A
Cuts at: 2678 2678
Size: 3458
BspHI T'CATG_A
Cuts at: 441 2634 441
Size: 2193 1265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 508 722 1024 1405 508
Size: 214 302 381 2561
Fragments arranged by size:
2561 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1750 1804 3288 3453 50
Size: 1700 54 1484 165 55
Fragments arranged by size:
1700 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1370 1370
Size: 3458
BsrFI r'CCGG_y
Cuts at: 1456 1637 1846 1456
Size: 181 209 3068
Fragments arranged by size:
3068 209 181
BssSI C'ACGA_G
Cuts at: 1729 2087 1729
Size: 358 3100
BstAPI GCAn_nnn'nTGC
Cuts at: 835 835
Size: 3458
BstBI TT'CG_AA
Cuts at: 1819 1819
Size: 3458
BstYI r'GATC_y
Cuts at: 1 483 950 973 1308 1554 2566 2652 2664
Size: 482 467 23 335 246 1012 86 12
Cuts at: 2664 3010 1
Size: 346 449
Fragments arranged by size:
1012 482 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1408 1589 1752 1408
Size: 181 163 3114
Fragments arranged by size:
3114 181 163
BtsI GCAGTG_nn'
Cuts at: 701 701
Size: 3458
DraI TTT'AAA
Cuts at: 457 457
Size: 3458
DrdI GACnn_nn'nnGTC
Cuts at: 1164 2022 1164
Size: 858 2600
EaeI y'GGCC_r
Cuts at: 1043 1217 1608 1635 2980 1043
Size: 174 391 27 1345 1521
Fragments arranged by size:
1521 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1043 1043
Size: 3458
EarI CTCTTCn'nnn_
Cuts at: 119 239 1481 1691 119
Size: 120 1242 210 1886
Fragments arranged by size:
1886 1242 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1666 1976 2122 3268 1666
Size: 310 146 1146 1856
Fragments arranged by size:
1856 1146 310 146
EcoICRI GAG'CTC
Cuts at: 235 470 235
Size: 235 3223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 1283 1715 2462 2730 249
Size: 1034 432 747 268 977
Fragments arranged by size:
1034 977 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 597 597
Size: 3458
EcoRI G'AATT_C
Cuts at: 462 462
Size: 3458
EcoRV GAT'ATC
Cuts at: 399 399
Size: 3458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1477 1477
Size: 3458
FspI TGC'GCA
Cuts at: 1239 1239
Size: 3458
HaeII r_GCGC'y
Cuts at: 892 1140 2162 3141 892
Size: 248 1022 979 1209
Fragments arranged by size:
1209 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1300 1330 1332 1362 1300
Size: 30 2 30 3396
Fragments arranged by size:
3396 30 30 2
HincII GTy'rAC
Cuts at: 497 497
Size: 3458
HindIII A'AGCT_T
Cuts at: 296 296
Size: 3458
KasI G'GCGC_C
Cuts at: 888 1136 888
Size: 248 3210
KpnI G_GTAC'C
Cuts at: 478 478
Size: 3458
MluI A'CGCG_T
Cuts at: 2891 2891
Size: 3458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2313 2129
Size: 184 3274
MscI TGG'CCA
Cuts at: 1219 1219
Size: 3458
MslI CAynn'nnrTG
Cuts at: 440 1447 1574 440
Size: 1007 127 2324
Fragments arranged by size:
2324 1007 127
MspA1I CmG'CkG
Cuts at: 569 883 1243 2256 2501 569
Size: 314 360 1013 245 1526
Fragments arranged by size:
1526 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1639 1639
Size: 3458
NarI GG'CG_CC
Cuts at: 889 1137 889
Size: 248 3210
NgoMIV G'CCGG_C
Cuts at: 1637 1637
Size: 3458
NspI r_CATG'y
Cuts at: 511 1011 1918 511
Size: 500 907 2051
Fragments arranged by size:
2051 907 500
PciI A'CATG_T
Cuts at: 1914 1914
Size: 3458
PmeI GTTT'AAAC
Cuts at: 457 457
Size: 3458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 366 398 2597 2629 366
Size: 32 2199 32 1195
Fragments arranged by size:
2199 1195 32 32
PstI C_TGCA'G
Cuts at: 505 1190 505
Size: 685 2773
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3458
PvuII CAG'CTG
Cuts at: 883 1243 883
Size: 360 3098
RsrII CG'GwC_CG
Cuts at: 1653 1653
Size: 3458
SacI G_AGCT'C
Cuts at: 237 472 237
Size: 235 3223
SalI G'TCGA_C
Cuts at: 495 495
Size: 3458
SapI GCTCTTCn'nnn_
Cuts at: 119 1481 1691 119
Size: 1362 210 1886
Fragments arranged by size:
1886 1362 210
SbfI CC_TGCA'GG
Cuts at: 505 505
Size: 3458
ScaI AGT'ACT
Cuts at: 2975 2975
Size: 3458
SexAI A'CCwGG_T
Cuts at: 404 404
Size: 3458
SfcI C'TryA_G
Cuts at: 33 501 1186 2179 2370 33
Size: 468 685 993 191 1121
Fragments arranged by size:
1121 993 685 468 191
SfoI GGC'GCC
Cuts at: 890 1138 890
Size: 248 3210
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3458
SmaI CCC'GGG
Cuts at: 480 480
Size: 3458
SmlI C'TyrA_G
Cuts at: 613 1528 2020 2282 613
Size: 915 492 262 1789
Fragments arranged by size:
1789 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3458
SphI G_CATG'C
Cuts at: 511 1011 511
Size: 500 2958
StyI C'CwwG_G
Cuts at: 592 946 592
Size: 354 3104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1041 1174 1633 1842 1041
Size: 133 459 209 2657
Fragments arranged by size:
2657 459 209 133
TatI w'GTAC_w
Cuts at: 2973 2973
Size: 3458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3426
Tth111I GACn'n_nGTC
Cuts at: 1255 1255
Size: 3458
XbaI T'CTAG_A
Cuts at: 489 489
Size: 3458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 953 953
Size: 3458
XmaI C'CCGG_G
Cuts at: 478 478
Size: 3458
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI