Restriction map for
pF1KE5152
(Circular) (Six-base) MAPSORT of: pF1KE5152.seq Check: 6426 from: 1 to: 3374
pF1KE5152 3374 bp
With 182 enzymes: SgfI *
October 31, 2014 16:16 ..
AccI GT'mk_AC
Cuts at: 412 412
Size: 3374
Acc65I G'GTAC_C
Cuts at: 390 390
Size: 3374
AclI AA'CG_TT
Cuts at: 3214 3214
Size: 3374
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1631 2378 2646 1199
Size: 432 747 268 1927
Fragments arranged by size:
1927 747 432 268
AflIII A'CryG_T
Cuts at: 726 1830 2807 726
Size: 1104 977 1293
Fragments arranged by size:
1293 1104 977
AgeI A'CCGG_T
Cuts at: 1762 1762
Size: 3374
AlwNI CAG_nnn'CTG
Cuts at: 679 2246 679
Size: 1567 1807
ApaLI G'TGCA_C
Cuts at: 2144 2144
Size: 3374
ApoI r'AATT_y
Cuts at: 378 2727 2983 378
Size: 2349 256 769
Fragments arranged by size:
2349 769 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3374
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3374
AvaI C'yCGr_G
Cuts at: 394 394
Size: 3374
BamHI G'GATC_C
Cuts at: 399 399
Size: 3374
BanI G'GyrC_C
Cuts at: 390 804 1052 1087 390
Size: 414 248 35 2677
Fragments arranged by size:
2677 414 248 35
BanII G_rGCy'C
Cuts at: 233 388 1418 233
Size: 155 1030 2189
Fragments arranged by size:
2189 1030 155
BbeI G_GCGC'C
Cuts at: 808 1056 808
Size: 248 3126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 252 286 90
Size: 34 128 34 3178
Fragments arranged by size:
3178 128 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1272 2033 3374 1272
Size: 761 1341 1272
Fragments arranged by size:
1341 1272 761
BclI T'GATC_A
Cuts at: 894 894
Size: 3374
BglII A'GATC_T
Cuts at: 889 2926 889
Size: 2037 1337
BlpI GC'TnA_GC
Cuts at: 357 486 357
Size: 129 3245
Bme1580I G_kGCm'C
Cuts at: 999 1092 2148 999
Size: 93 1056 2225
Fragments arranged by size:
2225 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 586 667 1002 3146 586
Size: 81 335 2144 814
Fragments arranged by size:
2144 814 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 3374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1429 1921 2219 550
Size: 879 492 298 1705
Fragments arranged by size:
1705 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3078 3078
Size: 3374
BsaAI yAC'GTr
Cuts at: 727 727
Size: 3374
BsaBI GATnn'nnATC
Cuts at: 6 888 2925 6
Size: 882 2037 455
Fragments arranged by size:
2037 882 455
BsaHI Gr'CG_yC
Cuts at: 805 1053 1755 3251 805
Size: 248 702 1496 928
Fragments arranged by size:
1496 928 702 248
BsaWI w'CCGG_w
Cuts at: 313 563 1084 1762 2036 2183 2594 313
Size: 250 521 678 274 147 411 1093
Fragments arranged by size:
1093 678 521 411 274 250 147
BseYI C'CCAG_C
Cuts at: 241 799 1557 2134 2630 241
Size: 558 758 577 496 985
Fragments arranged by size:
985 758 577 558 496
BsiEI CG_ry'CG
Cuts at: 104 916 962 2170 104
Size: 812 46 1208 1308
Fragments arranged by size:
1308 1208 812 46
BsiHKAI G_wGCw'C
Cuts at: 388 1166 1356 2148 388
Size: 778 190 792 1614
Fragments arranged by size:
1614 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 233 388 999 1092 1166 1356 1418 2148 233
Size: 155 611 93 74 190 62 730 1459
Fragments arranged by size:
1459 730 611 190 155 93 74 62
BspEI T'CCGG_A
Cuts at: 2594 2594
Size: 3374
BspHI T'CATG_A
Cuts at: 275 2550 275
Size: 2275 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 424 638 940 1321 424
Size: 214 302 381 2477
Fragments arranged by size:
2477 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1666 1720 3204 3369 50
Size: 1616 54 1484 165 55
Fragments arranged by size:
1616 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1286 1286
Size: 3374
BsrFI r'CCGG_y
Cuts at: 1372 1553 1762 1372
Size: 181 209 2984
Fragments arranged by size:
2984 209 181
BssSI C'ACGA_G
Cuts at: 1645 2003 1645
Size: 358 3016
BstAPI GCAn_nnn'nTGC
Cuts at: 751 751
Size: 3374
BstBI TT'CG_AA
Cuts at: 1735 1735
Size: 3374
BstEII G'GTnAC_C
Cuts at: 117 316 117
Size: 199 3175
BstYI r'GATC_y
Cuts at: 1 399 866 889 1224 1470 2482 2568 2580
Size: 398 467 23 335 246 1012 86 12
Cuts at: 2580 2926 1
Size: 346 449
Fragments arranged by size:
1012 467 449 398 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 257 257
Size: 3374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1324 1505 1668 1324
Size: 181 163 3030
Fragments arranged by size:
3030 181 163
BtsI GCAGTG_nn'
Cuts at: 617 617
Size: 3374
DraI TTT'AAA
Cuts at: 373 373
Size: 3374
DrdI GACnn_nn'nnGTC
Cuts at: 1080 1938 1080
Size: 858 2516
EaeI y'GGCC_r
Cuts at: 303 959 1133 1524 1551 2896 303
Size: 656 174 391 27 1345 781
Fragments arranged by size:
1345 781 656 391 174 27
EagI C'GGCC_G
Cuts at: 959 959
Size: 3374
EarI CTCTTCn'nnn_
Cuts at: 1397 1607 1397
Size: 210 3164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1582 1892 2038 3184 1582
Size: 310 146 1146 1772
Fragments arranged by size:
1772 1146 310 146
EcoICRI GAG'CTC
Cuts at: 386 386
Size: 3374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1199 1631 2378 2646 202
Size: 997 432 747 268 930
Fragments arranged by size:
997 930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 513 513
Size: 3374
EcoRI G'AATT_C
Cuts at: 378 378
Size: 3374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1393 1393
Size: 3374
FspI TGC'GCA
Cuts at: 1155 1155
Size: 3374
HaeII r_GCGC'y
Cuts at: 808 1056 2078 3057 808
Size: 248 1022 979 1125
Fragments arranged by size:
1125 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 324 356 1216 1246 1248 1278 324
Size: 32 860 30 2 30 2420
Fragments arranged by size:
2420 860 32 30 30 2
HincII GTy'rAC
Cuts at: 413 413
Size: 3374
KasI G'GCGC_C
Cuts at: 804 1052 804
Size: 248 3126
KpnI G_GTAC'C
Cuts at: 394 394
Size: 3374
MluI A'CGCG_T
Cuts at: 2807 2807
Size: 3374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2045 2229 2045
Size: 184 3190
MscI TGG'CCA
Cuts at: 305 1135 305
Size: 830 2544
MslI CAynn'nnrTG
Cuts at: 274 1363 1490 274
Size: 1089 127 2158
Fragments arranged by size:
2158 1089 127
MspA1I CmG'CkG
Cuts at: 485 799 1159 2172 2417 485
Size: 314 360 1013 245 1442
Fragments arranged by size:
1442 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1555 1555
Size: 3374
NarI GG'CG_CC
Cuts at: 805 1053 805
Size: 248 3126
NcoI C'CATG_G
Cuts at: 257 257
Size: 3374
NgoMIV G'CCGG_C
Cuts at: 1553 1553
Size: 3374
NspI r_CATG'y
Cuts at: 427 927 1834 427
Size: 500 907 1967
Fragments arranged by size:
1967 907 500
PasI CC'CwG_GG
Cuts at: 123 123
Size: 3374
PciI A'CATG_T
Cuts at: 1830 1830
Size: 3374
PfoI T'CCnGG_A
Cuts at: 158 158
Size: 3374
PmeI GTTT'AAAC
Cuts at: 373 373
Size: 3374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2513 2545 2513
Size: 32 3342
PstI C_TGCA'G
Cuts at: 421 1106 421
Size: 685 2689
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3374
PvuII CAG'CTG
Cuts at: 799 1159 799
Size: 360 3014
RsrII CG'GwC_CG
Cuts at: 1569 1569
Size: 3374
SacI G_AGCT'C
Cuts at: 388 388
Size: 3374
SalI G'TCGA_C
Cuts at: 411 411
Size: 3374
SapI GCTCTTCn'nnn_
Cuts at: 1397 1607 1397
Size: 210 3164
SbfI CC_TGCA'GG
Cuts at: 421 421
Size: 3374
ScaI AGT'ACT
Cuts at: 2891 2891
Size: 3374
SfcI C'TryA_G
Cuts at: 33 417 1102 2095 2286 33
Size: 384 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 191
SfoI GGC'GCC
Cuts at: 806 1054 806
Size: 248 3126
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3374
SmaI CCC'GGG
Cuts at: 396 396
Size: 3374
SmlI C'TyrA_G
Cuts at: 529 1444 1936 2198 529
Size: 915 492 262 1705
Fragments arranged by size:
1705 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3374
SphI G_CATG'C
Cuts at: 427 927 427
Size: 500 2874
StyI C'CwwG_G
Cuts at: 257 508 862 257
Size: 251 354 2769
Fragments arranged by size:
2769 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 957 1090 1549 1758 957
Size: 133 459 209 2573
Fragments arranged by size:
2573 459 209 133
TatI w'GTAC_w
Cuts at: 2889 2889
Size: 3374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3342
Tth111I GACn'n_nGTC
Cuts at: 1171 1171
Size: 3374
XbaI T'CTAG_A
Cuts at: 405 405
Size: 3374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 869 869
Size: 3374
XmaI C'CCGG_G
Cuts at: 394 394
Size: 3374
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI