Restriction map for
pF1KE5297
(Circular) (Six-base) MAPSORT of: pF1KE5297.seq Check: 5832 from: 1 to: 6524
pF1KE5297 6524 bp
With 182 enzymes: SgfI *
December 2, 2014 13:35 ..
AccI GT'mk_AC
Cuts at: 779 3562 779
Size: 2783 3741
Acc65I G'GTAC_C
Cuts at: 2510 3540 2510
Size: 1030 5494
AclI AA'CG_TT
Cuts at: 835 6364 835
Size: 5529 995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 675 809 858 1279 1635 4349 4781 5528
Size: 212 134 49 421 356 2714 432 747
Cuts at: 5528 5796 463
Size: 268 1191
Fragments arranged by size:
2714 1191 747 432 421 356 268 212
134 49
AfeI AGC'GCT
Cuts at: 2333 2333
Size: 6524
AflIII A'CryG_T
Cuts at: 743 782 2099 2345 2364 3876 4980 5957 743
Size: 39 1317 246 19 1512 1104 977 1310
Fragments arranged by size:
1512 1317 1310 1104 977 246 39 19
AgeI A'CCGG_T
Cuts at: 4912 4912
Size: 6524
AleI CACnn'nnGTG
Cuts at: 742 1633 2104 2617 2951 742
Size: 891 471 513 334 4315
Fragments arranged by size:
4315 891 513 471 334
AlwNI CAG_nnn'CTG
Cuts at: 206 537 1257 1617 2967 3021 3829 5396 206
Size: 331 720 360 1350 54 808 1567 1334
Fragments arranged by size:
1567 1350 1334 808 720 360 331 54
ApaI G_GGCC'C
Cuts at: 3212 3212
Size: 6524
ApaLI G'TGCA_C
Cuts at: 1759 2742 5294 1759
Size: 983 2552 2989
Fragments arranged by size:
2989 2552 983
ApoI r'AATT_y
Cuts at: 574 2683 2698 3528 5877 6133 574
Size: 2109 15 830 2349 256 965
Fragments arranged by size:
2349 2109 965 830 256 15
AseI AT'TA_AT
Cuts at: 20 1088 20
Size: 1068 5456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
AvaI C'yCGr_G
Cuts at: 2066 2393 2822 2937 3544 2066
Size: 327 429 115 607 5046
Fragments arranged by size:
5046 607 429 327 115
BamHI G'GATC_C
Cuts at: 1142 3549 1142
Size: 2407 4117
BanI G'GyrC_C
Cuts at: 286 658 1324 1737 2389 2510 3439 3540 3954
Size: 372 666 413 652 121 929 101 414
Cuts at: 3954 4202 4237 286
Size: 248 35 2573
Fragments arranged by size:
2573 929 666 652 414 413 372 248
121 101 35
BanII G_rGCy'C
Cuts at: 1705 2431 3058 3212 3538 4568 1705
Size: 726 627 154 326 1030 3661
Fragments arranged by size:
3661 1030 726 627 326 154
BbeI G_GCGC'C
Cuts at: 662 1328 1741 3443 3958 4206 662
Size: 666 413 1702 515 248 2980
Fragments arranged by size:
2980 1702 666 515 413 248
BbsI GAAGACnn'nnnn_
Cuts at: 259 798 1641 1904 2541 3084 259
Size: 539 843 263 637 543 3699
Fragments arranged by size:
3699 843 637 543 539 263
BbvCI CC'TCA_GC
Cuts at: 1021 1597 3453 1021
Size: 576 1856 4092
Fragments arranged by size:
4092 1856 576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2403 2437 2403
Size: 34 6490
BciVI GTATCCnnnnn_n'
Cuts at: 1759 4422 5183 6524 1759
Size: 2663 761 1341 1759
Fragments arranged by size:
2663 1759 1341 761
BclI T'GATC_A
Cuts at: 617 1928 4044 617
Size: 1311 2116 3097
Fragments arranged by size:
3097 2116 1311
BglI GCCn_nnn'nGGC
Cuts at: 556 556
Size: 6524
BglII A'GATC_T
Cuts at: 1472 4039 6076 1472
Size: 2567 2037 1920
Fragments arranged by size:
2567 2037 1920
BlpI GC'TnA_GC
Cuts at: 1525 2203 2878 3636 1525
Size: 678 675 758 4413
Fragments arranged by size:
4413 758 678 675
Bme1580I G_kGCm'C
Cuts at: 1763 2392 2746 3212 4149 4242 5298 1763
Size: 629 354 466 937 93 1056 2989
Fragments arranged by size:
2989 1056 937 629 466 354 93
BmgBI CAC'GTC
Cuts at: 999 2102 999
Size: 1103 5421
BmrI ACTGGGnnnn_n'
Cuts at: 3736 3817 4152 6296 3736
Size: 81 335 2144 3964
Fragments arranged by size:
3964 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2457 4891 2457
Size: 2434 4090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 202 694 2275 2674 3058 180
Size: 22 492 1581 399 384 3646
Fragments arranged by size:
3646 1581 492 399 384 22
Bpu10I CC'TnA_GC
Cuts at: 1021 1597 3453 1021
Size: 576 1856 4092
Fragments arranged by size:
4092 1856 576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4579 5071 5369 3700
Size: 879 492 298 4855
Fragments arranged by size:
4855 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 821 2445 6228 821
Size: 1624 3783 1117
Fragments arranged by size:
3783 1624 1117
BsaAI yAC'GTr
Cuts at: 1521 2367 3877 1521
Size: 846 1510 4168
Fragments arranged by size:
4168 1510 846
BsaBI GATnn'nnATC
Cuts at: 6 4038 6075 6
Size: 4032 2037 455
Fragments arranged by size:
4032 2037 455
BsaHI Gr'CG_yC
Cuts at: 659 1325 1738 2666 3440 3955 4203 4905 6401
Size: 666 413 928 774 515 248 702 1496
Cuts at: 6401 659
Size: 782
Fragments arranged by size:
1496 928 782 774 702 666 515 413
248
BsaWI w'CCGG_w
Cuts at: 2195 3356 3713 4234 4912 5186 5333 5744 2195
Size: 1161 357 521 678 274 147 411 2975
Fragments arranged by size:
2975 1161 678 521 411 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 1786 1816 2272 2302 178
Size: 30 1578 30 456 30 4400
Fragments arranged by size:
4400 1578 456 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 568 1210 1585 2281 2368 3247 3250 3430
Size: 102 642 375 696 87 879 3 180
Cuts at: 3430 466
Size: 3560
Fragments arranged by size:
3560 879 696 642 375 180 102 87
3
BseYI C'CCAG_C
Cuts at: 190 654 2561 2870 2960 3949 4707 5284 5780
Size: 464 1907 309 90 989 758 577 496
Cuts at: 5780 190
Size: 934
Fragments arranged by size:
1907 989 934 758 577 496 464 309
90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 748 1501 2742 2975 3253 268
Size: 480 753 1241 233 278 3539
Fragments arranged by size:
3539 1241 753 480 278 233
BsiEI CG_ry'CG
Cuts at: 104 4066 4112 5320 104
Size: 3962 46 1208 1308
Fragments arranged by size:
3962 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1526 1763 2746 3538 4316 4506 5298 1526
Size: 237 983 792 778 190 792 2752
Fragments arranged by size:
2752 983 792 792 778 237 190
BsmI GAATG_Cn'
Cuts at: 804 1682 3426 804
Size: 878 1744 3902
Fragments arranged by size:
3902 1744 878
BsmBI CGTCTCn'nnnn_
Cuts at: 2821 2821
Size: 6524
Bsp1286I G_dGCh'C
Cuts at: 1526 1705 1763 2392 2431 2746 3058 3212 3538
Size: 179 58 629 39 315 312 154 326
Cuts at: 3538 4149 4242 4316 4506 4568 5298 1526
Size: 611 93 74 190 62 730 2752
Fragments arranged by size:
2752 730 629 611 326 315 312 190
179 154 93 74 62 58 39
BspEI T'CCGG_A
Cuts at: 5744 5744
Size: 6524
BspHI T'CATG_A
Cuts at: 5700 5700
Size: 6524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 707 1523 3574 3788 4090 4471 707
Size: 816 2051 214 302 381 2760
Fragments arranged by size:
2760 2051 816 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1004 4816 4870 6354 6519 50
Size: 954 3812 54 1484 165 55
Fragments arranged by size:
3812 1484 954 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2648 4436 2648
Size: 1788 4736
BsrFI r'CCGG_y
Cuts at: 702 1344 2152 3171 3380 4522 4703 4912 702
Size: 642 808 1019 209 1142 181 209 2314
Fragments arranged by size:
2314 1142 1019 808 642 209 209 181
BsrGI T'GTAC_A
Cuts at: 746 1826 746
Size: 1080 5444
BssSI C'ACGA_G
Cuts at: 4795 5153 4795
Size: 358 6166
BstAPI GCAn_nnn'nTGC
Cuts at: 2127 3901 2127
Size: 1774 4750
BstBI TT'CG_AA
Cuts at: 4885 4885
Size: 6524
BstEII G'GTnAC_C
Cuts at: 2625 2625
Size: 6524
BstXI CCAn_nnnn'nTGG
Cuts at: 1059 1059
Size: 6524
BstYI r'GATC_y
Cuts at: 1 878 1142 1253 1472 1875 2162 3549 4016
Size: 877 264 111 219 403 287 1387 467
Cuts at: 4016 4039 4374 4620 5632 5718 5730 6076 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1387 1012 877 467 449 403 346 335
287 264 246 219 111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2465 2465
Size: 6524
BtgI C'CryG_G
Cuts at: 107 230 942 1328 1535 1677 2073 2312 2594
Size: 123 712 386 207 142 396 239 282
Cuts at: 2594 2630 2660 107
Size: 36 30 3971
Fragments arranged by size:
3971 712 396 386 282 239 207 142
123 36 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4474 4655 4818 4474
Size: 181 163 6180
Fragments arranged by size:
6180 181 163
BtsI GCAGTG_nn'
Cuts at: 864 1621 2725 3767 864
Size: 757 1104 1042 3621
Fragments arranged by size:
3621 1104 1042 757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 212 247 212
Size: 35 6489
DraI TTT'AAA
Cuts at: 770 1137 3523 770
Size: 367 2386 3771
Fragments arranged by size:
3771 2386 367
DraIII CAC_nnn'GTG
Cuts at: 2370 2370
Size: 6524
DrdI GACnn_nn'nnGTC
Cuts at: 1378 2779 4230 5088 1378
Size: 1401 1451 858 2814
Fragments arranged by size:
2814 1451 1401 858
EaeI y'GGCC_r
Cuts at: 1366 1637 2150 3173 4109 4283 4674 4701 6046
Size: 271 513 1023 936 174 391 27 1345
Cuts at: 6046 1366
Size: 1844
Fragments arranged by size:
1844 1345 1023 936 513 391 271 174
27
EagI C'GGCC_G
Cuts at: 4109 4109
Size: 6524
EarI CTCTTCn'nnn_
Cuts at: 4547 4757 4547
Size: 210 6314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1171 1501 3367 4732 5042 5188 6334 1171
Size: 330 1866 1365 310 146 1146 1361
Fragments arranged by size:
1866 1365 1361 1146 330 310 146
EcoICRI GAG'CTC
Cuts at: 3536 3536
Size: 6524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 202 463 675 694 809 858 1279 1635
Size: 22 261 212 19 115 49 421 356
Cuts at: 1635 2275 2674 3058 4349 4781 5528 5796 180
Size: 640 399 384 1291 432 747 268 908
Fragments arranged by size:
1291 908 747 640 432 421 399 384
356 268 261 212 115 49 22 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1150 1150
Size: 6524
EcoO109I rG'GnC_Cy
Cuts at: 366 2244 2819 3123 3209 3663 366
Size: 1878 575 304 86 454 3227
Fragments arranged by size:
3227 1878 575 454 304 86
EcoRI G'AATT_C
Cuts at: 574 2683 3528 574
Size: 2109 845 3570
Fragments arranged by size:
3570 2109 845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 660 4543 660
Size: 3883 2641
FspI TGC'GCA
Cuts at: 4305 4305
Size: 6524
HaeII r_GCGC'y
Cuts at: 662 1328 1741 2335 3443 3958 4206 5228 6207
Size: 666 413 594 1108 515 248 1022 979
Cuts at: 6207 662
Size: 979
Fragments arranged by size:
1108 1022 979 979 666 594 515 413
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 2069 2101 4366 4396 4398 4428 418
Size: 32 1619 32 2265 30 2 30 2514
Fragments arranged by size:
2514 2265 1619 32 32 30 30 2
HincII GTy'rAC
Cuts at: 839 3563 839
Size: 2724 3800
HindIII A'AGCT_T
Cuts at: 1750 1750
Size: 6524
KasI G'GCGC_C
Cuts at: 658 1324 1737 3439 3954 4202 658
Size: 666 413 1702 515 248 2980
Fragments arranged by size:
2980 1702 666 515 413 248
KpnI G_GTAC'C
Cuts at: 2514 3544 2514
Size: 1030 5494
MluI A'CGCG_T
Cuts at: 5957 5957
Size: 6524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 5195 5379 2468
Size: 2727 184 3613
Fragments arranged by size:
3613 2727 184
MscI TGG'CCA
Cuts at: 1368 1639 4285 1368
Size: 271 2646 3607
Fragments arranged by size:
3607 2646 271
MslI CAynn'nnrTG
Cuts at: 742 1633 2104 2599 2617 2887 2951 4513 4640
Size: 891 471 495 18 270 64 1562 127
Cuts at: 4640 742
Size: 2626
Fragments arranged by size:
2626 1562 891 495 471 270 127 64
18
MspA1I CmG'CkG
Cuts at: 206 732 944 2505 2561 2617 2662 2874 3129
Size: 526 212 1561 56 56 45 212 255
Cuts at: 3129 3635 3949 4309 5322 5567 206
Size: 506 314 360 1013 245 1163
Fragments arranged by size:
1561 1163 1013 526 506 360 314 255
245 212 212 56 56 45
NaeI GCC'GGC
Cuts at: 3173 3382 4705 3173
Size: 209 1323 4992
Fragments arranged by size:
4992 1323 209
NarI GG'CG_CC
Cuts at: 659 1325 1738 3440 3955 4203 659
Size: 666 413 1702 515 248 2980
Fragments arranged by size:
2980 1702 666 515 413 248
NcoI C'CATG_G
Cuts at: 107 1535 1677 2312 2594 107
Size: 1428 142 635 282 4037
Fragments arranged by size:
4037 1428 635 282 142
NgoMIV G'CCGG_C
Cuts at: 3171 3380 4703 3171
Size: 209 1323 4992
Fragments arranged by size:
4992 1323 209
NspI r_CATG'y
Cuts at: 507 786 1448 2349 3577 4077 4984 507
Size: 279 662 901 1228 500 907 2047
Fragments arranged by size:
2047 1228 907 901 662 500 279
PasI CC'CwG_GG
Cuts at: 1068 1068
Size: 6524
PciI A'CATG_T
Cuts at: 782 2345 4980 782
Size: 1563 2635 2326
Fragments arranged by size:
2635 2326 1563
PflMI CCAn_nnn'nTGG
Cuts at: 537 2967 537
Size: 2430 4094
PfoI T'CCnGG_A
Cuts at: 671 2276 3179 671
Size: 1605 903 4016
Fragments arranged by size:
4016 1605 903
PmeI GTTT'AAAC
Cuts at: 3523 3523
Size: 6524
PmlI CAC'GTG
Cuts at: 1521 2367 1521
Size: 846 5678
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 5663
Size: 32 6492
PpuMI rG'GwC_Cy
Cuts at: 366 2244 2819 3123 366
Size: 1878 575 304 3767
Fragments arranged by size:
3767 1878 575 304
PshAI GACnn'nnGTC
Cuts at: 3325 3325
Size: 6524
PspOMI G'GGCC_C
Cuts at: 3208 3208
Size: 6524
PspXI vC'TCGA_Gb
Cuts at: 2937 2937
Size: 6524
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3142 3174 3142
Size: 32 6492
PstI C_TGCA'G
Cuts at: 413 2658 3017 3134 3292 3314 3571 4256 413
Size: 2245 359 117 158 22 257 685 2681
Fragments arranged by size:
2681 2245 685 359 257 158 117 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6524
PvuII CAG'CTG
Cuts at: 732 2617 2874 3949 4309 732
Size: 1885 257 1075 360 2947
Fragments arranged by size:
2947 1885 1075 360 257
RsrII CG'GwC_CG
Cuts at: 1158 4719 1158
Size: 3561 2963
SacI G_AGCT'C
Cuts at: 3538 3538
Size: 6524
SacII CC_GC'GG
Cuts at: 945 2663 945
Size: 1718 4806
SalI G'TCGA_C
Cuts at: 3561 3561
Size: 6524
SapI GCTCTTCn'nnn_
Cuts at: 4547 4757 4547
Size: 210 6314
SbfI CC_TGCA'GG
Cuts at: 3571 3571
Size: 6524
ScaI AGT'ACT
Cuts at: 6041 6041
Size: 6524
SexAI A'CCwGG_T
Cuts at: 3001 3001
Size: 6524
SfcI C'TryA_G
Cuts at: 33 409 2654 3013 3130 3288 3310 3567 4252
Size: 376 2245 359 117 158 22 257 685
Cuts at: 4252 5245 5436 33
Size: 993 191 1121
Fragments arranged by size:
2245 1121 993 685 376 359 257 191
158 117 22
SfoI GGC'GCC
Cuts at: 660 1326 1739 3441 3956 4204 660
Size: 666 413 1702 515 248 2980
Fragments arranged by size:
2980 1702 666 515 413 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
SgrAI Cr'CCGG_yG
Cuts at: 3380 3380
Size: 6524
SmaI CCC'GGG
Cuts at: 2068 2395 3546 2068
Size: 327 1151 5046
Fragments arranged by size:
5046 1151 327
SmlI C'TyrA_G
Cuts at: 2937 3679 4594 5086 5348 2937
Size: 742 915 492 262 4113
Fragments arranged by size:
4113 915 742 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6524
SphI G_CATG'C
Cuts at: 1448 3577 4077 1448
Size: 2129 500 3895
Fragments arranged by size:
3895 2129 500
SspI AAT'ATT
Cuts at: 850 850
Size: 6524
StuI AGG'CCT
Cuts at: 1576 2117 2521 1576
Size: 541 404 5579
Fragments arranged by size:
5579 541 404
StyI C'CwwG_G
Cuts at: 107 974 1535 1677 2312 2594 3658 4012 107
Size: 867 561 142 635 282 1064 354 2619
Fragments arranged by size:
2619 1064 867 635 561 354 282 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 600 1175 1507 1681 2150 4107 4240 4699 4908
Size: 575 332 174 469 1957 133 459 209
Cuts at: 4908 600
Size: 2216
Fragments arranged by size:
2216 1957 575 469 459 332 209 174
133
TatI w'GTAC_w
Cuts at: 419 746 1826 6039 419
Size: 327 1080 4213 904
Fragments arranged by size:
4213 1080 904 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2725 2757 6041 6073 2725
Size: 32 3284 32 3176
Fragments arranged by size:
3284 3176 32 32
Tth111I GACn'n_nGTC
Cuts at: 1111 4321 1111
Size: 3210 3314
XbaI T'CTAG_A
Cuts at: 3555 3555
Size: 6524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 839 893 3195 4019 324
Size: 515 54 2302 824 2829
Fragments arranged by size:
2829 2302 824 515 54
XhoI C'TCGA_G
Cuts at: 2937 2937
Size: 6524
XmaI C'CCGG_G
Cuts at: 2066 2393 3544 2066
Size: 327 1151 5046
Fragments arranged by size:
5046 1151 327
XmnI GAAnn'nnTTC
Cuts at: 1081 1264 1081
Size: 183 6341
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BssHII BstZ17I ClaI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PsiI SanDI SfiI SnaBI SrfI SwaI
ZraI