Restriction map for
pF1KE5338
(Circular) (Six-base) MAPSORT of: pF1KE5338.seq Check: 3532 from: 1 to: 3905
pF1KE5338 3905 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 551 551
Size: 3905
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 463 921 463
Size: 458 3447
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2162 2909 3177 1730
Size: 432 747 268 2458
Fragments arranged by size:
2458 747 432 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 637 637
Size: 3905
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 478 510 478
Size: 32 3873
AlwNI CAG_nnn'CTG
Cuts at: 427 1210 2777 427
Size: 783 1567 1555
Fragments arranged by size:
1567 1555 783
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 909 3258 3514 909
Size: 2349 256 1300
Fragments arranged by size:
2349 1300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 731 925 731
Size: 194 3711
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 118 463 600 921 1335 1583 1618 118
Size: 345 137 321 414 248 35 2405
Fragments arranged by size:
2405 414 345 321 248 137 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BciVI GTATCCnnnnn_n'
Cuts at: 729 1803 2564 3905 729
Size: 1074 761 1341 729
Fragments arranged by size:
1341 1074 761 729
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 121 272 794 1530 1623 2679 121
Size: 151 522 736 93 1056 1347
Fragments arranged by size:
1347 1056 736 522 151 93
BmgBI CAC'GTC
Cuts at: 483 483
Size: 3905
BmrI ACTGGGnnnn_n'
Cuts at: 117 579 1117 1198 1533 3677 117
Size: 462 538 81 335 2144 345
Fragments arranged by size:
2144 538 462 345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1960 2452 2750 1081
Size: 879 492 298 2236
Fragments arranged by size:
2236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 548 1336 1584 2286 3782 548
Size: 788 248 702 1496 671
Fragments arranged by size:
1496 788 702 671 248
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 111 141 478 508 111
Size: 30 337 30 3508
Fragments arranged by size:
3508 337 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 139
Size: 3905
BseYI C'CCAG_C
Cuts at: 271 316 595 608 870 1330 2088 2665 3161
Size: 45 279 13 262 460 758 577 496
Cuts at: 3161 271
Size: 1015
Fragments arranged by size:
1015 758 577 496 460 279 262 45
13
BsiEI CG_ry'CG
Cuts at: 104 810 1447 1493 2701 104
Size: 706 637 46 1208 1308
Fragments arranged by size:
1308 1208 706 637 46
BsiHKAI G_wGCw'C
Cuts at: 919 1697 1887 2679 919
Size: 778 190 792 2145
Fragments arranged by size:
2145 792 778 190
BsmI GAATG_Cn'
Cuts at: 294 294
Size: 3905
Bsp1286I G_dGCh'C
Cuts at: 121 272 794 919 1530 1623 1697 1887 1949
Size: 151 522 125 611 93 74 190 62
Cuts at: 1949 2679 121
Size: 730 1347
Fragments arranged by size:
1347 730 611 522 190 151 125 93
74 62
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 955 1169 1471 1852 826
Size: 129 214 302 381 2879
Fragments arranged by size:
2879 381 302 214 129
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 668 1817 668
Size: 1149 2756
BsrFI r'CCGG_y
Cuts at: 1903 2084 2293 1903
Size: 181 209 3515
Fragments arranged by size:
3515 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstXI CCAn_nnnn'nTGG
Cuts at: 778 778
Size: 3905
BstYI r'GATC_y
Cuts at: 1 312 424 930 1397 1420 1755 2001 3013
Size: 311 112 506 467 23 335 246 1012
Cuts at: 3013 3099 3111 3457 1
Size: 86 12 346 449
Fragments arranged by size:
1012 506 467 449 346 335 311 246
112 86 23 12
BtgI C'CryG_G
Cuts at: 777 777
Size: 3905
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 600 1855 2036 2199 600
Size: 1255 181 163 2306
Fragments arranged by size:
2306 1255 181 163
BtsI GCAGTG_nn'
Cuts at: 881 1148 881
Size: 267 3638
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DraIII CAC_nnn'GTG
Cuts at: 483 483
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 653 1490 1664 2055 2082 3427 653
Size: 837 174 391 27 1345 1131
Fragments arranged by size:
1345 1131 837 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2162 2909 3177 1730
Size: 432 747 268 2458
Fragments arranged by size:
2458 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1044 1044
Size: 3905
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 786 1339 1587 2609 3588 786
Size: 553 248 1022 979 1103
Fragments arranged by size:
1103 1022 979 553 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1747 1777 1779 1809 1747
Size: 30 2 30 3843
Fragments arranged by size:
3843 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 467 925 467
Size: 458 3447
MfeI C'AATT_G
Cuts at: 439 439
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2760 2576
Size: 184 3721
MscI TGG'CCA
Cuts at: 655 1666 655
Size: 1011 2894
MslI CAynn'nnrTG
Cuts at: 776 1894 2021 776
Size: 1118 127 2660
Fragments arranged by size:
2660 1118 127
MspA1I CmG'CkG
Cuts at: 714 1016 1330 1690 2703 2948 714
Size: 302 314 360 1013 245 1671
Fragments arranged by size:
1671 1013 360 314 302 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 777 777
Size: 3905
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PflMI CCAn_nnn'nTGG
Cuts at: 483 483
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 478 510 3044 3076 478
Size: 32 2534 32 1307
Fragments arranged by size:
2534 1307 32 32
PsiI TTA'TAA
Cuts at: 377 377
Size: 3905
PstI C_TGCA'G
Cuts at: 344 837 952 1637 344
Size: 493 115 685 2612
Fragments arranged by size:
2612 685 493 115
PvuI CG_AT'CG
Cuts at: 104 810 104
Size: 706 3199
PvuII CAG'CTG
Cuts at: 714 1330 1690 714
Size: 616 360 2929
Fragments arranged by size:
2929 616 360
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 340 833 948 1633 2626 2817 33
Size: 307 493 115 685 993 191 1121
Fragments arranged by size:
1121 993 685 493 307 191 115
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 733 927 733
Size: 194 3711
SmlI C'TyrA_G
Cuts at: 1060 1975 2467 2729 1060
Size: 915 492 262 2236
Fragments arranged by size:
2236 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
SspI AAT'ATT
Cuts at: 408 408
Size: 3905
StyI C'CwwG_G
Cuts at: 674 777 1039 1393 674
Size: 103 262 354 3186
Fragments arranged by size:
3186 354 262 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 136 790 1488 1621 2080 2289 136
Size: 654 698 133 459 209 1752
Fragments arranged by size:
1752 698 654 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 111 143 416 448 3422 3454 111
Size: 32 273 32 2974 32 562
Fragments arranged by size:
2974 562 273 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 592 1400 592
Size: 808 3097
XmaI C'CCGG_G
Cuts at: 731 925 731
Size: 194 3711
ZraI GAC'GTC
Cuts at: 549 549
Size: 3905
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI