Restriction map for
pF1KE5408
(Circular) (Six-base) MAPSORT of: pF1KE5408.seq Check: 7477 from: 1 to: 4121
pF1KE5408 4121 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 627 627
Size: 4121
AccI GT'mk_AC
Cuts at: 1159 1159
Size: 4121
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1946 2378 3125 3393 304
Size: 1642 432 747 268 1032
Fragments arranged by size:
1642 1032 747 432 268
AflIII A'CryG_T
Cuts at: 856 1473 2577 3554 856
Size: 617 1104 977 1423
Fragments arranged by size:
1423 1104 977 617
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AhdI GACnn_n'nnGTC
Cuts at: 1002 1002
Size: 4121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 120 273 330 342 1426 2993 120
Size: 153 57 12 1084 1567 1248
Fragments arranged by size:
1567 1248 1084 153 57 12
ApaLI G'TGCA_C
Cuts at: 580 979 2891 580
Size: 399 1912 1810
Fragments arranged by size:
1912 1810 399
ApoI r'AATT_y
Cuts at: 826 931 1125 3474 3730 826
Size: 105 194 2349 256 1217
Fragments arranged by size:
2349 1217 256 194 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 396 1141 396
Size: 745 3376
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 155 1137 1551 1799 1834 155
Size: 982 414 248 35 2442
Fragments arranged by size:
2442 982 414 248 35
BanII G_rGCy'C
Cuts at: 839 1135 2165 839
Size: 296 1030 2795
Fragments arranged by size:
2795 1030 296
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BbsI GAAGACnn'nnnn_
Cuts at: 204 219 705 204
Size: 15 486 3620
Fragments arranged by size:
3620 486 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4087
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 1641 1641
Size: 4121
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 385 1233 385
Size: 848 3273
Bme1580I G_kGCm'C
Cuts at: 482 584 983 1746 1839 2895 482
Size: 102 399 763 93 1056 1708
Fragments arranged by size:
1708 1056 763 399 102 93
BmgBI CAC'GTC
Cuts at: 585 585
Size: 4121
BmrI ACTGGGnnnn_n'
Cuts at: 420 1074 1333 1414 1749 3893 420
Size: 654 259 81 335 2144 648
Fragments arranged by size:
2144 654 648 335 259 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 376 745 352
Size: 24 369 3728
Fragments arranged by size:
3728 369 24
Bpu10I CC'TnA_GC
Cuts at: 775 775
Size: 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 857 1474 857
Size: 617 3504
BsaBI GATnn'nnATC
Cuts at: 6 850 1635 3672 6
Size: 844 785 2037 455
Fragments arranged by size:
2037 844 785 455
BsaHI Gr'CG_yC
Cuts at: 1552 1800 2502 3998 1552
Size: 248 702 1496 1675
Fragments arranged by size:
1675 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1310 1831 2509 2783 2930 3341 1310
Size: 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 406 436 295
Size: 30 81 30 3980
Fragments arranged by size:
3980 81 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 222 316 1002 222
Size: 94 686 3341
Fragments arranged by size:
3341 686 94
BseYI C'CCAG_C
Cuts at: 189 620 1546 2304 2881 3377 189
Size: 431 926 758 577 496 933
Fragments arranged by size:
933 926 758 577 496 431
BsiEI CG_ry'CG
Cuts at: 104 707 1663 1709 2917 104
Size: 603 956 46 1208 1308
Fragments arranged by size:
1308 1208 956 603 46
BsiHKAI G_wGCw'C
Cuts at: 308 386 452 584 665 781 839 983 1135
Size: 78 66 132 81 116 58 144 152
Cuts at: 1135 1913 2103 2895 308
Size: 778 190 792 1534
Fragments arranged by size:
1534 792 778 190 152 144 132 116
81 78 66 58
BsmI GAATG_Cn'
Cuts at: 530 530
Size: 4121
Bsp1286I G_dGCh'C
Cuts at: 308 386 452 482 584 665 781 839 983
Size: 78 66 30 102 81 116 58 144
Cuts at: 983 1135 1746 1839 1913 2103 2165 2895 308
Size: 152 611 93 74 190 62 730 1534
Fragments arranged by size:
1534 730 611 190 152 144 116 102
93 81 78 74 66 62 58 30
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 3297 3297
Size: 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 627 872 1171 1385 1687 2068 266
Size: 361 245 299 214 302 381 2319
Fragments arranged by size:
2319 381 361 302 299 245 214
BsrBI CCG'CTC
Cuts at: 50 153 161 2413 2467 3951 4116 50
Size: 103 8 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 103 55 54 8
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 124 597 2119 2300 2509 124
Size: 473 1522 181 209 1736
Fragments arranged by size:
1736 1522 473 209 181
BssSI C'ACGA_G
Cuts at: 132 307 2392 2750 132
Size: 175 2085 358 1503
Fragments arranged by size:
2085 1503 358 175
BstAPI GCAn_nnn'nTGC
Cuts at: 134 1498 134
Size: 1364 2757
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstXI CCAn_nnnn'nTGG
Cuts at: 639 789 1033 639
Size: 150 244 3727
Fragments arranged by size:
3727 244 150
BstYI r'GATC_y
Cuts at: 1 1146 1613 1636 1971 2217 3229 3315 3327
Size: 1145 467 23 335 246 1012 86 12
Cuts at: 3327 3673 1
Size: 346 449
Fragments arranged by size:
1145 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 227 1364 227
Size: 1137 2984
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DraIII CAC_nnn'GTG
Cuts at: 477 477
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 122 468 644 704 1073 1706 1880 2271 2298
Size: 346 176 60 369 633 174 391 27
Cuts at: 2298 3643 122
Size: 1345 600
Fragments arranged by size:
1345 633 600 391 369 346 176 174
60 27
EagI C'GGCC_G
Cuts at: 704 1706 704
Size: 1002 3119
EarI CTCTTCn'nnn_
Cuts at: 436 2144 2354 436
Size: 1708 210 2203
Fragments arranged by size:
2203 1708 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2329 2639 2785 3931 2329
Size: 310 146 1146 2519
Fragments arranged by size:
2519 1146 310 146
EcoICRI GAG'CTC
Cuts at: 837 1133 837
Size: 296 3825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 352 376 745 1946 2378 3125 3393 304
Size: 48 24 369 1201 432 747 268 1032
Fragments arranged by size:
1201 1032 747 432 369 268 48 24
EcoNI CCTnn'n_nnAGG
Cuts at: 809 809
Size: 4121
EcoO109I rG'GnC_Cy
Cuts at: 255 1260 255
Size: 1005 3116
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 309 2140 309
Size: 1831 2290
FspI TGC'GCA
Cuts at: 277 1902 277
Size: 1625 2496
HaeII r_GCGC'y
Cuts at: 1555 1803 2825 3804 1555
Size: 248 1022 979 1872
Fragments arranged by size:
1872 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 406 438 988 1020 1963 1993 1995
Size: 32 73 32 550 32 943 30 2
Cuts at: 1995 2025 301
Size: 30 2397
Fragments arranged by size:
2397 943 550 73 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2792 2976 2792
Size: 184 3937
MscI TGG'CCA
Cuts at: 470 646 1075 1882 470
Size: 176 429 807 2709
Fragments arranged by size:
2709 807 429 176
MslI CAynn'nnrTG
Cuts at: 577 2110 2237 577
Size: 1533 127 2461
Fragments arranged by size:
2461 1533 127
MspA1I CmG'CkG
Cuts at: 330 620 709 1033 1232 1546 1906 2919 3164
Size: 290 89 324 199 314 360 1013 245
Cuts at: 3164 330
Size: 1287
Fragments arranged by size:
1287 1013 360 324 314 290 245 199
89
NaeI GCC'GGC
Cuts at: 126 2302 126
Size: 2176 1945
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NgoMIV G'CCGG_C
Cuts at: 124 2300 124
Size: 2176 1945
NspI r_CATG'y
Cuts at: 1174 1674 2581 1174
Size: 500 907 2714
Fragments arranged by size:
2714 907 500
PciI A'CATG_T
Cuts at: 2577 2577
Size: 4121
PfoI T'CCnGG_A
Cuts at: 722 743 722
Size: 21 4100
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PmlI CAC'GTG
Cuts at: 857 857
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PpuMI rG'GwC_Cy
Cuts at: 255 255
Size: 4121
PstI C_TGCA'G
Cuts at: 116 1168 1853 116
Size: 1052 685 2384
Fragments arranged by size:
2384 1052 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 330 1033 1546 1906 330
Size: 703 513 360 2545
Fragments arranged by size:
2545 703 513 360
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 839 1135 839
Size: 296 3825
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 436 2144 2354 436
Size: 1708 210 2203
Fragments arranged by size:
2203 1708 210
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SexAI A'CCwGG_T
Cuts at: 206 206
Size: 4121
SfcI C'TryA_G
Cuts at: 33 112 1164 1849 2842 3033 33
Size: 79 1052 685 993 191 1121
Fragments arranged by size:
1121 1052 993 685 191 79
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
StuI AGG'CCT
Cuts at: 502 502
Size: 4121
StyI C'CwwG_G
Cuts at: 666 1255 1609 666
Size: 589 354 3178
Fragments arranged by size:
3178 589 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1093 1704 1837 2296 2505 1093
Size: 611 133 459 209 2709
Fragments arranged by size:
2709 611 459 209 133
TatI w'GTAC_w
Cuts at: 3636 3636
Size: 4121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 3638 3670 295
Size: 32 3311 32 746
Fragments arranged by size:
3311 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 639 1616 639
Size: 977 3144
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
XmnI GAAnn'nnTTC
Cuts at: 931 931
Size: 4121
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI