Restriction map for
pF1KE5416
(Circular) (Six-base) MAPSORT of: pF1KE5416.seq Check: 3781 from: 1 to: 4151
pF1KE5416 4151 bp
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 118 118
Size: 4151
AatII G_ACGT'C
Cuts at: 287 287
Size: 4151
AccI GT'mk_AC
Cuts at: 611 1189 611
Size: 578 3573
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1976 2408 3155 3423 1976
Size: 432 747 268 2704
Fragments arranged by size:
2704 747 432 268
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AleI CACnn'nnGTG
Cuts at: 850 850
Size: 4151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1061 1061
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 198 1456 3023 198
Size: 1258 1567 1326
Fragments arranged by size:
1567 1326 1258
ApaI G_GGCC'C
Cuts at: 1061 1061
Size: 4151
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 697 1155 3504 3760 697
Size: 458 2349 256 1088
Fragments arranged by size:
2349 1088 458 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 707 974 1171 707
Size: 267 197 3687
Fragments arranged by size:
3687 267 197
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 130 1003 1167 1581 1829 1864 130
Size: 873 164 414 248 35 2417
Fragments arranged by size:
2417 873 414 248 164 35
BanII G_rGCy'C
Cuts at: 845 989 1061 1165 2195 845
Size: 144 72 104 1030 2801
Fragments arranged by size:
2801 1030 144 104 72
BbeI G_GCGC'C
Cuts at: 1007 1585 1833 1007
Size: 578 248 3325
Fragments arranged by size:
3325 578 248
BbsI GAAGACnn'nnnn_
Cuts at: 262 262
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 1052 2049 2810 4151 1052
Size: 997 761 1341 1052
Fragments arranged by size:
1341 1052 997 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglI GCCn_nnn'nGGC
Cuts at: 304 304
Size: 4151
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 135 1061 1776 1869 2925 135
Size: 926 715 93 1056 1361
Fragments arranged by size:
1361 1056 926 715 93
BmrI ACTGGGnnnn_n'
Cuts at: 886 1363 1444 1779 3923 886
Size: 477 81 335 2144 1114
Fragments arranged by size:
2144 1114 477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 821 1019 220
Size: 601 198 3352
Fragments arranged by size:
3352 601 198
Bpu10I CC'TnA_GC
Cuts at: 434 544 434
Size: 110 4041
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2698 2996 1327
Size: 879 492 298 2482
Fragments arranged by size:
2482 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 307 891 3855 307
Size: 584 2964 603
Fragments arranged by size:
2964 603 584
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 284 1004 1582 1830 2532 4028 284
Size: 720 578 248 702 1496 407
Fragments arranged by size:
1496 720 702 578 407 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 955 976 985 1006 223
Size: 30 702 21 9 21 3368
Fragments arranged by size:
3368 702 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 997 200
Size: 797 3354
BseYI C'CCAG_C
Cuts at: 1061 1576 2334 2911 3407 1061
Size: 515 758 577 496 1805
Fragments arranged by size:
1805 758 577 515 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 273 687 238
Size: 35 414 3702
Fragments arranged by size:
3702 414 35
BsiEI CG_ry'CG
Cuts at: 104 581 1693 1739 2947 104
Size: 477 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 477 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1943 2133 2925 1165
Size: 778 190 792 2391
Fragments arranged by size:
2391 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 135 845 989 1061 1165 1776 1869 1943 2133
Size: 710 144 72 104 611 93 74 190
Cuts at: 2133 2195 2925 135
Size: 62 730 1361
Fragments arranged by size:
1361 730 710 611 190 144 104 93
74 72 62
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 118 478 1201 1415 1717 2098 118
Size: 360 723 214 302 381 2171
Fragments arranged by size:
2171 723 381 360 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 433 2063 433
Size: 1630 2521
BsrFI r'CCGG_y
Cuts at: 576 2149 2330 2539 576
Size: 1573 181 209 2188
Fragments arranged by size:
2188 1573 209 181
BssHII G'CGCG_C
Cuts at: 269 269
Size: 4151
BssSI C'ACGA_G
Cuts at: 964 2422 2780 964
Size: 1458 358 2335
Fragments arranged by size:
2335 1458 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 519 852 519
Size: 333 3818
BstYI r'GATC_y
Cuts at: 1 390 1176 1643 1666 2001 2247 3259 3345
Size: 389 786 467 23 335 246 1012 86
Cuts at: 3345 3357 3703 1
Size: 12 346 449
Fragments arranged by size:
1012 786 467 449 389 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 299 321 381 1124 107
Size: 192 22 60 743 3134
Fragments arranged by size:
3134 743 192 60 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 554 2101 2282 2445 554
Size: 1547 181 163 2260
Fragments arranged by size:
2260 1547 181 163
BtsI GCAGTG_nn'
Cuts at: 347 1394 347
Size: 1047 3104
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 634 1857 2715 634
Size: 1223 858 2070
Fragments arranged by size:
2070 1223 858
EaeI y'GGCC_r
Cuts at: 1011 1121 1736 1910 2301 2328 3673 1011
Size: 110 615 174 391 27 1345 1489
Fragments arranged by size:
1489 1345 615 391 174 110 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 821 1019 1976 2408 3155 3423 220
Size: 601 198 957 432 747 268 948
Fragments arranged by size:
957 948 747 601 432 268 198
EcoNI CCTnn'n_nnAGG
Cuts at: 1049 1049
Size: 4151
EcoO109I rG'GnC_Cy
Cuts at: 195 761 1057 1058 1290 195
Size: 566 296 1 232 3056
Fragments arranged by size:
3056 566 296 232 1
EcoRI G'AATT_C
Cuts at: 697 1155 697
Size: 458 3693
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 639 813 2170 639
Size: 174 1357 2620
Fragments arranged by size:
2620 1357 174
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 478 931 1007 1585 1833 2855 3834 478
Size: 453 76 578 248 1022 979 795
Fragments arranged by size:
1022 979 795 578 453 248 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 1993 2023 2025 2055 223
Size: 32 1738 30 2 30 2319
Fragments arranged by size:
2319 1738 32 30 30 2
HincII GTy'rAC
Cuts at: 995 1190 995
Size: 195 3956
KasI G'GCGC_C
Cuts at: 1003 1581 1829 1003
Size: 578 248 3325
Fragments arranged by size:
3325 578 248
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 1013 1912 1013
Size: 899 3252
MslI CAynn'nnrTG
Cuts at: 850 2140 2267 850
Size: 1290 127 2734
Fragments arranged by size:
2734 1290 127
MspA1I CmG'CkG
Cuts at: 212 383 420 533 767 1009 1099 1262 1576
Size: 171 37 113 234 242 90 163 314
Cuts at: 1576 1936 2949 3194 212
Size: 360 1013 245 1169
Fragments arranged by size:
1169 1013 360 314 245 242 234 171
163 113 90 37
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1004 1582 1830 1004
Size: 578 248 3325
Fragments arranged by size:
3325 578 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PasI CC'CwG_GG
Cuts at: 727 727
Size: 4151
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 146 146
Size: 4151
PfoI T'CCnGG_A
Cuts at: 689 689
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 761 761
Size: 4151
PspOMI G'GGCC_C
Cuts at: 1057 1057
Size: 4151
PstI C_TGCA'G
Cuts at: 156 425 1198 1883 156
Size: 269 773 685 2424
Fragments arranged by size:
2424 773 685 269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 212 420 533 1099 1576 1936 212
Size: 208 113 566 477 360 2427
Fragments arranged by size:
2427 566 477 360 208 113
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SacII CC_GC'GG
Cuts at: 384 384
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SanDI GG'GwC_CC
Cuts at: 761 761
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 152 207 421 528 1194 1879 2872 3063
Size: 119 55 214 107 666 685 993 191
Cuts at: 3063 33
Size: 1121
Fragments arranged by size:
1121 993 685 666 214 191 119 107
55
SfoI GGC'GCC
Cuts at: 1005 1583 1831 1005
Size: 578 248 3325
Fragments arranged by size:
3325 578 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 976 1173 976
Size: 197 3954
SmlI C'TyrA_G
Cuts at: 1306 2221 2713 2975 1306
Size: 915 492 262 2482
Fragments arranged by size:
2482 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StuI AGG'CCT
Cuts at: 970 970
Size: 4151
StyI C'CwwG_G
Cuts at: 107 1285 1639 107
Size: 1178 354 2619
Fragments arranged by size:
2619 1178 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 806 838 955 987 3668 3700 114
Size: 32 660 32 117 32 2681 32 565
Fragments arranged by size:
2681 660 565 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 663 1948 663
Size: 1285 2866
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1101 1646 1101
Size: 545 3606
XmaI C'CCGG_G
Cuts at: 974 1171 974
Size: 197 3954
ZraI GAC'GTC
Cuts at: 285 285
Size: 4151
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI