Restriction map for
pF1KE5454
(Circular) (Six-base) MAPSORT of: pF1KE5454.seq Check: 2078 from: 1 to: 4172
pF1KE5454 4172 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1210 1210
Size: 4172
Acc65I G'GTAC_C
Cuts at: 1188 1188
Size: 4172
AclI AA'CG_TT
Cuts at: 4012 4012
Size: 4172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 1997 2429 3176 3444 129
Size: 1868 432 747 268 857
Fragments arranged by size:
1868 857 747 432 268
AflIII A'CryG_T
Cuts at: 207 1524 2628 3605 207
Size: 1317 1104 977 774
Fragments arranged by size:
1317 1104 977 774
AgeI A'CCGG_T
Cuts at: 2560 2560
Size: 4172
AleI CACnn'nnGTG
Cuts at: 628 628
Size: 4172
AlwNI CAG_nnn'CTG
Cuts at: 1477 3044 1477
Size: 1567 2605
ApaLI G'TGCA_C
Cuts at: 2942 2942
Size: 4172
ApoI r'AATT_y
Cuts at: 707 1176 3525 3781 707
Size: 469 2349 256 1098
Fragments arranged by size:
2349 1098 469 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
AvaI C'yCGr_G
Cuts at: 1162 1192 1162
Size: 30 4142
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 909 942 909
Size: 33 4139
BamHI G'GATC_C
Cuts at: 1197 1197
Size: 4172
BanI G'GyrC_C
Cuts at: 1014 1188 1602 1850 1885 1014
Size: 174 414 248 35 3301
Fragments arranged by size:
3301 414 248 174 35
BanII G_rGCy'C
Cuts at: 671 911 1186 2216 671
Size: 240 275 1030 2627
Fragments arranged by size:
2627 1030 275 240
BbeI G_GCGC'C
Cuts at: 1606 1854 1606
Size: 248 3924
BbsI GAAGACnn'nnnn_
Cuts at: 135 135
Size: 4172
BbvCI CC'TCA_GC
Cuts at: 263 1154 263
Size: 891 3281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1131 1165 1131
Size: 34 4138
BciVI GTATCCnnnnn_n'
Cuts at: 2070 2831 4172 2070
Size: 761 1341 2070
Fragments arranged by size:
2070 1341 761
BclI T'GATC_A
Cuts at: 396 1692 396
Size: 1296 2876
BglII A'GATC_T
Cuts at: 1687 3724 1687
Size: 2037 2135
BlpI GC'TnA_GC
Cuts at: 1284 1284
Size: 4172
Bme1580I G_kGCm'C
Cuts at: 1797 1890 2946 1797
Size: 93 1056 3023
Fragments arranged by size:
3023 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1384 1465 1800 3944 1384
Size: 81 335 2144 1612
Fragments arranged by size:
2144 1612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1086 2539 1086
Size: 1453 2719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1107 1107
Size: 4172
Bpu10I CC'TnA_GC
Cuts at: 263 1154 263
Size: 891 3281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1348 2227 2719 3017 179
Size: 1169 879 492 298 1334
Fragments arranged by size:
1334 1169 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 655 3876 655
Size: 3221 951
BsaAI yAC'GTr
Cuts at: 1525 1525
Size: 4172
BsaBI GATnn'nnATC
Cuts at: 6 1686 3723 6
Size: 1680 2037 455
Fragments arranged by size:
2037 1680 455
BsaHI Gr'CG_yC
Cuts at: 1603 1851 2553 4049 1603
Size: 248 702 1496 1726
Fragments arranged by size:
1726 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1361 1882 2560 2834 2981 3392 1361
Size: 521 678 274 147 411 2141
Fragments arranged by size:
2141 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 966 966
Size: 4172
BseYI C'CCAG_C
Cuts at: 1597 2355 2932 3428 1597
Size: 758 577 496 2341
Fragments arranged by size:
2341 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 619 481
Size: 138 4034
BsiEI CG_ry'CG
Cuts at: 104 1714 1760 2968 104
Size: 1610 46 1208 1308
Fragments arranged by size:
1610 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 671 1186 1964 2154 2946 671
Size: 515 778 190 792 1897
Fragments arranged by size:
1897 792 778 515 190
BsmI GAATG_Cn'
Cuts at: 274 274
Size: 4172
Bsp1286I G_dGCh'C
Cuts at: 671 911 1186 1797 1890 1964 2154 2216 2946
Size: 240 275 611 93 74 190 62 730
Cuts at: 2946 671
Size: 1897
Fragments arranged by size:
1897 730 611 275 240 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3392 3392
Size: 4172
BspHI T'CATG_A
Cuts at: 3348 3348
Size: 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 729 1222 1436 1738 2119 729
Size: 493 214 302 381 2782
Fragments arranged by size:
2782 493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2464 2518 4002 4167 50
Size: 2414 54 1484 165 55
Fragments arranged by size:
2414 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 313 480 1100 2084 313
Size: 167 620 984 2401
Fragments arranged by size:
2401 984 620 167
BsrFI r'CCGG_y
Cuts at: 2170 2351 2560 2170
Size: 181 209 3782
Fragments arranged by size:
3782 209 181
BssSI C'ACGA_G
Cuts at: 2443 2801 2443
Size: 358 3814
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1549 1123
Size: 426 3746
BstBI TT'CG_AA
Cuts at: 2533 2533
Size: 4172
BstYI r'GATC_y
Cuts at: 1 1197 1664 1687 2022 2268 3280 3366 3378
Size: 1196 467 23 335 246 1012 86 12
Cuts at: 3378 3724 1
Size: 346 449
Fragments arranged by size:
1196 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2122 2303 2466 2122
Size: 181 163 3828
Fragments arranged by size:
3828 181 163
BtsI GCAGTG_nn'
Cuts at: 464 1112 1415 464
Size: 648 303 3221
Fragments arranged by size:
3221 648 303
DraI TTT'AAA
Cuts at: 824 1171 824
Size: 347 3825
DraIII CAC_nnn'GTG
Cuts at: 519 519
Size: 4172
DrdI GACnn_nn'nnGTC
Cuts at: 1878 2736 1878
Size: 858 3314
EaeI y'GGCC_r
Cuts at: 1757 1931 2322 2349 3694 1757
Size: 174 391 27 1345 2235
Fragments arranged by size:
2235 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1757 1757
Size: 4172
EarI CTCTTCn'nnn_
Cuts at: 573 2195 2405 573
Size: 1622 210 2340
Fragments arranged by size:
2340 1622 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2380 2690 2836 3982 2380
Size: 310 146 1146 2570
Fragments arranged by size:
2570 1146 310 146
EcoICRI GAG'CTC
Cuts at: 669 1184 669
Size: 515 3657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 1107 1997 2429 3176 3444 129
Size: 978 890 432 747 268 857
Fragments arranged by size:
978 890 857 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1311 1311
Size: 4172
EcoRI G'AATT_C
Cuts at: 1176 1176
Size: 4172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1162 2191 1162
Size: 1029 3143
FspI TGC'GCA
Cuts at: 1953 1953
Size: 4172
HaeII r_GCGC'y
Cuts at: 1606 1854 2876 3855 1606
Size: 248 1022 979 1923
Fragments arranged by size:
1923 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 1094 1126 2014 2044 2046 2076 388
Size: 32 674 32 888 30 2 30 2484
Fragments arranged by size:
2484 888 674 32 32 30 30 2
HincII GTy'rAC
Cuts at: 930 1211 930
Size: 281 3891
HpaI GTT'AAC
Cuts at: 930 930
Size: 4172
KasI G'GCGC_C
Cuts at: 1602 1850 1602
Size: 248 3924
KpnI G_GTAC'C
Cuts at: 1192 1192
Size: 4172
MluI A'CGCG_T
Cuts at: 3605 3605
Size: 4172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3027 2843
Size: 184 3988
MscI TGG'CCA
Cuts at: 1933 1933
Size: 4172
MslI CAynn'nnrTG
Cuts at: 628 2161 2288 628
Size: 1533 127 2512
Fragments arranged by size:
2512 1533 127
MspA1I CmG'CkG
Cuts at: 402 506 1283 1597 1957 2970 3215 402
Size: 104 777 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 777 360 314 245 104
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 4172
NarI GG'CG_CC
Cuts at: 1603 1851 1603
Size: 248 3924
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 4172
NspI r_CATG'y
Cuts at: 211 1225 1725 2632 211
Size: 1014 500 907 1751
Fragments arranged by size:
1751 1014 907 500
PciI A'CATG_T
Cuts at: 207 2628 207
Size: 2421 1751
PflMI CCAn_nnn'nTGG
Cuts at: 807 807
Size: 4172
PmeI GTTT'AAAC
Cuts at: 1171 1171
Size: 4172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 4140
PstI C_TGCA'G
Cuts at: 1219 1904 1219
Size: 685 3487
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4172
PvuII CAG'CTG
Cuts at: 402 1597 1957 402
Size: 1195 360 2617
Fragments arranged by size:
2617 1195 360
RsrII CG'GwC_CG
Cuts at: 2367 2367
Size: 4172
SacI G_AGCT'C
Cuts at: 671 1186 671
Size: 515 3657
SalI G'TCGA_C
Cuts at: 1209 1209
Size: 4172
SapI GCTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
SbfI CC_TGCA'GG
Cuts at: 1219 1219
Size: 4172
ScaI AGT'ACT
Cuts at: 3689 3689
Size: 4172
SexAI A'CCwGG_T
Cuts at: 515 593 515
Size: 78 4094
SfcI C'TryA_G
Cuts at: 33 1215 1900 2893 3084 33
Size: 1182 685 993 191 1121
Fragments arranged by size:
1182 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1604 1852 1604
Size: 248 3924
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
SmaI CCC'GGG
Cuts at: 1194 1194
Size: 4172
SmlI C'TyrA_G
Cuts at: 194 1327 2242 2734 2996 194
Size: 1133 915 492 262 1370
Fragments arranged by size:
1370 1133 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4172
SphI G_CATG'C
Cuts at: 1225 1725 1225
Size: 500 3672
SspI AAT'ATT
Cuts at: 950 950
Size: 4172
StyI C'CwwG_G
Cuts at: 1306 1660 1306
Size: 354 3818
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1755 1888 2347 2556 1755
Size: 133 459 209 3371
Fragments arranged by size:
3371 459 209 133
TatI w'GTAC_w
Cuts at: 3687 3687
Size: 4172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4140
Tth111I GACn'n_nGTC
Cuts at: 1969 1969
Size: 4172
XbaI T'CTAG_A
Cuts at: 1203 1203
Size: 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 591 1667 591
Size: 1076 3096
XmaI C'CCGG_G
Cuts at: 1192 1192
Size: 4172
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI