Restriction map for
pF1KE5553
(Circular) (Six-base) MAPSORT of: pF1KE5553.seq Check: 8196 from: 1 to: 4391
pF1KE5553 4391 bp
With 182 enzymes: SgfI *
September 30, 2015 09:49 ..
AccI GT'mk_AC
Cuts at: 983 1429 983
Size: 446 3945
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 795 1094 2216 2648 3395 3663 178
Size: 617 299 1122 432 747 268 906
Fragments arranged by size:
1122 906 747 617 432 299 268
AflII C'TTAA_G
Cuts at: 1150 1150
Size: 4391
AflIII A'CryG_T
Cuts at: 137 1743 2847 3824 137
Size: 1606 1104 977 704
Fragments arranged by size:
1606 1104 977 704
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 124 156 163 195 124
Size: 32 7 32 4320
Fragments arranged by size:
4320 32 32 7
AlwNI CAG_nnn'CTG
Cuts at: 1696 3263 1696
Size: 1567 2824
ApaLI G'TGCA_C
Cuts at: 3161 3161
Size: 4391
ApoI r'AATT_y
Cuts at: 720 1395 3744 4000 720
Size: 675 2349 256 1111
Fragments arranged by size:
2349 1111 675 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 1411 1411
Size: 4391
BamHI G'GATC_C
Cuts at: 860 1416 860
Size: 556 3835
BanI G'GyrC_C
Cuts at: 1407 1821 2069 2104 1407
Size: 414 248 35 3694
Fragments arranged by size:
3694 414 248 35
BanII G_rGCy'C
Cuts at: 167 176 1304 1405 2435 167
Size: 9 1128 101 1030 2123
Fragments arranged by size:
2123 1128 1030 101 9
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbsI GAAGACnn'nnnn_
Cuts at: 715 1018 715
Size: 303 4088
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1223 1911 1223
Size: 688 3703
BglII A'GATC_T
Cuts at: 278 1906 3943 278
Size: 1628 2037 726
Fragments arranged by size:
2037 1628 726
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 887 2016 2109 3165 887
Size: 1129 93 1056 2113
Fragments arranged by size:
2113 1129 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1684 2019 4163 1603
Size: 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1180 217
Size: 963 3428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 2446 2938 3236 1567
Size: 879 492 298 2722
Fragments arranged by size:
2722 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 138 224 1744 138
Size: 86 1520 2785
Fragments arranged by size:
2785 1520 86
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 330 1822 2070 2772 4268 330
Size: 1492 248 702 1496 453
Fragments arranged by size:
1496 1492 702 453 248
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3053 3200 3611 1580
Size: 521 678 274 147 411 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 124 154 163 193 124
Size: 30 9 30 4322
Fragments arranged by size:
4322 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 494 810 1291 494
Size: 316 481 3594
Fragments arranged by size:
3594 481 316
BseYI C'CCAG_C
Cuts at: 359 1816 2574 3151 3647 359
Size: 1457 758 577 496 1103
Fragments arranged by size:
1457 1103 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 864 864
Size: 4391
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3187 104
Size: 1829 46 1208 1308
Fragments arranged by size:
1829 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1304 1405 2183 2373 3165 1304
Size: 101 778 190 792 2530
Fragments arranged by size:
2530 792 778 190 101
BsmI GAATG_Cn'
Cuts at: 1359 1359
Size: 4391
Bsp1286I G_dGCh'C
Cuts at: 167 176 887 1304 1405 2016 2109 2183 2373
Size: 9 711 417 101 611 93 74 190
Cuts at: 2373 2435 3165 167
Size: 62 730 1393
Fragments arranged by size:
1393 730 711 611 417 190 101 93
74 62 9
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 479 1441 1655 1957 2338 479
Size: 962 214 302 381 2532
Fragments arranged by size:
2532 962 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 227 908 2303 227
Size: 681 1395 2315
Fragments arranged by size:
2315 1395 681
BsrFI r'CCGG_y
Cuts at: 2389 2570 2779 2389
Size: 181 209 4001
Fragments arranged by size:
4001 209 181
BssSI C'ACGA_G
Cuts at: 2662 3020 2662
Size: 358 4033
BstAPI GCAn_nnn'nTGC
Cuts at: 583 1768 583
Size: 1185 3206
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstYI r'GATC_y
Cuts at: 1 278 860 1416 1883 1906 2241 2487 3499
Size: 277 582 556 467 23 335 246 1012
Cuts at: 3499 3585 3597 3943 1
Size: 86 12 346 449
Fragments arranged by size:
1012 582 556 467 449 346 335 277
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 128 167 914 974 107
Size: 21 39 747 60 3524
Fragments arranged by size:
3524 747 60 39 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 99 2341 2522 2685 99
Size: 2242 181 163 1805
Fragments arranged by size:
2242 1805 181 163
BtsI GCAGTG_nn'
Cuts at: 385 1182 1634 385
Size: 797 452 3142
Fragments arranged by size:
3142 797 452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 637 672 637
Size: 35 4356
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DrdI GACnn_nn'nnGTC
Cuts at: 217 2097 2955 217
Size: 1880 858 1653
Fragments arranged by size:
1880 1653 858
EaeI y'GGCC_r
Cuts at: 110 125 1976 2150 2541 2568 3913 110
Size: 15 1851 174 391 27 1345 588
Fragments arranged by size:
1851 1345 588 391 174 27 15
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 1111 2414 2624 1111
Size: 1303 210 2878
Fragments arranged by size:
2878 1303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2599 2909 3055 4201 2599
Size: 310 146 1146 2789
Fragments arranged by size:
2789 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1302 1403 1302
Size: 101 4290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 217 795 1094 1180 2216 2648 3395 3663
Size: 39 578 299 86 1036 432 747 268
Cuts at: 3663 178
Size: 906
Fragments arranged by size:
1036 906 747 578 432 299 268 86
39
EcoO109I rG'GnC_Cy
Cuts at: 1530 1530
Size: 4391
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 1825 2073 3095 4074 1825
Size: 248 1022 979 2142
Fragments arranged by size:
2142 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1120 1152 1164 1196 2233 2263 2265 2295 1120
Size: 32 12 32 1037 30 2 30 3216
Fragments arranged by size:
3216 1037 32 32 30 30 12 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MfeI C'AATT_G
Cuts at: 689 689
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1029 3062 3246 1029
Size: 2033 184 2174
Fragments arranged by size:
2174 2033 184
MscI TGG'CCA
Cuts at: 112 127 2152 112
Size: 15 2025 2351
Fragments arranged by size:
2351 2025 15
MslI CAynn'nnrTG
Cuts at: 232 913 1143 2380 2507 232
Size: 681 230 1237 127 2116
Fragments arranged by size:
2116 1237 681 230 127
MspA1I CmG'CkG
Cuts at: 480 499 1502 1816 2176 3189 3434 480
Size: 19 1003 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 1003 360 314 245 19
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NcoI C'CATG_G
Cuts at: 107 107
Size: 4391
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 1444 1944 2851 1444
Size: 500 907 2984
Fragments arranged by size:
2984 907 500
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PmlI CAC'GTG
Cuts at: 224 224
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 124 156 163 195 3530 3562 124
Size: 32 7 32 3335 32 953
Fragments arranged by size:
3335 953 32 32 32 7
PstI C_TGCA'G
Cuts at: 392 1266 1438 2123 392
Size: 874 172 685 2660
Fragments arranged by size:
2660 874 685 172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 499 1816 2176 499
Size: 1317 360 2714
Fragments arranged by size:
2714 1317 360
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 1304 1405 1304
Size: 101 4290
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 703 3908 703
Size: 3205 1186
SfcI C'TryA_G
Cuts at: 33 388 1262 1434 2119 3112 3303 33
Size: 355 874 172 685 993 191 1121
Fragments arranged by size:
1121 993 874 685 355 191 172
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 1150 1546 2461 2953 3215 1150
Size: 396 915 492 262 2326
Fragments arranged by size:
2326 915 492 396 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
SspI AAT'ATT
Cuts at: 1167 1167
Size: 4391
StyI C'CwwG_G
Cuts at: 107 1215 1305 1525 1879 107
Size: 1108 90 220 354 2619
Fragments arranged by size:
2619 1108 354 220 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1974 2107 2566 2775 1974
Size: 133 459 209 3590
Fragments arranged by size:
3590 459 209 133
TatI w'GTAC_w
Cuts at: 701 743 3906 701
Size: 42 3163 1186
Fragments arranged by size:
3163 1186 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3908 3940 3908
Size: 32 4359
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1886 120
Size: 1766 2625
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI