Restriction map for
pF1KE5572
(Circular) (Six-base) MAPSORT of: pF1KE5572.seq Check: 7455 from: 1 to: 4496
pF1KE5572 4496 bp
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 934 934
Size: 4496
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 1198 1379 2321 2753 3500 3768 743
Size: 455 181 942 432 747 268 1471
Fragments arranged by size:
1471 942 747 455 432 268 181
AflIII A'CryG_T
Cuts at: 505 1249 1286 1848 2952 3929 505
Size: 744 37 562 1104 977 1072
Fragments arranged by size:
1104 1072 977 744 562 37
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 1801 3368 1801
Size: 1567 2929
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 224 364 714 1147 1500 3849 4105 224
Size: 140 350 433 353 2349 256 615
Fragments arranged by size:
2349 615 433 353 350 256 140
AseI AT'TA_AT
Cuts at: 20 443 1034 20
Size: 423 591 3482
Fragments arranged by size:
3482 591 423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 782 1516 782
Size: 734 3762
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1430 1463 1430
Size: 33 4463
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 1510 2540 1510
Size: 1030 3466
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 1176 1246 1176
Size: 70 4426
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 1458 2394 3155 4496 1458
Size: 936 761 1341 1458
Fragments arranged by size:
1458 1341 936 761
BclI T'GATC_A
Cuts at: 125 204 2016 125
Size: 79 1812 2605
Fragments arranged by size:
2605 1812 79
BfrBI ATG'CAT
Cuts at: 856 856
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 2121 2214 3270 2121
Size: 93 1056 3347
Fragments arranged by size:
3347 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 1672 2551 3043 3341 307
Size: 1365 879 492 298 1462
Fragments arranged by size:
1462 1365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 389 2010 4047 6
Size: 383 1621 2037 455
Fragments arranged by size:
2037 1621 455 383
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 976 976
Size: 4496
BseYI C'CCAG_C
Cuts at: 1172 1397 1921 2679 3256 3752 1172
Size: 225 524 758 577 496 1916
Fragments arranged by size:
1916 758 577 524 496 225
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1510 2288 2478 3270 1510
Size: 778 190 792 2736
Fragments arranged by size:
2736 792 778 190
BsmI GAATG_Cn'
Cuts at: 856 856
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 1510 2121 2214 2288 2478 2540 3270 1510
Size: 611 93 74 190 62 730 2736
Fragments arranged by size:
2736 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 207 3672 207
Size: 3465 1031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 819 934 1546 1760 2062 2443 819
Size: 115 612 214 302 381 2872
Fragments arranged by size:
2872 612 381 302 214 115
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1052 1112 1421 2408 1052
Size: 60 309 987 3140
Fragments arranged by size:
3140 987 309 60
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BsrGI T'GTAC_A
Cuts at: 190 190
Size: 4496
BssSI C'ACGA_G
Cuts at: 329 2767 3125 329
Size: 2438 358 1700
Fragments arranged by size:
2438 1700 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 999 2857 999
Size: 1858 2638
BstYI r'GATC_y
Cuts at: 1 1153 1521 1988 2011 2346 2592 3604 3690
Size: 1152 368 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1152 1012 467 449 368 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 632 1739 632
Size: 1107 3389
DraI TTT'AAA
Cuts at: 416 1495 416
Size: 1079 3417
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 235 2081 2255 2646 2673 4018 235
Size: 1846 174 391 27 1345 713
Fragments arranged by size:
1846 1345 713 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 260 639 1173 1400 2519 2729 260
Size: 379 534 227 1119 210 2027
Fragments arranged by size:
2027 1119 534 379 227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 1198 1379 2321 2753 3500 3768 743
Size: 455 181 942 432 747 268 1471
Fragments arranged by size:
1471 942 747 455 432 268 181
EcoO109I rG'GnC_Cy
Cuts at: 1345 1635 1345
Size: 290 4206
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
EcoRV GAT'ATC
Cuts at: 172 589 172
Size: 417 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 301 2515 301
Size: 2214 2282
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3200 4179 1930
Size: 248 1022 979 2247
Fragments arranged by size:
2247 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 857 889 2338 2368 2370 2400 161
Size: 32 664 32 1449 30 2 30 2257
Fragments arranged by size:
2257 1449 664 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1239 1461 1535 1239
Size: 222 74 4200
Fragments arranged by size:
4200 222 74
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 127 636 2485 2612 127
Size: 509 1849 127 2011
Fragments arranged by size:
2011 1849 509 127
MspA1I CmG'CkG
Cuts at: 1607 1921 2281 3294 3539 1607
Size: 314 360 1013 245 2564
Fragments arranged by size:
2564 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NsiI A_TGCA'T
Cuts at: 858 858
Size: 4496
NspI r_CATG'y
Cuts at: 509 1253 1290 1549 2049 2956 509
Size: 744 37 259 500 907 2049
Fragments arranged by size:
2049 907 744 500 259 37
PciI A'CATG_T
Cuts at: 505 1249 1286 2952 505
Size: 744 37 1666 2049
Fragments arranged by size:
2049 1666 744 37
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 1345 1345
Size: 4496
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SanDI GG'GwC_CC
Cuts at: 1345 1345
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SexAI A'CCwGG_T
Cuts at: 810 810
Size: 4496
SfcI C'TryA_G
Cuts at: 33 1539 2224 3217 3408 33
Size: 1506 685 993 191 1121
Fragments arranged by size:
1506 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 286 1651 2566 3058 3320 286
Size: 1365 915 492 262 1462
Fragments arranged by size:
1462 1365 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
SspI AAT'ATT
Cuts at: 957 957
Size: 4496
StuI AGG'CCT
Cuts at: 146 146
Size: 4496
StyI C'CwwG_G
Cuts at: 823 1442 1630 1984 823
Size: 619 188 354 3335
Fragments arranged by size:
3335 619 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 775 1256 2079 2212 2671 2880 775
Size: 481 823 133 459 209 2391
Fragments arranged by size:
2391 823 481 459 209 133
TatI w'GTAC_w
Cuts at: 190 577 1040 4011 190
Size: 387 463 2971 675
Fragments arranged by size:
2971 675 463 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 4013 4045 295
Size: 32 3686 32 746
Fragments arranged by size:
3686 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 648 1138 648
Size: 490 4006
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BglI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI