Restriction map for
pF1KE5724
(Circular) (Six-base) MAPSORT of: pF1KE5724.seq Check: 2159 from: 1 to: 5435
pF1KE5724 5435 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1194 2139 1194
Size: 945 4490
AatII G_ACGT'C
Cuts at: 1082 1082
Size: 5435
AccI GT'mk_AC
Cuts at: 707 2473 707
Size: 1766 3669
Acc65I G'GTAC_C
Cuts at: 2451 2451
Size: 5435
AclI AA'CG_TT
Cuts at: 5275 5275
Size: 5435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 3260 3692 4439 4707 1924
Size: 1336 432 747 268 2652
Fragments arranged by size:
2652 1336 747 432 268
AfeI AGC'GCT
Cuts at: 1780 1780
Size: 5435
AflIII A'CryG_T
Cuts at: 1217 2787 3891 4868 1217
Size: 1570 1104 977 1784
Fragments arranged by size:
1784 1570 1104 977
AgeI A'CCGG_T
Cuts at: 3823 3823
Size: 5435
AlwNI CAG_nnn'CTG
Cuts at: 918 1164 1951 2386 2740 4307 918
Size: 246 787 435 354 1567 2046
Fragments arranged by size:
2046 1567 787 435 354 246
ApaI G_GGCC'C
Cuts at: 726 1875 1996 726
Size: 1149 121 4165
Fragments arranged by size:
4165 1149 121
ApaLI G'TGCA_C
Cuts at: 4205 4205
Size: 5435
ApoI r'AATT_y
Cuts at: 1758 2439 4788 5044 1758
Size: 681 2349 256 2149
Fragments arranged by size:
2349 2149 681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
AvaI C'yCGr_G
Cuts at: 1547 1853 1875 2159 2228 2402 2455 1547
Size: 306 22 284 69 174 53 4527
Fragments arranged by size:
4527 306 284 174 69 53 22
BamHI G'GATC_C
Cuts at: 1570 2460 1570
Size: 890 4545
BanI G'GyrC_C
Cuts at: 171 248 585 921 1059 1111 1543 2079 2451
Size: 77 337 336 138 52 432 536 372
Cuts at: 2451 2865 3113 3148 171
Size: 414 248 35 2458
Fragments arranged by size:
2458 536 432 414 372 337 336 248
138 77 52 35
BanII G_rGCy'C
Cuts at: 726 1486 1875 1890 1996 2339 2449 3479 726
Size: 760 389 15 106 343 110 1030 2682
Fragments arranged by size:
2682 1030 760 389 343 110 106 15
BbeI G_GCGC'C
Cuts at: 1547 2869 3117 1547
Size: 1322 248 3865
Fragments arranged by size:
3865 1322 248
BbsI GAAGACnn'nnnn_
Cuts at: 483 1443 483
Size: 960 4475
BbvCI CC'TCA_GC
Cuts at: 701 763 2267 701
Size: 62 1504 3869
Fragments arranged by size:
3869 1504 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1278 1312 1278
Size: 34 5401
BciVI GTATCCnnnnn_n'
Cuts at: 3333 4094 5435 3333
Size: 761 1341 3333
Fragments arranged by size:
3333 1341 761
BclI T'GATC_A
Cuts at: 401 2955 401
Size: 2554 2881
BglI GCCn_nnn'nGGC
Cuts at: 112 2373 112
Size: 2261 3174
BglII A'GATC_T
Cuts at: 815 1586 2950 4987 815
Size: 771 1364 2037 1263
Fragments arranged by size:
2037 1364 1263 771
BlpI GC'TnA_GC
Cuts at: 1096 1324 1889 2052 2547 1096
Size: 228 565 163 495 3984
Fragments arranged by size:
3984 565 495 228 163
Bme1580I G_kGCm'C
Cuts at: 726 926 1875 1996 2084 3060 3153 4209 726
Size: 200 949 121 88 976 93 1056 1952
Fragments arranged by size:
1952 1056 976 949 200 121 93 88
BmrI ACTGGGnnnn_n'
Cuts at: 402 906 2647 2728 3063 5207 402
Size: 504 1741 81 335 2144 630
Fragments arranged by size:
2144 1741 630 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 489 3802 489
Size: 3313 2122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 250 703 2003 2048 2183 2395 184
Size: 66 453 1300 45 135 212 3224
Fragments arranged by size:
3224 1300 453 212 135 66 45
Bpu10I CC'TnA_GC
Cuts at: 233 701 754 763 2267 2387 233
Size: 468 53 9 1504 120 3281
Fragments arranged by size:
3281 1504 468 120 53 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2611 3490 3982 4280 1705
Size: 906 879 492 298 2860
Fragments arranged by size:
2860 906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 963 2184 2413 5139 963
Size: 1221 229 2726 1259
Fragments arranged by size:
2726 1259 1221 229
BsaAI yAC'GTr
Cuts at: 2788 2788
Size: 5435
BsaBI GATnn'nnATC
Cuts at: 6 2949 4986 6
Size: 2943 2037 455
Fragments arranged by size:
2943 2037 455
BsaHI Gr'CG_yC
Cuts at: 1079 1179 1544 2866 3114 3816 5312 1079
Size: 100 365 1322 248 702 1496 1202
Fragments arranged by size:
1496 1322 1202 702 365 248 100
BsaWI w'CCGG_w
Cuts at: 383 2624 3145 3823 4097 4244 4655 383
Size: 2241 521 678 274 147 411 1163
Fragments arranged by size:
2241 1163 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 271 301 676 706 271
Size: 30 375 30 5000
Fragments arranged by size:
5000 375 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 258 481 577 826 1357 1702 2219 258
Size: 223 96 249 531 345 517 3474
Fragments arranged by size:
3474 531 517 345 249 223 96
BseYI C'CCAG_C
Cuts at: 953 1408 2187 2363 2860 3618 4195 4691 953
Size: 455 779 176 497 758 577 496 1697
Fragments arranged by size:
1697 779 758 577 497 496 455 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2128 2128
Size: 5435
BsiEI CG_ry'CG
Cuts at: 104 127 200 2977 3023 4231 104
Size: 23 73 2777 46 1208 1308
Fragments arranged by size:
2777 1308 1208 73 46 23
BsiHKAI G_wGCw'C
Cuts at: 536 1277 1882 1890 2449 3227 3417 4209 536
Size: 741 605 8 559 778 190 792 1762
Fragments arranged by size:
1762 792 778 741 605 559 190 8
Bsp1286I G_dGCh'C
Cuts at: 536 726 926 1277 1486 1875 1882 1890 1996
Size: 190 200 351 209 389 7 8 106
Cuts at: 1996 2084 2339 2449 3060 3153 3227 3417 3479
Size: 88 255 110 611 93 74 190 62
Cuts at: 3479 4209 536
Size: 730 1762
Fragments arranged by size:
1762 730 611 389 351 255 209 200
190 190 110 106 93 88 74 62
8 7
BspEI T'CCGG_A
Cuts at: 4655 4655
Size: 5435
BspHI T'CATG_A
Cuts at: 4611 4611
Size: 5435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 620 1194 2139 2485 2699 3001 3382 620
Size: 574 945 346 214 302 381 2673
Fragments arranged by size:
2673 945 574 381 346 302 214
BsrBI CCG'CTC
Cuts at: 50 336 2319 3727 3781 5265 5430 50
Size: 286 1983 1408 54 1484 165 55
Fragments arranged by size:
1983 1484 1408 286 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 3347 1970
Size: 1377 4058
BsrFI r'CCGG_y
Cuts at: 126 1607 3433 3614 3823 126
Size: 1481 1826 181 209 1738
Fragments arranged by size:
1826 1738 1481 209 181
BssSI C'ACGA_G
Cuts at: 984 1261 1468 3706 4064 984
Size: 277 207 2238 358 2355
Fragments arranged by size:
2355 2238 358 277 207
BstAPI GCAn_nnn'nTGC
Cuts at: 1521 1830 2812 1521
Size: 309 982 4144
Fragments arranged by size:
4144 982 309
BstBI TT'CG_AA
Cuts at: 3796 3796
Size: 5435
BstXI CCAn_nnnn'nTGG
Cuts at: 849 1913 849
Size: 1064 4371
BstYI r'GATC_y
Cuts at: 1 525 575 749 815 1464 1570 1586 2460
Size: 524 50 174 66 649 106 16 874
Cuts at: 2460 2927 2950 3285 3531 4543 4629 4641 4987
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4987 1
Size: 449
Fragments arranged by size:
1012 874 649 524 467 449 346 335
246 174 106 86 66 50 23 16
12
Bsu36I CC'TnA_GG
Cuts at: 207 2331 207
Size: 2124 3311
BtgI C'CryG_G
Cuts at: 107 200 589 688 1145 1487 1539 1727 2098
Size: 93 389 99 457 342 52 188 371
Cuts at: 2098 107
Size: 3444
Fragments arranged by size:
3444 457 389 371 342 188 99 93
52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2297 3385 3566 3729 2297
Size: 1088 181 163 4003
Fragments arranged by size:
4003 1088 181 163
BtsI GCAGTG_nn'
Cuts at: 323 1418 1809 2678 323
Size: 1095 391 869 3080
Fragments arranged by size:
3080 1095 869 391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2013 2048 2013
Size: 35 5400
DraI TTT'AAA
Cuts at: 2434 2434
Size: 5435
DraIII CAC_nnn'GTG
Cuts at: 975 1830 975
Size: 855 4580
DrdI GACnn_nn'nnGTC
Cuts at: 703 955 3141 3999 703
Size: 252 2186 858 2139
Fragments arranged by size:
2186 2139 858 252
EaeI y'GGCC_r
Cuts at: 124 839 1137 1724 1967 2342 3020 3194 3585
Size: 715 298 587 243 375 678 174 391
Cuts at: 3585 3612 4957 124
Size: 27 1345 602
Fragments arranged by size:
1345 715 678 602 587 391 375 298
243 174 27
EagI C'GGCC_G
Cuts at: 124 3020 124
Size: 2896 2539
EarI CTCTTCn'nnn_
Cuts at: 648 1176 3458 3668 648
Size: 528 2282 210 2415
Fragments arranged by size:
2415 2282 528 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 763 3643 3953 4099 5245 763
Size: 2880 310 146 1146 953
Fragments arranged by size:
2880 1146 953 310 146
EcoICRI GAG'CTC
Cuts at: 1888 2447 1888
Size: 559 4876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 250 703 1924 2003 2048 2183 2395 3260
Size: 66 453 1221 79 45 135 212 865
Cuts at: 3260 3692 4439 4707 184
Size: 432 747 268 912
Fragments arranged by size:
1221 912 865 747 453 432 268 212
135 79 66 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 2201 2330 1015
Size: 1186 129 4120
Fragments arranged by size:
4120 1186 129
EcoO109I rG'GnC_Cy
Cuts at: 353 396 723 1368 1872 1992 1993 2191 2224
Size: 43 327 645 504 120 1 198 33
Cuts at: 2224 2574 353
Size: 350 3214
Fragments arranged by size:
3214 645 504 350 327 198 120 43
33 1
EcoRI G'AATT_C
Cuts at: 1758 2439 1758
Size: 681 4754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1458 1521 3454 1458
Size: 63 1933 3439
Fragments arranged by size:
3439 1933 63
FspI TGC'GCA
Cuts at: 616 3216 616
Size: 2600 2835
HaeII r_GCGC'y
Cuts at: 1547 1782 2052 2172 2869 3117 4139 5118 1547
Size: 235 270 120 697 248 1022 979 1864
Fragments arranged by size:
1864 1022 979 697 270 248 235 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 3277 3307 3309 3339 625
Size: 32 2620 30 2 30 2721
Fragments arranged by size:
2721 2620 32 30 30 2
HincII GTy'rAC
Cuts at: 279 633 2474 279
Size: 354 1841 3240
Fragments arranged by size:
3240 1841 354
KasI G'GCGC_C
Cuts at: 1543 2865 3113 1543
Size: 1322 248 3865
Fragments arranged by size:
3865 1322 248
KpnI G_GTAC'C
Cuts at: 2455 2455
Size: 5435
MluI A'CGCG_T
Cuts at: 4868 4868
Size: 5435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 4106 4290 674
Size: 3432 184 1819
Fragments arranged by size:
3432 1819 184
MscI TGG'CCA
Cuts at: 841 1726 1969 2344 3196 841
Size: 885 243 375 852 3080
Fragments arranged by size:
3080 885 852 375 243
MslI CAynn'nnrTG
Cuts at: 556 874 899 1673 3424 3551 556
Size: 318 25 774 1751 127 2440
Fragments arranged by size:
2440 1751 774 318 127 25
MspA1I CmG'CkG
Cuts at: 123 202 349 591 1389 1541 1773 1948 1989
Size: 79 147 242 798 152 232 175 41
Cuts at: 1989 2076 2271 2546 2860 3220 4233 4478 123
Size: 87 195 275 314 360 1013 245 1080
Fragments arranged by size:
1080 1013 798 360 314 275 245 242
232 195 175 152 147 87 79 41
NaeI GCC'GGC
Cuts at: 128 1609 3616 128
Size: 1481 2007 1947
Fragments arranged by size:
2007 1947 1481
NarI GG'CG_CC
Cuts at: 1544 2866 3114 1544
Size: 1322 248 3865
Fragments arranged by size:
3865 1322 248
NcoI C'CATG_G
Cuts at: 107 1727 2098 107
Size: 1620 371 3444
Fragments arranged by size:
3444 1620 371
NgoMIV G'CCGG_C
Cuts at: 126 1607 3614 126
Size: 1481 2007 1947
Fragments arranged by size:
2007 1947 1481
NruI TCG'CGA
Cuts at: 1340 1340
Size: 5435
NspI r_CATG'y
Cuts at: 1035 1074 1121 1221 1386 1933 2488 2988 3895
Size: 39 47 100 165 547 555 500 907
Cuts at: 3895 1035
Size: 2575
Fragments arranged by size:
2575 907 555 547 500 165 100 47
39
PciI A'CATG_T
Cuts at: 1217 3891 1217
Size: 2674 2761
PflMI CCAn_nnn'nTGG
Cuts at: 1164 1164
Size: 5435
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 5435
PmeI GTTT'AAAC
Cuts at: 2434 2434
Size: 5435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 4574
Size: 32 5403
PpuMI rG'GwC_Cy
Cuts at: 396 2191 2224 396
Size: 1795 33 3607
Fragments arranged by size:
3607 1795 33
PspOMI G'GGCC_C
Cuts at: 722 1871 1992 722
Size: 1149 121 4165
Fragments arranged by size:
4165 1149 121
PstI C_TGCA'G
Cuts at: 914 935 1425 1772 2482 3167 914
Size: 21 490 347 710 685 3182
Fragments arranged by size:
3182 710 685 490 347 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5435
PvuII CAG'CTG
Cuts at: 1948 2860 3220 1948
Size: 912 360 4163
Fragments arranged by size:
4163 912 360
RsrII CG'GwC_CG
Cuts at: 3630 3630
Size: 5435
SacI G_AGCT'C
Cuts at: 1890 2449 1890
Size: 559 4876
SacII CC_GC'GG
Cuts at: 203 592 1542 203
Size: 389 950 4096
Fragments arranged by size:
4096 950 389
SalI G'TCGA_C
Cuts at: 2472 2472
Size: 5435
SanDI GG'GwC_CC
Cuts at: 2191 2191
Size: 5435
SapI GCTCTTCn'nnn_
Cuts at: 3458 3668 3458
Size: 210 5225
SbfI CC_TGCA'GG
Cuts at: 2482 2482
Size: 5435
ScaI AGT'ACT
Cuts at: 1267 1910 4952 1267
Size: 643 3042 1750
Fragments arranged by size:
3042 1750 643
SexAI A'CCwGG_T
Cuts at: 619 619
Size: 5435
SfcI C'TryA_G
Cuts at: 33 372 910 931 1064 1421 1768 2478 3163
Size: 339 538 21 133 357 347 710 685
Cuts at: 3163 4156 4347 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 710 685 538 357 347 339
191 133 21
SfoI GGC'GCC
Cuts at: 1545 2867 3115 1545
Size: 1322 248 3865
Fragments arranged by size:
3865 1322 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
SmaI CCC'GGG
Cuts at: 1549 2161 2404 2457 1549
Size: 612 243 53 4527
Fragments arranged by size:
4527 612 243 53
SmlI C'TyrA_G
Cuts at: 1684 2590 3505 3997 4259 1684
Size: 906 915 492 262 2860
Fragments arranged by size:
2860 915 906 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5435
SphI G_CATG'C
Cuts at: 1074 2488 2988 1074
Size: 1414 500 3521
Fragments arranged by size:
3521 1414 500
StyI C'CwwG_G
Cuts at: 107 252 481 1727 2098 2353 2569 2923 107
Size: 145 229 1246 371 255 216 354 2619
Fragments arranged by size:
2619 1246 371 354 255 229 216 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3018 3151 3610 3819 3018
Size: 133 459 209 4634
Fragments arranged by size:
4634 459 209 133
TatI w'GTAC_w
Cuts at: 1196 1214 1265 1502 1908 4950 1196
Size: 18 51 237 406 3042 1681
Fragments arranged by size:
3042 1681 406 237 51 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4952 4984 4952
Size: 32 5403
Tth111I GACn'n_nGTC
Cuts at: 3232 3232
Size: 5435
XbaI T'CTAG_A
Cuts at: 2466 2466
Size: 5435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 969 2930 969
Size: 1961 3474
XmaI C'CCGG_G
Cuts at: 1547 2159 2402 2455 1547
Size: 612 243 53 4527
Fragments arranged by size:
4527 612 243 53
ZraI GAC'GTC
Cuts at: 1080 1080
Size: 5435
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI