Restriction map for
pF1KE5778
(Circular) (Six-base) MAPSORT of: pF1KE5778.seq Check: 2054 from: 1 to: 4988
pF1KE5778 4988 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 199 1302 199
Size: 1103 3885
AatII G_ACGT'C
Cuts at: 539 539
Size: 4988
AccI GT'mk_AC
Cuts at: 603 945 1439 2026 603
Size: 342 494 587 3565
Fragments arranged by size:
3565 587 494 342
Acc65I G'GTAC_C
Cuts at: 2004 2004
Size: 4988
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 628 2813 3245 3992 4260 134
Size: 494 2185 432 747 268 862
Fragments arranged by size:
2185 862 747 494 432 268
AfeI AGC'GCT
Cuts at: 432 432
Size: 4988
AflIII A'CryG_T
Cuts at: 2340 3444 4421 2340
Size: 1104 977 2907
Fragments arranged by size:
2907 1104 977
AgeI A'CCGG_T
Cuts at: 3376 3376
Size: 4988
AleI CACnn'nnGTG
Cuts at: 1352 1936 1352
Size: 584 4404
AlwNI CAG_nnn'CTG
Cuts at: 157 1065 2293 3860 157
Size: 908 1228 1567 1285
Fragments arranged by size:
1567 1285 1228 908
ApaLI G'TGCA_C
Cuts at: 686 1068 3758 686
Size: 382 2690 1916
Fragments arranged by size:
2690 1916 382
ApoI r'AATT_y
Cuts at: 1992 4341 4597 1992
Size: 2349 256 2383
Fragments arranged by size:
2383 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4988
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 2008 2008
Size: 4988
BamHI G'GATC_C
Cuts at: 312 2013 312
Size: 1701 3287
BanI G'GyrC_C
Cuts at: 2004 2418 2666 2701 2004
Size: 414 248 35 4291
Fragments arranged by size:
4291 414 248 35
BanII G_rGCy'C
Cuts at: 2002 3032 2002
Size: 1030 3958
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4740
BbsI GAAGACnn'nnnn_
Cuts at: 213 213
Size: 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1586 1620 1885 1919 1586
Size: 34 265 34 4655
Fragments arranged by size:
4655 265 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1204 2886 3647 4988 1204
Size: 1682 761 1341 1204
Fragments arranged by size:
1682 1341 1204 761
BclI T'GATC_A
Cuts at: 1515 2508 1515
Size: 993 3995
BglII A'GATC_T
Cuts at: 2503 4540 2503
Size: 2037 2951
BlpI GC'TnA_GC
Cuts at: 2100 2100
Size: 4988
Bme1580I G_kGCm'C
Cuts at: 690 1072 2613 2706 3762 690
Size: 382 1541 93 1056 1916
Fragments arranged by size:
1916 1541 1056 382 93
BmrI ACTGGGnnnn_n'
Cuts at: 896 964 2200 2281 2616 4760 896
Size: 68 1236 81 335 2144 1124
Fragments arranged by size:
2144 1236 1124 335 81 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 944 3355 944
Size: 2411 2577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1687 1984 604
Size: 1083 297 3608
Fragments arranged by size:
3608 1083 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1958 2164 3043 3535 3833 491
Size: 1467 206 879 492 298 1646
Fragments arranged by size:
1646 1467 879 492 298 206
BsaI GGTCTCn'nnnn_
Cuts at: 4692 4692
Size: 4988
BsaAI yAC'GTr
Cuts at: 557 1950 2341 557
Size: 1393 391 3204
Fragments arranged by size:
3204 1393 391
BsaBI GATnn'nnATC
Cuts at: 6 2502 4539 6
Size: 2496 2037 455
Fragments arranged by size:
2496 2037 455
BsaHI Gr'CG_yC
Cuts at: 536 2419 2667 3369 4865 536
Size: 1883 248 702 1496 659
Fragments arranged by size:
1883 1496 702 659 248
BsaWI w'CCGG_w
Cuts at: 2177 2698 3376 3650 3797 4208 2177
Size: 521 678 274 147 411 2957
Fragments arranged by size:
2957 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1211 1211
Size: 4988
BseYI C'CCAG_C
Cuts at: 577 1107 1649 1889 2413 3171 3748 4244 577
Size: 530 542 240 524 758 577 496 1321
Fragments arranged by size:
1321 758 577 542 530 524 496 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 780 1306 240
Size: 540 526 3922
Fragments arranged by size:
3922 540 526
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3784 104
Size: 2426 46 1208 1308
Fragments arranged by size:
2426 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 507 690 1072 1908 2002 2780 2970 3762 507
Size: 183 382 836 94 778 190 792 1733
Fragments arranged by size:
1733 836 792 778 382 190 183 94
BsiWI C'GTAC_G
Cuts at: 557 557
Size: 4988
BsmI GAATG_Cn'
Cuts at: 517 517
Size: 4988
Bsp1286I G_dGCh'C
Cuts at: 507 690 1072 1908 2002 2613 2706 2780 2970
Size: 183 382 836 94 611 93 74 190
Cuts at: 2970 3032 3762 507
Size: 62 730 1733
Fragments arranged by size:
1733 836 730 611 382 190 183 94
93 74 62
BspEI T'CCGG_A
Cuts at: 4208 4208
Size: 4988
BspHI T'CATG_A
Cuts at: 4164 4164
Size: 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 199 1302 2038 2252 2554 2935 199
Size: 1103 736 214 302 381 2252
Fragments arranged by size:
2252 1103 736 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1396 1917 3280 3334 4818 4983 50
Size: 1346 521 1363 54 1484 165 55
Fragments arranged by size:
1484 1363 1346 521 165 55 54
BsrDI GCAATG_nn'
Cuts at: 418 2900 418
Size: 2482 2506
BsrFI r'CCGG_y
Cuts at: 1789 2986 3167 3376 1789
Size: 1197 181 209 3401
Fragments arranged by size:
3401 1197 209 181
BssSI C'ACGA_G
Cuts at: 1048 1884 3259 3617 1048
Size: 836 1375 358 2419
Fragments arranged by size:
2419 1375 836 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2365 2365
Size: 4988
BstBI TT'CG_AA
Cuts at: 3349 3349
Size: 4988
BstEII G'GTnAC_C
Cuts at: 1314 1314
Size: 4988
BstYI r'GATC_y
Cuts at: 1 312 1168 2013 2480 2503 2838 3084 4096
Size: 311 856 845 467 23 335 246 1012
Cuts at: 4096 4182 4194 4540 1
Size: 86 12 346 449
Fragments arranged by size:
1012 856 845 467 449 346 335 311
246 86 23 12
BtgI C'CryG_G
Cuts at: 1306 1306
Size: 4988
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2938 3119 3282 2938
Size: 181 163 4644
Fragments arranged by size:
4644 181 163
BtsI GCAGTG_nn'
Cuts at: 361 540 1985 2231 361
Size: 179 1445 246 3118
Fragments arranged by size:
3118 1445 246 179
DraI TTT'AAA
Cuts at: 1454 1987 1454
Size: 533 4455
DrdI GACnn_nn'nnGTC
Cuts at: 2694 3552 2694
Size: 858 4130
EaeI y'GGCC_r
Cuts at: 786 1303 1918 2573 2747 3138 3165 4510 786
Size: 517 615 655 174 391 27 1345 1264
Fragments arranged by size:
1345 1264 655 615 517 391 174 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4988
EarI CTCTTCn'nnn_
Cuts at: 286 1730 1741 3011 3221 286
Size: 1444 11 1270 210 2053
Fragments arranged by size:
2053 1444 1270 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 310 3196 3506 3652 4798 310
Size: 2886 310 146 1146 500
Fragments arranged by size:
2886 1146 500 310 146
EcoICRI GAG'CTC
Cuts at: 2000 2000
Size: 4988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 604 628 1687 1984 2813 3245 3992 4260
Size: 470 24 1059 297 829 432 747 268
Cuts at: 4260 134
Size: 862
Fragments arranged by size:
1059 862 829 747 470 432 297 268
24
EcoNI CCTnn'n_nnAGG
Cuts at: 1616 1616
Size: 4988
EcoO109I rG'GnC_Cy
Cuts at: 1837 2127 1837
Size: 290 4698
EcoRI G'AATT_C
Cuts at: 1992 1992
Size: 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1164 1737 3007 1164
Size: 573 1270 3145
Fragments arranged by size:
3145 1270 573
FspI TGC'GCA
Cuts at: 2769 2769
Size: 4988
HaeII r_GCGC'y
Cuts at: 236 434 2422 2670 3692 4671 236
Size: 198 1988 248 1022 979 553
Fragments arranged by size:
1988 1022 979 553 248 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 263 295 1187 1219 1349 1381 1465 1497 2830
Size: 32 892 32 130 32 84 32 1333
Cuts at: 2830 2860 2862 2892 263
Size: 30 2 30 2359
Fragments arranged by size:
2359 1333 892 130 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2027 2027
Size: 4988
HindIII A'AGCT_T
Cuts at: 1153 1153
Size: 4988
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4740
KpnI G_GTAC'C
Cuts at: 2008 2008
Size: 4988
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1198 1254 3659 3843 1198
Size: 56 2405 184 2343
Fragments arranged by size:
2405 2343 184 56
MscI TGG'CCA
Cuts at: 2749 2749
Size: 4988
MslI CAynn'nnrTG
Cuts at: 451 970 1352 1936 2977 3104 451
Size: 519 382 584 1041 127 2335
Fragments arranged by size:
2335 1041 584 519 382 127
MspA1I CmG'CkG
Cuts at: 1085 2099 2413 2773 3786 4031 1085
Size: 1014 314 360 1013 245 2042
Fragments arranged by size:
2042 1014 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1791 3169 1791
Size: 1378 3610
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4740
NdeI CA'TA_TG
Cuts at: 1603 1603
Size: 4988
NgoMIV G'CCGG_C
Cuts at: 1789 3167 1789
Size: 1378 3610
NspI r_CATG'y
Cuts at: 2041 2541 3448 2041
Size: 500 907 3581
Fragments arranged by size:
3581 907 500
PciI A'CATG_T
Cuts at: 3444 3444
Size: 4988
PflMI CCAn_nnn'nTGG
Cuts at: 323 323
Size: 4988
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4956
PstI C_TGCA'G
Cuts at: 958 2035 2720 958
Size: 1077 685 3226
Fragments arranged by size:
3226 1077 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 2413 2773 2413
Size: 360 4628
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 2002 2002
Size: 4988
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 3011 3221 3011
Size: 210 4778
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4988
ScaI AGT'ACT
Cuts at: 241 4505 241
Size: 4264 724
SfcI C'TryA_G
Cuts at: 33 954 1440 1794 2031 2716 3709 3900 33
Size: 921 486 354 237 685 993 191 1121
Fragments arranged by size:
1121 993 921 685 486 354 237 191
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4740
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 2010 2010
Size: 4988
SmlI C'TyrA_G
Cuts at: 506 1973 2143 3058 3550 3812 506
Size: 1467 170 915 492 262 1682
Fragments arranged by size:
1682 1467 915 492 262 170
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4988
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4488
StuI AGG'CCT
Cuts at: 1054 1054
Size: 4988
StyI C'CwwG_G
Cuts at: 2122 2476 2122
Size: 354 4634
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 281 631 1350 1641 2571 2704 3163 3372 281
Size: 350 719 291 930 133 459 209 1897
Fragments arranged by size:
1897 930 719 459 350 291 209 133
TatI w'GTAC_w
Cuts at: 239 904 4503 239
Size: 665 3599 724
Fragments arranged by size:
3599 724 665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 789 821 1228 1260 4505 4537 789
Size: 32 407 32 3245 32 1240
Fragments arranged by size:
3245 1240 407 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 757 2785 757
Size: 2028 2960
XbaI T'CTAG_A
Cuts at: 2019 2019
Size: 4988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 4988
XmaI C'CCGG_G
Cuts at: 2008 2008
Size: 4988
ZraI GAC'GTC
Cuts at: 537 537
Size: 4988
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI