Restriction map for
pF1KE5838
(Circular) (Six-base) MAPSORT of: pF1KE5838.seq Check: 6882 from: 1 to: 8234
pF1KE5838 8234 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4051 4051
Size: 8234
AccI GT'mk_AC
Cuts at: 1508 5272 1508
Size: 3764 4470
Acc65I G'GTAC_C
Cuts at: 5250 5250
Size: 8234
AclI AA'CG_TT
Cuts at: 5228 8074 5228
Size: 2846 5388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 409 2308 2985 3543 3699 3755 4511 6059
Size: 108 1899 677 558 156 56 756 1548
Cuts at: 6059 6491 7238 7506 301
Size: 432 747 268 1029
Fragments arranged by size:
1899 1548 1029 756 747 677 558 432
268 156 108 56
AfeI AGC'GCT
Cuts at: 1840 1840
Size: 8234
AflIII A'CryG_T
Cuts at: 2357 2384 3788 5586 6690 7667 2357
Size: 27 1404 1798 1104 977 2924
Fragments arranged by size:
2924 1798 1404 1104 977 27
AgeI A'CCGG_T
Cuts at: 2666 6622 2666
Size: 3956 4278
AhdI GACnn_n'nnGTC
Cuts at: 468 5169 468
Size: 4701 3533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2386 2386
Size: 8234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3718 3750 3718
Size: 32 8202
AlwNI CAG_nnn'CTG
Cuts at: 1338 2184 2373 3588 4255 5539 7106 1338
Size: 846 189 1215 667 1284 1567 2466
Fragments arranged by size:
2466 1567 1284 1215 846 667 189
ApaI G_GGCC'C
Cuts at: 1700 3094 1700
Size: 1394 6840
ApaLI G'TGCA_C
Cuts at: 7004 7004
Size: 8234
ApoI r'AATT_y
Cuts at: 180 2441 2698 5238 7587 7843 180
Size: 2261 257 2540 2349 256 571
Fragments arranged by size:
2540 2349 2261 571 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8234
AvaI C'yCGr_G
Cuts at: 833 3317 3954 5254 833
Size: 2484 637 1300 3813
Fragments arranged by size:
3813 2484 1300 637
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4394 4427 4394
Size: 33 8201
BamHI G'GATC_C
Cuts at: 5259 5259
Size: 8234
BanI G'GyrC_C
Cuts at: 4716 5250 5664 5912 5947 4716
Size: 534 414 248 35 7003
Fragments arranged by size:
7003 534 414 248 35
BanII G_rGCy'C
Cuts at: 728 1700 2024 2228 2594 3094 3671 4337 5248
Size: 972 324 204 366 500 577 666 911
Cuts at: 5248 6278 728
Size: 1030 2684
Fragments arranged by size:
2684 1030 972 911 666 577 500 366
324 204
BbeI G_GCGC'C
Cuts at: 5668 5916 5668
Size: 248 7986
BbsI GAAGACnn'nnnn_
Cuts at: 1044 1309 3884 4296 1044
Size: 265 2575 412 4982
Fragments arranged by size:
4982 2575 412 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 8200
BciVI GTATCCnnnnn_n'
Cuts at: 2746 3752 6132 6893 8234 2746
Size: 1006 2380 761 1341 2746
Fragments arranged by size:
2746 2380 1341 1006 761
BclI T'GATC_A
Cuts at: 3551 5754 3551
Size: 2203 6031
BfrBI ATG'CAT
Cuts at: 972 3701 972
Size: 2729 5505
BglI GCCn_nnn'nGGC
Cuts at: 2110 3385 2110
Size: 1275 6959
BglII A'GATC_T
Cuts at: 1526 4516 5749 7786 1526
Size: 2990 1233 2037 1974
Fragments arranged by size:
2990 2037 1974 1233
BlpI GC'TnA_GC
Cuts at: 1331 1485 5346 1331
Size: 154 3861 4219
Fragments arranged by size:
4219 3861 154
Bme1580I G_kGCm'C
Cuts at: 1700 3094 5859 5952 7008 1700
Size: 1394 2765 93 1056 2926
Fragments arranged by size:
2926 2765 1394 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 404 5446 5527 5862 8006 404
Size: 5042 81 335 2144 632
Fragments arranged by size:
5042 2144 632 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6601 6601
Size: 8234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 770 788 1048 1585 1647 2316 2613 3823
Size: 94 18 260 537 62 669 297 1210
Cuts at: 3823 3829 3928 3946 4391 4835 676
Size: 6 99 18 445 444 4075
Fragments arranged by size:
4075 1210 669 537 445 444 297 260
99 94 62 18 18 6
Bpu10I CC'TnA_GC
Cuts at: 149 4192 149
Size: 4043 4191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1944 2410 3655 4674 4769 5410 6289 6781
Size: 1256 466 1245 1019 95 641 879 492
Cuts at: 6781 7079 688
Size: 298 1843
Fragments arranged by size:
1843 1256 1245 1019 879 641 492 466
298 95
BsaI GGTCTCn'nnnn_
Cuts at: 3307 4063 7938 3307
Size: 756 3875 3603
Fragments arranged by size:
3875 3603 756
BsaAI yAC'GTr
Cuts at: 2309 2360 5587 2309
Size: 51 3227 4956
Fragments arranged by size:
4956 3227 51
BsaBI GATnn'nnATC
Cuts at: 6 1777 2824 5748 7785 6
Size: 1771 1047 2924 2037 455
Fragments arranged by size:
2924 2037 1771 1047 455
BsaHI Gr'CG_yC
Cuts at: 378 3980 5665 5913 6615 8111 378
Size: 3602 1685 248 702 1496 501
Fragments arranged by size:
3602 1685 1496 702 501 248
BsaWI w'CCGG_w
Cuts at: 236 1844 2666 5423 5944 6622 6896 7043 7454
Size: 1608 822 2757 521 678 274 147 411
Cuts at: 7454 236
Size: 1016
Fragments arranged by size:
2757 1608 1016 822 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 587 617 1010 1040 3775 3805 4060 4090 4486
Size: 30 393 30 2735 30 255 30 396
Cuts at: 4486 4516 4817 4847 587
Size: 30 301 30 3974
Fragments arranged by size:
3974 2735 396 393 301 255 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 203 595 2101 3223 3418 4081 4915 5044 5193
Size: 392 1506 1122 195 663 834 129 149
Cuts at: 5193 203
Size: 3244
Fragments arranged by size:
3244 1506 1122 834 663 392 195 149
129
BseYI C'CCAG_C
Cuts at: 343 1899 4383 4885 5659 6417 6994 7490 343
Size: 1556 2484 502 774 758 577 496 1087
Fragments arranged by size:
2484 1556 1087 774 758 577 502 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2382 3957 2382
Size: 1575 6659
BsiEI CG_ry'CG
Cuts at: 104 5776 5822 7030 104
Size: 5672 46 1208 1308
Fragments arranged by size:
5672 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2024 2228 2594 3671 3827 4337 5248 6026 6216
Size: 204 366 1077 156 510 911 778 190
Cuts at: 6216 7008 2024
Size: 792 3250
Fragments arranged by size:
3250 1077 911 792 778 510 366 204
190 156
BsmI GAATG_Cn'
Cuts at: 972 4555 972
Size: 3583 4651
BsmBI CGTCTCn'nnnn_
Cuts at: 2989 3061 2989
Size: 72 8162
Bsp1286I G_dGCh'C
Cuts at: 728 1700 2024 2228 2594 3094 3671 3827 4337
Size: 972 324 204 366 500 577 156 510
Cuts at: 4337 5248 5859 5952 6026 6216 6278 7008 728
Size: 911 611 93 74 190 62 730 1954
Fragments arranged by size:
1954 972 911 730 611 577 510 500
366 324 204 190 156 93 74 62
BspEI T'CCGG_A
Cuts at: 236 1844 7454 236
Size: 1608 5610 1016
Fragments arranged by size:
5610 1608 1016
BspHI T'CATG_A
Cuts at: 194 2042 2139 3512 7410 194
Size: 1848 97 1373 3898 1018
Fragments arranged by size:
3898 1848 1373 1018 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1754 4051 5284 5498 5800 6181 1754
Size: 2297 1233 214 302 381 3807
Fragments arranged by size:
3807 2297 1233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3930 3953 6526 6580 8064 8229 50
Size: 3880 23 2573 54 1484 165 55
Fragments arranged by size:
3880 2573 1484 165 55 54 23
BsrDI GCAATG_nn'
Cuts at: 143 746 2606 3431 3703 4931 6146 143
Size: 603 1860 825 272 1228 1215 2231
Fragments arranged by size:
2231 1860 1228 1215 825 603 272
BsrFI r'CCGG_y
Cuts at: 1551 2396 2666 3375 6232 6413 6622 1551
Size: 845 270 709 2857 181 209 3163
Fragments arranged by size:
3163 2857 845 709 270 209 181
BsrGI T'GTAC_A
Cuts at: 1679 1679
Size: 8234
BssHII G'CGCG_C
Cuts at: 5007 5007
Size: 8234
BssSI C'ACGA_G
Cuts at: 3610 6505 6863 3610
Size: 2895 358 4981
Fragments arranged by size:
4981 2895 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2123 4255 4450 5611 2123
Size: 2132 195 1161 4746
Fragments arranged by size:
4746 2132 1161 195
BstBI TT'CG_AA
Cuts at: 1035 6595 1035
Size: 5560 2674
BstXI CCAn_nnnn'nTGG
Cuts at: 119 397 547 2106 3906 4266 119
Size: 278 150 1559 1800 360 4087
Fragments arranged by size:
4087 1800 1559 360 278 150
BstYI r'GATC_y
Cuts at: 1 1526 1558 4516 5259 5726 5749 6084 6330
Size: 1525 32 2958 743 467 23 335 246
Cuts at: 6330 7342 7428 7440 7786 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2958 1525 1012 743 467 449 346 335
246 86 32 23 12
BtgI C'CryG_G
Cuts at: 118 666 2093 2132 2324 3851 3899 4505 118
Size: 548 1427 39 192 1527 48 606 3847
Fragments arranged by size:
3847 1527 1427 606 548 192 48 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 818 1497 2334 2931 6184 6365 6528 818
Size: 679 837 597 3253 181 163 2524
Fragments arranged by size:
3253 2524 837 679 597 181 163
BtsI GCAGTG_nn'
Cuts at: 5477 5477
Size: 8234
ClaI AT'CG_AT
Cuts at: 3469 3469
Size: 8234
DraI TTT'AAA
Cuts at: 4179 5233 4179
Size: 1054 7180
DraIII CAC_nnn'GTG
Cuts at: 4769 4769
Size: 8234
DrdI GACnn_nn'nnGTC
Cuts at: 3016 4315 5940 6798 3016
Size: 1299 1625 858 4452
Fragments arranged by size:
4452 1625 1299 858
EaeI y'GGCC_r
Cuts at: 549 916 1549 2096 2208 2261 2800 3227 3377
Size: 367 633 547 112 53 539 427 150
Cuts at: 3377 3896 4268 5819 5993 6384 6411 7756 549
Size: 519 372 1551 174 391 27 1345 1027
Fragments arranged by size:
1551 1345 1027 633 547 539 519 427
391 372 367 174 150 112 53 27
EagI C'GGCC_G
Cuts at: 5819 5819
Size: 8234
EarI CTCTTCn'nnn_
Cuts at: 897 1870 1955 2573 2639 2770 3655 3686 4640
Size: 973 85 618 66 131 885 31 954
Cuts at: 4640 5056 6257 6467 897
Size: 416 1201 210 2664
Fragments arranged by size:
2664 1201 973 954 885 618 416 210
131 85 66 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1268 6442 6752 6898 8044 1268
Size: 5174 310 146 1146 1458
Fragments arranged by size:
5174 1458 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2022 2226 2592 3669 4335 5246 2022
Size: 204 366 1077 666 911 5010
Fragments arranged by size:
5010 1077 911 666 366 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 409 676 770 788 1048 1585 1647 2308
Size: 108 267 94 18 260 537 62 661
Cuts at: 2308 2316 2613 2985 3543 3699 3755 3823 3829
Size: 8 297 372 558 156 56 68 6
Cuts at: 3829 3928 3946 4391 4511 4835 6059 6491 7238
Size: 99 18 445 120 324 1224 432 747
Cuts at: 7238 7506 301
Size: 268 1029
Fragments arranged by size:
1224 1029 747 661 558 537 445 432
372 324 297 268 267 260 156 120
108 99 94 68 62 56 18 18
8 6
EcoO109I rG'GnC_Cy
Cuts at: 672 891 1126 1697 2103 3959 4187 5373 672
Size: 219 235 571 406 1856 228 1186 3533
Fragments arranged by size:
3533 1856 1186 571 406 235 228 219
EcoRI G'AATT_C
Cuts at: 2441 2698 5238 2441
Size: 257 2540 5437
Fragments arranged by size:
5437 2540 257
EcoRV GAT'ATC
Cuts at: 3473 3473
Size: 8234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 729 3211 3255 4136 6253 729
Size: 2482 44 881 2117 2710
Fragments arranged by size:
2710 2482 2117 881 44
FspI TGC'GCA
Cuts at: 2410 6015 2410
Size: 3605 4629
HaeII r_GCGC'y
Cuts at: 1842 3915 5668 5916 6938 7917 1842
Size: 2073 1753 248 1022 979 2159
Fragments arranged by size:
2159 2073 1753 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 454 486 808 840 896 928 1496
Size: 32 108 32 322 32 56 32 568
Cuts at: 1496 1528 2967 2999 3199 3231 3279 3311 3353
Size: 32 1439 32 200 32 48 32 42
Cuts at: 3353 3385 3775 3807 4060 4092 4486 4501 4518
Size: 32 390 32 253 32 394 15 17
Cuts at: 4518 4533 5155 5187 6076 6106 6108 6138 314
Size: 15 622 32 889 30 2 30 2410
Fragments arranged by size:
2410 1439 889 622 568 394 390 322
253 200 108 56 48 42 32 32
32 32 32 32 32 32 32 32
32 32 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 777 1509 2166 3423 3813 5273 777
Size: 732 657 1257 390 1460 3738
Fragments arranged by size:
3738 1460 1257 732 657 390
HindIII A'AGCT_T
Cuts at: 649 4395 5208 649
Size: 3746 813 3675
Fragments arranged by size:
3746 3675 813
HpaI GTT'AAC
Cuts at: 777 777
Size: 8234
KasI G'GCGC_C
Cuts at: 5664 5912 5664
Size: 248 7986
KpnI G_GTAC'C
Cuts at: 5254 5254
Size: 8234
MfeI C'AATT_G
Cuts at: 4790 4790
Size: 8234
MluI A'CGCG_T
Cuts at: 7667 7667
Size: 8234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 581 617 1559 2949 3500 3965 4114 4145 5003
Size: 36 942 1390 551 465 149 31 858
Cuts at: 5003 6905 7089 581
Size: 1902 184 1726
Fragments arranged by size:
1902 1726 1390 942 858 551 465 184
149 36 31
MscI TGG'CCA
Cuts at: 551 2098 2210 2263 3229 3898 4270 5995 551
Size: 1547 112 53 966 669 372 1725 2790
Fragments arranged by size:
2790 1725 1547 966 669 372 112 53
MslI CAynn'nnrTG
Cuts at: 571 1351 2383 2389 2599 3148 3571 3793 4299
Size: 780 1032 6 210 549 423 222 506
Cuts at: 4299 4504 6223 6350 571
Size: 205 1719 127 2455
Fragments arranged by size:
2455 1719 1032 780 549 506 423 222
210 205 127 6
MspA1I CmG'CkG
Cuts at: 1284 2214 2259 3342 3873 5097 5345 5659 6019
Size: 930 45 1083 531 1224 248 314 360
Cuts at: 6019 7032 7277 1284
Size: 1013 245 2241
Fragments arranged by size:
2241 1224 1083 1013 930 531 360 314
248 245 45
NaeI GCC'GGC
Cuts at: 2398 3377 6415 2398
Size: 979 3038 4217
Fragments arranged by size:
4217 3038 979
NarI GG'CG_CC
Cuts at: 5665 5913 5665
Size: 248 7986
NcoI C'CATG_G
Cuts at: 666 2093 2132 4505 666
Size: 1427 39 2373 4395
Fragments arranged by size:
4395 2373 1427 39
NdeI CA'TA_TG
Cuts at: 5046 5046
Size: 8234
NgoMIV G'CCGG_C
Cuts at: 2396 3375 6413 2396
Size: 979 3038 4217
Fragments arranged by size:
4217 3038 979
NsiI A_TGCA'T
Cuts at: 974 3703 974
Size: 2729 5505
NspI r_CATG'y
Cuts at: 397 972 1644 2388 2409 2496 3249 3792 5287
Size: 575 672 744 21 87 753 543 1495
Cuts at: 5287 5787 6694 397
Size: 500 907 1937
Fragments arranged by size:
1937 1495 907 753 744 672 575 543
500 87 21
PasI CC'CwG_GG
Cuts at: 866 2844 4562 866
Size: 1978 1718 4538
Fragments arranged by size:
4538 1978 1718
PciI A'CATG_T
Cuts at: 2384 3788 6690 2384
Size: 1404 2902 3928
Fragments arranged by size:
3928 2902 1404
PflMI CCAn_nnn'nTGG
Cuts at: 629 1287 3852 629
Size: 658 2565 5011
Fragments arranged by size:
5011 2565 658
PfoI T'CCnGG_A
Cuts at: 295 2228 2629 295
Size: 1933 401 5900
Fragments arranged by size:
5900 1933 401
PmeI GTTT'AAAC
Cuts at: 5233 5233
Size: 8234
PmlI CAC'GTG
Cuts at: 2309 2360 2309
Size: 51 8183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1314 1346 7373 7405 1314
Size: 32 6027 32 2143
Fragments arranged by size:
6027 2143 32 32
PpuMI rG'GwC_Cy
Cuts at: 891 1126 3959 4187 891
Size: 235 2833 228 4938
Fragments arranged by size:
4938 2833 235 228
PsiI TTA'TAA
Cuts at: 317 317
Size: 8234
PspOMI G'GGCC_C
Cuts at: 1696 3090 1696
Size: 1394 6840
PspXI vC'TCGA_Gb
Cuts at: 3954 3954
Size: 8234
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3025 3057 3226 3258 3025
Size: 32 169 32 8001
Fragments arranged by size:
8001 169 32 32
PstI C_TGCA'G
Cuts at: 680 2318 2921 4062 4251 5281 5966 680
Size: 1638 603 1141 189 1030 685 2948
Fragments arranged by size:
2948 1638 1141 1030 685 603 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8234
PvuII CAG'CTG
Cuts at: 1284 2214 2259 3342 3873 5659 6019 1284
Size: 930 45 1083 531 1786 360 3499
Fragments arranged by size:
3499 1786 1083 930 531 360 45
RsrII CG'GwC_CG
Cuts at: 6429 6429
Size: 8234
SacI G_AGCT'C
Cuts at: 2024 2228 2594 3671 4337 5248 2024
Size: 204 366 1077 666 911 5010
Fragments arranged by size:
5010 1077 911 666 366 204
SalI G'TCGA_C
Cuts at: 1507 5271 1507
Size: 3764 4470
SapI GCTCTTCn'nnn_
Cuts at: 2770 3686 5056 6257 6467 2770
Size: 916 1370 1201 210 4537
Fragments arranged by size:
4537 1370 1201 916 210
SbfI CC_TGCA'GG
Cuts at: 4062 5281 4062
Size: 1219 7015
ScaI AGT'ACT
Cuts at: 2926 7751 2926
Size: 4825 3409
SfcI C'TryA_G
Cuts at: 33 676 1758 2314 2896 2917 4058 4223 4247
Size: 643 1082 556 582 21 1141 165 24
Cuts at: 4247 5277 5962 6955 7146 33
Size: 1030 685 993 191 1121
Fragments arranged by size:
1141 1121 1082 1030 993 685 643 582
556 191 165 24 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2110 3385 2110
Size: 1275 6959
SfoI GGC'GCC
Cuts at: 5666 5914 5666
Size: 248 7986
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8234
SmaI CCC'GGG
Cuts at: 5256 5256
Size: 8234
SmlI C'TyrA_G
Cuts at: 703 833 1923 2425 3670 3954 4689 4784 5389
Size: 130 1090 502 1245 284 735 95 605
Cuts at: 5389 6304 6796 7058 703
Size: 915 492 262 1879
Fragments arranged by size:
1879 1245 1090 915 735 605 502 492
284 262 130 95
SpeI A'CTAG_T
Cuts at: 67 4210 67
Size: 4143 4091
SphI G_CATG'C
Cuts at: 972 1644 2409 5287 5787 972
Size: 672 765 2878 500 3419
Fragments arranged by size:
3419 2878 765 672 500
StuI AGG'CCT
Cuts at: 2146 2423 2146
Size: 277 7957
StyI C'CwwG_G
Cuts at: 540 623 666 2093 2099 2132 4265 4377 4505
Size: 83 43 1427 6 33 2133 112 128
Cuts at: 4505 5368 5722 540
Size: 863 354 3052
Fragments arranged by size:
3052 2133 1427 863 354 128 112 83
43 33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1075 2727 2824 4119 4470 4552 5817 5950 6409
Size: 1652 97 1295 351 82 1265 133 459
Cuts at: 6409 6618 1075
Size: 209 2691
Fragments arranged by size:
2691 1652 1295 1265 459 351 209 133
97 82
TatI w'GTAC_w
Cuts at: 1679 2924 3134 3678 7749 1679
Size: 1245 210 544 4071 2164
Fragments arranged by size:
4071 2164 1245 544 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1010 1042 1118 1150 4817 4849 7751 7783 1010
Size: 32 76 32 3667 32 2902 32 1461
Fragments arranged by size:
3667 2902 1461 76 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 930 3963 4074 6031 930
Size: 3033 111 1957 3133
Fragments arranged by size:
3133 3033 1957 111
XbaI T'CTAG_A
Cuts at: 4641 5265 4641
Size: 624 7610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2106 5729 2106
Size: 3623 4611
XhoI C'TCGA_G
Cuts at: 833 3954 833
Size: 3121 5113
XmaI C'CCGG_G
Cuts at: 5254 5254
Size: 8234
XmnI GAAnn'nnTTC
Cuts at: 1306 3238 3649 4030 4116 5065 1306
Size: 1932 411 381 86 949 4475
Fragments arranged by size:
4475 1932 949 411 381 86
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AscI AvrII BbvCI BmgBI BmtI
BsiWI BstEII BstZ17I Bsu36I CspCI EcoNI FseI FspAI
NheI NotI NruI PacI PshAI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI ZraI