Restriction map for
pF1KE5841
(Circular) (Six-base) MAPSORT of: pF1KE5841.seq Check: 6171 from: 1 to: 8507
pF1KE5841 8507 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 359 1758 1827 3433 5038 5065 5466 5503 359
Size: 1399 69 1606 1605 27 401 37 3363
Fragments arranged by size:
3363 1606 1605 1399 401 69 37 27
AccI GT'mk_AC
Cuts at: 861 1193 5545 861
Size: 332 4352 3823
Fragments arranged by size:
4352 3823 332
Acc65I G'GTAC_C
Cuts at: 5523 5523
Size: 8507
AclI AA'CG_TT
Cuts at: 8347 8347
Size: 8507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 2206 2918 4733 6332 6764 7511 7779 767
Size: 1439 712 1815 1599 432 747 268 1495
Fragments arranged by size:
1815 1599 1495 1439 747 712 432 268
AflIII A'CryG_T
Cuts at: 866 1054 1434 5859 6963 7940 866
Size: 188 380 4425 1104 977 1433
Fragments arranged by size:
4425 1433 1104 977 380 188
AgeI A'CCGG_T
Cuts at: 6895 6895
Size: 8507
AleI CACnn'nnGTG
Cuts at: 3455 3567 4723 3455
Size: 112 1156 7239
Fragments arranged by size:
7239 1156 112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2135 2778 3545 5114 2135
Size: 643 767 1569 5528
Fragments arranged by size:
5528 1569 767 643
AlwNI CAG_nnn'CTG
Cuts at: 147 822 1543 1929 2047 2514 2633 3800 4770
Size: 675 721 386 118 467 119 1167 970
Cuts at: 4770 4866 4996 5022 5812 7379 147
Size: 96 130 26 790 1567 1275
Fragments arranged by size:
1567 1275 1167 970 790 721 675 467
386 130 119 118 96 26
ApaI G_GGCC'C
Cuts at: 3328 4476 4581 3328
Size: 1148 105 7254
Fragments arranged by size:
7254 1148 105
ApaLI G'TGCA_C
Cuts at: 983 1024 7277 983
Size: 41 6253 2213
Fragments arranged by size:
6253 2213 41
ApoI r'AATT_y
Cuts at: 5511 7860 8116 5511
Size: 2349 256 5902
Fragments arranged by size:
5902 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8507
AvaI C'yCGr_G
Cuts at: 1829 1870 3943 5321 5527 1829
Size: 41 2073 1378 206 4809
Fragments arranged by size:
4809 2073 1378 206 41
BamHI G'GATC_C
Cuts at: 1314 1573 1698 3093 5532 1314
Size: 259 125 1395 2439 4289
Fragments arranged by size:
4289 2439 1395 259 125
BanI G'GyrC_C
Cuts at: 389 999 1256 2079 2370 2443 2537 2671 3053
Size: 610 257 823 291 73 94 134 382
Cuts at: 3053 3781 3844 4090 4341 4381 4975 5377 5523
Size: 728 63 246 251 40 594 402 146
Cuts at: 5523 5937 6185 6220 389
Size: 414 248 35 2676
Fragments arranged by size:
2676 823 728 610 594 414 402 382
291 257 251 248 246 146 134 94
73 63 40 35
BanII G_rGCy'C
Cuts at: 1271 1484 1994 3328 3500 4476 4581 4736 5071
Size: 213 510 1334 172 976 105 155 335
Cuts at: 5071 5088 5521 6551 1271
Size: 17 433 1030 3227
Fragments arranged by size:
3227 1334 1030 976 510 433 335 213
172 155 105 17
BbeI G_GCGC'C
Cuts at: 393 1260 2541 5941 6189 393
Size: 867 1281 3400 248 2711
Fragments arranged by size:
3400 2711 1281 867 248
BbvCI CC'TCA_GC
Cuts at: 1013 1115 1820 3858 4155 4426 4528 4898 1013
Size: 102 705 2038 297 271 102 370 4622
Fragments arranged by size:
4622 2038 705 370 297 271 102 102
BciVI GTATCCnnnnn_n'
Cuts at: 505 6405 7166 8507 505
Size: 5900 761 1341 505
Fragments arranged by size:
5900 1341 761 505
BclI T'GATC_A
Cuts at: 2799 5229 6027 2799
Size: 2430 798 5279
Fragments arranged by size:
5279 2430 798
BfrBI ATG'CAT
Cuts at: 3682 3682
Size: 8507
BglI GCCn_nnn'nGGC
Cuts at: 199 320 997 1469 1628 1637 2585 2705 2981
Size: 121 677 472 159 9 948 120 276
Cuts at: 2981 3461 4954 5197 199
Size: 480 1493 243 3509
Fragments arranged by size:
3509 1493 948 677 480 472 276 243
159 121 120 9
BglII A'GATC_T
Cuts at: 5483 6022 8059 5483
Size: 539 2037 5931
Fragments arranged by size:
5931 2037 539
BlpI GC'TnA_GC
Cuts at: 178 600 1483 4754 5201 5258 5619 178
Size: 422 883 3271 447 57 361 3066
Fragments arranged by size:
3271 3066 883 447 422 361 57
Bme1580I G_kGCm'C
Cuts at: 987 1002 1028 1163 1447 1537 1720 2084 2214
Size: 15 26 135 284 90 183 364 130
Cuts at: 2214 2375 2448 2559 2622 3015 3023 3168 3241
Size: 161 73 111 63 393 8 145 73
Cuts at: 3241 3259 3304 3328 3348 3639 3849 4275 4344
Size: 18 45 24 20 291 210 426 69
Cuts at: 4344 4476 4506 4581 4959 5064 5181 5193 5403
Size: 132 30 75 378 105 117 12 210
Cuts at: 5403 6132 6225 7281 987
Size: 729 93 1056 2213
Fragments arranged by size:
2213 1056 729 426 393 378 364 291
284 210 210 183 161 145 135 132
130 117 111 105 93 90 75 73
73 69 63 45 30 26 24 20
18 15 12 8
BmgBI CAC'GTC
Cuts at: 1606 1683 1606
Size: 77 8430
BmrI ACTGGGnnnn_n'
Cuts at: 175 705 1408 1644 1698 3513 4474 5415 5719
Size: 530 703 236 54 1815 961 941 304
Cuts at: 5719 5800 6135 8279 175
Size: 81 335 2144 403
Fragments arranged by size:
2144 1815 961 941 703 530 403 335
304 236 81 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 6874 135
Size: 6739 1768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1951 2341 2546 4027 4960 5128 5425 544
Size: 1407 390 205 1481 933 168 297 3626
Fragments arranged by size:
3626 1481 1407 933 390 297 205 168
Bpu10I CC'TnA_GC
Cuts at: 1013 1115 1642 1820 1896 2003 3858 4155 4306
Size: 102 527 178 76 107 1855 297 151
Cuts at: 4306 4426 4528 4898 5342 1013
Size: 120 102 370 444 4178
Fragments arranged by size:
4178 1855 527 444 370 297 178 151
120 107 102 102 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 1343 2176 2453 5683 6562 7054 7352 105
Size: 1238 833 277 3230 879 492 298 1260
Fragments arranged by size:
3230 1260 1238 879 833 492 298 277
BsaI GGTCTCn'nnnn_
Cuts at: 1413 3503 8211 1413
Size: 2090 4708 1709
Fragments arranged by size:
4708 2090 1709
BsaAI yAC'GTr
Cuts at: 946 1057 4831 5860 946
Size: 111 3774 1029 3593
Fragments arranged by size:
3774 3593 1029 111
BsaBI GATnn'nnATC
Cuts at: 6 1088 6021 8058 6
Size: 1082 4933 2037 455
Fragments arranged by size:
4933 2037 1082 455
BsaHI Gr'CG_yC
Cuts at: 390 1107 1257 2538 5938 6186 6888 8384 390
Size: 717 150 1281 3400 248 702 1496 513
Fragments arranged by size:
3400 1496 1281 717 702 513 248 150
BsaWI w'CCGG_w
Cuts at: 4796 5696 6217 6895 7169 7316 7727 4796
Size: 900 521 678 274 147 411 5576
Fragments arranged by size:
5576 900 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 517
Size: 30 8477
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2374 2401 3910 4957 2374
Size: 27 1509 1047 5924
Fragments arranged by size:
5924 1509 1047 27
BseYI C'CCAG_C
Cuts at: 326 1252 2213 2374 2954 3234 3777 3819 4743
Size: 926 961 161 580 280 543 42 924
Cuts at: 4743 4892 4949 5932 6690 7267 7763 326
Size: 149 57 983 758 577 496 1070
Fragments arranged by size:
1070 983 961 926 924 758 580 577
543 496 280 161 149 57 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 2053 2625 3090 3204 3730 3850 3857 5072
Size: 674 572 465 114 526 120 7 1215
Cuts at: 5072 5270 5500 1379
Size: 198 230 4386
Fragments arranged by size:
4386 1215 674 572 526 465 230 198
120 114 7
BsiEI CG_ry'CG
Cuts at: 104 6049 6095 7303 104
Size: 5945 46 1208 1308
Fragments arranged by size:
5945 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 987 1028 1271 1552 1844 2819 3500 3932 4200
Size: 41 243 281 292 975 681 432 268
Cuts at: 4200 4736 5071 5521 6299 6489 7281 987
Size: 536 335 450 778 190 792 2213
Fragments arranged by size:
2213 975 792 778 681 536 450 432
335 292 281 268 243 190 41
Bsp1286I G_dGCh'C
Cuts at: 987 1002 1028 1163 1271 1447 1484 1537 1552
Size: 15 26 135 108 176 37 53 15
Cuts at: 1552 1720 1844 1994 2084 2214 2375 2448 2559
Size: 168 124 150 90 130 161 73 111
Cuts at: 2559 2622 2819 3015 3023 3168 3241 3259 3304
Size: 63 197 196 8 145 73 18 45
Cuts at: 3304 3328 3348 3500 3639 3849 3932 4200 4275
Size: 24 20 152 139 210 83 268 75
Cuts at: 4275 4344 4476 4506 4581 4736 4959 5064 5071
Size: 69 132 30 75 155 223 105 7
Cuts at: 5071 5088 5181 5193 5403 5521 6132 6225 6299
Size: 17 93 12 210 118 611 93 74
Cuts at: 6299 6489 6551 7281 987
Size: 190 62 730 2213
Fragments arranged by size:
2213 730 611 268 223 210 210 197
196 190 176 168 161 155 152 150
145 139 135 132 130 124 118 111
108 105 93 93 90 83 75 75
74 73 73 69 63 62 53 45
37 30 26 24 20 18 17 15
15 12 8 7
BspEI T'CCGG_A
Cuts at: 4796 7727 4796
Size: 2931 5576
BspHI T'CATG_A
Cuts at: 7683 7683
Size: 8507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 359 1106 1316 1661 1758 1827 1982 3239
Size: 84 747 210 345 97 69 155 1257
Cuts at: 3239 3433 5038 5065 5466 5503 5557 5771 6073
Size: 194 1605 27 401 37 54 214 302
Cuts at: 6073 6454 275
Size: 381 2328
Fragments arranged by size:
2328 1605 1257 747 401 381 345 302
214 210 194 155 97 84 69 54
37 27
BsrBI CCG'CTC
Cuts at: 50 1331 5058 5341 5397 6799 6853 8337 8502
Size: 1281 3727 283 56 1402 54 1484 165
Cuts at: 8502 50
Size: 55
Fragments arranged by size:
3727 1484 1402 1281 283 165 56 55
54
BsrDI GCAATG_nn'
Cuts at: 1215 1465 2014 2659 2751 2831 3130 4440 6419
Size: 250 549 645 92 80 299 1310 1979
Cuts at: 6419 1215
Size: 3303
Fragments arranged by size:
3303 1979 1310 645 549 299 250 92
80
BsrFI r'CCGG_y
Cuts at: 1508 2729 2879 3050 3460 4384 6505 6686 6895
Size: 1221 150 171 410 924 2121 181 209
Cuts at: 6895 1508
Size: 3120
Fragments arranged by size:
3120 2121 1221 924 410 209 181 171
150
BssSI C'ACGA_G
Cuts at: 2611 3548 6778 7136 2611
Size: 937 3230 358 3982
Fragments arranged by size:
3982 3230 937 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1451 2079 3129 3759 4360 4866 5176 5884 1451
Size: 628 1050 630 601 506 310 708 4074
Fragments arranged by size:
4074 1050 708 630 628 601 506 310
BstBI TT'CG_AA
Cuts at: 6868 6868
Size: 8507
BstEII G'GTnAC_C
Cuts at: 504 1863 504
Size: 1359 7148
BstXI CCAn_nnnn'nTGG
Cuts at: 516 591 695 516
Size: 75 104 8328
Fragments arranged by size:
8328 104 75
BstYI r'GATC_y
Cuts at: 1 902 1314 1573 1698 3093 4760 5483 5532
Size: 901 412 259 125 1395 1667 723 49
Cuts at: 5532 5999 6022 6357 6603 7615 7701 7713 8059
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8059 1
Size: 449
Fragments arranged by size:
1667 1395 1012 901 723 467 449 412
346 335 259 246 125 86 49 23
12
BstZ17I GTA'TAC
Cuts at: 1194 1194
Size: 8507
BtgI C'CryG_G
Cuts at: 107 257 688 892 1155 1349 2844 2854 3186
Size: 150 431 204 263 194 1495 10 332
Cuts at: 3186 3375 3639 107
Size: 189 264 4975
Fragments arranged by size:
4975 1495 431 332 264 263 204 194
189 150 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 409 1527 3012 6457 6638 6801 409
Size: 1118 1485 3445 181 163 2115
Fragments arranged by size:
3445 2115 1485 1118 181 163
BtsI GCAGTG_nn'
Cuts at: 1081 1731 2970 3078 3168 3259 3297 3304 3450
Size: 650 1239 108 90 91 38 7 146
Cuts at: 3450 3451 3529 3588 3869 4036 4997 5181 5750
Size: 1 78 59 281 167 961 184 569
Cuts at: 5750 1081
Size: 3838
Fragments arranged by size:
3838 1239 961 650 569 281 184 167
146 108 91 90 78 59 38 7
1
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1038 1073 1197 1232 1792 1827 1038
Size: 35 124 35 560 35 7718
Fragments arranged by size:
7718 560 124 35 35 35
DraI TTT'AAA
Cuts at: 5506 5506
Size: 8507
DraIII CAC_nnn'GTG
Cuts at: 3163 4000 4360 3163
Size: 837 360 7310
Fragments arranged by size:
7310 837 360
DrdI GACnn_nn'nnGTC
Cuts at: 3209 6213 7071 3209
Size: 3004 858 4645
Fragments arranged by size:
4645 3004 858
EaeI y'GGCC_r
Cuts at: 612 1122 1187 1413 1629 2706 2731 3048 3372
Size: 510 65 226 216 1077 25 317 324
Cuts at: 3372 4259 4715 6092 6266 6657 6684 8029 612
Size: 887 456 1377 174 391 27 1345 1090
Fragments arranged by size:
1377 1345 1090 1077 887 510 456 391
324 317 226 216 174 65 27 25
EagI C'GGCC_G
Cuts at: 6092 6092
Size: 8507
EarI CTCTTCn'nnn_
Cuts at: 6530 6740 6530
Size: 210 8297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3489 6715 7025 7171 8317 3489
Size: 3226 310 146 1146 3679
Fragments arranged by size:
3679 3226 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1269 3498 4734 5069 5519 1269
Size: 2229 1236 335 450 4257
Fragments arranged by size:
4257 2229 1236 450 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 767 1951 2206 2341 2546 2918 4027 4733
Size: 223 1184 255 135 205 372 1109 706
Cuts at: 4733 4960 5128 5425 6332 6764 7511 7779 544
Size: 227 168 297 907 432 747 268 1272
Fragments arranged by size:
1272 1184 1109 907 747 706 432 372
297 268 255 227 223 205 168 135
EcoNI CCTnn'n_nnAGG
Cuts at: 4594 4678 5026 4594
Size: 84 348 8075
Fragments arranged by size:
8075 348 84
EcoO109I rG'GnC_Cy
Cuts at: 207 1305 1638 1998 2044 2850 2902 3030 3324
Size: 1098 333 360 46 806 52 128 294
Cuts at: 3324 3325 3418 4422 5250 5325 5646 207
Size: 1 93 1004 828 75 321 3068
Fragments arranged by size:
3068 1098 1004 828 806 360 333 321
294 128 93 75 52 46 1
EcoRI G'AATT_C
Cuts at: 5511 5511
Size: 8507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 387 3198 4257 6526 387
Size: 2811 1059 2269 2368
Fragments arranged by size:
2811 2368 2269 1059
FspI TGC'GCA
Cuts at: 2403 5449 6288 2403
Size: 3046 839 4622
Fragments arranged by size:
4622 3046 839
HaeII r_GCGC'y
Cuts at: 393 1260 1860 2328 2541 2783 3738 5941 6189
Size: 867 600 468 213 242 955 2203 248
Cuts at: 6189 7211 8190 393
Size: 1022 979 710
Fragments arranged by size:
2203 1022 979 955 867 710 600 468
248 242 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1022 1054 3529 3561 4212 4244 6349 6379 6381
Size: 32 2475 32 651 32 2105 30 2
Cuts at: 6381 6411 1022
Size: 30 3118
Fragments arranged by size:
3118 2475 2105 651 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 5546 5546
Size: 8507
HindIII A'AGCT_T
Cuts at: 3005 3005
Size: 8507
KasI G'GCGC_C
Cuts at: 389 1256 2537 5937 6185 389
Size: 867 1281 3400 248 2711
Fragments arranged by size:
3400 2711 1281 867 248
KpnI G_GTAC'C
Cuts at: 5527 5527
Size: 8507
MluI A'CGCG_T
Cuts at: 7940 7940
Size: 8507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2000 5075 5405 5414 7178 7362 2000
Size: 3075 330 9 1764 184 3145
Fragments arranged by size:
3145 3075 1764 330 184 9
MscI TGG'CCA
Cuts at: 1631 2708 3374 4261 4717 6268 1631
Size: 1077 666 887 456 1551 3870
Fragments arranged by size:
3870 1551 1077 887 666 456
MslI CAynn'nnrTG
Cuts at: 589 698 1130 2457 2756 2942 3455 3533 3567
Size: 109 432 1327 299 186 513 78 34
Cuts at: 3567 4708 4723 6496 6623 589
Size: 1141 15 1773 127 2473
Fragments arranged by size:
2473 1773 1327 1141 513 432 299 186
127 109 78 34 15
MspA1I CmG'CkG
Cuts at: 462 516 1172 1252 1619 2543 2696 3218 3341
Size: 54 656 80 367 924 153 522 123
Cuts at: 3341 3596 3747 4269 4430 4444 4500 4575 4753
Size: 255 151 522 161 14 56 75 178
Cuts at: 4753 4953 5208 5358 5618 5932 6292 7305 7550
Size: 200 255 150 260 314 360 1013 245
Cuts at: 7550 462
Size: 1419
Fragments arranged by size:
1419 1013 924 656 522 522 367 360
314 260 255 255 245 200 178 161
153 151 150 123 80 75 56 54
14
NaeI GCC'GGC
Cuts at: 1510 2731 2881 3462 4386 6688 1510
Size: 1221 150 581 924 2302 3329
Fragments arranged by size:
3329 2302 1221 924 581 150
NarI GG'CG_CC
Cuts at: 390 1257 2538 5938 6186 390
Size: 867 1281 3400 248 2711
Fragments arranged by size:
3400 2711 1281 867 248
NcoI C'CATG_G
Cuts at: 107 1155 1349 3186 3375 107
Size: 1048 194 1837 189 5239
Fragments arranged by size:
5239 1837 1048 194 189
NdeI CA'TA_TG
Cuts at: 2865 2865
Size: 8507
NgoMIV G'CCGG_C
Cuts at: 1508 2729 2879 3460 4384 6686 1508
Size: 1221 150 581 924 2302 3329
Fragments arranged by size:
3329 2302 1221 924 581 150
NsiI A_TGCA'T
Cuts at: 3684 3684
Size: 8507
NspI r_CATG'y
Cuts at: 628 1438 2122 2755 3227 3686 3810 4114 5560
Size: 810 684 633 472 459 124 304 1446
Cuts at: 5560 6060 6967 628
Size: 500 907 2168
Fragments arranged by size:
2168 1446 907 810 684 633 500 472
459 304 124
PasI CC'CwG_GG
Cuts at: 152 994 2905 3016 3514 3604 4971 5181 152
Size: 842 1911 111 498 90 1367 210 3478
Fragments arranged by size:
3478 1911 1367 842 498 210 111 90
PciI A'CATG_T
Cuts at: 1434 6963 1434
Size: 5529 2978
PflMI CCAn_nnn'nTGG
Cuts at: 1746 3292 3356 4770 1746
Size: 1546 64 1414 5483
Fragments arranged by size:
5483 1546 1414 64
PfoI T'CCnGG_A
Cuts at: 1796 1885 5105 1796
Size: 89 3220 5198
Fragments arranged by size:
5198 3220 89
PmeI GTTT'AAAC
Cuts at: 5506 5506
Size: 8507
PmlI CAC'GTG
Cuts at: 1057 1057
Size: 8507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7646 7678 7646
Size: 32 8475
PpuMI rG'GwC_Cy
Cuts at: 1305 1998 2850 3030 5325 1305
Size: 693 852 180 2295 4487
Fragments arranged by size:
4487 2295 852 693 180
PshAI GACnn'nnGTC
Cuts at: 1367 1367
Size: 8507
PspOMI G'GGCC_C
Cuts at: 3324 4472 4577 3324
Size: 1148 105 7254
Fragments arranged by size:
7254 1148 105
PspXI vC'TCGA_Gb
Cuts at: 3943 3943
Size: 8507
PstI C_TGCA'G
Cuts at: 356 3595 4131 4499 4871 5076 5357 5554 6239
Size: 3239 536 368 372 205 281 197 685
Cuts at: 6239 356
Size: 2624
Fragments arranged by size:
3239 2624 685 536 372 368 281 205
197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8507
PvuII CAG'CTG
Cuts at: 516 1172 1252 3596 4430 4753 5208 5932 6292
Size: 656 80 2344 834 323 455 724 360
Cuts at: 6292 516
Size: 2731
Fragments arranged by size:
2731 2344 834 724 656 455 360 323
80
RsrII CG'GwC_CG
Cuts at: 6702 6702
Size: 8507
SacI G_AGCT'C
Cuts at: 1271 3500 4736 5071 5521 1271
Size: 2229 1236 335 450 4257
Fragments arranged by size:
4257 2229 1236 450 335
SalI G'TCGA_C
Cuts at: 5544 5544
Size: 8507
SanDI GG'GwC_CC
Cuts at: 2850 3030 2850
Size: 180 8327
SapI GCTCTTCn'nnn_
Cuts at: 6530 6740 6530
Size: 210 8297
SbfI CC_TGCA'GG
Cuts at: 356 5554 356
Size: 5198 3309
ScaI AGT'ACT
Cuts at: 916 8024 916
Size: 7108 1399
SexAI A'CCwGG_T
Cuts at: 5027 5027
Size: 8507
SfcI C'TryA_G
Cuts at: 33 352 801 1137 1912 3591 4127 4448 4495
Size: 319 449 336 775 1679 536 321 47
Cuts at: 4495 4835 4867 5072 5153 5353 5362 5550 6235
Size: 340 32 205 81 200 9 188 685
Cuts at: 6235 7228 7419 33
Size: 993 191 1121
Fragments arranged by size:
1679 1121 993 775 685 536 449 340
336 321 319 205 200 191 188 81
47 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 320 1637 320
Size: 1317 7190
SfoI GGC'GCC
Cuts at: 391 1258 2539 5939 6187 391
Size: 867 1281 3400 248 2711
Fragments arranged by size:
3400 2711 1281 867 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8507
SmaI CCC'GGG
Cuts at: 1872 5529 1872
Size: 3657 4850
SmlI C'TyrA_G
Cuts at: 120 1358 2155 2468 3943 5662 6577 7069 7331
Size: 1238 797 313 1475 1719 915 492 262
Cuts at: 7331 120
Size: 1296
Fragments arranged by size:
1719 1475 1296 1238 915 797 492 313
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8507
SphI G_CATG'C
Cuts at: 2755 3686 4114 5560 6060 2755
Size: 931 428 1446 500 5202
Fragments arranged by size:
5202 1446 931 500 428
SspI AAT'ATT
Cuts at: 2950 2950
Size: 8507
StuI AGG'CCT
Cuts at: 2398 2398
Size: 8507
StyI C'CwwG_G
Cuts at: 107 1155 1301 1349 2084 2100 3186 3375 5271
Size: 1048 146 48 735 16 1086 189 1896
Cuts at: 5271 5641 5995 107
Size: 370 354 2619
Fragments arranged by size:
2619 1896 1086 1048 735 370 354 189
146 48 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1180 1675 2785 3416 3716 3769 4078 5215 6090
Size: 495 1110 631 300 53 309 1137 875
Cuts at: 6090 6223 6682 6891 1180
Size: 133 459 209 2796
Fragments arranged by size:
2796 1137 1110 875 631 495 459 309
300 209 133 53
TatI w'GTAC_w
Cuts at: 914 4926 8022 914
Size: 4012 3096 1399
Fragments arranged by size:
4012 3096 1399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1690 1722 3138 3170 5034 5066 8024 8056 1690
Size: 32 1416 32 1864 32 2958 32 2141
Fragments arranged by size:
2958 2141 1864 1416 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6304 6304
Size: 8507
XbaI T'CTAG_A
Cuts at: 5538 5538
Size: 8507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1747 2065 3175 3637 3997 4273 4617 6002 1747
Size: 318 1110 462 360 276 344 1385 4252
Fragments arranged by size:
4252 1385 1110 462 360 344 318 276
XhoI C'TCGA_G
Cuts at: 3943 3943
Size: 8507
XmaI C'CCGG_G
Cuts at: 1870 5527 1870
Size: 3657 4850
XmnI GAAnn'nnTTC
Cuts at: 370 4669 370
Size: 4299 4208
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BcgI BmtI BsiWI BsmI BsmBI BsrGI BssHII
Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PsiI PsrI SacII SgrAI SnaBI
SrfI SwaI ZraI