Restriction map for
pF1KE6039
(Circular) (Six-base) MAPSORT of: pF1KE6039.seq Check: 9515 from: 1 to: 4064
pF1KE6039 4064 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1889 2321 3068 3336 1889
Size: 432 747 268 2617
Fragments arranged by size:
2617 747 432 268
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 360 1369 2936 360
Size: 1009 1567 1488
Fragments arranged by size:
1567 1488 1009
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 439 1089 439
Size: 650 3414
BanI G'GyrC_C
Cuts at: 688 1080 1494 1742 1777 688
Size: 392 414 248 35 2975
Fragments arranged by size:
2975 414 392 248 35
BanII G_rGCy'C
Cuts at: 1078 2108 1078
Size: 1030 3034
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BciVI GTATCCnnnnn_n'
Cuts at: 736 1962 2723 4064 736
Size: 1226 761 1341 736
Fragments arranged by size:
1341 1226 761 736
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BfrBI ATG'CAT
Cuts at: 525 525
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 707 1176 707
Size: 469 3595
Bme1580I G_kGCm'C
Cuts at: 691 1689 1782 2838 691
Size: 998 93 1056 1917
Fragments arranged by size:
1917 1056 998 93
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 1240 2119 2611 2909 1011
Size: 229 879 492 298 2166
Fragments arranged by size:
2166 879 492 298 229
BsaI GGTCTCn'nnnn_
Cuts at: 768 3768 768
Size: 3000 1064
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 756 756
Size: 4064
BseYI C'CCAG_C
Cuts at: 353 1489 2247 2824 3320 353
Size: 1136 758 577 496 1097
Fragments arranged by size:
1136 1097 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 206 668 1078 1856 2046 2838 206
Size: 462 410 778 190 792 1432
Fragments arranged by size:
1432 792 778 462 410 190
BsmI GAATG_Cn'
Cuts at: 826 826
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 206 668 691 1078 1689 1782 1856 2046 2108
Size: 462 23 387 611 93 74 190 62
Cuts at: 2108 2838 206
Size: 730 1432
Fragments arranged by size:
1432 730 611 462 387 190 93 74
62 23
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 844 1114 1328 1630 2011 136
Size: 708 270 214 302 381 2189
Fragments arranged by size:
2189 708 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 776 1976 776
Size: 1200 2864
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstEII G'GTnAC_C
Cuts at: 858 957 858
Size: 99 3965
BstXI CCAn_nnnn'nTGG
Cuts at: 114 255 585 114
Size: 141 330 3593
Fragments arranged by size:
3593 330 141
BstYI r'GATC_y
Cuts at: 1 439 913 1089 1556 1579 1914 2160 3172
Size: 438 474 176 467 23 335 246 1012
Cuts at: 3172 3258 3270 3616 1
Size: 86 12 346 449
Fragments arranged by size:
1012 474 467 449 438 346 335 246
176 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 613 2014 2195 2358 613
Size: 1401 181 163 2319
Fragments arranged by size:
2319 1401 181 163
BtsI GCAGTG_nn'
Cuts at: 348 794 1307 348
Size: 446 513 3105
Fragments arranged by size:
3105 513 446
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DraIII CAC_nnn'GTG
Cuts at: 449 789 449
Size: 340 3724
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 1649 1823 2214 2241 3586 1649
Size: 174 391 27 1345 2127
Fragments arranged by size:
2127 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 949 2087 2297 949
Size: 1138 210 2716
Fragments arranged by size:
2716 1138 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1889 2321 3068 3336 1889
Size: 432 747 268 2617
Fragments arranged by size:
2617 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 657 833 1203 657
Size: 176 370 3518
Fragments arranged by size:
3518 370 176
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1906 1936 1938 1968 1906
Size: 30 2 30 4002
Fragments arranged by size:
4002 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
HindIII A'AGCT_T
Cuts at: 277 277
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 1825 1825
Size: 4064
MslI CAynn'nnrTG
Cuts at: 112 128 886 2053 2180 112
Size: 16 758 1167 127 1996
Fragments arranged by size:
1996 1167 758 127 16
MspA1I CmG'CkG
Cuts at: 329 612 1175 1489 1849 2862 3107 329
Size: 283 563 314 360 1013 245 1286
Fragments arranged by size:
1286 1013 563 360 314 283 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 107 107
Size: 4064
NdeI CA'TA_TG
Cuts at: 284 284
Size: 4064
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NsiI A_TGCA'T
Cuts at: 527 527
Size: 4064
NspI r_CATG'y
Cuts at: 969 1117 1617 2524 969
Size: 148 500 907 2509
Fragments arranged by size:
2509 907 500 148
PasI CC'CwG_GG
Cuts at: 435 435
Size: 4064
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 360 435 360
Size: 75 3989
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PpuMI rG'GwC_Cy
Cuts at: 657 833 657
Size: 176 3888
PshAI GACnn'nnGTC
Cuts at: 856 856
Size: 4064
PstI C_TGCA'G
Cuts at: 611 899 1111 1796 611
Size: 288 212 685 2879
Fragments arranged by size:
2879 685 288 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 612 1489 1849 612
Size: 877 360 2827
Fragments arranged by size:
2827 877 360
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 607 895 1107 1792 2785 2976 33
Size: 574 288 212 685 993 191 1121
Fragments arranged by size:
1121 993 685 574 288 212 191
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 990 1219 2134 2626 2888 990
Size: 229 915 492 262 2166
Fragments arranged by size:
2166 915 492 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 969 1117 1617 969
Size: 148 500 3416
Fragments arranged by size:
3416 500 148
SspI AAT'ATT
Cuts at: 1053 1053
Size: 4064
StyI C'CwwG_G
Cuts at: 107 545 1198 1552 107
Size: 438 653 354 2619
Fragments arranged by size:
2619 653 438 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 1559 252
Size: 1307 2757
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 826 826
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI PacI
PfoI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI