Restriction map for
pF1KE6234
(Circular) (Six-base) MAPSORT of: pF1KE6234.seq Check: 6294 from: 1 to: 3998
pF1KE6234 3998 bp
With 182 enzymes: SgfI *
July 20, 2018 17:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 493 493
Size: 3998
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 311 1014 311
Size: 703 3295
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 2255 3002 3270 1823
Size: 432 747 268 2551
Fragments arranged by size:
2551 747 432 268
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 560 592 560
Size: 32 3966
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 1018 1018
Size: 3998
BamHI G'GATC_C
Cuts at: 446 1023 446
Size: 577 3421
BanI G'GyrC_C
Cuts at: 311 378 717 1014 1428 1676 1711 311
Size: 67 339 297 414 248 35 2598
Fragments arranged by size:
2598 414 339 297 248 67 35
BanII G_rGCy'C
Cuts at: 237 565 1012 2042 237
Size: 328 447 1030 2193
Fragments arranged by size:
2193 1030 447 328
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 173 621 813 982 173
Size: 448 192 169 3189
Fragments arranged by size:
3189 448 192 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 101 135 101
Size: 34 3964
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 779 1518 779
Size: 739 3259
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 722 1623 1716 2772 722
Size: 901 93 1056 1948
Fragments arranged by size:
1948 1056 901 93
BmrI ACTGGGnnnn_n'
Cuts at: 564 1210 1291 1626 3770 564
Size: 646 81 335 2144 792
Fragments arranged by size:
2144 792 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 146 2365 146
Size: 2219 1779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 976 587
Size: 389 3609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2053 2545 2843 1174
Size: 879 492 298 2329
Fragments arranged by size:
2329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 117 365 3702 117
Size: 248 3337 413
Fragments arranged by size:
3337 413 248
BsaAI yAC'GTr
Cuts at: 838 1351 838
Size: 513 3485
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 449 689 1187 1708 2386 2660 2807 3218 449
Size: 240 498 521 678 274 147 411 1229
Fragments arranged by size:
1229 678 521 498 411 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 560 590 292
Size: 30 238 30 3700
Fragments arranged by size:
3700 238 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 151 154 157 160 529 571 592 964
Size: 3 3 3 3 369 42 21 372
Cuts at: 964 971 148
Size: 7 3175
Fragments arranged by size:
3175 372 369 42 21 7 3 3
3 3
BseYI C'CCAG_C
Cuts at: 136 1423 2181 2758 3254 136
Size: 1287 758 577 496 880
Fragments arranged by size:
1287 880 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 602 1012 1790 1980 2772 602
Size: 410 778 190 792 1828
Fragments arranged by size:
1828 792 778 410 190
Bsp1286I G_dGCh'C
Cuts at: 237 565 602 722 1012 1623 1716 1790 1980
Size: 328 37 120 290 611 93 74 190
Cuts at: 1980 2042 2772 237
Size: 62 730 1463
Fragments arranged by size:
1463 730 611 328 290 190 120 93
74 62 37
BspEI T'CCGG_A
Cuts at: 449 3218 449
Size: 2769 1229
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 493 1048 1262 1564 1945 493
Size: 555 214 302 381 2546
Fragments arranged by size:
2546 555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 1910 530
Size: 1380 2618
BsrFI r'CCGG_y
Cuts at: 753 1996 2177 2386 753
Size: 1243 181 209 2365
Fragments arranged by size:
2365 1243 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 446 543 1023 1490 1513 1848 2094 3106
Size: 445 97 480 467 23 335 246 1012
Cuts at: 3106 3192 3204 3550 1
Size: 86 12 346 449
Fragments arranged by size:
1012 480 467 449 445 346 335 246
97 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 1241 1241
Size: 3998
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 1583 1757 2148 2175 3520 1583
Size: 174 391 27 1345 2061
Fragments arranged by size:
2061 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 824 852 903 2021 2231 824
Size: 28 51 1118 210 2591
Fragments arranged by size:
2591 1118 210 51 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 976 1823 2255 3002 3270 587
Size: 389 847 432 747 268 1315
Fragments arranged by size:
1315 847 747 432 389 268
EcoNI CCTnn'n_nnAGG
Cuts at: 327 327
Size: 3998
EcoO109I rG'GnC_Cy
Cuts at: 1137 1137
Size: 3998
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 1432 1680 2702 3681 1432
Size: 248 1022 979 1749
Fragments arranged by size:
1749 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 666 684 698 716 1840 1870 1872
Size: 32 422 18 14 18 1124 30 2
Cuts at: 1872 1902 212
Size: 30 2308
Fragments arranged by size:
2308 1124 422 32 30 30 18 18
14 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
HindIII A'AGCT_T
Cuts at: 114 114
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 315 1018 315
Size: 703 3295
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 1759 1759
Size: 3998
MslI CAynn'nnrTG
Cuts at: 301 1987 2114 301
Size: 1686 127 2185
Fragments arranged by size:
2185 1686 127
MspA1I CmG'CkG
Cuts at: 140 1109 1423 1783 2796 3041 140
Size: 969 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 969 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 107 107
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 1051 1551 2458 1051
Size: 500 907 2591
Fragments arranged by size:
2591 907 500
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 560 592 3137 3169 560
Size: 32 2545 32 1389
Fragments arranged by size:
2545 1389 32 32
PsiI TTA'TAA
Cuts at: 860 860
Size: 3998
PstI C_TGCA'G
Cuts at: 611 644 1045 1730 611
Size: 33 401 685 2879
Fragments arranged by size:
2879 685 401 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 140 1423 1783 140
Size: 1283 360 2355
Fragments arranged by size:
2355 1283 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 824 2021 2231 824
Size: 1197 210 2591
Fragments arranged by size:
2591 1197 210
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 290 345 607 640 1041 1726 2719 2910
Size: 257 55 262 33 401 685 993 191
Cuts at: 2910 33
Size: 1121
Fragments arranged by size:
1121 993 685 401 262 257 191 55
33
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 1153 2068 2560 2822 1153
Size: 915 492 262 2329
Fragments arranged by size:
2329 915 492 262
SnaBI TAC'GTA
Cuts at: 838 838
Size: 3998
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StyI C'CwwG_G
Cuts at: 107 767 1132 1486 107
Size: 660 365 354 2619
Fragments arranged by size:
2619 660 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 546 955 1581 1714 2173 2382 546
Size: 409 626 133 459 209 2162
Fragments arranged by size:
2162 626 459 409 209 133
TatI w'GTAC_w
Cuts at: 644 3513 644
Size: 2869 1129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 292 324 3515 3547 292
Size: 32 3191 32 743
Fragments arranged by size:
3191 743 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI ZraI