Restriction map for
pF1KE6355
(Circular) (Six-base) MAPSORT of: pF1KE6355.seq Check: 7504 from: 1 to: 4160
pF1KE6355 4160 bp
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 487 487
Size: 4160
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 414 577 956 1106 1985 2417 3164 3432
Size: 32 163 379 150 879 432 747 268
Cuts at: 3432 382
Size: 1110
Fragments arranged by size:
1110 879 747 432 379 268 163 150
32
AflIII A'CryG_T
Cuts at: 122 1512 2616 3593 122
Size: 1390 1104 977 689
Fragments arranged by size:
1390 1104 977 689
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AhdI GACnn_n'nnGTC
Cuts at: 865 865
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 1465 3032 1465
Size: 1567 2593
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 1033 1164 3513 3769 1033
Size: 131 2349 256 1424
Fragments arranged by size:
2349 1424 256 131
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 1180 1180
Size: 4160
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 567 1117 1176 1590 1838 1873 567
Size: 550 59 414 248 35 2854
Fragments arranged by size:
2854 550 414 248 59 35
BanII G_rGCy'C
Cuts at: 1107 1174 2204 1107
Size: 67 1030 3063
Fragments arranged by size:
3063 1030 67
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 345 420 345
Size: 75 4085
BbvCI CC'TCA_GC
Cuts at: 460 460
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 683 1680 683
Size: 997 3163
BglI GCCn_nnn'nGGC
Cuts at: 112 285 304 112
Size: 173 19 3968
Fragments arranged by size:
3968 173 19
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 498 572 1785 1878 2934 498
Size: 74 1213 93 1056 1724
Fragments arranged by size:
1724 1213 1056 93 74
BmrI ACTGGGnnnn_n'
Cuts at: 1372 1453 1788 3932 1372
Size: 81 335 2144 1600
Fragments arranged by size:
2144 1600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1155 2527 1155
Size: 1372 2788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 516 428
Size: 88 4072
Bpu10I CC'TnA_GC
Cuts at: 460 460
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2707 3005 1336
Size: 879 492 298 2491
Fragments arranged by size:
2491 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 685 1674 3711 6
Size: 679 989 2037 455
Fragments arranged by size:
2037 989 679 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2822 2969 3380 1349
Size: 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 514 544 514
Size: 30 4130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 774 958 994 505
Size: 269 184 36 3671
Fragments arranged by size:
3671 269 184 36
BseYI C'CCAG_C
Cuts at: 231 342 813 1585 2343 2920 3416 231
Size: 111 471 772 758 577 496 975
Fragments arranged by size:
975 772 758 577 496 471 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 720 876 222
Size: 498 156 3506
Fragments arranged by size:
3506 498 156
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1107 1174 1952 2142 2934 1107
Size: 67 778 190 792 2333
Fragments arranged by size:
2333 792 778 190 67
Bsp1286I G_dGCh'C
Cuts at: 498 572 1107 1174 1785 1878 1952 2142 2204
Size: 74 535 67 611 93 74 190 62
Cuts at: 2204 2934 498
Size: 730 1724
Fragments arranged by size:
1724 730 611 535 190 93 74 74
67 62
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 487 1210 1424 1726 2107 487
Size: 723 214 302 381 2540
Fragments arranged by size:
2540 723 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1007 2072 1007
Size: 1065 3095
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BsrGI T'GTAC_A
Cuts at: 521 521
Size: 4160
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 180 300 735 180
Size: 120 435 3605
Fragments arranged by size:
3605 435 120
BstYI r'GATC_y
Cuts at: 1 539 1185 1652 1675 2010 2256 3268 3354
Size: 538 646 467 23 335 246 1012 86
Cuts at: 3354 3366 3712 1
Size: 12 346 449
Fragments arranged by size:
1012 646 538 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 242 107
Size: 135 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 279 2110 2291 2454 279
Size: 1831 181 163 1985
Fragments arranged by size:
1985 1831 181 163
BtsI GCAGTG_nn'
Cuts at: 708 1403 708
Size: 695 3465
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 290 380 437 646 707 1745 1919 2310 2337
Size: 90 57 209 61 1038 174 391 27
Cuts at: 2337 3682 290
Size: 1345 768
Fragments arranged by size:
1345 1038 768 391 209 174 90 61
57 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 736 841 2183 2393 736
Size: 105 1342 210 2503
Fragments arranged by size:
2503 1342 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 2678 2824 3970 2368
Size: 310 146 1146 2558
Fragments arranged by size:
2558 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1105 1172 1105
Size: 67 4093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 414 428 516 577 956 1106 1985 2417
Size: 32 14 88 61 379 150 879 432
Cuts at: 2417 3164 3432 382
Size: 747 268 1110
Fragments arranged by size:
1110 879 747 432 379 268 150 88
61 32 14
EcoO109I rG'GnC_Cy
Cuts at: 552 1299 552
Size: 747 3413
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
EcoRV GAT'ATC
Cuts at: 590 590
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 285 2179 285
Size: 1894 2266
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 171 1594 1842 2864 3843 171
Size: 1423 248 1022 979 488
Fragments arranged by size:
1423 1022 979 488 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 668 700 851 883 2002 2032 2034
Size: 32 387 32 151 32 1119 30 2
Cuts at: 2034 2064 249
Size: 30 2345
Fragments arranged by size:
2345 1119 387 151 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 122 3593 122
Size: 3471 689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 921 2831 3015 921
Size: 1910 184 2066
Fragments arranged by size:
2066 1910 184
MscI TGG'CCA
Cuts at: 292 382 439 1921 292
Size: 90 57 1482 2531
Fragments arranged by size:
2531 1482 90 57
MslI CAynn'nnrTG
Cuts at: 199 469 733 2149 2276 199
Size: 270 264 1416 127 2083
Fragments arranged by size:
2083 1416 270 264 127
MspA1I CmG'CkG
Cuts at: 1075 1271 1585 1945 2958 3203 1075
Size: 196 314 360 1013 245 2032
Fragments arranged by size:
2032 1013 360 314 245 196
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 242 107
Size: 135 4025
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 660 1213 1713 2620 660
Size: 553 500 907 2200
Fragments arranged by size:
2200 907 553 500
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 1019 1019
Size: 4160
PfoI T'CCnGG_A
Cuts at: 1098 1098
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PpuMI rG'GwC_Cy
Cuts at: 552 552
Size: 4160
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 1075 1585 1945 1075
Size: 510 360 3290
Fragments arranged by size:
3290 510 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1107 1174 1107
Size: 67 4093
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 736 2183 2393 736
Size: 1447 210 2503
Fragments arranged by size:
2503 1447 210
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 1203 1888 2881 3072 33
Size: 1170 685 993 191 1121
Fragments arranged by size:
1170 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1182 1182
Size: 4160
SmlI C'TyrA_G
Cuts at: 1315 2230 2722 2984 1315
Size: 915 492 262 2491
Fragments arranged by size:
2491 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
StyI C'CwwG_G
Cuts at: 107 242 498 1046 1294 1648 107
Size: 135 256 548 248 354 2619
Fragments arranged by size:
2619 548 354 256 248 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 168 1743 1876 2335 2544 168
Size: 1575 133 459 209 1784
Fragments arranged by size:
1784 1575 459 209 133
TatI w'GTAC_w
Cuts at: 521 3675 521
Size: 3154 1006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 918 1655 300
Size: 618 737 2805
Fragments arranged by size:
2805 737 618
XmaI C'CCGG_G
Cuts at: 1180 1180
Size: 4160
XmnI GAAnn'nnTTC
Cuts at: 998 1094 998
Size: 96 4064
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI