Restriction map for
pF1KE6626
(Circular) (Six-base) MAPSORT of: pF1KE6626.seq Check: 166 from: 1 to: 4055
pF1KE6626 4055 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1880 2312 3059 3327 703
Size: 1177 432 747 268 1431
Fragments arranged by size:
1431 1177 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 861 1360 2927 861
Size: 499 1567 1989
Fragments arranged by size:
1989 1567 499
ApaI G_GGCC'C
Cuts at: 263 263
Size: 4055
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 1059 3408 3664 1059
Size: 2349 256 1450
Fragments arranged by size:
2349 1450 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 550 1075 550
Size: 525 3530
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 114 302 1071 1485 1733 1768 114
Size: 188 769 414 248 35 2401
Fragments arranged by size:
2401 769 414 248 188 35
BanII G_rGCy'C
Cuts at: 263 348 1069 2099 263
Size: 85 721 1030 2219
Fragments arranged by size:
2219 1030 721 85
BbeI G_GCGC'C
Cuts at: 118 306 1489 1737 118
Size: 188 1183 248 2436
Fragments arranged by size:
2436 1183 248 188
BbsI GAAGACnn'nnnn_
Cuts at: 809 809
Size: 4055
BbvCI CC'TCA_GC
Cuts at: 869 869
Size: 4055
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglI GCCn_nnn'nGGC
Cuts at: 112 943 112
Size: 831 3224
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 263 1680 1773 2829 263
Size: 1417 93 1056 1489
Fragments arranged by size:
1489 1417 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 308 502 622 286
Size: 22 194 120 3719
Fragments arranged by size:
3719 194 120 22
Bpu10I CC'TnA_GC
Cuts at: 869 869
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 715 3759 715
Size: 3044 1011
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 115 303 1486 1734 2436 3932 115
Size: 188 1183 248 702 1496 238
Fragments arranged by size:
1496 1183 702 248 238 188
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 289 397 424 499 535 550 598 601
Size: 51 108 27 75 36 15 48 3
Cuts at: 601 619 622 634 637 640 658 682 718
Size: 18 3 12 3 3 18 24 36
Cuts at: 718 238
Size: 3575
Fragments arranged by size:
3575 108 75 51 48 36 36 27
24 18 18 15 12 3 3 3
3
BseYI C'CCAG_C
Cuts at: 251 335 347 506 557 653 1044 1480 2238
Size: 84 12 159 51 96 391 436 758
Cuts at: 2238 2815 3311 251
Size: 577 496 995
Fragments arranged by size:
995 758 577 496 436 391 159 96
84 51 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 4055
BsiEI CG_ry'CG
Cuts at: 104 900 1597 1643 2851 104
Size: 796 697 46 1208 1308
Fragments arranged by size:
1308 1208 796 697 46
BsiHKAI G_wGCw'C
Cuts at: 797 1069 1847 2037 2829 797
Size: 272 778 190 792 2023
Fragments arranged by size:
2023 792 778 272 190
Bsp1286I G_dGCh'C
Cuts at: 263 348 797 1069 1680 1773 1847 2037 2099
Size: 85 449 272 611 93 74 190 62
Cuts at: 2099 2829 263
Size: 730 1489
Fragments arranged by size:
1489 730 611 449 272 190 93 85
74 62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 869 1105 1319 1621 2002 869
Size: 236 214 302 381 2922
Fragments arranged by size:
2922 381 302 236 214
BsrBI CCG'CTC
Cuts at: 50 540 591 2347 2401 3885 4050 50
Size: 490 51 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 490 165 55 54 51
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 199 2053 2234 2443 199
Size: 1854 181 209 1811
Fragments arranged by size:
1854 1811 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261
Size: 1079 467 23 335 246 1012 86 12
Cuts at: 3261 3607 1
Size: 346 449
Fragments arranged by size:
1079 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 272 272
Size: 4055
BtgI C'CryG_G
Cuts at: 107 941 107
Size: 834 3221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 963 2005 2186 2349 963
Size: 1042 181 163 2669
Fragments arranged by size:
2669 1042 181 163
BtsI GCAGTG_nn'
Cuts at: 211 1298 211
Size: 1087 2968
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 201 935 944 1640 1814 2205 2232 3577 201
Size: 734 9 696 174 391 27 1345 679
Fragments arranged by size:
1345 734 696 679 391 174 27 9
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 169 2078 2288 169
Size: 1909 210 1936
Fragments arranged by size:
1936 1909 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 308 502 622 703 1880 2312 3059 3327
Size: 22 194 120 81 1177 432 747 268
Cuts at: 3327 286
Size: 1014
Fragments arranged by size:
1177 1014 747 432 268 194 120 81
22
EcoNI CCTnn'n_nnAGG
Cuts at: 403 604 403
Size: 201 3854
EcoO109I rG'GnC_Cy
Cuts at: 260 312 858 1194 260
Size: 52 546 336 3121
Fragments arranged by size:
3121 546 336 52
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 708 2074 708
Size: 1366 2689
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 118 306 1489 1737 2759 3738 118
Size: 188 1183 248 1022 979 435
Fragments arranged by size:
1183 1022 979 435 248 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 724 756 1897 1927 1929 1959 724
Size: 32 1141 30 2 30 2820
Fragments arranged by size:
2820 1141 32 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 114 302 1485 1733 114
Size: 188 1183 248 2436
Fragments arranged by size:
2436 1183 248 188
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 981 2726 2910 981
Size: 1745 184 2126
Fragments arranged by size:
2126 1745 184
MscI TGG'CCA
Cuts at: 946 1816 946
Size: 870 3185
MslI CAynn'nnrTG
Cuts at: 2044 2171 2044
Size: 127 3928
MspA1I CmG'CkG
Cuts at: 351 433 657 963 1166 1480 1840 2853 3098
Size: 82 224 306 203 314 360 1013 245
Cuts at: 3098 351
Size: 1308
Fragments arranged by size:
1308 1013 360 314 306 245 224 203
82
NaeI GCC'GGC
Cuts at: 201 2236 201
Size: 2035 2020
NarI GG'CG_CC
Cuts at: 115 303 1486 1734 115
Size: 188 1183 248 2436
Fragments arranged by size:
2436 1183 248 188
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 199 2234 199
Size: 2035 2020
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PflMI CCAn_nnn'nTGG
Cuts at: 354 354
Size: 4055
PfoI T'CCnGG_A
Cuts at: 854 854
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PpuMI rG'GwC_Cy
Cuts at: 312 858 312
Size: 546 3509
PshAI GACnn'nnGTC
Cuts at: 552 552
Size: 4055
PspOMI G'GGCC_C
Cuts at: 259 259
Size: 4055
PspXI vC'TCGA_Gb
Cuts at: 550 550
Size: 4055
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 328 1098 1783 2776 2967 33
Size: 295 770 685 993 191 1121
Fragments arranged by size:
1121 993 770 685 295 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 943 943
Size: 4055
SfoI GGC'GCC
Cuts at: 116 304 1487 1735 116
Size: 188 1183 248 2436
Fragments arranged by size:
2436 1183 248 188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 550 1210 2125 2617 2879 550
Size: 660 915 492 262 1726
Fragments arranged by size:
1726 915 660 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StuI AGG'CCT
Cuts at: 297 691 297
Size: 394 3661
StyI C'CwwG_G
Cuts at: 107 785 1189 1543 107
Size: 678 404 354 2619
Fragments arranged by size:
2619 678 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1638 1771 2230 2439 1638
Size: 133 459 209 3254
Fragments arranged by size:
3254 459 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XhoI C'TCGA_G
Cuts at: 550 550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PsiI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI