Restriction map for
pF1KE6669
(Circular) (Six-base) MAPSORT of: pF1KE6669.seq Check: 6532 from: 1 to: 4001
pF1KE6669 4001 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 1039 1039
Size: 4001
Acc65I G'GTAC_C
Cuts at: 1017 1017
Size: 4001
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1826 2258 3005 3273 1826
Size: 432 747 268 2554
Fragments arranged by size:
2554 747 432 268
AflIII A'CryG_T
Cuts at: 1353 2457 3434 1353
Size: 1104 977 1920
Fragments arranged by size:
1920 1104 977
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AhdI GACnn_n'nnGTC
Cuts at: 716 716
Size: 4001
AlwNI CAG_nnn'CTG
Cuts at: 435 1306 2873 435
Size: 871 1567 1563
Fragments arranged by size:
1567 1563 871
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 502 1005 3354 3610 502
Size: 503 2349 256 893
Fragments arranged by size:
2349 893 503 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 121 250 1021 121
Size: 129 771 3101
Fragments arranged by size:
3101 771 129
BamHI G'GATC_C
Cuts at: 1026 1026
Size: 4001
BanI G'GyrC_C
Cuts at: 1017 1431 1679 1714 1017
Size: 414 248 35 3304
Fragments arranged by size:
3304 414 248 35
BanII G_rGCy'C
Cuts at: 122 251 340 1015 2045 122
Size: 129 89 675 1030 2078
Fragments arranged by size:
2078 1030 675 129 89
BbeI G_GCGC'C
Cuts at: 1435 1683 1435
Size: 248 3753
BbvCI CC'TCA_GC
Cuts at: 207 207
Size: 4001
BciVI GTATCCnnnnn_n'
Cuts at: 1899 2660 4001 1899
Size: 761 1341 1899
Fragments arranged by size:
1899 1341 761
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglI GCCn_nnn'nGGC
Cuts at: 164 164
Size: 4001
BglII A'GATC_T
Cuts at: 1516 3553 1516
Size: 2037 1964
BlpI GC'TnA_GC
Cuts at: 449 1113 449
Size: 664 3337
Bme1580I G_kGCm'C
Cuts at: 1626 1719 2775 1626
Size: 93 1056 2852
Fragments arranged by size:
2852 1056 93
BmgBI CAC'GTC
Cuts at: 387 387
Size: 4001
BmrI ACTGGGnnnn_n'
Cuts at: 1213 1294 1629 3773 1213
Size: 81 335 2144 1441
Fragments arranged by size:
2144 1441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 742 324
Size: 418 3583
Bpu10I CC'TnA_GC
Cuts at: 207 428 207
Size: 221 3780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2056 2548 2846 1177
Size: 879 492 298 2332
Fragments arranged by size:
2332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 642 3705 642
Size: 3063 938
BsaAI yAC'GTr
Cuts at: 1354 1354
Size: 4001
BsaBI GATnn'nnATC
Cuts at: 6 1515 3552 6
Size: 1509 2037 455
Fragments arranged by size:
2037 1509 455
BsaHI Gr'CG_yC
Cuts at: 1432 1680 2382 3878 1432
Size: 248 702 1496 1555
Fragments arranged by size:
1555 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1190 1711 2389 2663 2810 3221 1190
Size: 521 678 274 147 411 1970
Fragments arranged by size:
1970 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 547 490
Size: 57 3944
BseYI C'CCAG_C
Cuts at: 1426 2184 2761 3257 1426
Size: 758 577 496 2170
Fragments arranged by size:
2170 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 4001
BsiEI CG_ry'CG
Cuts at: 104 425 1543 1589 2797 104
Size: 321 1118 46 1208 1308
Fragments arranged by size:
1308 1208 1118 321 46
BsiHKAI G_wGCw'C
Cuts at: 251 340 1015 1793 1983 2775 251
Size: 89 675 778 190 792 1477
Fragments arranged by size:
1477 792 778 675 190 89
BsmI GAATG_Cn'
Cuts at: 117 604 117
Size: 487 3514
Bsp1286I G_dGCh'C
Cuts at: 122 251 340 1015 1626 1719 1793 1983 2045
Size: 129 89 675 611 93 74 190 62
Cuts at: 2045 2775 122
Size: 730 1348
Fragments arranged by size:
1348 730 675 611 190 129 93 89
74 62
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 548 1051 1265 1567 1948 548
Size: 503 214 302 381 2601
Fragments arranged by size:
2601 503 381 302 214
BsrBI CCG'CTC
Cuts at: 50 427 588 2293 2347 3831 3996 50
Size: 377 161 1705 54 1484 165 55
Fragments arranged by size:
1705 1484 377 165 161 55 54
BsrDI GCAATG_nn'
Cuts at: 1913 1913
Size: 4001
BsrFI r'CCGG_y
Cuts at: 163 1999 2180 2389 163
Size: 1836 181 209 1775
Fragments arranged by size:
1836 1775 209 181
BssSI C'ACGA_G
Cuts at: 472 2272 2630 472
Size: 1800 358 1843
Fragments arranged by size:
1843 1800 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 1378
Size: 4001
BstBI TT'CG_AA
Cuts at: 2362 2362
Size: 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 749 749
Size: 4001
BstYI r'GATC_y
Cuts at: 1 968 1026 1493 1516 1851 2097 3109 3195
Size: 967 58 467 23 335 246 1012 86
Cuts at: 3195 3207 3553 1
Size: 12 346 449
Fragments arranged by size:
1012 967 467 449 346 335 246 86
58 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 336 945 1244 336
Size: 609 299 3093
Fragments arranged by size:
3093 609 299
DraI TTT'AAA
Cuts at: 1000 1000
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 1707 2565 1707
Size: 858 3143
EaeI y'GGCC_r
Cuts at: 156 422 1586 1760 2151 2178 3523 156
Size: 266 1164 174 391 27 1345 634
Fragments arranged by size:
1345 1164 634 391 266 174 27
EagI C'GGCC_G
Cuts at: 422 1586 422
Size: 1164 2837
EarI CTCTTCn'nnn_
Cuts at: 565 578 674 959 2024 2234 565
Size: 13 96 285 1065 210 2332
Fragments arranged by size:
2332 1065 285 210 96 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2209 2519 2665 3811 2209
Size: 310 146 1146 2399
Fragments arranged by size:
2399 1146 310 146
EcoICRI GAG'CTC
Cuts at: 249 338 1013 249
Size: 89 675 3237
Fragments arranged by size:
3237 675 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 742 1826 2258 3005 3273 324
Size: 418 1084 432 747 268 1052
Fragments arranged by size:
1084 1052 747 432 418 268
EcoO109I rG'GnC_Cy
Cuts at: 766 815 1140 766
Size: 49 325 3627
Fragments arranged by size:
3627 325 49
EcoRI G'AATT_C
Cuts at: 502 1005 502
Size: 503 3498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 4001
FspI TGC'GCA
Cuts at: 1782 1782
Size: 4001
HaeII r_GCGC'y
Cuts at: 1435 1683 2705 3684 1435
Size: 248 1022 979 1752
Fragments arranged by size:
1752 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 702 734 1843 1873 1875 1905 702
Size: 32 1109 30 2 30 2798
Fragments arranged by size:
2798 1109 32 30 30 2
HincII GTy'rAC
Cuts at: 1040 1040
Size: 4001
HindIII A'AGCT_T
Cuts at: 795 795
Size: 4001
KasI G'GCGC_C
Cuts at: 1431 1679 1431
Size: 248 3753
KpnI G_GTAC'C
Cuts at: 1021 1021
Size: 4001
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 2672 2856 557
Size: 2115 184 1702
Fragments arranged by size:
2115 1702 184
MscI TGG'CCA
Cuts at: 1762 1762
Size: 4001
MslI CAynn'nnrTG
Cuts at: 747 1990 2117 747
Size: 1243 127 2631
Fragments arranged by size:
2631 1243 127
MspA1I CmG'CkG
Cuts at: 568 611 1112 1426 1786 2799 3044 568
Size: 43 501 314 360 1013 245 1525
Fragments arranged by size:
1525 1013 501 360 314 245 43
NaeI GCC'GGC
Cuts at: 165 2182 165
Size: 2017 1984
NarI GG'CG_CC
Cuts at: 1432 1680 1432
Size: 248 3753
NgoMIV G'CCGG_C
Cuts at: 163 2180 163
Size: 2017 1984
NotI GC'GGCC_GC
Cuts at: 422 422
Size: 4001
NspI r_CATG'y
Cuts at: 484 1054 1554 2461 484
Size: 570 500 907 2024
Fragments arranged by size:
2024 907 570 500
PciI A'CATG_T
Cuts at: 2457 2457
Size: 4001
PflMI CCAn_nnn'nTGG
Cuts at: 309 309
Size: 4001
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3969
PpuMI rG'GwC_Cy
Cuts at: 766 815 766
Size: 49 3952
PstI C_TGCA'G
Cuts at: 331 1048 1733 331
Size: 717 685 2599
Fragments arranged by size:
2599 717 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 568 1426 1786 568
Size: 858 360 2783
Fragments arranged by size:
2783 858 360
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 251 340 1015 251
Size: 89 675 3237
Fragments arranged by size:
3237 675 89
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 578 674 2024 2234 578
Size: 96 1350 210 2345
Fragments arranged by size:
2345 1350 210 96
SbfI CC_TGCA'GG
Cuts at: 1048 1048
Size: 4001
ScaI AGT'ACT
Cuts at: 3518 3518
Size: 4001
SexAI A'CCwGG_T
Cuts at: 768 768
Size: 4001
SfcI C'TryA_G
Cuts at: 33 327 1044 1729 2722 2913 33
Size: 294 717 685 993 191 1121
Fragments arranged by size:
1121 993 717 685 294 191
SfoI GGC'GCC
Cuts at: 1433 1681 1433
Size: 248 3753
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 1023 1023
Size: 4001
SmlI C'TyrA_G
Cuts at: 1156 2071 2563 2825 1156
Size: 915 492 262 2332
Fragments arranged by size:
2332 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
SspI AAT'ATT
Cuts at: 979 979
Size: 4001
StyI C'CwwG_G
Cuts at: 818 1135 1489 818
Size: 317 354 3330
Fragments arranged by size:
3330 354 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1584 1717 2176 2385 1584
Size: 133 459 209 3200
Fragments arranged by size:
3200 459 209 133
TatI w'GTAC_w
Cuts at: 3516 3516
Size: 4001
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 3969
Tth111I GACn'n_nGTC
Cuts at: 411 770 1798 411
Size: 359 1028 2614
Fragments arranged by size:
2614 1028 359
XbaI T'CTAG_A
Cuts at: 1032 1032
Size: 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 808 1496 306
Size: 502 688 2811
Fragments arranged by size:
2811 688 502
XmaI C'CCGG_G
Cuts at: 1021 1021
Size: 4001
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI