Restriction map for
pF1KE6679
(Circular) (Six-base) MAPSORT of: pF1KE6679.seq Check: 2006 from: 1 to: 4097
pF1KE6679 4097 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 348 1018 348
Size: 670 3427
AccI GT'mk_AC
Cuts at: 386 1135 386
Size: 749 3348
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 452 1922 2354 3101 3369 375
Size: 77 1470 432 747 268 1103
Fragments arranged by size:
1470 1103 747 432 268 77
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 1402 2969 1402
Size: 1567 2530
ApaI G_GGCC'C
Cuts at: 952 1006 1015 952
Size: 54 9 4034
Fragments arranged by size:
4034 54 9
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 125 408 618 1101 3450 3706 125
Size: 283 210 483 2349 256 516
Fragments arranged by size:
2349 516 483 283 256 210
AseI AT'TA_AT
Cuts at: 20 545 20
Size: 525 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 918 937 1008 1017 1117 918
Size: 19 71 9 100 3898
Fragments arranged by size:
3898 100 71 19 9
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 1113 1527 1775 1810 1113
Size: 414 248 35 3400
Fragments arranged by size:
3400 414 248 35
BanII G_rGCy'C
Cuts at: 952 1006 1015 1053 1111 2141 952
Size: 54 9 38 58 1030 2908
Fragments arranged by size:
2908 1030 58 54 38 9
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 4097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 596 630 596
Size: 34 4063
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 1010 1010
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 952 1006 1015 1722 1815 2871 952
Size: 54 9 707 93 1056 2178
Fragments arranged by size:
2178 1056 707 93 54 9
BmrI ACTGGGnnnn_n'
Cuts at: 413 636 1309 1390 1725 3869 413
Size: 223 673 81 335 2144 641
Fragments arranged by size:
2144 673 641 335 223 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1037 977
Size: 60 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1273 2152 2644 2942 773
Size: 500 879 492 298 1928
Fragments arranged by size:
1928 879 500 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 823 1611 3648 6
Size: 817 788 2037 455
Fragments arranged by size:
2037 817 788 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 687 687
Size: 4097
BseYI C'CCAG_C
Cuts at: 765 889 1522 2280 2857 3353 765
Size: 124 633 758 577 496 1509
Fragments arranged by size:
1509 758 633 577 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 168
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1053 1111 1889 2079 2871 1053
Size: 58 778 190 792 2279
Fragments arranged by size:
2279 792 778 190 58
Bsp1286I G_dGCh'C
Cuts at: 952 1006 1015 1053 1111 1722 1815 1889 2079
Size: 54 9 38 58 611 93 74 190
Cuts at: 2079 2141 2871 952
Size: 62 730 2178
Fragments arranged by size:
2178 730 611 190 93 74 62 58
54 38 9
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 656 3273 656
Size: 2617 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 124 348 1018 1147 1361 1663 2044 124
Size: 224 670 129 214 302 381 2177
Fragments arranged by size:
2177 670 381 302 224 214 129
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 352 2009 352
Size: 1657 2440
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 459 616 2458 459
Size: 157 1842 2098
Fragments arranged by size:
2098 1842 157
BstEII G'GTnAC_C
Cuts at: 985 985
Size: 4097
BstYI r'GATC_y
Cuts at: 1 1122 1589 1612 1947 2193 3205 3291 3303
Size: 1121 467 23 335 246 1012 86 12
Cuts at: 3303 3649 1
Size: 346 449
Fragments arranged by size:
1121 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 998 998
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
ClaI AT'CG_AT
Cuts at: 971 971
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 255 549 1682 1856 2247 2274 3619 255
Size: 294 1133 174 391 27 1345 733
Fragments arranged by size:
1345 1133 733 391 294 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 315 2120 2330 315
Size: 1805 210 2082
Fragments arranged by size:
2082 1805 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1051 1109 1051
Size: 58 4039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 452 977 1037 1922 2354 3101 3369 375
Size: 77 525 60 885 432 747 268 1103
Fragments arranged by size:
1103 885 747 525 432 268 77 60
EcoO109I rG'GnC_Cy
Cuts at: 913 931 949 994 1002 1003 1012 1021 1236
Size: 18 18 45 8 1 9 9 215
Cuts at: 1236 913
Size: 3774
Fragments arranged by size:
3774 215 45 18 18 9 9 8
1
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 1079 1531 1779 2801 3780 1079
Size: 452 248 1022 979 1396
Fragments arranged by size:
1396 1022 979 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 220 271 303 1939 1969 1971 2001 188
Size: 32 51 32 1636 30 2 30 2284
Fragments arranged by size:
2284 1636 51 32 32 30 30 2
HincII GTy'rAC
Cuts at: 696 1136 696
Size: 440 3657
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MfeI C'AATT_G
Cuts at: 740 740
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 2768 2952 424
Size: 2344 184 1569
Fragments arranged by size:
2344 1569 184
MscI TGG'CCA
Cuts at: 257 551 1858 257
Size: 294 1307 2496
Fragments arranged by size:
2496 1307 294
MslI CAynn'nnrTG
Cuts at: 2086 2213 2086
Size: 127 3970
MspA1I CmG'CkG
Cuts at: 178 1208 1522 1882 2895 3140 178
Size: 1030 314 360 1013 245 1135
Fragments arranged by size:
1135 1030 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PasI CC'CwG_GG
Cuts at: 164 164
Size: 4097
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PfoI T'CCnGG_A
Cuts at: 155 155
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 913 931 994 1021 913
Size: 18 63 27 3989
Fragments arranged by size:
3989 63 27 18
PshAI GACnn'nnGTC
Cuts at: 992 992
Size: 4097
PspOMI G'GGCC_C
Cuts at: 948 1002 1011 948
Size: 54 9 4034
Fragments arranged by size:
4034 54 9
PstI C_TGCA'G
Cuts at: 177 1029 1144 1829 177
Size: 852 115 685 2445
Fragments arranged by size:
2445 852 685 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 178 1522 1882 178
Size: 1344 360 2393
Fragments arranged by size:
2393 1344 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1053 1111 1053
Size: 58 4039
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 315 2120 2330 315
Size: 1805 210 2082
Fragments arranged by size:
2082 1805 210
SbfI CC_TGCA'GG
Cuts at: 1029 1144 1029
Size: 115 3982
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 173 1025 1140 1825 2818 3009 33
Size: 140 852 115 685 993 191 1121
Fragments arranged by size:
1121 993 852 685 191 140 115
SfiI GGCCn_nnn'nGGCC
Cuts at: 1010 1010
Size: 4097
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 920 939 1010 1019 1119 920
Size: 19 71 9 100 3898
Fragments arranged by size:
3898 100 71 19 9
SmlI C'TyrA_G
Cuts at: 788 1252 2167 2659 2921 788
Size: 464 915 492 262 1964
Fragments arranged by size:
1964 915 492 464 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 1231 1585 1231
Size: 354 3743
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1004 1680 1813 2272 2481 1004
Size: 676 133 459 209 2620
Fragments arranged by size:
2620 676 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 316 1128 316
Size: 812 3285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 918 937 1008 1017 1117 918
Size: 19 71 9 100 3898
Fragments arranged by size:
3898 100 71 19 9
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I BtgI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PsiI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI