Restriction map for
pF1KE6682
(Circular) (Six-base) MAPSORT of: pF1KE6682.seq Check: 6886 from: 1 to: 4055
pF1KE6682 4055 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1880 2312 3059 3327 689
Size: 1191 432 747 268 1417
Fragments arranged by size:
1417 1191 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 462 462
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 425 501 520 691 787 1059 3408 3664 425
Size: 76 19 171 96 272 2349 256 816
Fragments arranged by size:
2349 816 272 256 171 96 76 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 1071 1485 1733 1768 1071
Size: 414 248 35 3358
Fragments arranged by size:
3358 414 248 35
BanII G_rGCy'C
Cuts at: 1069 2099 1069
Size: 1030 3025
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BbsI GAAGACnn'nnnn_
Cuts at: 989 989
Size: 4055
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 279 1575 279
Size: 1296 2759
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2829 1680
Size: 93 1056 2906
Fragments arranged by size:
2906 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 156 1267 1348 1683 3827 156
Size: 1111 81 335 2144 384
Fragments arranged by size:
2144 1111 384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 4055
BseYI C'CCAG_C
Cuts at: 601 731 1480 2238 2815 3311 601
Size: 130 749 758 577 496 1345
Fragments arranged by size:
1345 758 749 577 496 130
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 558 1069 1847 2037 2829 558
Size: 511 778 190 792 1784
Fragments arranged by size:
1784 792 778 511 190
Bsp1286I G_dGCh'C
Cuts at: 558 1069 1680 1773 1847 2037 2099 2829 558
Size: 511 611 93 74 190 62 730 1784
Fragments arranged by size:
1784 730 611 511 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 390 3231 390
Size: 2841 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 142 2053 2234 2443 142
Size: 1911 181 209 1754
Fragments arranged by size:
1911 1754 209 181
BsrGI T'GTAC_A
Cuts at: 302 302
Size: 4055
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstEII G'GTnAC_C
Cuts at: 654 654
Size: 4055
BstYI r'GATC_y
Cuts at: 1 914 1080 1547 1570 1905 2151 3163 3249
Size: 913 166 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 913 467 449 346 335 246 166
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 737 1298 737
Size: 561 3494
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 1640 1814 2205 2232 3577 1640
Size: 174 391 27 1345 2118
Fragments arranged by size:
2118 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 617 756 2078 2288 617
Size: 139 1322 210 2384
Fragments arranged by size:
2384 1322 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 689 1880 2312 3059 3327 157
Size: 532 1191 432 747 268 885
Fragments arranged by size:
1191 885 747 532 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 172 2074 172
Size: 1902 2153
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 906 938 1897 1927 1929 1959 906
Size: 32 959 30 2 30 3002
Fragments arranged by size:
3002 959 32 30 30 2
HincII GTy'rAC
Cuts at: 800 1094 800
Size: 294 3761
HindIII A'AGCT_T
Cuts at: 154 154
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 592 2726 2910 167
Size: 425 2134 184 1312
Fragments arranged by size:
2134 1312 425 184
MscI TGG'CCA
Cuts at: 1816 1816
Size: 4055
MslI CAynn'nnrTG
Cuts at: 2044 2171 2044
Size: 127 3928
MspA1I CmG'CkG
Cuts at: 886 1166 1480 1840 2853 3098 886
Size: 280 314 360 1013 245 1843
Fragments arranged by size:
1843 1013 360 314 280 245
NaeI GCC'GGC
Cuts at: 144 2236 144
Size: 2092 1963
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 142 2234 142
Size: 2092 1963
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PsiI TTA'TAA
Cuts at: 727 727
Size: 4055
PstI C_TGCA'G
Cuts at: 451 680 891 1102 1787 451
Size: 229 211 211 685 2719
Fragments arranged by size:
2719 685 229 211 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 886 1480 1840 886
Size: 594 360 3101
Fragments arranged by size:
3101 594 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 617 2078 2288 617
Size: 1461 210 2384
Fragments arranged by size:
2384 1461 210
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 447 676 887 1098 1783 2776 2967 33
Size: 414 229 211 211 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 229 211 211 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1210 2125 2617 2879 1210
Size: 915 492 262 2386
Fragments arranged by size:
2386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
SspI AAT'ATT
Cuts at: 276 276
Size: 4055
StyI C'CwwG_G
Cuts at: 107 1189 1543 107
Size: 1082 354 2619
Fragments arranged by size:
2619 1082 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1638 1771 2230 2439 1638
Size: 133 459 209 3254
Fragments arranged by size:
3254 459 209 133
TatI w'GTAC_w
Cuts at: 302 3570 302
Size: 3268 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 576 1086 576
Size: 510 3545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI