Restriction map for
pF1KE6687
(Circular) (Six-base) MAPSORT of: pF1KE6687.seq Check: 8476 from: 1 to: 3809
pF1KE6687 3809 bp
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AccI GT'mk_AC
Cuts at: 847 847
Size: 3809
Acc65I G'GTAC_C
Cuts at: 825 825
Size: 3809
AclI AA'CG_TT
Cuts at: 3649 3649
Size: 3809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 1634 2066 2813 3081 627
Size: 1007 432 747 268 1355
Fragments arranged by size:
1355 1007 747 432 268
AfeI AGC'GCT
Cuts at: 115 652 748 115
Size: 537 96 3176
Fragments arranged by size:
3176 537 96
AflIII A'CryG_T
Cuts at: 584 1161 2265 3242 584
Size: 577 1104 977 1151
Fragments arranged by size:
1151 1104 977 577
AgeI A'CCGG_T
Cuts at: 2197 2197
Size: 3809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 3809
AlwNI CAG_nnn'CTG
Cuts at: 429 1114 2681 429
Size: 685 1567 1557
Fragments arranged by size:
1567 1557 685
ApaLI G'TGCA_C
Cuts at: 700 2579 700
Size: 1879 1930
ApoI r'AATT_y
Cuts at: 813 3162 3418 813
Size: 2349 256 1204
Fragments arranged by size:
2349 1204 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
AvaI C'yCGr_G
Cuts at: 316 358 667 829 316
Size: 42 309 162 3296
Fragments arranged by size:
3296 309 162 42
AvrII C'CTAG_G
Cuts at: 126 126
Size: 3809
BamHI G'GATC_C
Cuts at: 834 834
Size: 3809
BanI G'GyrC_C
Cuts at: 213 825 1239 1487 1522 213
Size: 612 414 248 35 2500
Fragments arranged by size:
2500 612 414 248 35
BanII G_rGCy'C
Cuts at: 823 1853 823
Size: 1030 2779
BbeI G_GCGC'C
Cuts at: 1243 1491 1243
Size: 248 3561
BciVI GTATCCnnnnn_n'
Cuts at: 1707 2468 3809 1707
Size: 761 1341 1707
Fragments arranged by size:
1707 1341 761
BclI T'GATC_A
Cuts at: 1329 1329
Size: 3809
BglII A'GATC_T
Cuts at: 1324 3361 1324
Size: 2037 1772
BlpI GC'TnA_GC
Cuts at: 921 921
Size: 3809
Bme1580I G_kGCm'C
Cuts at: 668 704 1434 1527 2583 668
Size: 36 730 93 1056 1894
Fragments arranged by size:
1894 1056 730 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 1021 1102 1437 3581 1021
Size: 81 335 2144 1249
Fragments arranged by size:
2144 1249 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 3809
Bpu10I CC'TnA_GC
Cuts at: 517 517
Size: 3809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1864 2356 2654 985
Size: 879 492 298 2140
Fragments arranged by size:
2140 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3513 3513
Size: 3809
BsaAI yAC'GTr
Cuts at: 624 1162 624
Size: 538 3271
BsaBI GATnn'nnATC
Cuts at: 6 763 1323 3360 6
Size: 757 560 2037 455
Fragments arranged by size:
2037 757 560 455
BsaHI Gr'CG_yC
Cuts at: 1240 1488 2190 3686 1240
Size: 248 702 1496 1363
Fragments arranged by size:
1496 1363 702 248
BsaWI w'CCGG_w
Cuts at: 998 1519 2197 2471 2618 3029 998
Size: 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 121 217 391 1234 1992 2569 3065 121
Size: 96 174 843 758 577 496 865
Fragments arranged by size:
865 843 758 577 496 174 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 407
Size: 3809
BsiEI CG_ry'CG
Cuts at: 104 182 442 1351 1397 2605 104
Size: 78 260 909 46 1208 1308
Fragments arranged by size:
1308 1208 909 260 78 46
BsiHKAI G_wGCw'C
Cuts at: 661 704 799 823 1601 1791 2583 661
Size: 43 95 24 778 190 792 1887
Fragments arranged by size:
1887 792 778 190 95 43 24
BsmBI CGTCTCn'nnnn_
Cuts at: 289 289
Size: 3809
Bsp1286I G_dGCh'C
Cuts at: 661 668 704 799 823 1434 1527 1601 1791
Size: 7 36 95 24 611 93 74 190
Cuts at: 1791 1853 2583 661
Size: 62 730 1887
Fragments arranged by size:
1887 730 611 190 95 93 74 62
36 24 7
BspEI T'CCGG_A
Cuts at: 3029 3029
Size: 3809
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 859 1073 1375 1756 236
Size: 623 214 302 381 2289
Fragments arranged by size:
2289 623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2101 2155 3639 3804 50
Size: 2051 54 1484 165 55
Fragments arranged by size:
2051 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1721 1721
Size: 3809
BsrFI r'CCGG_y
Cuts at: 177 181 365 1807 1988 2197 177
Size: 4 184 1442 181 209 1789
Fragments arranged by size:
1789 1442 209 184 181 4
BssSI C'ACGA_G
Cuts at: 654 798 2080 2438 654
Size: 144 1282 358 2025
Fragments arranged by size:
2025 1282 358 144
BstAPI GCAn_nnn'nTGC
Cuts at: 680 1186 680
Size: 506 3303
BstBI TT'CG_AA
Cuts at: 2170 2170
Size: 3809
BstEII G'GTnAC_C
Cuts at: 132 132
Size: 3809
BstYI r'GATC_y
Cuts at: 1 285 834 1301 1324 1659 1905 2917 3003
Size: 284 549 467 23 335 246 1012 86
Cuts at: 3003 3015 3361 1
Size: 12 346 449
Fragments arranged by size:
1012 549 467 449 346 335 284 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1759 1940 2103 1759
Size: 181 163 3465
Fragments arranged by size:
3465 181 163
BtsI GCAGTG_nn'
Cuts at: 1052 1052
Size: 3809
DraI TTT'AAA
Cuts at: 808 808
Size: 3809
DrdI GACnn_nn'nnGTC
Cuts at: 1515 2373 1515
Size: 858 2951
EaeI y'GGCC_r
Cuts at: 179 186 344 439 613 1394 1568 1959 1986
Size: 7 158 95 174 781 174 391 27
Cuts at: 1986 3331 179
Size: 1345 657
Fragments arranged by size:
1345 781 657 391 174 174 158 95
27 7
EagI C'GGCC_G
Cuts at: 179 439 1394 179
Size: 260 955 2594
Fragments arranged by size:
2594 955 260
EarI CTCTTCn'nnn_
Cuts at: 1832 2042 1832
Size: 210 3599
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 427 2017 2327 2473 3619 196
Size: 231 1590 310 146 1146 386
Fragments arranged by size:
1590 1146 386 310 231 146
EcoICRI GAG'CTC
Cuts at: 821 821
Size: 3809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 1634 2066 2813 3081 627
Size: 1007 432 747 268 1355
Fragments arranged by size:
1355 1007 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 405 948 405
Size: 543 3266
EcoRI G'AATT_C
Cuts at: 813 813
Size: 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3809
FspI TGC'GCA
Cuts at: 1590 1590
Size: 3809
HaeII r_GCGC'y
Cuts at: 117 516 654 726 750 1243 1491 2513 3492
Size: 399 138 72 24 493 248 1022 979
Cuts at: 3492 117
Size: 434
Fragments arranged by size:
1022 979 493 434 399 248 138 72
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1651 1681 1683 1713 1651
Size: 30 2 30 3747
Fragments arranged by size:
3747 30 30 2
HincII GTy'rAC
Cuts at: 848 848
Size: 3809
KasI G'GCGC_C
Cuts at: 1239 1487 1239
Size: 248 3561
KpnI G_GTAC'C
Cuts at: 829 829
Size: 3809
MluI A'CGCG_T
Cuts at: 584 3242 584
Size: 2658 1151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2664 2480
Size: 184 3625
MscI TGG'CCA
Cuts at: 346 1570 346
Size: 1224 2585
MslI CAynn'nnrTG
Cuts at: 1798 1925 1798
Size: 127 3682
MspA1I CmG'CkG
Cuts at: 391 426 438 638 732 920 1234 1594 2607
Size: 35 12 200 94 188 314 360 1013
Cuts at: 2607 2852 391
Size: 245 1348
Fragments arranged by size:
1348 1013 360 314 245 200 188 94
35 12
NaeI GCC'GGC
Cuts at: 183 1990 183
Size: 1807 2002
NarI GG'CG_CC
Cuts at: 1240 1488 1240
Size: 248 3561
NcoI C'CATG_G
Cuts at: 107 107
Size: 3809
NgoMIV G'CCGG_C
Cuts at: 181 1988 181
Size: 1807 2002
NspI r_CATG'y
Cuts at: 862 1362 2269 862
Size: 500 907 2402
Fragments arranged by size:
2402 907 500
PasI CC'CwG_GG
Cuts at: 498 498
Size: 3809
PciI A'CATG_T
Cuts at: 2265 2265
Size: 3809
PmeI GTTT'AAAC
Cuts at: 808 808
Size: 3809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3777
PpuMI rG'GwC_Cy
Cuts at: 405 405
Size: 3809
PstI C_TGCA'G
Cuts at: 856 1541 856
Size: 685 3124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3809
PvuII CAG'CTG
Cuts at: 391 426 638 1234 1594 391
Size: 35 212 596 360 2606
Fragments arranged by size:
2606 596 360 212 35
RsrII CG'GwC_CG
Cuts at: 2004 2004
Size: 3809
SacI G_AGCT'C
Cuts at: 823 823
Size: 3809
SalI G'TCGA_C
Cuts at: 846 846
Size: 3809
SapI GCTCTTCn'nnn_
Cuts at: 1832 2042 1832
Size: 210 3599
SbfI CC_TGCA'GG
Cuts at: 856 856
Size: 3809
ScaI AGT'ACT
Cuts at: 3326 3326
Size: 3809
SfcI C'TryA_G
Cuts at: 33 852 1537 2530 2721 33
Size: 819 685 993 191 1121
Fragments arranged by size:
1121 993 819 685 191
SfoI GGC'GCC
Cuts at: 1241 1489 1241
Size: 248 3561
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
SmaI CCC'GGG
Cuts at: 831 831
Size: 3809
SmlI C'TyrA_G
Cuts at: 964 1879 2371 2633 964
Size: 915 492 262 2140
Fragments arranged by size:
2140 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3809
SphI G_CATG'C
Cuts at: 862 1362 862
Size: 500 3309
StyI C'CwwG_G
Cuts at: 107 126 275 943 1297 107
Size: 19 149 668 354 2619
Fragments arranged by size:
2619 668 354 149 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 231 269 1392 1525 1984 2193 231
Size: 38 1123 133 459 209 1847
Fragments arranged by size:
1847 1123 459 209 133 38
TatI w'GTAC_w
Cuts at: 3324 3324
Size: 3809
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 3777
Tth111I GACn'n_nGTC
Cuts at: 1606 1606
Size: 3809
XbaI T'CTAG_A
Cuts at: 840 840
Size: 3809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1304
Size: 3809
XmaI C'CCGG_G
Cuts at: 829 829
Size: 3809
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI