Restriction map for
pF1KE6787
(Circular) (Six-base) MAPSORT of: pF1KE6787.seq Check: 9866 from: 1 to: 5186
pF1KE6787 5186 bp
With 182 enzymes: SgfI *
August 9, 2016 17:39 ..
AccI GT'mk_AC
Cuts at: 2224 2224
Size: 5186
Acc65I G'GTAC_C
Cuts at: 2202 2202
Size: 5186
AclI AA'CG_TT
Cuts at: 5026 5026
Size: 5186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3443 4190 4458 3011
Size: 432 747 268 3739
Fragments arranged by size:
3739 747 432 268
AfeI AGC'GCT
Cuts at: 1265 1265
Size: 5186
AflII C'TTAA_G
Cuts at: 1884 1884
Size: 5186
AflIII A'CryG_T
Cuts at: 2538 3642 4619 2538
Size: 1104 977 3105
Fragments arranged by size:
3105 1104 977
AgeI A'CCGG_T
Cuts at: 3574 3574
Size: 5186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1781 1781
Size: 5186
AlwNI CAG_nnn'CTG
Cuts at: 1536 2017 2491 4058 1536
Size: 481 474 1567 2664
Fragments arranged by size:
2664 1567 481 474
ApaLI G'TGCA_C
Cuts at: 3956 3956
Size: 5186
ApoI r'AATT_y
Cuts at: 408 529 1408 1923 2190 4539 4795 408
Size: 121 879 515 267 2349 256 799
Fragments arranged by size:
2349 879 799 515 267 256 121
AseI AT'TA_AT
Cuts at: 20 1459 20
Size: 1439 3747
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
AvaI C'yCGr_G
Cuts at: 2206 2206
Size: 5186
AvrII C'CTAG_G
Cuts at: 2127 2127
Size: 5186
BamHI G'GATC_C
Cuts at: 2211 2211
Size: 5186
BanI G'GyrC_C
Cuts at: 141 326 2202 2616 2864 2899 141
Size: 185 1876 414 248 35 2428
Fragments arranged by size:
2428 1876 414 248 185 35
BanII G_rGCy'C
Cuts at: 1249 2200 3230 1249
Size: 951 1030 3205
Fragments arranged by size:
3205 1030 951
BbeI G_GCGC'C
Cuts at: 2620 2868 2620
Size: 248 4938
BbsI GAAGACnn'nnnn_
Cuts at: 1912 1912
Size: 5186
BbvCI CC'TCA_GC
Cuts at: 195 1141 195
Size: 946 4240
BciVI GTATCCnnnnn_n'
Cuts at: 144 3084 3845 5186 144
Size: 2940 761 1341 144
Fragments arranged by size:
2940 1341 761 144
BclI T'GATC_A
Cuts at: 740 960 2706 740
Size: 220 1746 3220
Fragments arranged by size:
3220 1746 220
BglII A'GATC_T
Cuts at: 488 2701 4738 488
Size: 2213 2037 936
Fragments arranged by size:
2213 2037 936
BlpI GC'TnA_GC
Cuts at: 2298 2298
Size: 5186
Bme1580I G_kGCm'C
Cuts at: 331 1556 2811 2904 3960 331
Size: 1225 1255 93 1056 1557
Fragments arranged by size:
1557 1255 1225 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 332 1252 2398 2479 2814 4958 332
Size: 920 1146 81 335 2144 560
Fragments arranged by size:
2144 1146 920 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 467 3553 467
Size: 3086 2100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 742 1219 422
Size: 320 477 4389
Fragments arranged by size:
4389 477 320
Bpu10I CC'TnA_GC
Cuts at: 195 1141 195
Size: 946 4240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 2362 3241 3733 4031 273
Size: 2089 879 492 298 1428
Fragments arranged by size:
2089 1428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4890 4890
Size: 5186
BsaAI yAC'GTr
Cuts at: 1557 2539 1557
Size: 982 4204
BsaBI GATnn'nnATC
Cuts at: 6 362 1834 2700 4737 6
Size: 356 1472 866 2037 455
Fragments arranged by size:
2037 1472 866 455 356
BsaHI Gr'CG_yC
Cuts at: 2617 2865 3567 5063 2617
Size: 248 702 1496 2740
Fragments arranged by size:
2740 1496 702 248
BsaWI w'CCGG_w
Cuts at: 116 2375 2896 3574 3848 3995 4406 116
Size: 2259 521 678 274 147 411 896
Fragments arranged by size:
2259 896 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 351 862 1129 1303 351
Size: 511 267 174 4234
Fragments arranged by size:
4234 511 267 174
BseYI C'CCAG_C
Cuts at: 1254 2020 2611 3369 3946 4442 1254
Size: 766 591 758 577 496 1998
Fragments arranged by size:
1998 766 758 591 577 496
BsiEI CG_ry'CG
Cuts at: 104 2728 2774 3982 104
Size: 2624 46 1208 1308
Fragments arranged by size:
2624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1883 2200 2978 3168 3960 1883
Size: 317 778 190 792 3109
Fragments arranged by size:
3109 792 778 317 190
BsiWI C'GTAC_G
Cuts at: 835 835
Size: 5186
BsmI GAATG_Cn'
Cuts at: 353 353
Size: 5186
Bsp1286I G_dGCh'C
Cuts at: 331 1249 1556 1883 2200 2811 2904 2978 3168
Size: 918 307 327 317 611 93 74 190
Cuts at: 3168 3230 3960 331
Size: 62 730 1557
Fragments arranged by size:
1557 918 730 611 327 317 307 190
93 74 62
BspEI T'CCGG_A
Cuts at: 116 4406 116
Size: 4290 896
BspHI T'CATG_A
Cuts at: 1733 1845 4362 1733
Size: 112 2517 2557
Fragments arranged by size:
2557 2517 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 1042 2236 2450 2752 3133 251
Size: 791 1194 214 302 381 2304
Fragments arranged by size:
2304 1194 791 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3478 3532 5016 5181 50
Size: 3428 54 1484 165 55
Fragments arranged by size:
3428 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3098 3098
Size: 5186
BsrFI r'CCGG_y
Cuts at: 234 3184 3365 3574 234
Size: 2950 181 209 1846
Fragments arranged by size:
2950 1846 209 181
BssSI C'ACGA_G
Cuts at: 3457 3815 3457
Size: 358 4828
BstAPI GCAn_nnn'nTGC
Cuts at: 368 2563 368
Size: 2195 2991
BstBI TT'CG_AA
Cuts at: 3547 3547
Size: 5186
BstEII G'GTnAC_C
Cuts at: 265 265
Size: 5186
BstXI CCAn_nnnn'nTGG
Cuts at: 1022 1022
Size: 5186
BstYI r'GATC_y
Cuts at: 1 488 2003 2211 2678 2701 3036 3282 4294
Size: 487 1515 208 467 23 335 246 1012
Cuts at: 4294 4380 4392 4738 1
Size: 86 12 346 449
Fragments arranged by size:
1515 1012 487 467 449 346 335 246
208 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 556 1373 556
Size: 817 4369
BtgI C'CryG_G
Cuts at: 216 331 989 1473 216
Size: 115 658 484 3929
Fragments arranged by size:
3929 658 484 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3136 3317 3480 3136
Size: 181 163 4842
Fragments arranged by size:
4842 181 163
BtsI GCAGTG_nn'
Cuts at: 702 2015 2429 702
Size: 1313 414 3459
Fragments arranged by size:
3459 1313 414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1812 1847 1812
Size: 35 5151
DraI TTT'AAA
Cuts at: 2185 2185
Size: 5186
DrdI GACnn_nn'nnGTC
Cuts at: 2892 3750 2892
Size: 858 4328
EaeI y'GGCC_r
Cuts at: 1328 2771 2945 3336 3363 4708 1328
Size: 1443 174 391 27 1345 1806
Fragments arranged by size:
1806 1443 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2771 2771
Size: 5186
EarI CTCTTCn'nnn_
Cuts at: 458 479 987 3209 3419 458
Size: 21 508 2222 210 2225
Fragments arranged by size:
2225 2222 508 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 838 3394 3704 3850 4996 838
Size: 2556 310 146 1146 1028
Fragments arranged by size:
2556 1146 1028 310 146
EcoICRI GAG'CTC
Cuts at: 2198 2198
Size: 5186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 742 1219 3011 3443 4190 4458 422
Size: 320 477 1792 432 747 268 1150
Fragments arranged by size:
1792 1150 747 477 432 320 268
EcoNI CCTnn'n_nnAGG
Cuts at: 247 559 247
Size: 312 4874
EcoO109I rG'GnC_Cy
Cuts at: 1077 1533 2325 1077
Size: 456 792 3938
Fragments arranged by size:
3938 792 456
EcoRI G'AATT_C
Cuts at: 2190 2190
Size: 5186
EcoRV GAT'ATC
Cuts at: 606 606
Size: 5186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 439 1119 3205 439
Size: 680 2086 2420
Fragments arranged by size:
2420 2086 680
FspI TGC'GCA
Cuts at: 2967 2967
Size: 5186
HaeII r_GCGC'y
Cuts at: 1267 2620 2868 3890 4869 1267
Size: 1353 248 1022 979 1584
Fragments arranged by size:
1584 1353 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 3028 3058 3060 3090 178
Size: 32 2818 30 2 30 2274
Fragments arranged by size:
2818 2274 32 30 30 2
HincII GTy'rAC
Cuts at: 2225 2225
Size: 5186
KasI G'GCGC_C
Cuts at: 2616 2864 2616
Size: 248 4938
KpnI G_GTAC'C
Cuts at: 2206 2206
Size: 5186
MluI A'CGCG_T
Cuts at: 4619 4619
Size: 5186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 293 1721 3857 4041 293
Size: 1428 2136 184 1438
Fragments arranged by size:
2136 1438 1428 184
MscI TGG'CCA
Cuts at: 2947 2947
Size: 5186
MslI CAynn'nnrTG
Cuts at: 1283 1421 1918 3175 3302 1283
Size: 138 497 1257 127 3167
Fragments arranged by size:
3167 1257 497 138 127
MspA1I CmG'CkG
Cuts at: 1053 1145 1314 1666 1981 2297 2611 2971 3984
Size: 92 169 352 315 316 314 360 1013
Cuts at: 3984 4229 1053
Size: 245 2010
Fragments arranged by size:
2010 1013 360 352 316 315 314 245
169 92
NaeI GCC'GGC
Cuts at: 3367 3367
Size: 5186
NarI GG'CG_CC
Cuts at: 2617 2865 2617
Size: 248 4938
NcoI C'CATG_G
Cuts at: 989 1473 989
Size: 484 4702
NgoMIV G'CCGG_C
Cuts at: 3365 3365
Size: 5186
NspI r_CATG'y
Cuts at: 2239 2739 3646 2239
Size: 500 907 3779
Fragments arranged by size:
3779 907 500
PciI A'CATG_T
Cuts at: 3642 3642
Size: 5186
PflMI CCAn_nnn'nTGG
Cuts at: 654 1411 654
Size: 757 4429
PmeI GTTT'AAAC
Cuts at: 2185 2185
Size: 5186
PmlI CAC'GTG
Cuts at: 1557 1557
Size: 5186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 4325
Size: 32 5154
PsiI TTA'TAA
Cuts at: 1930 1930
Size: 5186
PstI C_TGCA'G
Cuts at: 674 2233 2918 674
Size: 1559 685 2942
Fragments arranged by size:
2942 1559 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5186
PvuII CAG'CTG
Cuts at: 1053 1145 1314 1666 1981 2611 2971 1053
Size: 92 169 352 315 630 360 3268
Fragments arranged by size:
3268 630 360 352 315 169 92
RsrII CG'GwC_CG
Cuts at: 3381 3381
Size: 5186
SacI G_AGCT'C
Cuts at: 2200 2200
Size: 5186
SalI G'TCGA_C
Cuts at: 2223 2223
Size: 5186
SapI GCTCTTCn'nnn_
Cuts at: 3209 3419 3209
Size: 210 4976
SbfI CC_TGCA'GG
Cuts at: 2233 2233
Size: 5186
ScaI AGT'ACT
Cuts at: 628 897 4703 628
Size: 269 3806 1111
Fragments arranged by size:
3806 1111 269
SfcI C'TryA_G
Cuts at: 33 247 670 1069 1212 1296 1676 2229 2914
Size: 214 423 399 143 84 380 553 685
Cuts at: 2914 3907 4098 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 553 423 399 380 214
191 143 84
SfoI GGC'GCC
Cuts at: 2618 2866 2618
Size: 248 4938
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
SmaI CCC'GGG
Cuts at: 2208 2208
Size: 5186
SmlI C'TyrA_G
Cuts at: 288 1884 2341 3256 3748 4010 288
Size: 1596 457 915 492 262 1464
Fragments arranged by size:
1596 1464 915 492 457 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5186
SphI G_CATG'C
Cuts at: 2239 2739 2239
Size: 500 4686
SspI AAT'ATT
Cuts at: 2172 2172
Size: 5186
StyI C'CwwG_G
Cuts at: 989 1163 1473 2042 2127 2320 2674 989
Size: 174 310 569 85 193 354 3501
Fragments arranged by size:
3501 569 354 310 193 174 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 287 314 2769 2902 3361 3570 129
Size: 158 27 2455 133 459 209 1745
Fragments arranged by size:
2455 1745 459 209 158 133 27
TatI w'GTAC_w
Cuts at: 626 895 4701 626
Size: 269 3806 1111
Fragments arranged by size:
3806 1111 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4703 4735 4703
Size: 32 5154
Tth111I GACn'n_nGTC
Cuts at: 1341 2983 1341
Size: 1642 3544
XbaI T'CTAG_A
Cuts at: 2217 2217
Size: 5186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2681 2681
Size: 5186
XmaI C'CCGG_G
Cuts at: 2206 2206
Size: 5186
XmnI GAAnn'nnTTC
Cuts at: 2054 2054
Size: 5186
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI BaeI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI BsmBI
BsrGI BssHII BstZ17I ClaI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI