Restriction map for
pF1KE8021
(Circular) (Six-base) MAPSORT of: pF1KE8021.seq Check: 9032 from: 1 to: 5336
pF1KE8021 5336 bp
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AccI GT'mk_AC
Cuts at: 796 2374 796
Size: 1578 3758
Acc65I G'GTAC_C
Cuts at: 2352 2352
Size: 5336
AclI AA'CG_TT
Cuts at: 5176 5176
Size: 5336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 1777 1802 2281 3161 3593 4340 4608 1037
Size: 740 25 479 880 432 747 268 1765
Fragments arranged by size:
1765 880 747 740 479 432 268 25
AflIII A'CryG_T
Cuts at: 2688 3792 4769 2688
Size: 1104 977 3255
Fragments arranged by size:
3255 1104 977
AgeI A'CCGG_T
Cuts at: 126 3724 126
Size: 3598 1738
AleI CACnn'nnGTG
Cuts at: 736 1816 736
Size: 1080 4256
AlwNI CAG_nnn'CTG
Cuts at: 273 2641 4208 273
Size: 2368 1567 1401
Fragments arranged by size:
2368 1567 1401
ApaI G_GGCC'C
Cuts at: 364 364
Size: 5336
ApaLI G'TGCA_C
Cuts at: 4106 4106
Size: 5336
ApoI r'AATT_y
Cuts at: 534 541 701 1384 2340 4689 4945 534
Size: 7 160 683 956 2349 256 925
Fragments arranged by size:
2349 956 925 683 256 160 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 5336
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
AvaI C'yCGr_G
Cuts at: 1631 2356 1631
Size: 725 4611
BamHI G'GATC_C
Cuts at: 660 2361 660
Size: 1701 3635
BanI G'GyrC_C
Cuts at: 2352 2766 3014 3049 2352
Size: 414 248 35 4639
Fragments arranged by size:
4639 414 248 35
BanII G_rGCy'C
Cuts at: 364 386 2060 2350 3380 364
Size: 22 1674 290 1030 2320
Fragments arranged by size:
2320 1674 1030 290 22
BbeI G_GCGC'C
Cuts at: 2770 3018 2770
Size: 248 5088
BbsI GAAGACnn'nnnn_
Cuts at: 335 2194 335
Size: 1859 3477
BbvCI CC'TCA_GC
Cuts at: 1801 1801
Size: 5336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1461 1495 1461
Size: 34 5302
BciVI GTATCCnnnnn_n'
Cuts at: 653 3234 3995 5336 653
Size: 2581 761 1341 653
Fragments arranged by size:
2581 1341 761 653
BclI T'GATC_A
Cuts at: 2115 2856 2115
Size: 741 4595
BfrBI ATG'CAT
Cuts at: 1005 1005
Size: 5336
BglI GCCn_nnn'nGGC
Cuts at: 112 359 368 1488 112
Size: 247 9 1120 3960
Fragments arranged by size:
3960 1120 247 9
BglII A'GATC_T
Cuts at: 1917 2851 4888 1917
Size: 934 2037 2365
Fragments arranged by size:
2365 2037 934
BlpI GC'TnA_GC
Cuts at: 1964 2448 1964
Size: 484 4852
Bme1580I G_kGCm'C
Cuts at: 364 2961 3054 4110 364
Size: 2597 93 1056 1590
Fragments arranged by size:
2597 1590 1056 93
BmgBI CAC'GTC
Cuts at: 171 171
Size: 5336
BmrI ACTGGGnnnn_n'
Cuts at: 390 2056 2548 2629 2964 5108 390
Size: 1666 492 81 335 2144 618
Fragments arranged by size:
2144 1666 618 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3703 3703
Size: 5336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1265 566
Size: 699 4637
Bpu10I CC'TnA_GC
Cuts at: 1801 1801
Size: 5336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2512 3391 3883 4181 2512
Size: 879 492 298 3667
Fragments arranged by size:
3667 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5040 5040
Size: 5336
BsaAI yAC'GTr
Cuts at: 669 715 2689 669
Size: 46 1974 3316
Fragments arranged by size:
3316 1974 46
BsaBI GATnn'nnATC
Cuts at: 6 1552 2850 4887 6
Size: 1546 1298 2037 455
Fragments arranged by size:
2037 1546 1298 455
BsaHI Gr'CG_yC
Cuts at: 2767 3015 3717 5213 2767
Size: 248 702 1496 2890
Fragments arranged by size:
2890 1496 702 248
BsaWI w'CCGG_w
Cuts at: 126 2525 3046 3724 3998 4145 4556 126
Size: 2399 521 678 274 147 411 906
Fragments arranged by size:
2399 906 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2179 2209 2179
Size: 30 5306
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1604 1766 1789 1876 2281 1604
Size: 162 23 87 405 4659
Fragments arranged by size:
4659 405 162 87 23
BseYI C'CCAG_C
Cuts at: 364 2761 3519 4096 4592 364
Size: 2397 758 577 496 1108
Fragments arranged by size:
2397 1108 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 2878 2924 4132 104
Size: 12 2762 46 1208 1308
Fragments arranged by size:
2762 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 2060 2350 3128 3318 4110 2060
Size: 290 778 190 792 3286
Fragments arranged by size:
3286 792 778 290 190
BsmI GAATG_Cn'
Cuts at: 1106 1576 1792 2246 1106
Size: 470 216 454 4196
Fragments arranged by size:
4196 470 454 216
BsmBI CGTCTCn'nnnn_
Cuts at: 1616 1616
Size: 5336
Bsp1286I G_dGCh'C
Cuts at: 364 386 2060 2350 2961 3054 3128 3318 3380
Size: 22 1674 290 611 93 74 190 62
Cuts at: 3380 4110 364
Size: 730 1590
Fragments arranged by size:
1674 1590 730 611 290 190 93 74
62 22
BspEI T'CCGG_A
Cuts at: 4556 4556
Size: 5336
BspHI T'CATG_A
Cuts at: 1332 2015 4512 1332
Size: 683 2497 2156
Fragments arranged by size:
2497 2156 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2386 2600 2902 3283 2386
Size: 214 302 381 4439
Fragments arranged by size:
4439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3628 3682 5166 5331 50
Size: 3578 54 1484 165 55
Fragments arranged by size:
3578 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 994 1477 2142 3248 994
Size: 483 665 1106 3082
Fragments arranged by size:
3082 1106 665 483
BsrFI r'CCGG_y
Cuts at: 126 3334 3515 3724 126
Size: 3208 181 209 1738
Fragments arranged by size:
3208 1738 209 181
BsrGI T'GTAC_A
Cuts at: 908 1344 908
Size: 436 4900
BssSI C'ACGA_G
Cuts at: 3607 3965 3607
Size: 358 4978
BstAPI GCAn_nnn'nTGC
Cuts at: 2713 2713
Size: 5336
BstBI TT'CG_AA
Cuts at: 3697 3697
Size: 5336
BstYI r'GATC_y
Cuts at: 1 660 1299 1917 2361 2828 2851 3186 3432
Size: 659 639 618 444 467 23 335 246
Cuts at: 3432 4444 4530 4542 4888 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 659 639 618 467 449 444 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 797 797
Size: 5336
BtgI C'CryG_G
Cuts at: 107 107
Size: 5336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3286 3467 3630 3286
Size: 181 163 4992
Fragments arranged by size:
4992 181 163
BtsI GCAGTG_nn'
Cuts at: 2579 2579
Size: 5336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 567 602 567
Size: 35 5301
DraI TTT'AAA
Cuts at: 2335 2335
Size: 5336
DraIII CAC_nnn'GTG
Cuts at: 174 672 715 174
Size: 498 43 4795
Fragments arranged by size:
4795 498 43
DrdI GACnn_nn'nnGTC
Cuts at: 3042 3900 3042
Size: 858 4478
EaeI y'GGCC_r
Cuts at: 113 1582 2921 3095 3486 3513 4858 113
Size: 1469 1339 174 391 27 1345 591
Fragments arranged by size:
1469 1345 1339 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2921 113
Size: 2808 2528
EarI CTCTTCn'nnn_
Cuts at: 374 755 1931 3359 3569 374
Size: 381 1176 1428 210 2141
Fragments arranged by size:
2141 1428 1176 381 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 317 3544 3854 4000 5146 317
Size: 3227 310 146 1146 507
Fragments arranged by size:
3227 1146 507 310 146
EcoICRI GAG'CTC
Cuts at: 2058 2348 2058
Size: 290 5046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1037 1265 1777 1802 2281 3161 3593 4340
Size: 471 228 512 25 479 880 432 747
Cuts at: 4340 4608 566
Size: 268 1294
Fragments arranged by size:
1294 880 747 512 479 471 432 268
228 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2263 2263
Size: 5336
EcoO109I rG'GnC_Cy
Cuts at: 360 361 1537 2475 360
Size: 1 1176 938 3221
Fragments arranged by size:
3221 1176 938 1
EcoRI G'AATT_C
Cuts at: 534 2340 534
Size: 1806 3530
EcoRV GAT'ATC
Cuts at: 1321 2013 2310 1321
Size: 692 297 4347
Fragments arranged by size:
4347 692 297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 3355 1807
Size: 1548 3788
FspI TGC'GCA
Cuts at: 3117 3117
Size: 5336
HaeII r_GCGC'y
Cuts at: 2770 3018 4040 5019 2770
Size: 248 1022 979 3087
Fragments arranged by size:
3087 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1135 1167 1932 1964 2100 2132 3178 3208 3210
Size: 32 765 32 136 32 1046 30 2
Cuts at: 3210 3240 1135
Size: 30 3231
Fragments arranged by size:
3231 1046 765 136 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 680 2375 680
Size: 1695 3641
HindIII A'AGCT_T
Cuts at: 1751 1751
Size: 5336
HpaI GTT'AAC
Cuts at: 680 680
Size: 5336
KasI G'GCGC_C
Cuts at: 2766 3014 2766
Size: 248 5088
KpnI G_GTAC'C
Cuts at: 2356 2356
Size: 5336
MluI A'CGCG_T
Cuts at: 4769 4769
Size: 5336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 238 1199 4007 4191 238
Size: 961 2808 184 1383
Fragments arranged by size:
2808 1383 961 184
MscI TGG'CCA
Cuts at: 3097 3097
Size: 5336
MslI CAynn'nnrTG
Cuts at: 736 1816 3325 3452 736
Size: 1080 1509 127 2620
Fragments arranged by size:
2620 1509 1080 127
MspA1I CmG'CkG
Cuts at: 368 1872 2106 2447 2761 3121 4134 4379 368
Size: 1504 234 341 314 360 1013 245 1325
Fragments arranged by size:
1504 1325 1013 360 341 314 245 234
NaeI GCC'GGC
Cuts at: 3517 3517
Size: 5336
NarI GG'CG_CC
Cuts at: 2767 3015 2767
Size: 248 5088
NcoI C'CATG_G
Cuts at: 107 107
Size: 5336
NdeI CA'TA_TG
Cuts at: 1579 1579
Size: 5336
NgoMIV G'CCGG_C
Cuts at: 3515 3515
Size: 5336
NotI GC'GGCC_GC
Cuts at: 113 113
Size: 5336
NsiI A_TGCA'T
Cuts at: 1007 1007
Size: 5336
NspI r_CATG'y
Cuts at: 634 2389 2889 3796 634
Size: 1755 500 907 2174
Fragments arranged by size:
2174 1755 907 500
PciI A'CATG_T
Cuts at: 3792 3792
Size: 5336
PfoI T'CCnGG_A
Cuts at: 188 656 1171 188
Size: 468 515 4353
Fragments arranged by size:
4353 515 468
PmeI GTTT'AAAC
Cuts at: 2335 2335
Size: 5336
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 4475
Size: 32 5304
PpuMI rG'GwC_Cy
Cuts at: 1537 1537
Size: 5336
PspOMI G'GGCC_C
Cuts at: 360 360
Size: 5336
PstI C_TGCA'G
Cuts at: 1489 2383 3068 1489
Size: 894 685 3757
Fragments arranged by size:
3757 894 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5336
PvuII CAG'CTG
Cuts at: 2106 2761 3121 2106
Size: 655 360 4321
Fragments arranged by size:
4321 655 360
RsrII CG'GwC_CG
Cuts at: 3531 3531
Size: 5336
SacI G_AGCT'C
Cuts at: 2060 2350 2060
Size: 290 5046
SalI G'TCGA_C
Cuts at: 2373 2373
Size: 5336
SanDI GG'GwC_CC
Cuts at: 1537 1537
Size: 5336
SapI GCTCTTCn'nnn_
Cuts at: 374 3359 3569 374
Size: 2985 210 2141
Fragments arranged by size:
2985 2141 210
SbfI CC_TGCA'GG
Cuts at: 1489 2383 1489
Size: 894 4442
ScaI AGT'ACT
Cuts at: 776 4853 776
Size: 4077 1259
SfcI C'TryA_G
Cuts at: 33 1433 1485 2379 3064 4057 4248 33
Size: 1400 52 894 685 993 191 1121
Fragments arranged by size:
1400 1121 993 894 685 191 52
SfoI GGC'GCC
Cuts at: 2768 3016 2768
Size: 248 5088
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
SmaI CCC'GGG
Cuts at: 2358 2358
Size: 5336
SmlI C'TyrA_G
Cuts at: 1631 2491 3406 3898 4160 1631
Size: 860 915 492 262 2807
Fragments arranged by size:
2807 915 860 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5336
SphI G_CATG'C
Cuts at: 634 2389 2889 634
Size: 1755 500 3081
Fragments arranged by size:
3081 1755 500
SspI AAT'ATT
Cuts at: 975 975
Size: 5336
StyI C'CwwG_G
Cuts at: 107 2470 2824 107
Size: 2363 354 2619
Fragments arranged by size:
2619 2363 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 744 1828 2919 3052 3511 3720 744
Size: 1084 1091 133 459 209 2360
Fragments arranged by size:
2360 1091 1084 459 209 133
TatI w'GTAC_w
Cuts at: 774 908 1047 1327 1344 1594 1627 1832 4851
Size: 134 139 280 17 250 33 205 3019
Cuts at: 4851 774
Size: 1259
Fragments arranged by size:
3019 1259 280 250 205 139 134 33
17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4853 4885 4853
Size: 32 5304
Tth111I GACn'n_nGTC
Cuts at: 3133 3133
Size: 5336
XbaI T'CTAG_A
Cuts at: 2367 2367
Size: 5336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2831 2831
Size: 5336
XhoI C'TCGA_G
Cuts at: 1631 1631
Size: 5336
XmaI C'CCGG_G
Cuts at: 2356 2356
Size: 5336
XmnI GAAnn'nnTTC
Cuts at: 1790 1790
Size: 5336
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BmtI BsgI BsiWI BssHII BstEII BstXI
Bsu36I ClaI FseI FspAI MfeI NheI NruI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI ZraI