Restriction map for
pF1KE9396
(Circular) (Six-base) MAPSORT of: pF1KE9396.seq Check: 63 from: 1 to: 15020
pF1KE9396 15020 bp
With 182 enzymes: SgfI *
May 26, 2016 12:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 11233 11650 11233
Size: 417 14603
AatII G_ACGT'C
Cuts at: 3558 3558
Size: 15020
AccI GT'mk_AC
Cuts at: 1441 5145 6269 6585 8949 9783 10568 11606 12058
Size: 3704 1124 316 2364 834 785 1038 452
Cuts at: 12058 1441
Size: 4403
Fragments arranged by size:
4403 3704 2364 1124 1038 834 785 452
316
Acc65I G'GTAC_C
Cuts at: 2119 9204 10219 12036 2119
Size: 7085 1015 1817 5103
Fragments arranged by size:
7085 5103 1817 1015
AclI AA'CG_TT
Cuts at: 12014 14860 12014
Size: 2846 12174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 590 1511 1649 2114 2276 5059 6947 6971
Size: 198 921 138 465 162 2783 1888 24
Cuts at: 6971 7157 7319 8111 8162 8402 8630 9083 9184
Size: 186 162 792 51 240 228 453 101
Cuts at: 9184 9340 9449 9635 10447 10517 10676 10823 11041
Size: 156 109 186 812 70 159 147 218
Cuts at: 11041 11426 11536 12845 13277 14024 14292 392
Size: 385 110 1309 432 747 268 1120
Fragments arranged by size:
2783 1888 1309 1120 921 812 792 747
465 453 432 385 268 240 228 218
198 186 186 162 162 159 156 147
138 110 109 101 70 51 24
AfeI AGC'GCT
Cuts at: 6298 6298
Size: 15020
AflII C'TTAA_G
Cuts at: 8118 8118
Size: 15020
AflIII A'CryG_T
Cuts at: 207 3315 5397 5805 7985 8403 10188 11435 12372
Size: 3108 2082 408 2180 418 1785 1247 937
Cuts at: 12372 13476 14453 207
Size: 1104 977 774
Fragments arranged by size:
3108 2180 2082 1785 1247 1104 977 937
774 418 408
AgeI A'CCGG_T
Cuts at: 13408 13408
Size: 15020
AhdI GACnn_n'nnGTC
Cuts at: 6996 6996
Size: 15020
AleI CACnn'nnGTG
Cuts at: 4559 4969 5272 11831 4559
Size: 410 303 6559 7748
Fragments arranged by size:
7748 6559 410 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 499 2585 4241 6998 9580 10405 11563 11822 11986
Size: 2086 1656 2757 2582 825 1158 259 164
Cuts at: 11986 499
Size: 3533
Fragments arranged by size:
3533 2757 2582 2086 1656 1158 825 259
164
AlwNI CAG_nnn'CTG
Cuts at: 4593 7792 8808 8824 9331 10096 10275 10870 12325
Size: 3199 1016 16 507 765 179 595 1455
Cuts at: 12325 13892 4593
Size: 1567 5721
Fragments arranged by size:
5721 3199 1567 1455 1016 765 595 507
179 16
ApaI G_GGCC'C
Cuts at: 239 436 10541 239
Size: 197 10105 4718
Fragments arranged by size:
10105 4718 197
ApaLI G'TGCA_C
Cuts at: 7973 13790 7973
Size: 5817 9203
ApoI r'AATT_y
Cuts at: 663 2801 4283 4546 5064 5908 6203 6858 6900
Size: 2138 1482 263 518 844 295 655 42
Cuts at: 6900 7088 8562 11123 11338 12024 14373 14629 663
Size: 188 1474 2561 215 686 2349 256 1054
Fragments arranged by size:
2561 2349 2138 1482 1474 1054 844 686
655 518 295 263 256 215 188 42
AseI AT'TA_AT
Cuts at: 20 1067 5321 20
Size: 1047 4254 9719
Fragments arranged by size:
9719 4254 1047
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 15020
AvaI C'yCGr_G
Cuts at: 145 232 259 435 2080 2671 5128 5984 11852
Size: 87 27 176 1645 591 2457 856 5868
Cuts at: 11852 12040 145
Size: 188 3125
Fragments arranged by size:
5868 3125 2457 1645 856 591 188 176
87 27
AvrII C'CTAG_G
Cuts at: 186 186
Size: 15020
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7286 7319 8279 8312 8529 8562 9714 9747 7286
Size: 33 960 33 217 33 1152 33 12559
Fragments arranged by size:
12559 1152 960 217 33 33 33 33
BamHI G'GATC_C
Cuts at: 3858 4717 9684 12045 3858
Size: 859 4967 2361 6833
Fragments arranged by size:
6833 4967 2361 859
BanI G'GyrC_C
Cuts at: 176 193 264 475 2119 3525 3594 4988 7647
Size: 17 71 211 1644 1406 69 1394 2659
Cuts at: 7647 9204 10002 10219 11314 11986 12036 12450 12698
Size: 1557 798 217 1095 672 50 414 248
Cuts at: 12698 12733 176
Size: 35 2463
Fragments arranged by size:
2659 2463 1644 1557 1406 1394 1095 798
672 414 248 217 211 71 69 50
35 17
BanII G_rGCy'C
Cuts at: 239 436 1257 2191 2205 2408 4088 4469 7009
Size: 197 821 934 14 203 1680 381 2540
Cuts at: 7009 7054 8591 8707 9043 10541 12034 13064 239
Size: 45 1537 116 336 1498 1493 1030 2195
Fragments arranged by size:
2540 2195 1680 1537 1498 1493 1030 934
821 381 336 203 197 116 45 14
BbeI G_GCGC'C
Cuts at: 180 197 268 11990 12454 12702 180
Size: 17 71 11722 464 248 2498
Fragments arranged by size:
11722 2498 464 248 71 17
BbsI GAAGACnn'nnnn_
Cuts at: 371 377 783 1312 1517 2420 2906 3416 5681
Size: 6 406 529 205 903 486 510 2265
Cuts at: 5681 5717 7033 8042 8141 371
Size: 36 1316 1009 99 7250
Fragments arranged by size:
7250 2265 1316 1009 903 529 510 486
406 205 99 36 6
BbvCI CC'TCA_GC
Cuts at: 351 399 2007 4028 4246 10614 351
Size: 48 1608 2021 218 6368 4757
Fragments arranged by size:
6368 4757 2021 1608 218 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 103 137 344 378 103
Size: 34 207 34 14745
Fragments arranged by size:
14745 207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2310 2834 2955 4174 7964 10065 10149 10557 10875
Size: 524 121 1219 3790 2101 84 408 318
Cuts at: 10875 12918 13679 15020 2310
Size: 2043 761 1341 2310
Fragments arranged by size:
3790 2310 2101 2043 1341 1219 761 524
408 318 121 84
BclI T'GATC_A
Cuts at: 5211 5882 6047 6837 9081 12540 5211
Size: 671 165 790 2244 3459 7691
Fragments arranged by size:
7691 3459 2244 790 671 165
BfrBI ATG'CAT
Cuts at: 11799 11799
Size: 15020
BglI GCCn_nnn'nGGC
Cuts at: 191 298 10045 191
Size: 107 9747 5166
Fragments arranged by size:
9747 5166 107
BglII A'GATC_T
Cuts at: 2353 5226 9970 11147 11906 12535 14572 2353
Size: 2873 4744 1177 759 629 2037 2801
Fragments arranged by size:
4744 2873 2801 2037 1177 759 629
BlpI GC'TnA_GC
Cuts at: 1223 1283 3203 8504 8509 9037 10871 11394 12132
Size: 60 1920 5301 5 528 1834 523 738
Cuts at: 12132 1223
Size: 4111
Fragments arranged by size:
5301 4111 1920 1834 738 528 523 60
5
Bme1580I G_kGCm'C
Cuts at: 239 436 1467 1764 7971 7977 9251 10541 12645
Size: 197 1031 297 6207 6 1274 1290 2104
Cuts at: 12645 12738 13794 239
Size: 93 1056 1465
Fragments arranged by size:
6207 2104 1465 1290 1274 1056 1031 297
197 93 6
BmgBI CAC'GTC
Cuts at: 10191 10191
Size: 15020
BmrI ACTGGGnnnn_n'
Cuts at: 1547 1650 6545 6761 8072 8939 9437 10342 10505
Size: 103 4895 216 1311 867 498 905 163
Cuts at: 10505 12232 12313 12648 14792 1547
Size: 1727 81 335 2144 1775
Fragments arranged by size:
4895 2144 1775 1727 1311 905 867 498
335 216 163 103 81
BmtI G_CTAG'C
Cuts at: 9557 10265 10892 9557
Size: 708 627 13685
Fragments arranged by size:
13685 708 627
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 7218 7953 9508 10920 11941 13387 2247
Size: 4971 735 1555 1412 1021 1446 3880
Fragments arranged by size:
4971 3880 1555 1446 1412 1021 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 815 1818 3076 3524 3956 4528 4615 4712
Size: 69 1003 1258 448 432 572 87 97
Cuts at: 4712 5099 5624 6294 6320 6666 7268 10612 10841
Size: 387 525 670 26 346 602 3344 229
Cuts at: 10841 746
Size: 4925
Fragments arranged by size:
4925 3344 1258 1003 670 602 572 525
448 432 387 346 229 97 87 69
26
Bpu10I CC'TnA_GC
Cuts at: 351 399 1881 2007 3140 4028 4246 4365 6198
Size: 48 1482 126 1133 888 218 119 1833
Cuts at: 6198 10614 351
Size: 4416 4757
Fragments arranged by size:
4757 4416 1833 1482 1133 888 218 126
119 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 6144 9745 12196 13075 13567 13865 934
Size: 5210 3601 2451 879 492 298 2089
Fragments arranged by size:
5210 3601 2451 2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 465 605 633 709 1063 2544 2729 3280 4005
Size: 140 28 76 354 1481 185 551 725
Cuts at: 4005 4760 7238 9496 11618 14724 465
Size: 755 2478 2258 2122 3106 761
Fragments arranged by size:
3106 2478 2258 2122 1481 761 755 725
551 354 185 140 76 28
BsaAI yAC'GTr
Cuts at: 12373 12373
Size: 15020
BsaBI GATnn'nnATC
Cuts at: 6 6957 9975 12534 14571 6
Size: 6951 3018 2559 2037 455
Fragments arranged by size:
6951 3018 2559 2037 455
BsaHI Gr'CG_yC
Cuts at: 177 194 265 3555 6687 11987 12451 12699 13401
Size: 17 71 3290 3132 5300 464 248 702
Cuts at: 13401 14897 177
Size: 1496 300
Fragments arranged by size:
5300 3290 3132 1496 702 464 300 248
71 17
BsaWI w'CCGG_w
Cuts at: 1097 4206 6728 9681 10950 12209 12730 13408 13682
Size: 3109 2522 2953 1269 1259 521 678 274
Cuts at: 13682 13829 14240 1097
Size: 147 411 1877
Fragments arranged by size:
3109 2953 2522 1877 1269 1259 678 521
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 585 615 2556 2586 5918 5948 8215 8245 10839
Size: 30 1941 30 3332 30 2267 30 2594
Cuts at: 10839 10869 585
Size: 30 4736
Fragments arranged by size:
4736 3332 2594 2267 1941 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 351 935 1051 1350 1503 1524 1603 1624 1770
Size: 584 116 299 153 21 79 21 146
Cuts at: 1770 2597 2689 3881 4504 5152 5615 6473 6648
Size: 827 92 1192 623 648 463 858 175
Cuts at: 6648 6999 7269 8236 9003 9668 11374 11515 11518
Size: 351 270 967 767 665 1706 141 3
Cuts at: 11518 11930 351
Size: 412 3441
Fragments arranged by size:
3441 1706 1192 967 858 827 767 665
648 623 584 463 412 351 299 270
175 153 146 141 116 92 79 21
21 3
BseYI C'CCAG_C
Cuts at: 322 1766 4004 4886 6713 8389 8932 9027 9887
Size: 1444 2238 882 1827 1676 543 95 860
Cuts at: 9887 10511 11265 12445 13203 13780 14276 322
Size: 624 754 1180 758 577 496 1066
Fragments arranged by size:
2238 1827 1676 1444 1180 1066 882 860
758 754 624 577 543 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1517 5940 7049 11015 11174 11795 1517
Size: 4423 1109 3966 159 621 4742
Fragments arranged by size:
4742 4423 3966 1109 621 159
BsiEI CG_ry'CG
Cuts at: 104 245 293 344 467 3562 6589 12562 12608
Size: 141 48 51 123 3095 3027 5973 46
Cuts at: 12608 13816 104
Size: 1208 1308
Fragments arranged by size:
5973 3095 3027 1308 1208 141 123 51
48 46
BsiHKAI G_wGCw'C
Cuts at: 2205 2408 2574 6280 7009 7054 7977 8591 10394
Size: 203 166 3706 729 45 923 614 1803
Cuts at: 10394 12812 13002 13794 2205
Size: 2418 190 792 3431
Fragments arranged by size:
3706 3431 2418 1803 923 792 729 614
203 190 166 45
BsmI GAATG_Cn'
Cuts at: 1977 4230 7428 1977
Size: 2253 3198 9569
Fragments arranged by size:
9569 3198 2253
BsmBI CGTCTCn'nnnn_
Cuts at: 1790 9281 1790
Size: 7491 7529
Bsp1286I G_dGCh'C
Cuts at: 239 436 1257 1467 1764 2191 2205 2408 2574
Size: 197 821 210 297 427 14 203 166
Cuts at: 2574 4088 4469 6280 7009 7054 7971 7977 8591
Size: 1514 381 1811 729 45 917 6 614
Cuts at: 8591 8707 9043 9251 10394 10541 12034 12645 12738
Size: 116 336 208 1143 147 1493 611 93
Cuts at: 12738 12812 13002 13064 13794 239
Size: 74 190 62 730 1465
Fragments arranged by size:
1811 1514 1493 1465 1143 917 821 730
729 614 611 427 381 336 297 210
208 203 197 190 166 147 116 93
74 62 45 14 6
BspEI T'CCGG_A
Cuts at: 1097 6728 9681 10950 14240 1097
Size: 5631 2953 1269 3290 1877
Fragments arranged by size:
5631 3290 2953 1877 1269
BspHI T'CATG_A
Cuts at: 4758 4776 6624 14196 4758
Size: 18 1848 7572 5582
Fragments arranged by size:
7572 5582 1848 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 447 491 922 3731 3987 10935 11233 11650 12070
Size: 44 431 2809 256 6948 298 417 420
Cuts at: 12070 12284 12586 12967 447
Size: 214 302 381 2500
Fragments arranged by size:
6948 2809 2500 431 420 417 381 302
298 256 214 44
BsrBI CCG'CTC
Cuts at: 50 340 515 1614 4986 11699 13312 13366 14850
Size: 290 175 1099 3372 6713 1613 54 1484
Cuts at: 14850 15015 50
Size: 165 55
Fragments arranged by size:
6713 3372 1613 1484 1099 290 175 165
55 54
BsrDI GCAATG_nn'
Cuts at: 1217 4873 6389 9833 12932 1217
Size: 3656 1516 3444 3099 3305
Fragments arranged by size:
3656 3444 3305 3099 1516
BsrFI r'CCGG_y
Cuts at: 414 488 5096 9999 11683 13018 13199 13408 414
Size: 74 4608 4903 1684 1335 181 209 2026
Fragments arranged by size:
4903 4608 2026 1684 1335 209 181 74
BssSI C'ACGA_G
Cuts at: 632 11829 13291 13649 632
Size: 11197 1462 358 2003
Fragments arranged by size:
11197 2003 1462 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 2017 3337 11750 12397 1219
Size: 798 1320 8413 647 3842
Fragments arranged by size:
8413 3842 1320 798 647
BstBI TT'CG_AA
Cuts at: 6543 6669 13381 6543
Size: 126 6712 8182
Fragments arranged by size:
8182 6712 126
BstEII G'GTnAC_C
Cuts at: 10962 10962
Size: 15020
BstXI CCAn_nnnn'nTGG
Cuts at: 5364 9450 5364
Size: 4086 10934
BstYI r'GATC_y
Cuts at: 1 681 1477 1723 2353 3858 4682 4717 5226
Size: 680 796 246 630 1505 824 35 509
Cuts at: 5226 6571 6952 7060 7363 7627 8020 9051 9684
Size: 1345 381 108 303 264 393 1031 633
Cuts at: 9684 9970 11147 11544 11906 12045 12512 12535 12870
Size: 286 1177 397 362 139 467 23 335
Cuts at: 12870 13116 14128 14214 14226 14572 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1505 1345 1177 1031 1012 824 796 680
633 630 509 467 449 397 393 381
362 346 335 303 286 264 246 246
139 108 86 35 23 12
BstZ17I GTA'TAC
Cuts at: 5146 6270 5146
Size: 1124 13896
Bsu36I CC'TnA_GG
Cuts at: 1607 3599 7925 10842 11416 1607
Size: 1992 4326 2917 574 5211
Fragments arranged by size:
5211 4326 2917 1992 574
BtgI C'CryG_G
Cuts at: 107 199 293 3397 3428 5407 5933 7651 9479
Size: 92 94 3104 31 1979 526 1718 1828
Cuts at: 9479 11622 107
Size: 2143 3505
Fragments arranged by size:
3505 3104 2143 1979 1828 1718 526 94
92 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 12970 13151 13314 12970
Size: 181 163 14676
Fragments arranged by size:
14676 181 163
BtsI GCAGTG_nn'
Cuts at: 1642 1819 1907 3583 3673 3807 5489 7971 9031
Size: 177 88 1676 90 134 1682 2482 1060
Cuts at: 9031 12263 1642
Size: 3232 4399
Fragments arranged by size:
4399 3232 2482 1682 1676 1060 177 134
90 88
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1837 1872 3627 3662 4462 4497 5633 5668 5779
Size: 35 1755 35 800 35 1136 35 111
Cuts at: 5779 5814 1837
Size: 35 11043
Fragments arranged by size:
11043 1755 1136 800 111 35 35 35
35 35
DraI TTT'AAA
Cuts at: 1973 7016 7944 12019 1973
Size: 5043 928 4075 4974
Fragments arranged by size:
5043 4974 4075 928
DraIII CAC_nnn'GTG
Cuts at: 9445 10021 9445
Size: 576 14444
DrdI GACnn_nn'nnGTC
Cuts at: 1782 12726 13584 1782
Size: 10944 858 3218
Fragments arranged by size:
10944 3218 858
EaeI y'GGCC_r
Cuts at: 290 341 464 3345 5787 8424 11625 12605 12779
Size: 51 123 2881 2442 2637 3201 980 174
Cuts at: 12779 13170 13197 14542 290
Size: 391 27 1345 768
Fragments arranged by size:
3201 2881 2637 2442 1345 980 768 391
174 123 51 27
EagI C'GGCC_G
Cuts at: 290 341 464 12605 290
Size: 51 123 12141 2705
Fragments arranged by size:
12141 2705 123 51
EarI CTCTTCn'nnn_
Cuts at: 411 763 2198 2442 2617 3217 3321 6087 6705
Size: 352 1435 244 175 600 104 2766 618
Cuts at: 6705 6900 7176 8847 8974 9392 9429 11012 11492
Size: 195 276 1671 127 418 37 1583 480
Cuts at: 11492 13043 13253 411
Size: 1551 210 2178
Fragments arranged by size:
2766 2178 1671 1583 1551 1435 618 600
480 418 352 276 244 210 195 175
127 104 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 2919 13228 13538 13684 14830 360
Size: 2559 10309 310 146 1146 550
Fragments arranged by size:
10309 2559 1146 550 310 146
EcoICRI GAG'CTC
Cuts at: 2203 2406 7007 7052 8589 2203
Size: 203 4601 45 1537 8634
Fragments arranged by size:
8634 4601 1537 203 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 590 746 815 1511 1649 1818 2114 2276
Size: 198 156 69 696 138 169 296 162
Cuts at: 2276 3076 3524 3956 4528 4615 4712 5059 5099
Size: 800 448 432 572 87 97 347 40
Cuts at: 5099 5624 6294 6320 6666 6947 6971 7157 7268
Size: 525 670 26 346 281 24 186 111
Cuts at: 7268 7319 8111 8162 8402 8630 9083 9184 9340
Size: 51 792 51 240 228 453 101 156
Cuts at: 9340 9449 9635 10447 10517 10612 10676 10823 10841
Size: 109 186 812 70 95 64 147 18
Cuts at: 10841 11041 11426 11536 12845 13277 14024 14292 392
Size: 200 385 110 1309 432 747 268 1120
Fragments arranged by size:
1309 1120 812 800 792 747 696 670
572 525 453 448 432 432 385 347
346 296 281 268 240 228 200 198
186 186 169 162 156 156 147 138
111 110 109 101 97 95 87 70
69 64 51 51 40 26 24 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1919 3058 3190 4040 6599 9650 1919
Size: 1139 132 850 2559 3051 7289
Fragments arranged by size:
7289 3051 2559 1139 850 132
EcoO109I rG'GnC_Cy
Cuts at: 183 236 2667 3301 5170 5599 6531 7197 9475
Size: 53 2431 634 1869 429 932 666 2278
Cuts at: 9475 10537 10538 10702 12159 183
Size: 1062 1 164 1457 3044
Fragments arranged by size:
3044 2431 2278 1869 1457 1062 932 666
634 429 164 53 1
EcoRI G'AATT_C
Cuts at: 5064 5908 6203 6858 7088 12024 5064
Size: 844 295 655 230 4936 8060
Fragments arranged by size:
8060 4936 844 655 295 230
EcoRV GAT'ATC
Cuts at: 6253 6354 6253
Size: 101 14919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 620 902 1248 1517 2047 2375 5394 13039 620
Size: 282 346 269 530 328 3019 7645 2601
Fragments arranged by size:
7645 3019 2601 530 346 328 282 269
FseI GG_CCGG'CC
Cuts at: 418 418
Size: 15020
FspI TGC'GCA
Cuts at: 12801 12801
Size: 15020
HaeII r_GCGC'y
Cuts at: 132 180 197 268 454 4503 6300 11990 12454
Size: 48 17 71 186 4049 1797 5690 464
Cuts at: 12454 12702 13724 14703 132
Size: 248 1022 979 449
Fragments arranged by size:
5690 4049 1797 1022 979 464 449 248
186 71 48 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 719 738 751 788 820 1319 1351 2556
Size: 13 19 13 37 32 499 32 1205
Cuts at: 2556 2588 2665 2697 2994 3026 3216 3248 3430
Size: 32 77 32 297 32 190 32 182
Cuts at: 3430 3433 3462 3465 3672 3704 3748 3780 4921
Size: 3 29 3 207 32 44 32 1141
Cuts at: 4921 4953 5839 5871 6723 6755 6802 6834 6982
Size: 32 886 32 852 32 47 32 148
Cuts at: 6982 7014 7693 7725 7750 7782 8875 8907 8975
Size: 32 679 32 25 32 1093 32 68
Cuts at: 8975 9007 9066 9098 9103 9135 9907 9939 10219
Size: 32 59 32 5 32 772 32 280
Cuts at: 10219 10251 10956 10988 11293 11325 12862 12892 12894
Size: 32 705 32 305 32 1537 30 2
Cuts at: 12894 12924 706
Size: 30 2802
Fragments arranged by size:
2802 1537 1205 1141 1093 886 852 772
705 679 499 305 297 280 207 190
182 148 77 68 59 47 44 37
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 29 25 19 13 13 5 3
3 2
HincII GTy'rAC
Cuts at: 6586 7763 9351 11250 12059 6586
Size: 1177 1588 1899 809 9547
Fragments arranged by size:
9547 1899 1588 1177 809
HindIII A'AGCT_T
Cuts at: 973 3151 9519 973
Size: 2178 6368 6474
Fragments arranged by size:
6474 6368 2178
HpaI GTT'AAC
Cuts at: 9351 11250 9351
Size: 1899 13121
KasI G'GCGC_C
Cuts at: 176 193 264 11986 12450 12698 176
Size: 17 71 11722 464 248 2498
Fragments arranged by size:
11722 2498 464 248 71 17
KpnI G_GTAC'C
Cuts at: 2123 9208 10223 12040 2123
Size: 7085 1015 1817 5103
Fragments arranged by size:
7085 5103 1817 1015
MfeI C'AATT_G
Cuts at: 3452 9415 3452
Size: 5963 9057
MluI A'CGCG_T
Cuts at: 207 14453 207
Size: 14246 774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1688 1894 2085 5258 6245 6427 6586 8237 8579
Size: 206 191 3173 987 182 159 1651 342
Cuts at: 8579 9079 10574 13691 13875 1688
Size: 500 1495 3117 184 2833
Fragments arranged by size:
3173 3117 2833 1651 1495 987 500 342
206 191 184 182 159
MscI TGG'CCA
Cuts at: 5789 8426 12781 5789
Size: 2637 4355 8028
Fragments arranged by size:
8028 4355 2637
MslI CAynn'nnrTG
Cuts at: 1959 4559 4969 5272 5510 6947 8432 9104 10387
Size: 2600 410 303 238 1437 1485 672 1283
Cuts at: 10387 11804 11831 13009 13136 1959
Size: 1417 27 1178 127 3843
Fragments arranged by size:
3843 2600 1485 1437 1417 1283 1178 672
410 303 238 127 27
MspA1I CmG'CkG
Cuts at: 119 201 295 317 533 1287 2125 2971 3334
Size: 82 94 22 216 754 838 846 363
Cuts at: 3334 4518 4590 5830 5898 6448 6717 8089 8808
Size: 1184 72 1240 68 550 269 1372 719
Cuts at: 8808 9295 10045 10372 10618 10677 10740 11307 11514
Size: 487 750 327 246 59 63 567 207
Cuts at: 11514 12131 12445 12805 13818 14063 119
Size: 617 314 360 1013 245 1076
Fragments arranged by size:
1372 1240 1184 1076 1013 846 838 754
750 719 617 567 550 487 363 360
327 314 269 246 245 216 207 94
82 72 68 63 59 22
NaeI GCC'GGC
Cuts at: 416 11685 13201 416
Size: 11269 1516 2235
Fragments arranged by size:
11269 2235 1516
NarI GG'CG_CC
Cuts at: 177 194 265 11987 12451 12699 177
Size: 17 71 11722 464 248 2498
Fragments arranged by size:
11722 2498 464 248 71 17
NcoI C'CATG_G
Cuts at: 107 3397 3428 5407 9479 11622 107
Size: 3290 31 1979 4072 2143 3505
Fragments arranged by size:
4072 3505 3290 2143 1979 31
NdeI CA'TA_TG
Cuts at: 5873 7358 5873
Size: 1485 13535
NgoMIV G'CCGG_C
Cuts at: 414 11683 13199 414
Size: 11269 1516 2235
Fragments arranged by size:
11269 2235 1516
NheI G'CTAG_C
Cuts at: 9553 10261 10888 9553
Size: 708 627 13685
Fragments arranged by size:
13685 708 627
NotI GC'GGCC_GC
Cuts at: 290 341 290
Size: 51 14969
NsiI A_TGCA'T
Cuts at: 11801 11801
Size: 15020
NspI r_CATG'y
Cuts at: 3319 3378 4435 5809 6314 7989 8407 9363 10471
Size: 59 1057 1374 505 1675 418 956 1108
Cuts at: 10471 11439 12073 12573 13480 3319
Size: 968 634 500 907 4859
Fragments arranged by size:
4859 1675 1374 1108 1057 968 956 907
634 505 500 418 59
PasI CC'CwG_GG
Cuts at: 3943 4397 3943
Size: 454 14566
PciI A'CATG_T
Cuts at: 3315 5805 7985 8403 11435 13476 3315
Size: 2490 2180 418 3032 2041 4859
Fragments arranged by size:
4859 3032 2490 2180 2041 418
PflMI CCAn_nnn'nTGG
Cuts at: 4294 5435 7807 8011 9696 9754 10231 10296 10669
Size: 1141 2372 204 1685 58 477 65 373
Cuts at: 10669 4294
Size: 8645
Fragments arranged by size:
8645 2372 1685 1141 477 373 204 65
58
PfoI T'CCnGG_A
Cuts at: 3979 5601 7800 10182 10857 11198 3979
Size: 1622 2199 2382 675 341 7801
Fragments arranged by size:
7801 2382 2199 1622 675 341
PmeI GTTT'AAAC
Cuts at: 12019 12019
Size: 15020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 14159 14191 14159
Size: 32 14988
PpuMI rG'GwC_Cy
Cuts at: 3301 5170 5599 6531 9475 10702 3301
Size: 1869 429 932 2944 1227 7619
Fragments arranged by size:
7619 2944 1869 1227 932 429
PshAI GACnn'nnGTC
Cuts at: 1147 1312 3827 8780 10677 1147
Size: 165 2515 4953 1897 5490
Fragments arranged by size:
5490 4953 2515 1897 165
PsiI TTA'TAA
Cuts at: 2992 6457 2992
Size: 3465 11555
PspOMI G'GGCC_C
Cuts at: 235 432 10537 235
Size: 197 10105 4718
Fragments arranged by size:
10105 4718 197
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6741 6773 6741
Size: 32 14988
PstI C_TGCA'G
Cuts at: 458 1292 1826 2538 3325 3728 4998 5030 6603
Size: 834 534 712 787 403 1270 32 1573
Cuts at: 6603 9609 10932 12067 12752 458
Size: 3006 1323 1135 685 2726
Fragments arranged by size:
3006 2726 1573 1323 1270 1135 834 787
712 685 534 403 32
PvuI CG_AT'CG
Cuts at: 104 3562 104
Size: 3458 11562
PvuII CAG'CTG
Cuts at: 119 1287 2971 3334 4518 4590 5898 6448 8089
Size: 1168 1684 363 1184 72 1308 550 1641
Cuts at: 8089 8808 9295 10618 10740 11307 11514 12445 12805
Size: 719 487 1323 122 567 207 931 360
Cuts at: 12805 119
Size: 2334
Fragments arranged by size:
2334 1684 1641 1323 1308 1184 1168 931
719 567 550 487 363 360 207 122
72
RsrII CG'GwC_CG
Cuts at: 13215 13215
Size: 15020
SacI G_AGCT'C
Cuts at: 2205 2408 7009 7054 8591 2205
Size: 203 4601 45 1537 8634
Fragments arranged by size:
8634 4601 1537 203 45
SacII CC_GC'GG
Cuts at: 202 296 202
Size: 94 14926
SalI G'TCGA_C
Cuts at: 6584 12057 6584
Size: 5473 9547
SanDI GG'GwC_CC
Cuts at: 9475 10702 9475
Size: 1227 13793
SapI GCTCTTCn'nnn_
Cuts at: 3321 13043 13253 3321
Size: 9722 210 5088
Fragments arranged by size:
9722 5088 210
SbfI CC_TGCA'GG
Cuts at: 10932 12067 10932
Size: 1135 13885
ScaI AGT'ACT
Cuts at: 5658 6867 14537 5658
Size: 1209 7670 6141
Fragments arranged by size:
7670 6141 1209
SexAI A'CCwGG_T
Cuts at: 7452 7452
Size: 15020
SfcI C'TryA_G
Cuts at: 33 454 1288 1822 2534 3321 3724 4040 4994
Size: 421 834 534 712 787 403 316 954
Cuts at: 4994 5026 6599 6923 8049 9005 9605 9784 10928
Size: 32 1573 324 1126 956 600 179 1144
Cuts at: 10928 11607 12063 12748 13741 13932 33
Size: 679 456 685 993 191 1121
Fragments arranged by size:
1573 1144 1126 1121 993 956 954 834
787 712 685 679 600 534 456 421
403 324 316 191 179 32
SfoI GGC'GCC
Cuts at: 178 195 266 11988 12452 12700 178
Size: 17 71 11722 464 248 2498
Fragments arranged by size:
11722 2498 464 248 71 17
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 15020
SmaI CCC'GGG
Cuts at: 147 234 261 437 12042 147
Size: 87 27 176 11605 3125
Fragments arranged by size:
11605 3125 176 87 27
SmlI C'TyrA_G
Cuts at: 949 6123 8118 9760 12175 13090 13582 13844 949
Size: 5174 1995 1642 2415 915 492 262 2125
Fragments arranged by size:
5174 2415 2125 1995 1642 915 492 262
SpeI A'CTAG_T
Cuts at: 67 616 2473 2566 6868 8290 67
Size: 549 1857 93 4302 1422 6797
Fragments arranged by size:
6797 4302 1857 1422 549 93
SphI G_CATG'C
Cuts at: 12073 12573 12073
Size: 500 14520
SrfI GCCC'GGGC
Cuts at: 437 437
Size: 15020
SspI AAT'ATT
Cuts at: 1323 2048 2240 11865 1323
Size: 725 192 9625 4478
Fragments arranged by size:
9625 4478 725 192
StuI AGG'CCT
Cuts at: 1023 7580 9656 1023
Size: 6557 2076 6387
Fragments arranged by size:
6557 6387 2076
StyI C'CwwG_G
Cuts at: 107 186 1855 2554 2617 3342 3397 3428 5256
Size: 79 1669 699 63 725 55 31 1828
Cuts at: 5256 5407 7634 8495 9093 9479 10207 11622 12154
Size: 151 2227 861 598 386 728 1415 532
Cuts at: 12154 12508 107
Size: 354 2619
Fragments arranged by size:
2619 2227 1828 1669 1415 861 728 725
699 598 532 386 354 151 79 63
55 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 674 3504 4410 6491 6564 7626 8686 9456 12603
Size: 2830 906 2081 73 1062 1060 770 3147
Cuts at: 12603 12736 13195 13404 674
Size: 133 459 209 2290
Fragments arranged by size:
3147 2830 2290 2081 1062 1060 906 770
459 209 133 73
TatI w'GTAC_w
Cuts at: 3616 4978 5656 6865 7884 8664 14535 3616
Size: 1362 678 1209 1019 780 5871 4101
Fragments arranged by size:
5871 4101 1362 1209 1019 780 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 617 1286 1318 2367 2399 4004 4036 4513
Size: 32 669 32 1049 32 1605 32 477
Cuts at: 4513 4545 4735 4767 5918 5950 8215 8247 8268
Size: 32 190 32 1151 32 2265 32 21
Cuts at: 8268 8300 8904 8936 9580 9612 10762 10794 10839
Size: 32 604 32 644 32 1150 32 45
Cuts at: 10839 10871 14537 14569 585
Size: 32 3666 32 1036
Fragments arranged by size:
3666 2265 1605 1151 1150 1049 1036 669
644 604 477 190 45 32 32 32
32 32 32 32 32 32 32 32
32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 2520 12817 2520
Size: 10297 4723
XbaI T'CTAG_A
Cuts at: 1726 2233 5229 7126 8880 10746 12051 1726
Size: 507 2996 1897 1754 1866 1305 4695
Fragments arranged by size:
4695 2996 1897 1866 1754 1305 507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4736 6172 8691 10915 11048 12515 4736
Size: 1436 2519 2224 133 1467 7241
Fragments arranged by size:
7241 2519 2224 1467 1436 133
XmaI C'CCGG_G
Cuts at: 145 232 259 435 12040 145
Size: 87 27 176 11605 3125
Fragments arranged by size:
11605 3125 176 87 27
XmnI GAAnn'nnTTC
Cuts at: 1698 2286 6062 8039 1698
Size: 588 3776 1977 8679
Fragments arranged by size:
8679 3776 1977 588
ZraI GAC'GTC
Cuts at: 3556 3556
Size: 15020
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BsiWI BsrGI BssHII ClaI FspAI NruI
PacI PmlI PspXI SfiI SgrAI SnaBI SwaI XhoI