Restriction map for
pF1KE9399
(Circular) (Six-base) MAPSORT of: pF1KE9399.seq Check: 8720 from: 1 to: 11258
pF1KE9399 11258 bp
With 182 enzymes: SgfI *
August 18, 2017 10:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4282 4282
Size: 11258
AatII G_ACGT'C
Cuts at: 4055 7825 4055
Size: 3770 7488
AccI GT'mk_AC
Cuts at: 3110 7844 8296 3110
Size: 4734 452 6072
Fragments arranged by size:
6072 4734 452
Acc65I G'GTAC_C
Cuts at: 8274 8274
Size: 11258
AclI AA'CG_TT
Cuts at: 8252 11098 8252
Size: 2846 8412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2104 2150 2660 2789 4621 5062 6038 9083
Size: 414 46 510 129 1832 441 976 3045
Cuts at: 9083 9515 10262 10530 1690
Size: 432 747 268 2418
Fragments arranged by size:
3045 2418 1832 976 747 510 441 432
414 268 129 46
AfeI AGC'GCT
Cuts at: 3916 4471 6258 8233 3916
Size: 555 1787 1975 6941
Fragments arranged by size:
6941 1975 1787 555
AflII C'TTAA_G
Cuts at: 7813 7813
Size: 11258
AflIII A'CryG_T
Cuts at: 2715 3027 3656 4929 7673 8610 9714 10691 2715
Size: 312 629 1273 2744 937 1104 977 3282
Fragments arranged by size:
3282 2744 1273 1104 977 937 629 312
AgeI A'CCGG_T
Cuts at: 9646 9646
Size: 11258
AhdI GACnn_n'nnGTC
Cuts at: 3042 3042
Size: 11258
AleI CACnn'nnGTG
Cuts at: 3691 3869 8069 3691
Size: 178 4200 6880
Fragments arranged by size:
6880 4200 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 813 3004 4186 4549 8060 8224 813
Size: 2191 1182 363 3511 164 3847
Fragments arranged by size:
3847 3511 2191 1182 363 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2467 2499 4963 4995 2467
Size: 32 2464 32 8730
Fragments arranged by size:
8730 2464 32 32
AlwNI CAG_nnn'CTG
Cuts at: 684 934 1188 2319 2328 2352 2902 2929 3460
Size: 250 254 1131 9 24 550 27 531
Cuts at: 3460 4079 4365 5120 5628 8563 10130 684
Size: 619 286 755 508 2935 1567 1812
Fragments arranged by size:
2935 1812 1567 1131 755 619 550 531
508 286 254 250 27 24 9
ApaI G_GGCC'C
Cuts at: 415 708 923 2055 3085 3269 3299 3406 3706
Size: 293 215 1132 1030 184 30 107 300
Cuts at: 3706 3953 4332 4390 6031 6166 6417 6615 7160
Size: 247 379 58 1641 135 251 198 545
Cuts at: 7160 415
Size: 4513
Fragments arranged by size:
4513 1641 1132 1030 545 379 300 293
251 247 215 198 184 135 107 58
30
ApaLI G'TGCA_C
Cuts at: 3610 4089 4498 4850 5521 7662 10028 3610
Size: 479 409 352 671 2141 2366 4840
Fragments arranged by size:
4840 2366 2141 671 479 409 352
ApoI r'AATT_y
Cuts at: 3856 5444 8262 10611 10867 3856
Size: 1588 2818 2349 256 4247
Fragments arranged by size:
4247 2818 2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11258
AvaI C'yCGr_G
Cuts at: 139 168 289 414 554 764 1562 1952 2186
Size: 29 121 125 140 210 798 390 234
Cuts at: 2186 2683 2753 3269 3298 3397 3431 3965 5009
Size: 497 70 516 29 99 34 534 1044
Cuts at: 5009 5108 6165 6341 7442 7637 8090 8278 139
Size: 99 1057 176 1101 195 453 188 3119
Fragments arranged by size:
3119 1101 1057 1044 798 534 516 497
453 390 234 210 195 188 176 140
125 121 99 99 70 34 29 29
AvrII C'CTAG_G
Cuts at: 6468 6468
Size: 11258
BamHI G'GATC_C
Cuts at: 3889 4782 4809 8283 3889
Size: 893 27 3474 6864
Fragments arranged by size:
6864 3474 893 27
BanI G'GyrC_C
Cuts at: 184 711 1579 2763 3524 3983 5050 5438 5928
Size: 527 868 1184 761 459 1067 388 490
Cuts at: 5928 6522 7253 8224 8274 8688 8936 8971 184
Size: 594 731 971 50 414 248 35 2471
Fragments arranged by size:
2471 1184 1067 971 868 761 731 594
527 490 459 414 388 248 50 35
BanII G_rGCy'C
Cuts at: 290 326 415 708 746 863 923 1010 1162
Size: 36 89 293 38 117 60 87 152
Cuts at: 1162 2055 2127 2280 2572 2921 3085 3269 3299
Size: 893 72 153 292 349 164 184 30
Cuts at: 3299 3398 3406 3706 3953 4220 4332 4375 4390
Size: 99 8 300 247 267 112 43 15
Cuts at: 4390 4595 5036 5549 5601 5697 6031 6113 6166
Size: 205 441 513 52 96 334 82 53
Cuts at: 6166 6273 6417 6615 6656 7136 7160 7282 7554
Size: 107 144 198 41 480 24 122 272
Cuts at: 7554 8078 8165 8272 9302 290
Size: 524 87 107 1030 2246
Fragments arranged by size:
2246 1030 893 524 513 480 441 349
334 300 293 292 272 267 247 205
198 184 164 153 152 144 122 117
112 107 107 99 96 89 87 87
82 72 60 53 52 43 41 38
36 30 24 15 8
BbeI G_GCGC'C
Cuts at: 188 3528 3987 5054 6526 8228 8692 8940 188
Size: 3340 459 1067 1472 1702 464 248 2506
Fragments arranged by size:
3340 2506 1702 1472 1067 464 459 248
BbsI GAAGACnn'nnnn_
Cuts at: 1637 1760 3187 4151 4235 4953 5139 6026 7825
Size: 123 1427 964 84 718 186 887 1799
Cuts at: 7825 1637
Size: 5070
Fragments arranged by size:
5070 1799 1427 964 887 718 186 123
84
BbvCI CC'TCA_GC
Cuts at: 1268 2212 4788 5083 5918 6323 1268
Size: 944 2576 295 835 405 6203
Fragments arranged by size:
6203 2576 944 835 405 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6322 6356 6322
Size: 34 11224
BciVI GTATCCnnnnn_n'
Cuts at: 2823 7026 9156 9917 11258 2823
Size: 4203 2130 761 1341 2823
Fragments arranged by size:
4203 2823 2130 1341 761
BclI T'GATC_A
Cuts at: 1019 8778 1019
Size: 7759 3499
BfrBI ATG'CAT
Cuts at: 3928 8037 3928
Size: 4109 7149
BglI GCCn_nnn'nGGC
Cuts at: 112 971 980 4308 5466 6139 6161 112
Size: 859 9 3328 1158 673 22 5209
Fragments arranged by size:
5209 3328 1158 859 673 22 9
BglII A'GATC_T
Cuts at: 2087 2612 8773 10810 2087
Size: 525 6161 2037 2535
Fragments arranged by size:
6161 2535 2037 525
BlpI GC'TnA_GC
Cuts at: 2174 2312 2566 4369 6898 8370 2174
Size: 138 254 1803 2529 1472 5062
Fragments arranged by size:
5062 2529 1803 1472 254 138
Bme1580I G_kGCm'C
Cuts at: 415 708 923 1582 1710 1866 2055 2793 3085
Size: 293 215 659 128 156 189 738 292
Cuts at: 3085 3269 3299 3406 3614 3706 3953 4093 4332
Size: 184 30 107 208 92 247 140 239
Cuts at: 4332 4390 4502 4854 5109 5525 5711 5933 6031
Size: 58 112 352 255 416 186 222 98
Cuts at: 6031 6166 6355 6417 6615 7160 7666 8883 8976
Size: 135 189 62 198 545 506 1217 93
Cuts at: 8976 10032 415
Size: 1056 1641
Fragments arranged by size:
1641 1217 1056 738 659 545 506 416
352 293 292 255 247 239 222 215
208 198 189 189 186 184 156 140
135 128 112 107 98 93 92 62
58 30
BmgBI CAC'GTC
Cuts at: 3867 4932 7768 3867
Size: 1065 2836 7357
Fragments arranged by size:
7357 2836 1065
BmrI ACTGGGnnnn_n'
Cuts at: 503 1405 1879 4056 4203 4401 6742 7242 8470
Size: 902 474 2177 147 198 2341 500 1228
Cuts at: 8470 8551 8886 11030 503
Size: 81 335 2144 731
Fragments arranged by size:
2341 2177 2144 1228 902 731 500 474
335 198 147 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1599 2691 3327 4236 5628 9625 1599
Size: 1092 636 909 1392 3997 3232
Fragments arranged by size:
3997 3232 1392 1092 909 636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1488 2153 2264 2594 2951 3218 3817 3925
Size: 172 665 111 330 357 267 599 108
Cuts at: 3925 4019 4085 4318 4342 4595 4760 4852 5421
Size: 94 66 233 24 253 165 92 569
Cuts at: 5421 5470 5512 5615 5654 6287 7147 7801 1316
Size: 49 42 103 39 633 860 654 4773
Fragments arranged by size:
4773 860 665 654 633 599 569 357
330 267 253 233 172 165 111 108
103 94 92 66 49 42 39 24
Bpu10I CC'TnA_GC
Cuts at: 857 1268 2138 2180 2212 4321 4788 5083 5603
Size: 411 870 42 32 2109 467 295 520
Cuts at: 5603 5918 5923 6323 6650 7753 857
Size: 315 5 400 327 1103 4362
Fragments arranged by size:
4362 2109 1103 870 520 467 411 400
327 315 295 42 32 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2479 3496 4969 7261 8434 9313 9805 10103 2479
Size: 1017 1473 2292 1173 879 492 298 3634
Fragments arranged by size:
3634 2292 1473 1173 1017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 767 1788 4549 4747 5807 10962 767
Size: 1021 2761 198 1060 5155 1063
Fragments arranged by size:
5155 2761 1063 1060 1021 198
BsaAI yAC'GTr
Cuts at: 1499 1861 3028 8103 8611 1499
Size: 362 1167 5075 508 4146
Fragments arranged by size:
5075 4146 1167 508 362
BsaBI GATnn'nnATC
Cuts at: 6 7909 8772 10809 6
Size: 7903 863 2037 455
Fragments arranged by size:
7903 2037 863 455
BsaHI Gr'CG_yC
Cuts at: 185 2842 3420 3525 3984 4052 4634 5051 6523
Size: 2657 578 105 459 68 582 417 1472
Cuts at: 6523 7822 8027 8225 8689 8937 9639 11135 185
Size: 1299 205 198 464 248 702 1496 308
Fragments arranged by size:
2657 1496 1472 1299 702 582 578 464
459 417 308 248 205 198 105 68
BsaWI w'CCGG_w
Cuts at: 1626 2633 3113 7191 7655 8447 8968 9646 9920
Size: 1007 480 4078 464 792 521 678 274
Cuts at: 9920 10067 10478 1626
Size: 147 411 2406
Fragments arranged by size:
4078 2406 1007 792 678 521 480 464
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1362 1392 1572 1602 2097 2127 2467 2497 4963
Size: 30 180 30 495 30 340 30 2466
Cuts at: 4963 4993 5371 5401 6040 6070 7393 7423 1362
Size: 30 378 30 639 30 1323 30 5197
Fragments arranged by size:
5197 2466 1323 639 495 378 340 180
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 454 481 484 584 587 1285 1319 1337
Size: 3 27 3 100 3 698 34 18
Cuts at: 1337 1367 1469 1474 1577 1704 1862 1958 2016
Size: 30 102 5 103 127 158 96 58
Cuts at: 2016 2099 2102 2105 2141 2482 2488 2602 3289
Size: 83 3 3 36 341 6 114 687
Cuts at: 3289 3316 3404 3478 3814 4561 4981 5518 5657
Size: 27 88 74 336 747 420 537 139
Cuts at: 5657 5891 6196 6199 6520 6814 7621 8173 451
Size: 234 305 3 321 294 807 552 3536
Fragments arranged by size:
3536 807 747 698 687 552 537 420
341 336 321 305 294 234 158 139
127 114 103 102 100 96 88 83
74 58 36 34 30 27 27 18
6 5 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 318 407 536 1491 1534 1603 1974 2345 3259
Size: 89 129 955 43 69 371 371 914
Cuts at: 3259 3821 3952 4094 4288 5826 5854 6099 6136
Size: 562 131 142 194 1538 28 245 37
Cuts at: 6136 6253 6573 6696 6721 7138 7553 7694 8683
Size: 117 320 123 25 417 415 141 989
Cuts at: 8683 9441 10018 10514 318
Size: 758 577 496 1062
Fragments arranged by size:
1538 1062 989 955 914 758 577 562
496 417 415 371 371 320 245 194
142 141 131 129 123 117 89 69
43 37 28 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 2530 3052 4386 4417 6352 7768 697
Size: 1833 522 1334 31 1935 1416 4187
Fragments arranged by size:
4187 1935 1833 1416 1334 522 31
BsiEI CG_ry'CG
Cuts at: 104 178 996 6966 8800 8846 10054 104
Size: 74 818 5970 1834 46 1208 1308
Fragments arranged by size:
5970 1834 1308 1208 818 74 46
BsiHKAI G_wGCw'C
Cuts at: 1504 3614 3686 4093 4358 4375 4502 4854 5036
Size: 2110 72 407 265 17 127 352 182
Cuts at: 5036 5300 5389 5407 5525 5601 7282 7666 7709
Size: 264 89 18 118 76 1681 384 43
Cuts at: 7709 8165 8272 9050 9240 10032 1504
Size: 456 107 778 190 792 2730
Fragments arranged by size:
2730 2110 1681 792 778 456 407 384
352 265 264 190 182 127 118 107
89 76 72 43 18 17
BsmI GAATG_Cn'
Cuts at: 4795 4795
Size: 11258
BsmBI CGTCTCn'nnnn_
Cuts at: 1899 1899
Size: 11258
Bsp1286I G_dGCh'C
Cuts at: 290 326 415 708 746 863 923 1010 1162
Size: 36 89 293 38 117 60 87 152
Cuts at: 1162 1504 1582 1710 1866 2055 2127 2280 2572
Size: 342 78 128 156 189 72 153 292
Cuts at: 2572 2793 2921 3085 3269 3299 3398 3406 3614
Size: 221 128 164 184 30 99 8 208
Cuts at: 3614 3686 3706 3953 4093 4220 4332 4358 4375
Size: 72 20 247 140 127 112 26 17
Cuts at: 4375 4390 4502 4595 4854 5036 5109 5300 5389
Size: 15 112 93 259 182 73 191 89
Cuts at: 5389 5407 5525 5549 5601 5697 5711 5933 6031
Size: 18 118 24 52 96 14 222 98
Cuts at: 6031 6113 6166 6273 6355 6417 6615 6656 7136
Size: 82 53 107 82 62 198 41 480
Cuts at: 7136 7160 7282 7554 7666 7709 8078 8165 8272
Size: 24 122 272 112 43 369 87 107
Cuts at: 8272 8883 8976 9050 9240 9302 10032 290
Size: 611 93 74 190 62 730 1516
Fragments arranged by size:
1516 730 611 480 369 342 293 292
272 259 247 222 221 208 198 191
190 189 184 182 164 156 153 152
140 128 128 127 122 118 117 112
112 112 107 107 99 98 96 93
93 89 89 87 87 82 82 78
74 73 72 72 62 62 60 53
52 43 41 38 36 30 26 24
24 20 18 17 15 14 8
BspEI T'CCGG_A
Cuts at: 1626 7191 7655 10478 1626
Size: 5565 464 2823 2406
Fragments arranged by size:
5565 2823 2406 464
BspHI T'CATG_A
Cuts at: 10434 10434
Size: 11258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4282 6440 7241 8308 8522 8824 9205 4282
Size: 2158 801 1067 214 302 381 6335
Fragments arranged by size:
6335 2158 1067 801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1551 1712 2670 3810 4325 7937 9550 9604
Size: 1501 161 958 1140 515 3612 1613 54
Cuts at: 9604 11088 11253 50
Size: 1484 165 55
Fragments arranged by size:
3612 1613 1501 1484 1140 958 515 165
161 55 54
BsrDI GCAATG_nn'
Cuts at: 8049 9170 8049
Size: 1121 10137
BsrFI r'CCGG_y
Cuts at: 173 187 341 361 1517 3473 6965 9256 9437
Size: 14 154 20 1156 1956 3492 2291 181
Cuts at: 9437 9646 173
Size: 209 1785
Fragments arranged by size:
3492 2291 1956 1785 1156 209 181 154
20 14
BsrGI T'GTAC_A
Cuts at: 4129 4268 4904 4129
Size: 139 636 10483
Fragments arranged by size:
10483 636 139
BssSI C'ACGA_G
Cuts at: 3745 9529 9887 3745
Size: 5784 358 5116
Fragments arranged by size:
5784 5116 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2328 2337 3860 7704 7988 8635 2328
Size: 9 1523 3844 284 647 4951
Fragments arranged by size:
4951 3844 1523 647 284 9
BstBI TT'CG_AA
Cuts at: 9619 9619
Size: 11258
BstEII G'GTnAC_C
Cuts at: 2238 2238
Size: 11258
BstXI CCAn_nnnn'nTGG
Cuts at: 3871 4383 5862 5888 6099 7779 3871
Size: 512 1479 26 211 1680 7350
Fragments arranged by size:
7350 1680 1479 512 211 26
BstYI r'GATC_y
Cuts at: 1 1141 2087 2612 3889 3992 4238 4782 4809
Size: 1140 946 525 1277 103 246 544 27
Cuts at: 4809 8283 8750 8773 9108 9354 10366 10452 10464
Size: 3474 467 23 335 246 1012 86 12
Cuts at: 10464 10810 1
Size: 346 449
Fragments arranged by size:
3474 1277 1140 1012 946 544 525 467
449 346 335 246 246 103 86 27
23 12
BstZ17I GTA'TAC
Cuts at: 3111 3111
Size: 11258
Bsu36I CC'TnA_GG
Cuts at: 1918 2676 3215 3461 5970 6425 7040 7712 1918
Size: 758 539 246 2509 455 615 672 5464
Fragments arranged by size:
5464 2509 758 672 615 539 455 246
BtgI C'CryG_G
Cuts at: 107 178 204 367 966 975 990 1613 1643
Size: 71 26 163 599 9 15 623 30
Cuts at: 1643 2759 5054 5461 6143 6156 6785 6839 7626
Size: 1116 2295 407 682 13 629 54 787
Cuts at: 7626 7790 7860 107
Size: 164 70 3505
Fragments arranged by size:
3505 2295 1116 787 682 629 623 599
407 164 163 71 70 54 30 26
15 13 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 9208 9389 9552 9208
Size: 181 163 10914
Fragments arranged by size:
10914 181 163
BtsI GCAGTG_nn'
Cuts at: 453 2198 2962 3106 3448 4180 4390 4738 5638
Size: 1745 764 144 342 732 210 348 900
Cuts at: 5638 6909 7065 7371 8501 453
Size: 1271 156 306 1130 3210
Fragments arranged by size:
3210 1745 1271 1130 900 764 732 348
342 306 210 156 144
ClaI AT'CG_AT
Cuts at: 2525 2525
Size: 11258
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3867 3902 3867
Size: 35 11223
DraI TTT'AAA
Cuts at: 8257 8257
Size: 11258
DraIII CAC_nnn'GTG
Cuts at: 1861 3839 4197 4409 7704 1861
Size: 1978 358 212 3295 5415
Fragments arranged by size:
5415 3295 1978 358 212
DrdI GACnn_nn'nnGTC
Cuts at: 3881 5143 5846 6231 8964 9822 3881
Size: 1262 703 385 2733 858 5317
Fragments arranged by size:
5317 2733 1262 858 703 385
EaeI y'GGCC_r
Cuts at: 175 784 993 2594 4482 5458 5644 6089 6963
Size: 609 209 1601 1888 976 186 445 874
Cuts at: 6963 7532 7793 8843 9017 9408 9435 10780 175
Size: 569 261 1050 174 391 27 1345 653
Fragments arranged by size:
1888 1601 1345 1050 976 874 653 609
569 445 391 261 209 186 174 27
EagI C'GGCC_G
Cuts at: 175 993 6963 8843 175
Size: 818 5970 1880 2590
Fragments arranged by size:
5970 2590 1880 818
EarI CTCTTCn'nnn_
Cuts at: 1128 1199 1238 1292 1504 1897 5371 5471 6116
Size: 71 39 54 212 393 3474 100 645
Cuts at: 6116 7283 7730 9281 9491 1128
Size: 1167 447 1551 210 2895
Fragments arranged by size:
3474 2895 1551 1167 645 447 393 212
210 100 71 54 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 224 293 316 337 1561 4969 5206 6225 6844
Size: 69 23 21 1224 3408 237 1019 619
Cuts at: 6844 9466 9776 9922 11068 224
Size: 2622 310 146 1146 414
Fragments arranged by size:
3408 2622 1224 1146 1019 619 414 310
237 146 69 23 21
EcoICRI GAG'CTC
Cuts at: 4373 5034 5599 7280 8163 8270 4373
Size: 661 565 1681 883 107 7361
Fragments arranged by size:
7361 1681 883 661 565 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1488 1690 2104 2150 2153 2264 2594 2660
Size: 172 202 414 46 3 111 330 66
Cuts at: 2660 2789 2951 3218 3817 3925 4019 4085 4318
Size: 129 162 267 599 108 94 66 233
Cuts at: 4318 4342 4595 4621 4760 4852 5062 5421 5470
Size: 24 253 26 139 92 210 359 49
Cuts at: 5470 5512 5615 5654 6038 6287 7147 7801 9083
Size: 42 103 39 384 249 860 654 1282
Cuts at: 9083 9515 10262 10530 1316
Size: 432 747 268 2044
Fragments arranged by size:
2044 1282 860 747 654 599 432 414
384 359 330 268 267 253 249 233
210 202 172 162 139 129 111 108
103 94 92 66 66 49 46 42
39 26 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 801 2031 2356 2696 2746 3532 3799 4348 5969
Size: 1230 325 340 50 786 267 549 1621
Cuts at: 5969 801
Size: 6090
Fragments arranged by size:
6090 1621 1230 786 549 340 325 267
50
EcoO109I rG'GnC_Cy
Cuts at: 333 411 793 919 931 938 1621 2036 2052
Size: 78 382 126 12 7 683 415 16
Cuts at: 2052 2626 2680 2701 2814 2899 2936 3044 3081
Size: 574 54 21 113 85 37 108 37
Cuts at: 3081 3082 3265 3266 3295 3402 3403 3672 3702
Size: 1 183 1 29 107 1 269 30
Cuts at: 3702 3703 3949 4317 4328 4329 4341 4362 4441
Size: 1 246 368 11 1 12 21 79
Cuts at: 4441 4836 5562 5732 6162 6390 6413 6429 6475
Size: 395 726 170 430 228 23 16 46
Cuts at: 6475 6611 6612 6817 7107 7157 7212 7323 8397
Size: 136 1 205 290 50 55 111 1074
Cuts at: 8397 333
Size: 3194
Fragments arranged by size:
3194 1074 726 683 574 430 415 395
382 368 290 269 246 228 205 183
170 136 126 113 111 108 107 85
79 78 55 54 50 46 37 37
30 29 23 21 21 16 16 12
12 11 7 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 5444 8262 5444
Size: 2818 8440
EcoRV GAT'ATC
Cuts at: 3369 3369
Size: 11258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5163 5798 7683 9277 5163
Size: 635 1885 1594 7144
Fragments arranged by size:
7144 1885 1594 635
FseI GG_CCGG'CC
Cuts at: 177 365 1521 177
Size: 188 1156 9914
Fragments arranged by size:
9914 1156 188
FspI TGC'GCA
Cuts at: 2992 4179 4730 9039 2992
Size: 1187 551 4309 5211
Fragments arranged by size:
5211 4309 1187 551
FspAI rTGC'GCAy
Cuts at: 2992 4179 2992
Size: 1187 10071
HaeII r_GCGC'y
Cuts at: 188 553 2961 3342 3528 3918 3987 4473 5054
Size: 365 2408 381 186 390 69 486 581
Cuts at: 5054 5427 5610 5663 6260 6526 8228 8235 8692
Size: 373 183 53 597 266 1702 7 457
Cuts at: 8692 8940 9962 10941 188
Size: 248 1022 979 505
Fragments arranged by size:
2408 1702 1022 979 597 581 505 486
457 390 381 373 365 266 248 186
183 69 53 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 1362 1394 1898 1930 2295 2327 2615
Size: 32 888 32 504 32 365 32 288
Cuts at: 2615 2647 2818 2821 2850 2853 3028 3060 4864
Size: 32 171 3 29 3 175 32 1804
Cuts at: 4864 4896 6040 6072 6172 6204 6421 6453 7282
Size: 32 1144 32 100 32 217 32 829
Cuts at: 7282 7314 7841 7873 9100 9130 9132 9162 442
Size: 32 527 32 1227 30 2 30 2538
Fragments arranged by size:
2538 1804 1227 1144 888 829 527 504
365 288 217 175 171 100 32 32
32 32 32 32 32 32 32 32
32 32 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1099 3054 4935 6690 7760 7946 8297 1099
Size: 1955 1881 1755 1070 186 351 4060
Fragments arranged by size:
4060 1955 1881 1755 1070 351 186
KasI G'GCGC_C
Cuts at: 184 3524 3983 5050 6522 8224 8688 8936 184
Size: 3340 459 1067 1472 1702 464 248 2506
Fragments arranged by size:
3340 2506 1702 1472 1067 464 459 248
KpnI G_GTAC'C
Cuts at: 8278 8278
Size: 11258
MfeI C'AATT_G
Cuts at: 1060 1060
Size: 11258
MluI A'CGCG_T
Cuts at: 10691 10691
Size: 11258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 1119 1621 2642 3403 3985 6825 9929 10113
Size: 634 502 1021 761 582 2840 3104 184
Cuts at: 10113 485
Size: 1630
Fragments arranged by size:
3104 2840 1630 1021 761 634 582 502
184
MscI TGG'CCA
Cuts at: 4484 7795 9019 4484
Size: 3311 1224 6723
Fragments arranged by size:
6723 3311 1224
MslI CAynn'nnrTG
Cuts at: 491 3691 3869 4531 4724 5293 5886 6049 7514
Size: 3200 178 662 193 569 593 163 1465
Cuts at: 7514 7795 8042 8069 8101 9247 9374 491
Size: 281 247 27 32 1146 127 2375
Fragments arranged by size:
3200 2375 1465 1146 662 593 569 281
247 193 178 163 127 32 27
MspA1I CmG'CkG
Cuts at: 180 206 369 378 838 902 992 1285 1542
Size: 26 163 9 460 64 90 293 257
Cuts at: 1542 2173 2385 2460 2565 4307 6136 6288 6577
Size: 631 212 75 105 1742 1829 152 289
Cuts at: 6577 7138 8369 8683 9043 10056 10301 180
Size: 561 1231 314 360 1013 245 1137
Fragments arranged by size:
1829 1742 1231 1137 1013 631 561 460
360 314 293 289 257 245 212 163
152 105 90 75 64 26 9
NaeI GCC'GGC
Cuts at: 175 189 343 363 1519 3475 9439 175
Size: 14 154 20 1156 1956 5964 1994
Fragments arranged by size:
5964 1994 1956 1156 154 20 14
NarI GG'CG_CC
Cuts at: 185 3525 3984 5051 6523 8225 8689 8937 185
Size: 3340 459 1067 1472 1702 464 248 2506
Fragments arranged by size:
3340 2506 1702 1472 1067 464 459 248
NcoI C'CATG_G
Cuts at: 107 2759 5461 6143 7626 7790 107
Size: 2652 2702 682 1483 164 3575
Fragments arranged by size:
3575 2702 2652 1483 682 164
NgoMIV G'CCGG_C
Cuts at: 173 187 341 361 1517 3473 9437 173
Size: 14 154 20 1156 1956 5964 1994
Fragments arranged by size:
5964 1994 1956 1156 154 20 14
NsiI A_TGCA'T
Cuts at: 3930 8039 3930
Size: 4109 7149
NspI r_CATG'y
Cuts at: 740 2719 4204 4999 7453 7677 8311 8811 9718
Size: 1979 1485 795 2454 224 634 500 907
Cuts at: 9718 740
Size: 2280
Fragments arranged by size:
2454 2280 1979 1485 907 795 634 500
224
PasI CC'CwG_GG
Cuts at: 2742 2902 3194 3292 5325 6607 6616 2742
Size: 160 292 98 2033 1282 9 7384
Fragments arranged by size:
7384 2033 1282 292 160 98 9
PciI A'CATG_T
Cuts at: 2715 7673 9714 2715
Size: 4958 2041 4259
Fragments arranged by size:
4958 4259 2041
PflMI CCAn_nnn'nTGG
Cuts at: 934 3578 3750 4652 6800 7054 934
Size: 2644 172 902 2148 254 5138
Fragments arranged by size:
5138 2644 2148 902 254 172
PfoI T'CCnGG_A
Cuts at: 927 1504 2029 2801 3902 5129 5171 5725 6116
Size: 577 525 772 1101 1227 42 554 391
Cuts at: 6116 6799 7163 927
Size: 683 364 5022
Fragments arranged by size:
5022 1227 1101 772 683 577 554 525
391 364 42
PmeI GTTT'AAAC
Cuts at: 8257 8257
Size: 11258
PmlI CAC'GTG
Cuts at: 1861 8103 1861
Size: 6242 5016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2499 3739 3771 4963 4995 7360 7392 10397
Size: 32 1240 32 1192 32 2365 32 3005
Cuts at: 10397 10429 2467
Size: 32 3296
Fragments arranged by size:
3296 3005 2365 1240 1192 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 931 938 2680 2701 2814 2936 3044 4441 4836
Size: 7 1742 21 113 122 108 1397 395
Cuts at: 4836 5562 6390 6429 6475 7107 7212 931
Size: 726 828 39 46 632 105 4977
Fragments arranged by size:
4977 1742 1397 828 726 632 395 122
113 108 105 46 39 21 7
PshAI GACnn'nnGTC
Cuts at: 1062 4841 4930 1062
Size: 3779 89 7390
Fragments arranged by size:
7390 3779 89
PspOMI G'GGCC_C
Cuts at: 411 704 919 2051 3081 3265 3295 3402 3702
Size: 293 215 1132 1030 184 30 107 300
Cuts at: 3702 3949 4328 4386 6027 6162 6413 6611 7156
Size: 247 379 58 1641 135 251 198 545
Cuts at: 7156 411
Size: 4513
Fragments arranged by size:
4513 1641 1132 1030 545 379 300 293
251 247 215 198 184 135 107 58
30
PstI C_TGCA'G
Cuts at: 802 2324 2333 2390 3323 3497 5502 6405 8305
Size: 1522 9 57 933 174 2005 903 1900
Cuts at: 8305 8990 802
Size: 685 3070
Fragments arranged by size:
3070 2005 1900 1522 933 903 685 174
57 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11258
PvuII CAG'CTG
Cuts at: 838 902 1285 2173 2385 6288 6577 8683 9043
Size: 64 383 888 212 3903 289 2106 360
Cuts at: 9043 838
Size: 3053
Fragments arranged by size:
3903 3053 2106 888 383 360 289 212
64
RsrII CG'GwC_CG
Cuts at: 4279 4563 9453 4279
Size: 284 4890 6084
Fragments arranged by size:
6084 4890 284
SacI G_AGCT'C
Cuts at: 4375 5036 5601 7282 8165 8272 4375
Size: 661 565 1681 883 107 7361
Fragments arranged by size:
7361 1681 883 661 565 107
SacII CC_GC'GG
Cuts at: 181 207 370 993 181
Size: 26 163 623 10446
Fragments arranged by size:
10446 623 163 26
SalI G'TCGA_C
Cuts at: 8295 8295
Size: 11258
SanDI GG'GwC_CC
Cuts at: 938 2936 938
Size: 1998 9260
SapI GCTCTTCn'nnn_
Cuts at: 5371 5471 7283 9281 9491 5371
Size: 100 1812 1998 210 7138
Fragments arranged by size:
7138 1998 1812 210 100
SbfI CC_TGCA'GG
Cuts at: 802 2333 8305 802
Size: 1531 5972 3755
Fragments arranged by size:
5972 3755 1531
ScaI AGT'ACT
Cuts at: 4651 7918 10775 4651
Size: 3267 2857 5134
Fragments arranged by size:
5134 3267 2857
SexAI A'CCwGG_T
Cuts at: 3835 3835
Size: 11258
SfcI C'TryA_G
Cuts at: 33 798 1262 2320 2329 2386 3319 3493 5498
Size: 765 464 1058 9 57 933 174 2005
Cuts at: 5498 6401 7845 8301 8986 9979 10170 33
Size: 903 1444 456 685 993 191 1121
Fragments arranged by size:
2005 1444 1121 1058 993 933 903 765
685 464 456 191 174 57 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5466 5466
Size: 11258
SfoI GGC'GCC
Cuts at: 186 3526 3985 5052 6524 8226 8690 8938 186
Size: 3340 459 1067 1472 1702 464 248 2506
Fragments arranged by size:
3340 2506 1702 1472 1067 464 459 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11258
SgrAI Cr'CCGG_yG
Cuts at: 187 187
Size: 11258
SmaI CCC'GGG
Cuts at: 141 170 291 1954 2755 3271 3399 3967 5110
Size: 29 121 1663 801 516 128 568 1143
Cuts at: 5110 6167 8280 141
Size: 1057 2113 3119
Fragments arranged by size:
3119 2113 1663 1143 1057 801 568 516
128 121 29
SmlI C'TyrA_G
Cuts at: 2494 3511 4984 7240 7813 8413 9328 9820 10082
Size: 1017 1473 2256 573 600 915 492 262
Cuts at: 10082 2494
Size: 3670
Fragments arranged by size:
3670 2256 1473 1017 915 600 573 492
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11258
SphI G_CATG'C
Cuts at: 4204 4999 7453 8311 8811 4204
Size: 795 2454 858 500 6651
Fragments arranged by size:
6651 2454 858 795 500
SrfI GCCC'GGGC
Cuts at: 2755 5110 2755
Size: 2355 8903
StuI AGG'CCT
Cuts at: 2335 3220 6560 7024 7871 2335
Size: 885 3340 464 847 5722
Fragments arranged by size:
5722 3340 885 847 464
StyI C'CwwG_G
Cuts at: 107 1067 2759 4376 4883 5461 5735 6143 6393
Size: 960 1692 1617 507 578 274 408 250
Cuts at: 6393 6468 6555 6636 7626 7790 8392 8746 107
Size: 75 87 81 990 164 602 354 2619
Fragments arranged by size:
2619 1692 1617 990 960 602 578 507
408 354 274 250 164 87 81 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3305 3800 4108 4185 4420 4451 7828 8841 8974
Size: 495 308 77 235 31 3377 1013 133
Cuts at: 8974 9433 9642 3305
Size: 459 209 4921
Fragments arranged by size:
4921 3377 1013 495 459 308 235 209
133 77 31
TatI w'GTAC_w
Cuts at: 4129 4268 4649 4904 5396 7916 10773 4129
Size: 139 381 255 492 2520 2857 4614
Fragments arranged by size:
4614 2857 2520 492 381 255 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 636 668 1572 1604 4042 4074 7393 7425 10775
Size: 32 904 32 2438 32 3319 32 3350
Cuts at: 10775 10807 636
Size: 32 1087
Fragments arranged by size:
3350 3319 2438 1087 904 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 392 3011 9055 392
Size: 2619 6044 2595
Fragments arranged by size:
6044 2619 2595
XbaI T'CTAG_A
Cuts at: 8289 8289
Size: 11258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1135 3900 4043 5625 7216 7833 8753 1135
Size: 2765 143 1582 1591 617 920 3640
Fragments arranged by size:
3640 2765 1591 1582 920 617 143
XmaI C'CCGG_G
Cuts at: 139 168 289 1952 2753 3269 3397 3965 5108
Size: 29 121 1663 801 516 128 568 1143
Cuts at: 5108 6165 8278 139
Size: 1057 2113 3119
Fragments arranged by size:
3119 2113 1663 1143 1057 801 568 516
128 121 29
XmnI GAAnn'nnTTC
Cuts at: 2482 5146 2482
Size: 2664 8594
ZraI GAC'GTC
Cuts at: 4053 7823 4053
Size: 3770 7488
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BaeI BmtI BsiWI BssHII HindIII HpaI NdeI
NheI NotI NruI PacI PsiI PspXI PsrI SnaBI
SspI SwaI XhoI