Restriction map for
pF1KE9419
(Circular) (Six-base) MAPSORT of: pF1KE9419.seq Check: 9208 from: 1 to: 4520
pF1KE9419 4520 bp
With 182 enzymes: SgfI *
September 21, 2017 11:26 ..
AatII G_ACGT'C
Cuts at: 595 595
Size: 4520
AccI GT'mk_AC
Cuts at: 1211 1469 1558 1211
Size: 258 89 4173
Fragments arranged by size:
4173 258 89
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 691 1167 2345 2777 3524 3792 165
Size: 526 476 1178 432 747 268 893
Fragments arranged by size:
1178 893 747 526 476 432 268
AflIII A'CryG_T
Cuts at: 227 602 1214 1872 2976 3953 227
Size: 375 612 658 1104 977 794
Fragments arranged by size:
1104 977 794 658 612 375
AgeI A'CCGG_T
Cuts at: 2908 2908
Size: 4520
AlwNI CAG_nnn'CTG
Cuts at: 737 1398 1825 3392 737
Size: 661 427 1567 1865
Fragments arranged by size:
1865 1567 661 427
ApaLI G'TGCA_C
Cuts at: 3290 3290
Size: 4520
ApoI r'AATT_y
Cuts at: 1524 3873 4129 1524
Size: 2349 256 1915
Fragments arranged by size:
2349 1915 256
AscI GG'CGCG_CC
Cuts at: 284 284
Size: 4520
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 303 341 686 1540 303
Size: 38 345 854 3283
Fragments arranged by size:
3283 854 345 38
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 1176 1410 1536 1950 2198 2233 1176
Size: 234 126 414 248 35 3463
Fragments arranged by size:
3463 414 248 234 126 35
BanII G_rGCy'C
Cuts at: 1534 2564 1534
Size: 1030 3490
BbeI G_GCGC'C
Cuts at: 1414 1954 2202 1414
Size: 540 248 3732
Fragments arranged by size:
3732 540 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4486
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3179 4520 2418
Size: 761 1341 2418
Fragments arranged by size:
2418 1341 761
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglI GCCn_nnn'nGGC
Cuts at: 787 787
Size: 4520
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 1181 2145 2238 3294 1181
Size: 964 93 1056 2407
Fragments arranged by size:
2407 1056 964 93
BmrI ACTGGGnnnn_n'
Cuts at: 381 794 1732 1813 2148 4292 381
Size: 413 938 81 335 2144 609
Fragments arranged by size:
2144 938 609 413 335 81
BmtI G_CTAG'C
Cuts at: 172 172
Size: 4520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 289 1170 223
Size: 66 881 3573
Fragments arranged by size:
3573 881 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 1696 2575 3067 3365 1291
Size: 405 879 492 298 2446
Fragments arranged by size:
2446 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 4224 4224
Size: 4520
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 355 2034 4071 6
Size: 349 1679 2037 455
Fragments arranged by size:
2037 1679 455 349
BsaHI Gr'CG_yC
Cuts at: 592 1041 1411 1460 1951 2199 2901 4397 592
Size: 449 370 49 491 248 702 1496 715
Fragments arranged by size:
1496 715 702 491 449 370 248 49
BsaWI w'CCGG_w
Cuts at: 243 680 1048 1709 2230 2908 3182 3329 3740
Size: 437 368 661 521 678 274 147 411
Cuts at: 3740 243
Size: 1023
Fragments arranged by size:
1023 678 661 521 437 411 368 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 853 853
Size: 4520
BseYI C'CCAG_C
Cuts at: 261 873 897 927 945 1498 1945 2703 3280
Size: 612 24 30 18 553 447 758 577
Cuts at: 3280 3776 261
Size: 496 1005
Fragments arranged by size:
1005 758 612 577 553 496 447 30
24 18
BsiEI CG_ry'CG
Cuts at: 104 699 717 996 1049 1427 2062 2108 3316
Size: 595 18 279 53 378 635 46 1208
Cuts at: 3316 104
Size: 1308
Fragments arranged by size:
1308 1208 635 595 378 279 53 46
18
BsiHKAI G_wGCw'C
Cuts at: 506 1385 1534 2312 2502 3294 506
Size: 879 149 778 190 792 1732
Fragments arranged by size:
1732 879 792 778 190 149
BsmBI CGTCTCn'nnnn_
Cuts at: 1337 1337
Size: 4520
Bsp1286I G_dGCh'C
Cuts at: 506 1181 1385 1534 2145 2238 2312 2502 2564
Size: 675 204 149 611 93 74 190 62
Cuts at: 2564 3294 506
Size: 730 1732
Fragments arranged by size:
1732 730 675 611 204 190 149 93
74 62
BspEI T'CCGG_A
Cuts at: 243 3740 243
Size: 3497 1023
BspHI T'CATG_A
Cuts at: 3696 3696
Size: 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 413 977 1448 1499 1570 1784 2086 2467 413
Size: 564 471 51 71 214 302 381 2466
Fragments arranged by size:
2466 564 471 381 302 214 71 51
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2432 2432
Size: 4520
BsrFI r'CCGG_y
Cuts at: 2518 2699 2908 2518
Size: 181 209 4130
Fragments arranged by size:
4130 209 181
BssHII G'CGCG_C
Cuts at: 284 284
Size: 4520
BssSI C'ACGA_G
Cuts at: 216 1279 1384 2791 3149 216
Size: 1063 105 1407 358 1587
Fragments arranged by size:
1587 1407 1063 358 105
BstAPI GCAn_nnn'nTGC
Cuts at: 921 1897 921
Size: 976 3544
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstEII G'GTnAC_C
Cuts at: 1077 1077
Size: 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4520
BstYI r'GATC_y
Cuts at: 1 767 1545 2012 2035 2370 2616 3628 3714
Size: 766 778 467 23 335 246 1012 86
Cuts at: 3714 3726 4072 1
Size: 12 346 449
Fragments arranged by size:
1012 778 766 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1212 1212
Size: 4520
BtgI C'CryG_G
Cuts at: 584 818 1025 1427 584
Size: 234 207 402 3677
Fragments arranged by size:
3677 402 234 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 972 1311 2470 2651 2814 972
Size: 339 1159 181 163 2678
Fragments arranged by size:
2678 1159 339 181 163
BtsI GCAGTG_nn'
Cuts at: 626 1763 626
Size: 1137 3383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 201 236 201
Size: 35 4485
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3084 2226
Size: 858 3662
EaeI y'GGCC_r
Cuts at: 116 209 696 714 746 779 1424 2105 2279
Size: 93 487 18 32 33 645 681 174
Cuts at: 2279 2670 2697 4042 116
Size: 391 27 1345 594
Fragments arranged by size:
1345 681 645 594 487 391 174 93
33 32 27 18
EagI C'GGCC_G
Cuts at: 696 714 1424 2105 696
Size: 18 710 681 3111
Fragments arranged by size:
3111 710 681 18
EarI CTCTTCn'nnn_
Cuts at: 184 421 469 667 2543 2753 184
Size: 237 48 198 1876 210 1951
Fragments arranged by size:
1951 1876 237 210 198 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3038 3184 4330 2728
Size: 310 146 1146 2918
Fragments arranged by size:
2918 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1532 1532
Size: 4520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 223 289 691 1167 1170 2345 2777 3524
Size: 58 66 402 476 3 1175 432 747
Cuts at: 3524 3792 165
Size: 268 893
Fragments arranged by size:
1175 893 747 476 432 402 268 66
58 3
EcoNI CCTnn'n_nnAGG
Cuts at: 202 1204 202
Size: 1002 3518
EcoO109I rG'GnC_Cy
Cuts at: 1404 1659 1404
Size: 255 4265
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4520
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 484 757 1061 1141 1306 1414 1485 1954 2202
Size: 273 304 80 165 108 71 469 248
Cuts at: 2202 3224 4203 484
Size: 1022 979 801
Fragments arranged by size:
1022 979 801 469 304 273 248 165
108 80 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2362 2392 2394 2424 2362
Size: 30 2 30 4458
Fragments arranged by size:
4458 30 30 2
HincII GTy'rAC
Cuts at: 1559 1559
Size: 4520
KasI G'GCGC_C
Cuts at: 1410 1950 2198 1410
Size: 540 248 3732
Fragments arranged by size:
3732 540 248
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 227 1214 3953 227
Size: 987 2739 794
Fragments arranged by size:
2739 987 794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1271 3191 3375 1271
Size: 1920 184 2416
Fragments arranged by size:
2416 1920 184
MscI TGG'CCA
Cuts at: 2281 2281
Size: 4520
MslI CAynn'nnrTG
Cuts at: 112 361 601 631 637 1348 2509 2636 112
Size: 249 240 30 6 711 1161 127 1996
Fragments arranged by size:
1996 1161 711 249 240 127 30 6
MspA1I CmG'CkG
Cuts at: 311 734 945 1027 1253 1429 1475 1631 1945
Size: 423 211 82 226 176 46 156 314
Cuts at: 1945 2305 3318 3563 311
Size: 360 1013 245 1268
Fragments arranged by size:
1268 1013 423 360 314 245 226 211
176 156 82 46
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1411 1951 2199 1411
Size: 540 248 3732
Fragments arranged by size:
3732 540 248
NcoI C'CATG_G
Cuts at: 584 584
Size: 4520
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NheI G'CTAG_C
Cuts at: 168 168
Size: 4520
NotI GC'GGCC_GC
Cuts at: 1424 1424
Size: 4520
NspI r_CATG'y
Cuts at: 435 606 1353 1573 2073 2980 435
Size: 171 747 220 500 907 1975
Fragments arranged by size:
1975 907 747 500 220 171
PasI CC'CwG_GG
Cuts at: 387 387
Size: 4520
PciI A'CATG_T
Cuts at: 602 2976 602
Size: 2374 2146
PflMI CCAn_nnn'nTGG
Cuts at: 657 657
Size: 4520
PfoI T'CCnGG_A
Cuts at: 191 860 191
Size: 669 3851
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4488
PstI C_TGCA'G
Cuts at: 239 1403 1567 2252 239
Size: 1164 164 685 2507
Fragments arranged by size:
2507 1164 685 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 734 1253 1945 2305 734
Size: 519 692 360 2949
Fragments arranged by size:
2949 692 519 360
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 1534 1534
Size: 4520
SacII CC_GC'GG
Cuts at: 1028 1430 1028
Size: 402 4118
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 421 667 2543 2753 421
Size: 246 1876 210 2188
Fragments arranged by size:
2188 1876 246 210
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 235 1399 1470 1563 2248 3241 3432 33
Size: 202 1164 71 93 685 993 191 1121
Fragments arranged by size:
1164 1121 993 685 202 191 93 71
SfiI GGCCn_nnn'nGGCC
Cuts at: 787 787
Size: 4520
SfoI GGC'GCC
Cuts at: 1412 1952 2200 1412
Size: 540 248 3732
Fragments arranged by size:
3732 540 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 305 343 1542 305
Size: 38 1199 3283
Fragments arranged by size:
3283 1199 38
SmlI C'TyrA_G
Cuts at: 1306 1675 2590 3082 3344 1306
Size: 369 915 492 262 2482
Fragments arranged by size:
2482 915 492 369 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 435 1353 1573 2073 435
Size: 918 220 500 2882
Fragments arranged by size:
2882 918 500 220
StyI C'CwwG_G
Cuts at: 584 749 1654 2008 584
Size: 165 905 354 3096
Fragments arranged by size:
3096 905 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 363 2103 2236 2695 2904 363
Size: 1740 133 459 209 1979
Fragments arranged by size:
1979 1740 459 209 133
TatI w'GTAC_w
Cuts at: 368 4035 368
Size: 3667 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 238 270 4037 4069 238
Size: 32 3767 32 689
Fragments arranged by size:
3767 689 32 32
Tth111I GACn'n_nGTC
Cuts at: 2317 2317
Size: 4520
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2015 2015
Size: 4520
XmaI C'CCGG_G
Cuts at: 303 341 1540 303
Size: 38 1199 3283
Fragments arranged by size:
3283 1199 38
ZraI GAC'GTC
Cuts at: 593 593
Size: 4520
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BbsI BbvCI BfrBI BmgBI Bpu10I BsaXI
BsgI BsiWI BsmI BsrGI Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI