Restriction map for
pF1KE9442
(Circular) (Six-base) MAPSORT of: pF1KE9442.seq Check: 716 from: 1 to: 6014
pF1KE9442 6014 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 380 2935 380
Size: 2555 3459
AccI GT'mk_AC
Cuts at: 3052 3052
Size: 6014
Acc65I G'GTAC_C
Cuts at: 3030 3030
Size: 6014
AclI AA'CG_TT
Cuts at: 5854 5854
Size: 6014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1272 1471 1619 2777 3839 4271 5018 5286
Size: 938 199 148 1158 1062 432 747 268
Cuts at: 5286 334
Size: 1062
Fragments arranged by size:
1158 1062 1062 938 747 432 268 199
148
AflIII A'CryG_T
Cuts at: 719 3003 3366 4470 5447 719
Size: 2284 363 1104 977 1286
Fragments arranged by size:
2284 1286 1104 977 363
AgeI A'CCGG_T
Cuts at: 4402 4402
Size: 6014
AhdI GACnn_n'nnGTC
Cuts at: 1985 1985
Size: 6014
AleI CACnn'nnGTG
Cuts at: 1730 1730
Size: 6014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1147 2121 1147
Size: 974 5040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 880 881 912 913 880
Size: 1 31 1 5981
Fragments arranged by size:
5981 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 534 780 891 1017 1686 2715 3319 4886 534
Size: 246 111 126 669 1029 604 1567 1662
Fragments arranged by size:
1662 1567 1029 669 604 246 126 111
ApaI G_GGCC'C
Cuts at: 203 651 2657 2835 203
Size: 448 2006 178 3382
Fragments arranged by size:
3382 2006 448 178
ApaLI G'TGCA_C
Cuts at: 4784 4784
Size: 6014
ApoI r'AATT_y
Cuts at: 3018 5367 5623 3018
Size: 2349 256 3409
Fragments arranged by size:
3409 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6014
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
AvaI C'yCGr_G
Cuts at: 195 271 2521 3034 195
Size: 76 2250 513 3175
Fragments arranged by size:
3175 2250 513 76
AvrII C'CTAG_G
Cuts at: 2178 2178
Size: 6014
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2569 2602 2569
Size: 33 5981
BamHI G'GATC_C
Cuts at: 1514 3039 1514
Size: 1525 4489
BanI G'GyrC_C
Cuts at: 178 1048 1084 1524 1924 2353 3030 3444 3692
Size: 870 36 440 400 429 677 414 248
Cuts at: 3692 3727 178
Size: 35 2465
Fragments arranged by size:
2465 870 677 440 429 414 400 248
36 35
BanII G_rGCy'C
Cuts at: 128 203 272 651 1303 1697 2010 2459 2657
Size: 75 69 379 652 394 313 449 198
Cuts at: 2657 2695 2835 3028 4058 128
Size: 38 140 193 1030 2084
Fragments arranged by size:
2084 1030 652 449 394 379 313 198
193 140 75 69 38
BbeI G_GCGC'C
Cuts at: 182 1928 3448 3696 182
Size: 1746 1520 248 2500
Fragments arranged by size:
2500 1746 1520 248
BbsI GAAGACnn'nnnn_
Cuts at: 1607 1619 1607
Size: 12 6002
BbvCI CC'TCA_GC
Cuts at: 325 2482 2920 325
Size: 2157 438 3419
Fragments arranged by size:
3419 2157 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5980
BciVI GTATCCnnnnn_n'
Cuts at: 3912 4673 6014 3912
Size: 761 1341 3912
Fragments arranged by size:
3912 1341 761
BclI T'GATC_A
Cuts at: 3534 3534
Size: 6014
BfrBI ATG'CAT
Cuts at: 1446 1446
Size: 6014
BglI GCCn_nnn'nGGC
Cuts at: 214 673 1813 2775 214
Size: 459 1140 962 3453
Fragments arranged by size:
3453 1140 962 459
BglII A'GATC_T
Cuts at: 1074 3529 5566 1074
Size: 2455 2037 1522
Fragments arranged by size:
2455 2037 1522
BlpI GC'TnA_GC
Cuts at: 1297 3126 1297
Size: 1829 4185
Bme1580I G_kGCm'C
Cuts at: 203 651 2657 2835 3639 3732 4788 203
Size: 448 2006 178 804 93 1056 1429
Fragments arranged by size:
2006 1429 1056 804 448 178 93
BmrI ACTGGGnnnn_n'
Cuts at: 901 1724 3226 3307 3642 5786 901
Size: 823 1502 81 335 2144 1129
Fragments arranged by size:
2144 1502 1129 823 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1377 2709 4381 1377
Size: 1332 1672 3010
Fragments arranged by size:
3010 1672 1332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1117 1540 1681 1723 1736 2704 2800 2863
Size: 282 423 141 42 13 968 96 63
Cuts at: 2863 835
Size: 3986
Fragments arranged by size:
3986 968 423 282 141 96 63 42
13
Bpu10I CC'TnA_GC
Cuts at: 325 2482 2920 325
Size: 2157 438 3419
Fragments arranged by size:
3419 2157 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3190 4069 4561 4859 3190
Size: 879 492 298 4345
Fragments arranged by size:
4345 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 448 2776 5718 448
Size: 2328 2942 744
Fragments arranged by size:
2942 2328 744
BsaAI yAC'GTr
Cuts at: 2430 3006 3367 2430
Size: 576 361 5077
Fragments arranged by size:
5077 576 361
BsaBI GATnn'nnATC
Cuts at: 6 2735 3528 5565 6
Size: 2729 793 2037 455
Fragments arranged by size:
2729 2037 793 455
BsaHI Gr'CG_yC
Cuts at: 179 1925 3445 3693 4395 5891 179
Size: 1746 1520 248 702 1496 302
Fragments arranged by size:
1746 1520 1496 702 302 248
BsaWI w'CCGG_w
Cuts at: 866 1337 3203 3724 4402 4676 4823 5234 866
Size: 471 1866 521 678 274 147 411 1646
Fragments arranged by size:
1866 1646 678 521 471 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 2230 2260 541
Size: 30 1659 30 4295
Fragments arranged by size:
4295 1659 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 588 1216 1636 2449 400
Size: 188 628 420 813 3965
Fragments arranged by size:
3965 813 628 420 188
BseYI C'CCAG_C
Cuts at: 112 185 483 1210 1790 2012 2499 2761 3439
Size: 73 298 727 580 222 487 262 678
Cuts at: 3439 4197 4774 5270 112
Size: 758 577 496 856
Fragments arranged by size:
856 758 727 678 580 577 496 487
298 262 222 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 1201 1354 2122 2127 790
Size: 411 153 768 5 4677
Fragments arranged by size:
4677 768 411 153 5
BsiEI CG_ry'CG
Cuts at: 104 2675 3556 3602 4810 104
Size: 2571 881 46 1208 1308
Fragments arranged by size:
2571 1308 1208 881 46
BsiHKAI G_wGCw'C
Cuts at: 272 1303 1697 1838 2695 3028 3806 3996 4788
Size: 1031 394 141 857 333 778 190 792
Cuts at: 4788 272
Size: 1498
Fragments arranged by size:
1498 1031 857 792 778 394 333 190
141
BsmI GAATG_Cn'
Cuts at: 433 1006 433
Size: 573 5441
BsmBI CGTCTCn'nnnn_
Cuts at: 400 2011 2118 2124 400
Size: 1611 107 6 4290
Fragments arranged by size:
4290 1611 107 6
Bsp1286I G_dGCh'C
Cuts at: 128 203 272 651 1303 1697 1838 2010 2459
Size: 75 69 379 652 394 141 172 449
Cuts at: 2459 2657 2695 2835 3028 3639 3732 3806 3996
Size: 198 38 140 193 611 93 74 190
Cuts at: 3996 4058 4788 128
Size: 62 730 1354
Fragments arranged by size:
1354 730 652 611 449 394 379 198
193 190 172 141 140 93 75 74
69 62 38
BspEI T'CCGG_A
Cuts at: 866 5234 866
Size: 4368 1646
BspHI T'CATG_A
Cuts at: 5190 5190
Size: 6014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 1025 2387 2597 2935 3064 3278 3580 3961
Size: 645 1362 210 338 129 214 302 381
Cuts at: 3961 380
Size: 2433
Fragments arranged by size:
2433 1362 645 381 338 302 214 210
129
BsrBI CCG'CTC
Cuts at: 50 4306 4360 5844 6009 50
Size: 4256 54 1484 165 55
Fragments arranged by size:
4256 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1848 3926 1848
Size: 2078 3936
BsrFI r'CCGG_y
Cuts at: 172 2350 4012 4193 4402 172
Size: 2178 1662 181 209 1784
Fragments arranged by size:
2178 1784 1662 209 181
BssSI C'ACGA_G
Cuts at: 4285 4643 4285
Size: 358 5656
BstAPI GCAn_nnn'nTGC
Cuts at: 534 3391 534
Size: 2857 3157
BstBI TT'CG_AA
Cuts at: 4375 4375
Size: 6014
BstXI CCAn_nnnn'nTGG
Cuts at: 684 744 1095 684
Size: 60 351 5603
Fragments arranged by size:
5603 351 60
BstYI r'GATC_y
Cuts at: 1 635 1074 1100 1317 1514 2736 2932 3039
Size: 634 439 26 217 197 1222 196 107
Cuts at: 3039 3506 3529 3864 4110 5122 5208 5220 5566
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5566 1
Size: 449
Fragments arranged by size:
1222 1012 634 467 449 439 346 335
246 217 197 196 107 86 26 23
12
Bsu36I CC'TnA_GG
Cuts at: 1352 2276 1352
Size: 924 5090
BtgI C'CryG_G
Cuts at: 1052 2306 2383 2417 2471 2900 1052
Size: 1254 77 34 54 429 4166
Fragments arranged by size:
4166 1254 429 77 54 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3964 4145 4308 3964
Size: 181 163 5670
Fragments arranged by size:
5670 181 163
BtsI GCAGTG_nn'
Cuts at: 225 1414 1594 2105 2110 2212 2578 3257 225
Size: 1189 180 511 5 102 366 679 2982
Fragments arranged by size:
2982 1189 679 511 366 180 102 5
DraI TTT'AAA
Cuts at: 3013 3013
Size: 6014
DrdI GACnn_nn'nnGTC
Cuts at: 3720 4578 3720
Size: 858 5156
EaeI y'GGCC_r
Cuts at: 860 1805 2093 2204 2348 2672 3599 3773 4164
Size: 945 288 111 144 324 927 174 391
Cuts at: 4164 4191 5536 860
Size: 27 1345 1338
Fragments arranged by size:
1345 1338 945 927 391 324 288 174
144 111 27
EagI C'GGCC_G
Cuts at: 2672 3599 2672
Size: 927 5087
EarI CTCTTCn'nnn_
Cuts at: 367 2512 2599 2683 4037 4247 367
Size: 2145 87 84 1354 210 2134
Fragments arranged by size:
2145 2134 1354 210 87 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 270 589 1789 4222 4532 4678 5824 270
Size: 319 1200 2433 310 146 1146 460
Fragments arranged by size:
2433 1200 1146 460 319 310 146
EcoICRI GAG'CTC
Cuts at: 270 1301 1695 2693 3026 270
Size: 1031 394 998 333 3258
Fragments arranged by size:
3258 1031 998 394 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 835 1117 1272 1471 1540 1619 1681 1723
Size: 501 282 155 199 69 79 62 42
Cuts at: 1723 1736 2704 2777 2800 2863 3839 4271 5018
Size: 13 968 73 23 63 976 432 747
Cuts at: 5018 5286 334
Size: 268 1062
Fragments arranged by size:
1062 976 968 747 501 432 282 268
199 155 79 73 69 63 62 42
23 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1751 1751
Size: 6014
EcoO109I rG'GnC_Cy
Cuts at: 199 294 647 648 785 1637 1683 1756 2175
Size: 95 353 1 137 852 46 73 419
Cuts at: 2175 2243 2653 2654 2712 2831 2832 3153 199
Size: 68 410 1 58 119 1 321 3060
Fragments arranged by size:
3060 852 419 410 353 321 137 119
95 73 68 58 46 1 1 1
EcoRI G'AATT_C
Cuts at: 3018 3018
Size: 6014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1483 4033 1483
Size: 2550 3464
FspI TGC'GCA
Cuts at: 3795 3795
Size: 6014
HaeII r_GCGC'y
Cuts at: 136 182 508 1928 3448 3696 4718 5697 136
Size: 46 326 1420 1520 248 1022 979 453
Fragments arranged by size:
1520 1420 1022 979 453 326 248 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1609 1641 1971 2003 2159 2191 3856
Size: 32 1003 32 330 32 156 32 1665
Cuts at: 3856 3886 3888 3918 574
Size: 30 2 30 2670
Fragments arranged by size:
2670 1665 1003 330 156 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3053 3053
Size: 6014
HindIII A'AGCT_T
Cuts at: 2750 2750
Size: 6014
KasI G'GCGC_C
Cuts at: 178 1924 3444 3692 178
Size: 1746 1520 248 2500
Fragments arranged by size:
2500 1746 1520 248
KpnI G_GTAC'C
Cuts at: 3034 3034
Size: 6014
MluI A'CGCG_T
Cuts at: 719 5447 719
Size: 4728 1286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1275 2045 4685 4869 1275
Size: 770 2640 184 2420
Fragments arranged by size:
2640 2420 770 184
MscI TGG'CCA
Cuts at: 862 3775 862
Size: 2913 3101
MslI CAynn'nnrTG
Cuts at: 971 1613 1730 4003 4130 971
Size: 642 117 2273 127 2855
Fragments arranged by size:
2855 2273 642 127 117
MspA1I CmG'CkG
Cuts at: 483 1158 1296 1407 1701 2012 2385 3125 3439
Size: 675 138 111 294 311 373 740 314
Cuts at: 3439 3799 4812 5057 483
Size: 360 1013 245 1440
Fragments arranged by size:
1440 1013 740 675 373 360 314 311
294 245 138 111
NaeI GCC'GGC
Cuts at: 174 4195 174
Size: 4021 1993
NarI GG'CG_CC
Cuts at: 179 1925 3445 3693 179
Size: 1746 1520 248 2500
Fragments arranged by size:
2500 1746 1520 248
NcoI C'CATG_G
Cuts at: 1052 2417 2900 1052
Size: 1365 483 4166
Fragments arranged by size:
4166 1365 483
NgoMIV G'CCGG_C
Cuts at: 172 4193 172
Size: 4021 1993
NsiI A_TGCA'T
Cuts at: 1448 1448
Size: 6014
NspI r_CATG'y
Cuts at: 3067 3567 4474 3067
Size: 500 907 4607
Fragments arranged by size:
4607 907 500
PasI CC'CwG_GG
Cuts at: 873 1641 2154 2295 2773 873
Size: 768 513 141 478 4114
Fragments arranged by size:
4114 768 513 478 141
PciI A'CATG_T
Cuts at: 4470 4470
Size: 6014
PflMI CCAn_nnn'nTGG
Cuts at: 780 993 1686 2715 2947 780
Size: 213 693 1029 232 3847
Fragments arranged by size:
3847 1029 693 232 213
PfoI T'CCnGG_A
Cuts at: 163 566 1061 1175 1517 2609 163
Size: 403 495 114 342 1092 3568
Fragments arranged by size:
3568 1092 495 403 342 114
PmeI GTTT'AAAC
Cuts at: 3013 3013
Size: 6014
PmlI CAC'GTG
Cuts at: 2430 3006 2430
Size: 576 5438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 397 429 5153 5185 397
Size: 32 4724 32 1226
Fragments arranged by size:
4724 1226 32 32
PpuMI rG'GwC_Cy
Cuts at: 294 785 1683 2175 294
Size: 491 898 492 4133
Fragments arranged by size:
4133 898 492 491
PsiI TTA'TAA
Cuts at: 836 836
Size: 6014
PspOMI G'GGCC_C
Cuts at: 199 647 2653 2831 199
Size: 448 2006 178 3382
Fragments arranged by size:
3382 2006 448 178
PstI C_TGCA'G
Cuts at: 473 530 674 848 887 3061 3746 473
Size: 57 144 174 39 2174 685 2741
Fragments arranged by size:
2741 2174 685 174 144 57 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6014
PvuII CAG'CTG
Cuts at: 483 1158 1296 1407 1701 3439 3799 483
Size: 675 138 111 294 1738 360 2698
Fragments arranged by size:
2698 1738 675 360 294 138 111
RsrII CG'GwC_CG
Cuts at: 4209 4209
Size: 6014
SacI G_AGCT'C
Cuts at: 272 1303 1697 2695 3028 272
Size: 1031 394 998 333 3258
Fragments arranged by size:
3258 1031 998 394 333
SacII CC_GC'GG
Cuts at: 2386 2386
Size: 6014
SalI G'TCGA_C
Cuts at: 3051 3051
Size: 6014
SanDI GG'GwC_CC
Cuts at: 785 785
Size: 6014
SapI GCTCTTCn'nnn_
Cuts at: 2599 2683 4037 4247 2599
Size: 84 1354 210 4366
Fragments arranged by size:
4366 1354 210 84
SbfI CC_TGCA'GG
Cuts at: 674 3061 674
Size: 2387 3627
ScaI AGT'ACT
Cuts at: 2057 5531 2057
Size: 3474 2540
SfcI C'TryA_G
Cuts at: 33 447 469 526 670 844 883 955 3057
Size: 414 22 57 144 174 39 72 2102
Cuts at: 3057 3742 4735 4926 33
Size: 685 993 191 1121
Fragments arranged by size:
2102 1121 993 685 414 191 174 144
72 57 39 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 1813 1813
Size: 6014
SfoI GGC'GCC
Cuts at: 180 1926 3446 3694 180
Size: 1746 1520 248 2500
Fragments arranged by size:
2500 1746 1520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
SmaI CCC'GGG
Cuts at: 197 3036 197
Size: 2839 3175
SmlI C'TyrA_G
Cuts at: 3169 4084 4576 4838 3169
Size: 915 492 262 4345
Fragments arranged by size:
4345 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6014
SphI G_CATG'C
Cuts at: 3067 3567 3067
Size: 500 5514
StuI AGG'CCT
Cuts at: 934 1357 1483 1579 934
Size: 423 126 96 5369
Fragments arranged by size:
5369 423 126 96
StyI C'CwwG_G
Cuts at: 1052 1490 2178 2417 2900 3148 3502 1052
Size: 438 688 239 483 248 354 3564
Fragments arranged by size:
3564 688 483 438 354 248 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3597 3730 4189 4398 3597
Size: 133 459 209 5213
Fragments arranged by size:
5213 459 209 133
TatI w'GTAC_w
Cuts at: 2055 2369 5529 2055
Size: 314 3160 2540
Fragments arranged by size:
3160 2540 314
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5531 5563 5531
Size: 32 5982
Tth111I GACn'n_nGTC
Cuts at: 3811 3811
Size: 6014
XbaI T'CTAG_A
Cuts at: 3045 3045
Size: 6014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 870 1419 2046 3509 870
Size: 549 627 1463 3375
Fragments arranged by size:
3375 1463 627 549
XmaI C'CCGG_G
Cuts at: 195 3034 195
Size: 2839 3175
XmnI GAAnn'nnTTC
Cuts at: 1604 2329 1604
Size: 725 5289
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI BmgBI BmtI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PshAI PspXI PsrI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI