Restriction map for
pF1KE9532
(Circular) (Six-base) MAPSORT of: pF1KE9532.seq Check: 3014 from: 1 to: 4223
pF1KE9532 4223 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1128 1128
Size: 4223
AatII G_ACGT'C
Cuts at: 590 590
Size: 4223
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1203 1239 1203
Size: 36 4187
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 2048 2480 3227 3495 145
Size: 1903 432 747 268 873
Fragments arranged by size:
1903 873 747 432 268
AflIII A'CryG_T
Cuts at: 506 1575 2679 3656 506
Size: 1069 1104 977 1073
Fragments arranged by size:
1104 1073 1069 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AleI CACnn'nnGTG
Cuts at: 268 268
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 1528 3095 1528
Size: 1567 2656
ApaI G_GGCC'C
Cuts at: 636 745 636
Size: 109 4114
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 1227 3576 3832 1227
Size: 2349 256 1618
Fragments arranged by size:
2349 1618 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 250 852 860 1243 250
Size: 602 8 383 3230
Fragments arranged by size:
3230 602 383 8
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 235 1203 1239 1653 1901 1936 235
Size: 968 36 414 248 35 2522
Fragments arranged by size:
2522 968 414 248 36 35
BanII G_rGCy'C
Cuts at: 116 187 257 278 305 636 676 745 859
Size: 71 70 21 27 331 40 69 114
Cuts at: 859 1237 2267 116
Size: 378 1030 2072
Fragments arranged by size:
2072 1030 378 331 114 71 70 69
40 27 21
BbeI G_GCGC'C
Cuts at: 239 1657 1905 239
Size: 1418 248 2557
Fragments arranged by size:
2557 1418 248
BbsI GAAGACnn'nnnn_
Cuts at: 361 942 361
Size: 581 3642
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BglI GCCn_nnn'nGGC
Cuts at: 252 631 252
Size: 379 3844
BglII A'GATC_T
Cuts at: 1738 3775 1738
Size: 2037 2186
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 433 582 636 745 1848 1941 2997 433
Size: 149 54 109 1103 93 1056 1659
Fragments arranged by size:
1659 1103 1056 149 109 93 54
BmgBI CAC'GTC
Cuts at: 596 1134 1173 596
Size: 538 39 3646
Fragments arranged by size:
3646 538 39
BmrI ACTGGGnnnn_n'
Cuts at: 747 1435 1516 1851 3995 747
Size: 688 81 335 2144 975
Fragments arranged by size:
2144 975 688 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4223
Bpu10I CC'TnA_GC
Cuts at: 181 181
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1399 2278 2770 3068 241
Size: 1158 879 492 298 1396
Fragments arranged by size:
1396 1158 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 1576 1576
Size: 4223
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 236 587 937 1654 1902 2604 4100 236
Size: 351 350 717 248 702 1496 359
Fragments arranged by size:
1496 717 702 359 351 350 248
BsaWI w'CCGG_w
Cuts at: 872 1412 1933 2611 2885 3032 3443 872
Size: 540 521 678 274 147 411 1652
Fragments arranged by size:
1652 678 540 521 411 274 147
BseYI C'CCAG_C
Cuts at: 969 1648 2406 2983 3479 969
Size: 679 758 577 496 1713
Fragments arranged by size:
1713 758 679 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1156 1002
Size: 154 4069
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 794 1237 2015 2205 2997 794
Size: 443 778 190 792 2020
Fragments arranged by size:
2020 792 778 443 190
BsmI GAATG_Cn'
Cuts at: 471 471
Size: 4223
BsmBI CGTCTCn'nnnn_
Cuts at: 1192 1192
Size: 4223
Bsp1286I G_dGCh'C
Cuts at: 116 187 257 278 305 433 582 636 676
Size: 71 70 21 27 128 149 54 40
Cuts at: 676 745 794 859 1237 1848 1941 2015 2205
Size: 69 49 65 378 611 93 74 190
Cuts at: 2205 2267 2997 116
Size: 62 730 1342
Fragments arranged by size:
1342 730 611 378 190 149 128 93
74 71 70 69 65 62 54 49
40 27 21
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 818 3399 818
Size: 2581 1642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 887 1128 1273 1487 1789 2170 887
Size: 241 145 214 302 381 2940
Fragments arranged by size:
2940 381 302 241 214 145
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 485 1160 2135 485
Size: 675 975 2573
Fragments arranged by size:
2573 975 675
BsrFI r'CCGG_y
Cuts at: 2221 2402 2611 2221
Size: 181 209 3833
Fragments arranged by size:
3833 209 181
BssSI C'ACGA_G
Cuts at: 2494 2852 2494
Size: 358 3865
BstAPI GCAn_nnn'nTGC
Cuts at: 216 1106 1600 216
Size: 890 494 2839
Fragments arranged by size:
2839 890 494
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 261 261
Size: 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 543 1173 543
Size: 630 3593
BstYI r'GATC_y
Cuts at: 1 716 1248 1715 1738 2073 2319 3331 3417
Size: 715 532 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 715 532 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 337 728 337
Size: 391 3832
BtgI C'CryG_G
Cuts at: 107 231 834 998 1207 107
Size: 124 603 164 209 3123
Fragments arranged by size:
3123 603 209 164 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1466
Size: 4223
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 403 1929 2787 403
Size: 1526 858 1839
Fragments arranged by size:
1839 1526 858
EaeI y'GGCC_r
Cuts at: 284 392 454 623 1001 1808 1982 2373 2400
Size: 108 62 169 378 807 174 391 27
Cuts at: 2400 3745 284
Size: 1345 762
Fragments arranged by size:
1345 807 762 391 378 174 169 108
62 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 124 689 775 2246 2456 124
Size: 565 86 1471 210 1891
Fragments arranged by size:
1891 1471 565 210 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 832 2431 2741 2887 4033 832
Size: 1599 310 146 1146 1022
Fragments arranged by size:
1599 1146 1022 310 146
EcoICRI GAG'CTC
Cuts at: 1235 1235
Size: 4223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 2048 2480 3227 3495 145
Size: 1903 432 747 268 873
Fragments arranged by size:
1903 873 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 633 741 1362 633
Size: 108 621 3494
Fragments arranged by size:
3494 621 108
EcoRI G'AATT_C
Cuts at: 1227 1227
Size: 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1110 2242 1110
Size: 1132 3091
FspI TGC'GCA
Cuts at: 1156 2004 1156
Size: 848 3375
HaeII r_GCGC'y
Cuts at: 239 1657 1905 2927 3906 239
Size: 1418 248 1022 979 556
Fragments arranged by size:
1418 1022 979 556 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 531 563 2065 2095 2097 2127 531
Size: 32 1502 30 2 30 2627
Fragments arranged by size:
2627 1502 32 30 30 2
HincII GTy'rAC
Cuts at: 1038 1262 1038
Size: 224 3999
KasI G'GCGC_C
Cuts at: 235 1653 1901 235
Size: 1418 248 2557
Fragments arranged by size:
2557 1418 248
KpnI G_GTAC'C
Cuts at: 1207 1243 1207
Size: 36 4187
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 276 2894 3078 276
Size: 2618 184 1421
Fragments arranged by size:
2618 1421 184
MscI TGG'CCA
Cuts at: 625 1003 1984 625
Size: 378 981 2864
Fragments arranged by size:
2864 981 378
MslI CAynn'nnrTG
Cuts at: 268 349 541 2212 2339 268
Size: 81 192 1671 127 2152
Fragments arranged by size:
2152 1671 192 127 81
MspA1I CmG'CkG
Cuts at: 1043 1209 1334 1648 2008 3021 3266 1043
Size: 166 125 314 360 1013 245 2000
Fragments arranged by size:
2000 1013 360 314 245 166 125
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 236 1654 1902 236
Size: 1418 248 2557
Fragments arranged by size:
2557 1418 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4223
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NspI r_CATG'y
Cuts at: 510 1276 1776 2683 510
Size: 766 500 907 2050
Fragments arranged by size:
2050 907 766 500
PasI CC'CwG_GG
Cuts at: 1026 1026
Size: 4223
PciI A'CATG_T
Cuts at: 506 2679 506
Size: 2173 2050
PflMI CCAn_nnn'nTGG
Cuts at: 969 969
Size: 4223
PfoI T'CCnGG_A
Cuts at: 1070 1070
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PshAI GACnn'nnGTC
Cuts at: 940 940
Size: 4223
PspOMI G'GGCC_C
Cuts at: 632 741 632
Size: 109 4114
PstI C_TGCA'G
Cuts at: 1270 1955 1270
Size: 685 3538
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 1043 1648 2008 1043
Size: 605 360 3258
Fragments arranged by size:
3258 605 360
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1237 1237
Size: 4223
SacII CC_GC'GG
Cuts at: 1210 1210
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 810 1266 1951 2944 3135 33
Size: 777 456 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 456 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 631 631
Size: 4223
SfoI GGC'GCC
Cuts at: 237 1655 1903 237
Size: 1418 248 2557
Fragments arranged by size:
2557 1418 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 256 1378 2293 2785 3047 256
Size: 1122 915 492 262 1432
Fragments arranged by size:
1432 1122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
SspI AAT'ATT
Cuts at: 369 369
Size: 4223
StuI AGG'CCT
Cuts at: 1090 1090
Size: 4223
StyI C'CwwG_G
Cuts at: 107 963 1166 1357 1711 107
Size: 856 203 191 354 2619
Fragments arranged by size:
2619 856 354 203 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1806 1939 2398 2607 1806
Size: 133 459 209 3422
Fragments arranged by size:
3422 459 209 133
TatI w'GTAC_w
Cuts at: 3738 3738
Size: 4223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4191
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 633 1718 633
Size: 1085 3138
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
ZraI GAC'GTC
Cuts at: 588 588
Size: 4223
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BpmI BsaXI BseRI BsiWI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PsiI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SwaI XhoI XmnI