Restriction map for
pF1KE9573
(Circular) (Six-base) MAPSORT of: pF1KE9573.seq Check: 857 from: 1 to: 4535
pF1KE9573 4535 bp
With 182 enzymes: SgfI *
September 11, 2018 18:37 ..
AccI GT'mk_AC
Cuts at: 1573 1573
Size: 4535
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1144 2360 2792 3539 3807 553
Size: 591 1216 432 747 268 1281
Fragments arranged by size:
1281 1216 747 591 432 268
AflIII A'CryG_T
Cuts at: 665 1115 1887 2991 3968 665
Size: 450 772 1104 977 1232
Fragments arranged by size:
1232 1104 977 772 450
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AleI CACnn'nnGTG
Cuts at: 595 595
Size: 4535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 1399 1446 1840 3407 1399
Size: 47 394 1567 2527
Fragments arranged by size:
2527 1567 394 47
ApaI G_GGCC'C
Cuts at: 1329 1329
Size: 4535
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 687 1337 1539 3888 4144 687
Size: 650 202 2349 256 1078
Fragments arranged by size:
2349 1078 650 256 202
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1555 1555
Size: 4535
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 921 1364 1551 1965 2213 2248 921
Size: 443 187 414 248 35 3208
Fragments arranged by size:
3208 443 414 248 187 35
BanII G_rGCy'C
Cuts at: 215 740 1329 1549 2579 215
Size: 525 589 220 1030 2171
Fragments arranged by size:
2171 1030 589 525 220
BbeI G_GCGC'C
Cuts at: 1368 1969 2217 1368
Size: 601 248 3686
Fragments arranged by size:
3686 601 248
BbvCI CC'TCA_GC
Cuts at: 1447 1447
Size: 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1465 1499 1465
Size: 34 4501
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BfrBI ATG'CAT
Cuts at: 541 541
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 1157 1329 2160 2253 3309 1157
Size: 172 831 93 1056 2383
Fragments arranged by size:
2383 1056 831 172 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1209 389
Size: 820 3715
Bpu10I CC'TnA_GC
Cuts at: 1447 1447
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1711 2590 3082 3380 1711
Size: 879 492 298 2866
Fragments arranged by size:
2866 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1185 4239 1185
Size: 3054 1481
BsaAI yAC'GTr
Cuts at: 1230 1888 1230
Size: 658 3877
BsaBI GATnn'nnATC
Cuts at: 6 413 700 2049 4086 6
Size: 407 287 1349 2037 455
Fragments arranged by size:
2037 1349 455 407 287
BsaHI Gr'CG_yC
Cuts at: 1365 1966 2214 2916 4412 1365
Size: 601 248 702 1496 1488
Fragments arranged by size:
1496 1488 702 601 248
BsaWI w'CCGG_w
Cuts at: 405 1724 2245 2923 3197 3344 3755 405
Size: 1319 521 678 274 147 411 1185
Fragments arranged by size:
1319 1185 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 725 755 725
Size: 30 4505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1492 1492
Size: 4535
BseYI C'CCAG_C
Cuts at: 350 905 1161 1960 2718 3295 3791 350
Size: 555 256 799 758 577 496 1094
Fragments arranged by size:
1094 799 758 577 555 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 640
Size: 4535
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 740 1549 2327 2517 3309 740
Size: 809 778 190 792 1966
Fragments arranged by size:
1966 809 792 778 190
BsmI GAATG_Cn'
Cuts at: 287 543 287
Size: 256 4279
Bsp1286I G_dGCh'C
Cuts at: 215 740 1157 1329 1549 2160 2253 2327 2517
Size: 525 417 172 220 611 93 74 190
Cuts at: 2517 2579 3309 215
Size: 62 730 1441
Fragments arranged by size:
1441 730 611 525 417 220 190 172
93 74 62
BspEI T'CCGG_A
Cuts at: 405 3755 405
Size: 3350 1185
BspHI T'CATG_A
Cuts at: 3711 3711
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1799 2101 2482 1585
Size: 214 302 381 3638
Fragments arranged by size:
3638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1379 2827 2881 4365 4530 50
Size: 1329 1448 54 1484 165 55
Fragments arranged by size:
1484 1448 1329 165 55 54
BsrDI GCAATG_nn'
Cuts at: 516 2447 516
Size: 1931 2604
BsrFI r'CCGG_y
Cuts at: 128 363 2533 2714 2923 128
Size: 235 2170 181 209 1740
Fragments arranged by size:
2170 1740 235 209 181
BssSI C'ACGA_G
Cuts at: 739 2806 3164 739
Size: 2067 358 2110
Fragments arranged by size:
2110 2067 358
BstAPI GCAn_nnn'nTGC
Cuts at: 882 1399 1912 882
Size: 517 513 3505
Fragments arranged by size:
3505 517 513
BstBI TT'CG_AA
Cuts at: 2896 2896
Size: 4535
BstEII G'GTnAC_C
Cuts at: 648 648
Size: 4535
BstYI r'GATC_y
Cuts at: 1 830 1560 2027 2050 2385 2631 3643 3729
Size: 829 730 467 23 335 246 1012 86
Cuts at: 3729 3741 4087 1
Size: 12 346 449
Fragments arranged by size:
1012 829 730 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1011 1011
Size: 4535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 1778 1778
Size: 4535
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3099 2241
Size: 858 3677
EaeI y'GGCC_r
Cuts at: 2120 2294 2685 2712 4057 2120
Size: 174 391 27 1345 2598
Fragments arranged by size:
2598 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1120 2743 3053 3199 4345 1120
Size: 1623 310 146 1146 1310
Fragments arranged by size:
1623 1310 1146 310 146
EcoICRI GAG'CTC
Cuts at: 738 1547 738
Size: 809 3726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 553 1144 1209 2360 2792 3539 3807 389
Size: 164 591 65 1151 432 747 268 1117
Fragments arranged by size:
1151 1117 747 591 432 268 164 65
EcoO109I rG'GnC_Cy
Cuts at: 750 1674 750
Size: 924 3611
EcoRI G'AATT_C
Cuts at: 687 1539 687
Size: 852 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 897 2554 897
Size: 1657 2878
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 1320 1368 1969 2217 3239 4218 1320
Size: 48 601 248 1022 979 1637
Fragments arranged by size:
1637 1022 979 601 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1093 1125 1224 1256 1504 1536 2377 2407 2409
Size: 32 99 32 248 32 841 30 2
Cuts at: 2409 2439 1093
Size: 30 3189
Fragments arranged by size:
3189 841 248 99 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1574 1574
Size: 4535
KasI G'GCGC_C
Cuts at: 1364 1965 2213 1364
Size: 601 248 3686
Fragments arranged by size:
3686 601 248
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 226 518 1223 1397 3206 3390 226
Size: 292 705 174 1809 184 1371
Fragments arranged by size:
1809 1371 705 292 184 174
MscI TGG'CCA
Cuts at: 2296 2296
Size: 4535
MslI CAynn'nnrTG
Cuts at: 595 853 2524 2651 595
Size: 258 1671 127 2479
Fragments arranged by size:
2479 1671 258 127
MspA1I CmG'CkG
Cuts at: 918 1399 1646 1960 2320 3333 3578 918
Size: 481 247 314 360 1013 245 1875
Fragments arranged by size:
1875 1013 481 360 314 247 245
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1365 1966 2214 1365
Size: 601 248 3686
Fragments arranged by size:
3686 601 248
NcoI C'CATG_G
Cuts at: 1011 1011
Size: 4535
NdeI CA'TA_TG
Cuts at: 543 543
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NsiI A_TGCA'T
Cuts at: 543 543
Size: 4535
NspI r_CATG'y
Cuts at: 669 1119 1588 2088 2995 669
Size: 450 469 500 907 2209
Fragments arranged by size:
2209 907 500 469 450
PasI CC'CwG_GG
Cuts at: 606 606
Size: 4535
PciI A'CATG_T
Cuts at: 665 1115 2991 665
Size: 450 1876 2209
Fragments arranged by size:
2209 1876 450
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PpuMI rG'GwC_Cy
Cuts at: 750 750
Size: 4535
PspOMI G'GGCC_C
Cuts at: 1325 1325
Size: 4535
PstI C_TGCA'G
Cuts at: 1395 1582 2267 1395
Size: 187 685 3663
Fragments arranged by size:
3663 685 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 1960 2320 1960
Size: 360 4175
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 740 1549 740
Size: 809 3726
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SanDI GG'GwC_CC
Cuts at: 750 750
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SexAI A'CCwGG_T
Cuts at: 487 710 487
Size: 223 4312
SfcI C'TryA_G
Cuts at: 33 1391 1578 2263 3256 3447 33
Size: 1358 187 685 993 191 1121
Fragments arranged by size:
1358 1121 993 685 191 187
SfoI GGC'GCC
Cuts at: 1366 1967 2215 1366
Size: 601 248 3686
Fragments arranged by size:
3686 601 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 1690 2605 3097 3359 1690
Size: 915 492 262 2866
Fragments arranged by size:
2866 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StyI C'CwwG_G
Cuts at: 303 633 1011 1470 1669 2023 303
Size: 330 378 459 199 354 2815
Fragments arranged by size:
2815 459 378 354 330 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 176 293 2118 2251 2710 2919 176
Size: 117 1825 133 459 209 1792
Fragments arranged by size:
1825 1792 459 209 133 117
TatI w'GTAC_w
Cuts at: 263 551 788 956 4050 263
Size: 288 237 168 3094 748
Fragments arranged by size:
3094 748 288 237 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 725 757 807 839 4052 4084 725
Size: 32 50 32 3213 32 1176
Fragments arranged by size:
3213 1176 50 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 4535
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI