Restriction map for
pF1KE9610
(Circular) (Six-base) MAPSORT of: pF1KE9610.seq Check: 622 from: 1 to: 4010
pF1KE9610 4010 bp
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AccI GT'mk_AC
Cuts at: 245 1048 245
Size: 803 3207
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 772
Size: 32 3978
AlwNI CAG_nnn'CTG
Cuts at: 472 647 1315 2882 472
Size: 175 668 1567 1600
Fragments arranged by size:
1600 1567 668 175
ApaI G_GGCC'C
Cuts at: 226 238 226
Size: 12 3998
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 580 662 1030 580
Size: 82 368 3560
Fragments arranged by size:
3560 368 82
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 226 238 1024 2054 226
Size: 12 786 1030 2182
Fragments arranged by size:
2182 1030 786 12
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BciVI GTATCCnnnnn_n'
Cuts at: 708 1908 2669 4010 708
Size: 1200 761 1341 708
Fragments arranged by size:
1341 1200 761 708
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BfrBI ATG'CAT
Cuts at: 490 490
Size: 4010
BglI GCCn_nnn'nGGC
Cuts at: 658 658
Size: 4010
BglII A'GATC_T
Cuts at: 282 1525 3562 282
Size: 1243 2037 730
Fragments arranged by size:
2037 1243 730
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 170 226 238 1635 1728 2784 170
Size: 56 12 1397 93 1056 1396
Fragments arranged by size:
1397 1396 1056 93 56 12
BmrI ACTGGGnnnn_n'
Cuts at: 482 795 1222 1303 1638 3782 482
Size: 313 427 81 335 2144 710
Fragments arranged by size:
2144 710 427 335 313 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 307 862 1006 145
Size: 162 555 144 3149
Fragments arranged by size:
3149 555 162 144
Bpu10I CC'TnA_GC
Cuts at: 729 729
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 777 1363 777
Size: 586 3424
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 203 1441 1689 2391 3887 203
Size: 1238 248 702 1496 326
Fragments arranged by size:
1496 1238 702 326 248
BsaWI w'CCGG_w
Cuts at: 809 1199 1720 2398 2672 2819 3230 809
Size: 390 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 521 411 390 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 772 802 433
Size: 30 309 30 3641
Fragments arranged by size:
3641 309 30 30
BseYI C'CCAG_C
Cuts at: 231 377 1435 2193 2770 3266 231
Size: 146 1058 758 577 496 975
Fragments arranged by size:
1058 975 758 577 496 146
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 776 1024 1802 1992 2784 776
Size: 248 778 190 792 2002
Fragments arranged by size:
2002 792 778 248 190
Bsp1286I G_dGCh'C
Cuts at: 170 226 238 776 1024 1635 1728 1802 1992
Size: 56 12 538 248 611 93 74 190
Cuts at: 1992 2054 2784 170
Size: 62 730 1396
Fragments arranged by size:
1396 730 611 538 248 190 93 74
62 56 12
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 333 2302 2356 3840 4005 50
Size: 283 1969 54 1484 165 55
Fragments arranged by size:
1969 1484 283 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 111 282 1035 1502 1525 1860 2106 3118
Size: 110 171 753 467 23 335 246 1012
Cuts at: 3118 3204 3216 3562 1
Size: 86 12 346 449
Fragments arranged by size:
1012 753 467 449 346 335 246 171
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 170 239 107
Size: 63 69 3878
Fragments arranged by size:
3878 69 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 1253 1253
Size: 4010
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 1595 1769 2160 2187 3532 1595
Size: 174 391 27 1345 2073
Fragments arranged by size:
2073 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 868 2218 2528 2674 3820 868
Size: 1350 310 146 1146 1058
Fragments arranged by size:
1350 1146 1058 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 307 862 1006 1835 2267 3014 3282 145
Size: 162 555 144 829 432 747 268 873
Fragments arranged by size:
873 829 747 555 432 268 162 144
EcoO109I rG'GnC_Cy
Cuts at: 222 223 235 935 1149 222
Size: 1 12 700 214 3083
Fragments arranged by size:
3083 700 214 12 1
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 295 385 1444 1692 2714 3693 295
Size: 90 1059 248 1022 979 612
Fragments arranged by size:
1059 1022 979 612 248 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 712 744 865 897 1852 1882 1884
Size: 32 301 32 121 32 955 30 2
Cuts at: 1884 1914 379
Size: 30 2475
Fragments arranged by size:
2475 955 301 121 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 1999 2126 1999
Size: 127 3883
MspA1I CmG'CkG
Cuts at: 146 172 818 1121 1435 1795 2808 3053 146
Size: 26 646 303 314 360 1013 245 1103
Fragments arranged by size:
1103 1013 646 360 314 303 245 26
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 239 107
Size: 132 3878
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NsiI A_TGCA'T
Cuts at: 492 492
Size: 4010
NspI r_CATG'y
Cuts at: 423 1063 1563 2470 423
Size: 640 500 907 1963
Fragments arranged by size:
1963 907 640 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PfoI T'CCnGG_A
Cuts at: 839 971 839
Size: 132 3878
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PmlI CAC'GTG
Cuts at: 777 777
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 772 804 3149 3181 772
Size: 32 2345 32 1601
Fragments arranged by size:
2345 1601 32 32
PpuMI rG'GwC_Cy
Cuts at: 935 935
Size: 4010
PspOMI G'GGCC_C
Cuts at: 222 234 222
Size: 12 3998
PstI C_TGCA'G
Cuts at: 569 707 1057 1742 569
Size: 138 350 685 2837
Fragments arranged by size:
2837 685 350 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 818 1435 1795 818
Size: 617 360 3033
Fragments arranged by size:
3033 617 360
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SacII CC_GC'GG
Cuts at: 173 173
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SanDI GG'GwC_CC
Cuts at: 935 935
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 565 703 1053 1738 2731 2922 33
Size: 532 138 350 685 993 191 1121
Fragments arranged by size:
1121 993 685 532 350 191 138
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 582 664 1032 582
Size: 82 368 3560
Fragments arranged by size:
3560 368 82
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 423 1063 1563 423
Size: 640 500 2870
Fragments arranged by size:
2870 640 500
StyI C'CwwG_G
Cuts at: 107 239 653 1144 1498 107
Size: 132 414 491 354 2619
Fragments arranged by size:
2619 491 414 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 1593 1726 2185 2394 529
Size: 1064 133 459 209 2145
Fragments arranged by size:
2145 1064 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 370 1807 370
Size: 1437 2573
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 580 662 1030 580
Size: 82 368 3560
Fragments arranged by size:
3560 368 82
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI