Restriction map for
pF1KE9612
(Circular) (Six-base) MAPSORT of: pF1KE9612.seq Check: 9790 from: 1 to: 4049
pF1KE9612 4049 bp
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AccI GT'mk_AC
Cuts at: 276 1087 276
Size: 811 3238
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1874 2306 3053 3321 803
Size: 1071 432 747 268 1531
Fragments arranged by size:
1531 1071 747 432 268
AflIII A'CryG_T
Cuts at: 155 467 1401 2505 3482 155
Size: 312 934 1104 977 722
Fragments arranged by size:
1104 977 934 722 312
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 376 376
Size: 4049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 285 1354 2921 285
Size: 1069 1567 1413
Fragments arranged by size:
1567 1413 1069
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 668 1069 668
Size: 401 3648
BamHI G'GATC_C
Cuts at: 381 1074 381
Size: 693 3356
BanI G'GyrC_C
Cuts at: 142 1065 1479 1727 1762 142
Size: 923 414 248 35 2429
Fragments arranged by size:
2429 923 414 248 35
BanII G_rGCy'C
Cuts at: 1063 2093 1063
Size: 1030 3019
BbeI G_GCGC'C
Cuts at: 146 1483 1731 146
Size: 1337 248 2464
Fragments arranged by size:
2464 1337 248
BbsI GAAGACnn'nnnn_
Cuts at: 349 598 925 349
Size: 249 327 3473
Fragments arranged by size:
3473 327 249
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 447 1569 447
Size: 1122 2927
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 376 2416 376
Size: 2040 2009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1225 2104 2596 2894 529
Size: 696 879 492 298 1684
Fragments arranged by size:
1684 879 696 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 156 1402 156
Size: 1246 2803
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 143 1480 1728 2430 3926 143
Size: 1337 248 702 1496 266
Fragments arranged by size:
1496 1337 702 266 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 418
Size: 30 4019
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1063 1674 1767 1841 2031 2093 2823 1063
Size: 611 93 74 190 62 730 2289
Fragments arranged by size:
2289 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 826 1961 826
Size: 1135 2914
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstEII G'GTnAC_C
Cuts at: 697 697
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 378 378
Size: 4049
BstYI r'GATC_y
Cuts at: 1 381 1074 1541 1564 1899 2145 3157 3243
Size: 380 693 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 693 467 449 380 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 744 107
Size: 637 3412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 179 1048 179
Size: 869 3180
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 110 1634 1808 2199 2226 3571 110
Size: 1524 174 391 27 1345 588
Fragments arranged by size:
1524 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 716 2072 2282 716
Size: 1356 210 2483
Fragments arranged by size:
2483 1356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1874 2306 3053 3321 803
Size: 1071 432 747 268 1531
Fragments arranged by size:
1531 1071 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 412 412
Size: 4049
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
EcoRV GAT'ATC
Cuts at: 755 755
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 146 1483 1731 2753 3732 146
Size: 1337 248 1022 979 463
Fragments arranged by size:
1337 1022 979 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 936 968 1891 1921 1923 1953 775
Size: 32 129 32 923 30 2 30 2871
Fragments arranged by size:
2871 923 129 32 32 30 30 2
HincII GTy'rAC
Cuts at: 231 680 975 1088 231
Size: 449 295 113 3192
Fragments arranged by size:
3192 449 295 113
HindIII A'AGCT_T
Cuts at: 704 805 704
Size: 101 3948
HpaI GTT'AAC
Cuts at: 231 231
Size: 4049
KasI G'GCGC_C
Cuts at: 142 1479 1727 142
Size: 1337 248 2464
Fragments arranged by size:
2464 1337 248
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 376 600 2038 2165 376
Size: 224 1438 127 2260
Fragments arranged by size:
2260 1438 224 127
MspA1I CmG'CkG
Cuts at: 115 285 1160 1474 1834 2847 3092 115
Size: 170 875 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 875 360 314 245 170
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 143 1480 1728 143
Size: 1337 248 2464
Fragments arranged by size:
2464 1337 248
NcoI C'CATG_G
Cuts at: 107 744 107
Size: 637 3412
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PfoI T'CCnGG_A
Cuts at: 501 501
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PmlI CAC'GTG
Cuts at: 156 156
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PsiI TTA'TAA
Cuts at: 331 331
Size: 4049
PstI C_TGCA'G
Cuts at: 951 1096 1781 951
Size: 145 685 3219
Fragments arranged by size:
3219 685 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 285 1474 1834 285
Size: 1189 360 2500
Fragments arranged by size:
2500 1189 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 277 872 947 1092 1777 2770 2961 33
Size: 244 595 75 145 685 993 191 1121
Fragments arranged by size:
1121 993 685 595 244 191 145 75
SfoI GGC'GCC
Cuts at: 144 1481 1729 144
Size: 1337 248 2464
Fragments arranged by size:
2464 1337 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 670 1071 670
Size: 401 3648
SmlI C'TyrA_G
Cuts at: 508 1204 2119 2611 2873 508
Size: 696 915 492 262 1684
Fragments arranged by size:
1684 915 696 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
SspI AAT'ATT
Cuts at: 175 571 175
Size: 396 3653
StyI C'CwwG_G
Cuts at: 107 744 1183 1537 107
Size: 637 439 354 2619
Fragments arranged by size:
2619 637 439 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 1765 2224 2433 1632
Size: 133 459 209 3248
Fragments arranged by size:
3248 459 209 133
TatI w'GTAC_w
Cuts at: 578 3564 578
Size: 2986 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 668 1069 668
Size: 401 3648
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI