Restriction map for
pF1KE9630
(Circular) (Six-base) MAPSORT of: pF1KE9630.seq Check: 2369 from: 1 to: 6368
pF1KE9630 6368 bp
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2146 2146
Size: 6368
AccI GT'mk_AC
Cuts at: 1930 3406 1930
Size: 1476 4892
Acc65I G'GTAC_C
Cuts at: 3384 3384
Size: 6368
AclI AA'CG_TT
Cuts at: 6208 6208
Size: 6368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 457 686 1844 3173 3289 4193 4625 5372
Size: 342 229 1158 1329 116 904 432 747
Cuts at: 5372 5640 115
Size: 268 843
Fragments arranged by size:
1329 1158 904 843 747 432 342 268
229 116
AflIII A'CryG_T
Cuts at: 2114 2648 3720 4824 5801 2114
Size: 534 1072 1104 977 2681
Fragments arranged by size:
2681 1104 1072 977 534
AgeI A'CCGG_T
Cuts at: 4756 4756
Size: 6368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 479 479
Size: 6368
AlwNI CAG_nnn'CTG
Cuts at: 1110 2215 3673 5240 1110
Size: 1105 1458 1567 2238
Fragments arranged by size:
2238 1567 1458 1105
ApaI G_GGCC'C
Cuts at: 3312 3312
Size: 6368
ApaLI G'TGCA_C
Cuts at: 769 1492 1963 5138 769
Size: 723 471 3175 1999
Fragments arranged by size:
3175 1999 723 471
ApoI r'AATT_y
Cuts at: 216 271 328 482 850 1348 2065 2443 2557
Size: 55 57 154 368 498 717 378 114
Cuts at: 2557 2596 3196 3372 5721 5977 216
Size: 39 600 176 2349 256 607
Fragments arranged by size:
2349 717 607 600 498 378 368 256
176 154 114 57 55 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 6368
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6368
AvaI C'yCGr_G
Cuts at: 3388 3388
Size: 6368
BamHI G'GATC_C
Cuts at: 3393 3393
Size: 6368
BanI G'GyrC_C
Cuts at: 1179 1878 2046 3384 3798 4046 4081 1179
Size: 699 168 1338 414 248 35 3466
Fragments arranged by size:
3466 1338 699 414 248 168 35
BanII G_rGCy'C
Cuts at: 876 1897 2261 2711 3312 3382 4412 876
Size: 1021 364 450 601 70 1030 2832
Fragments arranged by size:
2832 1030 1021 601 450 364 70
BbeI G_GCGC'C
Cuts at: 3802 4050 3802
Size: 248 6120
BbsI GAAGACnn'nnnn_
Cuts at: 926 1117 926
Size: 191 6177
BciVI GTATCCnnnnn_n'
Cuts at: 4266 5027 6368 4266
Size: 761 1341 4266
Fragments arranged by size:
4266 1341 761
BclI T'GATC_A
Cuts at: 740 3888 740
Size: 3148 3220
BfrBI ATG'CAT
Cuts at: 1603 1603
Size: 6368
BglII A'GATC_T
Cuts at: 2529 3883 5920 2529
Size: 1354 2037 2977
Fragments arranged by size:
2977 2037 1354
BlpI GC'TnA_GC
Cuts at: 3037 3480 3037
Size: 443 5925
Bme1580I G_kGCm'C
Cuts at: 773 1496 1967 3312 3993 4086 5142 773
Size: 723 471 1345 681 93 1056 1999
Fragments arranged by size:
1999 1345 1056 723 681 471 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1608 1877 2607 3580 3661 3996 6140 1453
Size: 155 269 730 973 81 335 2144 1681
Fragments arranged by size:
2144 1681 973 730 335 269 155 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1271 4735 1271
Size: 3464 2904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 710
Size: 6368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 2762 3544 4423 4915 5213 554
Size: 2208 782 879 492 298 1709
Fragments arranged by size:
2208 1709 879 782 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6072 6072
Size: 6368
BsaAI yAC'GTr
Cuts at: 1078 3316 3721 1078
Size: 2238 405 3725
Fragments arranged by size:
3725 2238 405
BsaBI GATnn'nnATC
Cuts at: 6 3230 3882 5919 6
Size: 3224 652 2037 455
Fragments arranged by size:
3224 2037 652 455
BsaHI Gr'CG_yC
Cuts at: 3799 4047 4749 6245 3799
Size: 248 702 1496 3922
Fragments arranged by size:
3922 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3557 4078 4756 5030 5177 5588 3557
Size: 521 678 274 147 411 4337
Fragments arranged by size:
4337 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1247 3152 1247
Size: 1905 4463
BseYI C'CCAG_C
Cuts at: 1100 1233 2254 3793 4551 5128 5624 1100
Size: 133 1021 1539 758 577 496 1844
Fragments arranged by size:
1844 1539 1021 758 577 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 1901 1495
Size: 406 5962
BsiEI CG_ry'CG
Cuts at: 104 3910 3956 5164 104
Size: 3806 46 1208 1308
Fragments arranged by size:
3806 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 773 1496 1967 2711 3382 4160 4350 5142 773
Size: 723 471 744 671 778 190 792 1999
Fragments arranged by size:
1999 792 778 744 723 671 471 190
BsmI GAATG_Cn'
Cuts at: 2813 2813
Size: 6368
BsmBI CGTCTCn'nnnn_
Cuts at: 2114 2114
Size: 6368
Bsp1286I G_dGCh'C
Cuts at: 773 876 1496 1897 1967 2261 2711 3312 3382
Size: 103 620 401 70 294 450 601 70
Cuts at: 3382 3993 4086 4160 4350 4412 5142 773
Size: 611 93 74 190 62 730 1999
Fragments arranged by size:
1999 730 620 611 601 450 401 294
190 103 93 74 70 70 62
BspEI T'CCGG_A
Cuts at: 5588 5588
Size: 6368
BspHI T'CATG_A
Cuts at: 1344 1520 5544 1344
Size: 176 4024 2168
Fragments arranged by size:
4024 2168 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 795 2146 3418 3632 3934 4315 795
Size: 1351 1272 214 302 381 2848
Fragments arranged by size:
2848 1351 1272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4660 4714 6198 6363 50
Size: 4610 54 1484 165 55
Fragments arranged by size:
4610 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 174 413 913 1250 2225 2416 4280 174
Size: 239 500 337 975 191 1864 2262
Fragments arranged by size:
2262 1864 975 500 337 239 191
BsrFI r'CCGG_y
Cuts at: 4366 4547 4756 4366
Size: 181 209 5978
Fragments arranged by size:
5978 209 181
BsrGI T'GTAC_A
Cuts at: 374 410 374
Size: 36 6332
BssSI C'ACGA_G
Cuts at: 4639 4997 4639
Size: 358 6010
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 3745 1924
Size: 1821 4547
BstBI TT'CG_AA
Cuts at: 4729 4729
Size: 6368
BstXI CCAn_nnnn'nTGG
Cuts at: 388 388
Size: 6368
BstYI r'GATC_y
Cuts at: 1 1035 2235 2529 3393 3860 3883 4218 4464
Size: 1034 1200 294 864 467 23 335 246
Cuts at: 4464 5476 5562 5574 5920 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1200 1034 1012 864 467 449 346 335
294 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 381 2329 3049 107
Size: 274 1948 720 3426
Fragments arranged by size:
3426 1948 720 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4318 4499 4662 4318
Size: 181 163 6024
Fragments arranged by size:
6024 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 2697 3611 1310
Size: 1387 914 4067
Fragments arranged by size:
4067 1387 914
ClaI AT'CG_AT
Cuts at: 3065 3065
Size: 6368
DraI TTT'AAA
Cuts at: 326 611 2567 3367 326
Size: 285 1956 800 3327
Fragments arranged by size:
3327 1956 800 285
DraIII CAC_nnn'GTG
Cuts at: 1363 1363
Size: 6368
DrdI GACnn_nn'nnGTC
Cuts at: 4074 4932 4074
Size: 858 5510
EaeI y'GGCC_r
Cuts at: 1307 3953 4127 4518 4545 5890 1307
Size: 2646 174 391 27 1345 1785
Fragments arranged by size:
2646 1785 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3953 3953
Size: 6368
EarI CTCTTCn'nnn_
Cuts at: 206 729 1674 1820 4391 4601 206
Size: 523 945 146 2571 210 1973
Fragments arranged by size:
2571 1973 945 523 210 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4576 4886 5032 6178 4576
Size: 310 146 1146 4766
Fragments arranged by size:
4766 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2709 3380 2709
Size: 671 5697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 457 686 710 1844 3173 3289 4193 4625
Size: 342 229 24 1134 1329 116 904 432
Cuts at: 4625 5372 5640 115
Size: 747 268 843
Fragments arranged by size:
1329 1134 904 843 747 432 342 268
229 116 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 6368
EcoO109I rG'GnC_Cy
Cuts at: 1760 2609 3308 3309 3507 1760
Size: 849 699 1 198 4621
Fragments arranged by size:
4621 849 699 198 1
EcoRI G'AATT_C
Cuts at: 482 1348 2596 3372 482
Size: 866 1248 776 3478
Fragments arranged by size:
3478 1248 866 776
EcoRV GAT'ATC
Cuts at: 698 698
Size: 6368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4387 4387
Size: 6368
FspI TGC'GCA
Cuts at: 4149 4149
Size: 6368
HaeII r_GCGC'y
Cuts at: 1120 3802 4050 5072 6051 1120
Size: 2682 248 1022 979 1437
Fragments arranged by size:
2682 1437 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1358 1390 1894 1926 2373 2405 2905
Size: 32 623 32 504 32 447 32 500
Cuts at: 2905 2937 4210 4240 4242 4272 703
Size: 32 1273 30 2 30 2799
Fragments arranged by size:
2799 1273 623 504 500 447 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3407 3407
Size: 6368
HindIII A'AGCT_T
Cuts at: 829 1945 829
Size: 1116 5252
KasI G'GCGC_C
Cuts at: 3798 4046 3798
Size: 248 6120
KpnI G_GTAC'C
Cuts at: 3388 3388
Size: 6368
MfeI C'AATT_G
Cuts at: 2449 2449
Size: 6368
MluI A'CGCG_T
Cuts at: 5801 5801
Size: 6368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5039 5223 5039
Size: 184 6184
MscI TGG'CCA
Cuts at: 1309 4129 1309
Size: 2820 3548
MslI CAynn'nnrTG
Cuts at: 461 1525 2044 2653 2837 3115 3121 4357 4484
Size: 1064 519 609 184 278 6 1236 127
Cuts at: 4484 461
Size: 2345
Fragments arranged by size:
2345 1236 1064 609 519 278 184 127
6
MspA1I CmG'CkG
Cuts at: 3479 3793 4153 5166 5411 3479
Size: 314 360 1013 245 4436
Fragments arranged by size:
4436 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4549 4549
Size: 6368
NarI GG'CG_CC
Cuts at: 3799 4047 3799
Size: 248 6120
NcoI C'CATG_G
Cuts at: 107 381 2329 3049 107
Size: 274 1948 720 3426
Fragments arranged by size:
3426 1948 720 274
NgoMIV G'CCGG_C
Cuts at: 4547 4547
Size: 6368
NsiI A_TGCA'T
Cuts at: 1605 1605
Size: 6368
NspI r_CATG'y
Cuts at: 153 460 2118 2652 3102 3421 3921 4828 153
Size: 307 1658 534 450 319 500 907 1693
Fragments arranged by size:
1693 1658 907 534 500 450 319 307
PasI CC'CwG_GG
Cuts at: 2523 2591 2523
Size: 68 6300
PciI A'CATG_T
Cuts at: 2114 2648 4824 2114
Size: 534 2176 3658
Fragments arranged by size:
3658 2176 534
PflMI CCAn_nnn'nTGG
Cuts at: 1363 2591 2619 1363
Size: 1228 28 5112
Fragments arranged by size:
5112 1228 28
PfoI T'CCnGG_A
Cuts at: 639 639
Size: 6368
PmeI GTTT'AAAC
Cuts at: 3367 3367
Size: 6368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 5507
Size: 32 6336
PpuMI rG'GwC_Cy
Cuts at: 1760 2609 1760
Size: 849 5519
PshAI GACnn'nnGTC
Cuts at: 2948 2948
Size: 6368
PspOMI G'GGCC_C
Cuts at: 3308 3308
Size: 6368
PstI C_TGCA'G
Cuts at: 455 1221 2157 3415 4100 455
Size: 766 936 1258 685 2723
Fragments arranged by size:
2723 1258 936 766 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6368
PvuII CAG'CTG
Cuts at: 3793 4153 3793
Size: 360 6008
RsrII CG'GwC_CG
Cuts at: 4563 4563
Size: 6368
SacI G_AGCT'C
Cuts at: 2711 3382 2711
Size: 671 5697
SalI G'TCGA_C
Cuts at: 3405 3405
Size: 6368
SanDI GG'GwC_CC
Cuts at: 2609 2609
Size: 6368
SapI GCTCTTCn'nnn_
Cuts at: 4391 4601 4391
Size: 210 6158
SbfI CC_TGCA'GG
Cuts at: 3415 3415
Size: 6368
ScaI AGT'ACT
Cuts at: 1341 5885 1341
Size: 4544 1824
SfcI C'TryA_G
Cuts at: 33 451 1217 2153 2712 3411 4096 5089 5280
Size: 418 766 936 559 699 685 993 191
Cuts at: 5280 33
Size: 1121
Fragments arranged by size:
1121 993 936 766 699 685 559 418
191
SfoI GGC'GCC
Cuts at: 3800 4048 3800
Size: 248 6120
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6368
SmaI CCC'GGG
Cuts at: 3390 3390
Size: 6368
SmlI C'TyrA_G
Cuts at: 569 2777 3523 4438 4930 5192 569
Size: 2208 746 915 492 262 1745
Fragments arranged by size:
2208 1745 915 746 492 262
SnaBI TAC'GTA
Cuts at: 3316 3316
Size: 6368
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6368
SphI G_CATG'C
Cuts at: 460 3102 3421 3921 460
Size: 2642 319 500 2907
Fragments arranged by size:
2907 2642 500 319
SspI AAT'ATT
Cuts at: 763 3219 763
Size: 2456 3912
StuI AGG'CCT
Cuts at: 2101 2101
Size: 6368
StyI C'CwwG_G
Cuts at: 107 381 441 2329 3049 3502 3856 107
Size: 274 60 1888 720 453 354 2619
Fragments arranged by size:
2619 1888 720 453 354 274 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 1896 3951 4084 4543 4752 1500
Size: 396 2055 133 459 209 3116
Fragments arranged by size:
3116 2055 459 396 209 133
TatI w'GTAC_w
Cuts at: 338 374 410 1339 3330 5883 338
Size: 36 36 929 1991 2553 823
Fragments arranged by size:
2553 1991 929 823 36 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5885 5917 5885
Size: 32 6336
Tth111I GACn'n_nGTC
Cuts at: 4165 4165
Size: 6368
XbaI T'CTAG_A
Cuts at: 1584 3399 1584
Size: 1815 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1302 2482 3863 1302
Size: 1180 1381 3807
Fragments arranged by size:
3807 1381 1180
XmaI C'CCGG_G
Cuts at: 3388 3388
Size: 6368
XmnI GAAnn'nnTTC
Cuts at: 2846 3200 2846
Size: 354 6014
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BssHII BstEII BstZ17I Bsu36I CspCI FseI FspAI
HpaI NdeI NheI NotI NruI PacI PmlI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SwaI
XhoI ZraI