Restriction map for
pF1KE9635
(Circular) (Six-base) MAPSORT of: pF1KE9635.seq Check: 2086 from: 1 to: 7745
pF1KE9635 7745 bp
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AatII G_ACGT'C
Cuts at: 882 2928 882
Size: 2046 5699
AccI GT'mk_AC
Cuts at: 4783 4783
Size: 7745
Acc65I G'GTAC_C
Cuts at: 3723 4761 3723
Size: 1038 6707
AclI AA'CG_TT
Cuts at: 7585 7585
Size: 7745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3154 3509 3808 4357 5570 6002 6749 7017 3154
Size: 355 299 549 1213 432 747 268 3882
Fragments arranged by size:
3882 1213 747 549 432 355 299 268
AflIII A'CryG_T
Cuts at: 471 3201 5097 6201 7178 471
Size: 2730 1896 1104 977 1038
Fragments arranged by size:
2730 1896 1104 1038 977
AgeI A'CCGG_T
Cuts at: 6133 6133
Size: 7745
AleI CACnn'nnGTG
Cuts at: 198 1646 198
Size: 1448 6297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 561 561
Size: 7745
AlwNI CAG_nnn'CTG
Cuts at: 375 2997 3164 3744 3912 5050 6617 375
Size: 2622 167 580 168 1138 1567 1503
Fragments arranged by size:
2622 1567 1503 1138 580 168 167
ApaI G_GGCC'C
Cuts at: 166 172 166
Size: 6 7739
ApaLI G'TGCA_C
Cuts at: 6515 6515
Size: 7745
ApoI r'AATT_y
Cuts at: 411 464 638 1333 1405 1485 2476 2803 3267
Size: 53 174 695 72 80 991 327 464
Cuts at: 3267 3348 3800 3934 4193 4749 7098 7354 411
Size: 81 452 134 259 556 2349 256 802
Fragments arranged by size:
2349 991 802 695 556 464 452 327
259 256 174 134 81 80 72 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 7745
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7745
AvaI C'yCGr_G
Cuts at: 157 165 196 841 4765 157
Size: 8 31 645 3924 3137
Fragments arranged by size:
3924 3137 645 31 8
AvrII C'CTAG_G
Cuts at: 2937 2937
Size: 7745
BamHI G'GATC_C
Cuts at: 4770 4770
Size: 7745
BanI G'GyrC_C
Cuts at: 3723 4761 5175 5423 5458 3723
Size: 1038 414 248 35 6010
Fragments arranged by size:
6010 1038 414 248 35
BanII G_rGCy'C
Cuts at: 166 172 613 2633 3440 3906 3958 4759 5789
Size: 6 441 2020 807 466 52 801 1030
Cuts at: 5789 166
Size: 2122
Fragments arranged by size:
2122 2020 1030 807 801 466 441 52
6
BbeI G_GCGC'C
Cuts at: 5179 5427 5179
Size: 248 7497
BbsI GAAGACnn'nnnn_
Cuts at: 201 1076 1616 4547 4697 201
Size: 875 540 2931 150 3249
Fragments arranged by size:
3249 2931 875 540 150
BbvCI CC'TCA_GC
Cuts at: 1898 3299 3987 1898
Size: 1401 688 5656
Fragments arranged by size:
5656 1401 688
BciVI GTATCCnnnnn_n'
Cuts at: 376 2851 3841 4126 4191 5643 6404 7745 376
Size: 2475 990 285 65 1452 761 1341 376
Fragments arranged by size:
2475 1452 1341 990 761 376 285 65
BclI T'GATC_A
Cuts at: 5265 5265
Size: 7745
BfrBI ATG'CAT
Cuts at: 2347 2347
Size: 7745
BglI GCCn_nnn'nGGC
Cuts at: 112 176 315 112
Size: 64 139 7542
Fragments arranged by size:
7542 139 64
BglII A'GATC_T
Cuts at: 485 5260 7297 485
Size: 4775 2037 933
Fragments arranged by size:
4775 2037 933
BlpI GC'TnA_GC
Cuts at: 1688 4238 4857 1688
Size: 2550 619 4576
Fragments arranged by size:
4576 2550 619
Bme1580I G_kGCm'C
Cuts at: 166 172 205 784 5370 5463 6519 166
Size: 6 33 579 4586 93 1056 1392
Fragments arranged by size:
4586 1392 1056 579 93 33 6
BmgBI CAC'GTC
Cuts at: 3202 3202
Size: 7745
BmrI ACTGGGnnnn_n'
Cuts at: 447 1653 3027 3722 4071 4620 4957 5038 5373
Size: 1206 1374 695 349 549 337 81 335
Cuts at: 5373 7517 447
Size: 2144 675
Fragments arranged by size:
2144 1374 1206 695 675 549 349 337
335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4378 4380 6112 4378
Size: 2 1732 6011
Fragments arranged by size:
6011 1732 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1638 1706 3215 3511 4159 4350 1179
Size: 459 68 1509 296 648 191 4574
Fragments arranged by size:
4574 1509 648 459 296 191 68
Bpu10I CC'TnA_GC
Cuts at: 1181 1898 3299 3957 3987 1181
Size: 717 1401 658 30 4939
Fragments arranged by size:
4939 1401 717 658 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3028 4345 4921 5800 6292 6590 3028
Size: 1317 576 879 492 298 4183
Fragments arranged by size:
4183 1317 879 576 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4260 7449 4260
Size: 3189 4556
BsaAI yAC'GTr
Cuts at: 364 5098 364
Size: 4734 3011
BsaBI GATnn'nnATC
Cuts at: 6 2586 2839 5259 7296 6
Size: 2580 253 2420 2037 455
Fragments arranged by size:
2580 2420 2037 455 253
BsaHI Gr'CG_yC
Cuts at: 879 2925 4344 5176 5424 6126 7622 879
Size: 2046 1419 832 248 702 1496 1002
Fragments arranged by size:
2046 1496 1419 1002 832 702 248
BsaWI w'CCGG_w
Cuts at: 667 4934 5455 6133 6407 6554 6965 667
Size: 4267 521 678 274 147 411 1447
Fragments arranged by size:
4267 1447 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2884 2914 3188 3218 4589 4619 2884
Size: 30 274 30 1371 30 6010
Fragments arranged by size:
6010 1371 274 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 1375 1477 1741 3031 859
Size: 516 102 264 1290 5573
Fragments arranged by size:
5573 1290 516 264 102
BseYI C'CCAG_C
Cuts at: 174 217 221 786 1739 1831 2896 3660 3905
Size: 43 4 565 953 92 1065 764 245
Cuts at: 3905 4166 4270 4569 5170 5928 6505 7001 174
Size: 261 104 299 601 758 577 496 918
Fragments arranged by size:
1065 953 918 764 758 601 577 565
496 299 261 245 104 92 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 784 1899 2896 3169 112
Size: 672 1115 997 273 4688
Fragments arranged by size:
4688 1115 997 672 273
BsiEI CG_ry'CG
Cuts at: 104 304 5287 5333 6541 104
Size: 200 4983 46 1208 1308
Fragments arranged by size:
4983 1308 1208 200 46
BsiHKAI G_wGCw'C
Cuts at: 2633 3017 4759 5537 5727 6519 2633
Size: 384 1742 778 190 792 3859
Fragments arranged by size:
3859 1742 792 778 384 190
BsmI GAATG_Cn'
Cuts at: 2349 2349
Size: 7745
BsmBI CGTCTCn'nnnn_
Cuts at: 870 2916 2932 4335 870
Size: 2046 16 1403 4280
Fragments arranged by size:
4280 2046 1403 16
Bsp1286I G_dGCh'C
Cuts at: 166 172 205 613 784 2633 3017 3440 3906
Size: 6 33 408 171 1849 384 423 466
Cuts at: 3906 3958 4759 5370 5463 5537 5727 5789 6519
Size: 52 801 611 93 74 190 62 730
Cuts at: 6519 166
Size: 1392
Fragments arranged by size:
1849 1392 801 730 611 466 423 408
384 190 171 93 74 62 52 33
6
BspEI T'CCGG_A
Cuts at: 667 6965 667
Size: 6298 1447
BspHI T'CATG_A
Cuts at: 1772 1797 6921 1772
Size: 25 5124 2596
Fragments arranged by size:
5124 2596 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4795 5009 5311 5692 4795
Size: 214 302 381 6848
Fragments arranged by size:
6848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4374 6037 6091 7575 7740 50
Size: 4324 1663 54 1484 165 55
Fragments arranged by size:
4324 1663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1068 1391 5657 1068
Size: 323 4266 3156
Fragments arranged by size:
4266 3156 323
BsrFI r'CCGG_y
Cuts at: 1073 5743 5924 6133 1073
Size: 4670 181 209 2685
Fragments arranged by size:
4670 2685 209 181
BssSI C'ACGA_G
Cuts at: 398 3239 6016 6374 398
Size: 2841 2777 358 1769
Fragments arranged by size:
2841 2777 1769 358
BstAPI GCAn_nnn'nTGC
Cuts at: 762 2556 2784 2997 5122 762
Size: 1794 228 213 2125 3385
Fragments arranged by size:
3385 2125 1794 228 213
BstBI TT'CG_AA
Cuts at: 212 6106 212
Size: 5894 1851
BstXI CCAn_nnnn'nTGG
Cuts at: 1026 1661 3717 4624 1026
Size: 635 2056 907 4147
Fragments arranged by size:
4147 2056 907 635
BstYI r'GATC_y
Cuts at: 1 260 485 1746 2697 3104 4770 5237 5260
Size: 259 225 1261 951 407 1666 467 23
Cuts at: 5260 5595 5841 6853 6939 6951 7297 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1666 1261 1012 951 467 449 407 346
335 259 246 225 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 679 1454 3556 3848 679
Size: 775 2102 292 4576
Fragments arranged by size:
4576 2102 775 292
BtgI C'CryG_G
Cuts at: 107 4125 107
Size: 4018 3727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5695 5876 6039 5695
Size: 181 163 7401
Fragments arranged by size:
7401 181 163
BtsI GCAGTG_nn'
Cuts at: 124 611 1062 1419 2972 4281 4988 124
Size: 487 451 357 1553 1309 707 2881
Fragments arranged by size:
2881 1553 1309 707 487 451 357
ClaI AT'CG_AT
Cuts at: 1974 2586 1974
Size: 612 7133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1302 1337 3694 3729 1302
Size: 35 2357 35 5318
Fragments arranged by size:
5318 2357 35 35
DraI TTT'AAA
Cuts at: 4744 4744
Size: 7745
DrdI GACnn_nn'nnGTC
Cuts at: 1036 5451 6309 1036
Size: 4415 858 2472
Fragments arranged by size:
4415 2472 858
EaeI y'GGCC_r
Cuts at: 116 301 2072 5330 5504 5895 5922 7267 116
Size: 185 1771 3258 174 391 27 1345 594
Fragments arranged by size:
3258 1771 1345 594 391 185 174 27
EagI C'GGCC_G
Cuts at: 301 5330 301
Size: 5029 2716
EarI CTCTTCn'nnn_
Cuts at: 221 922 1454 2666 2864 3095 4333 5768 5978
Size: 701 532 1212 198 231 1238 1435 210
Cuts at: 5978 221
Size: 1988
Fragments arranged by size:
1988 1435 1238 1212 701 532 231 210
198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5953 6263 6409 7555 5953
Size: 310 146 1146 6143
Fragments arranged by size:
6143 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2631 4757 2631
Size: 2126 5619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1638 1706 3154 3215 3509 3511 3808 4159
Size: 459 68 1448 61 294 2 297 351
Cuts at: 4159 4350 4357 5570 6002 6749 7017 1179
Size: 191 7 1213 432 747 268 1907
Fragments arranged by size:
1907 1448 1213 747 459 432 351 297
294 268 191 68 61 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3847 3847
Size: 7745
EcoO109I rG'GnC_Cy
Cuts at: 162 3183 3657 3741 4359 4884 162
Size: 3021 474 84 618 525 3023
Fragments arranged by size:
3023 3021 618 525 474 84
EcoRI G'AATT_C
Cuts at: 638 3267 3348 4749 638
Size: 2629 81 1401 3634
Fragments arranged by size:
3634 2629 1401 81
EcoRV GAT'ATC
Cuts at: 2584 2584
Size: 7745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2718 3456 5764 2718
Size: 738 2308 4699
Fragments arranged by size:
4699 2308 738
FspI TGC'GCA
Cuts at: 2549 5526 2549
Size: 2977 4768
HaeII r_GCGC'y
Cuts at: 151 5179 5427 6449 7428 151
Size: 5028 248 1022 979 468
Fragments arranged by size:
5028 1022 979 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1116 1148 1381 1413 2096 2128 2884 2916 4240
Size: 32 233 32 683 32 756 32 1324
Cuts at: 4240 4272 5587 5617 5619 5649 1116
Size: 32 1315 30 2 30 3212
Fragments arranged by size:
3212 1324 1315 756 683 233 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1908 2618 4784 1908
Size: 710 2166 4869
Fragments arranged by size:
4869 2166 710
HindIII A'AGCT_T
Cuts at: 2096 2353 2399 2882 2096
Size: 257 46 483 6959
Fragments arranged by size:
6959 483 257 46
HpaI GTT'AAC
Cuts at: 1908 1908
Size: 7745
KasI G'GCGC_C
Cuts at: 5175 5423 5175
Size: 248 7497
KpnI G_GTAC'C
Cuts at: 3727 4765 3727
Size: 1038 6707
MluI A'CGCG_T
Cuts at: 7178 7178
Size: 7745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 919 3751 6416 6600 919
Size: 2832 2665 184 2064
Fragments arranged by size:
2832 2665 2064 184
MscI TGG'CCA
Cuts at: 2074 5506 2074
Size: 3432 4313
MslI CAynn'nnrTG
Cuts at: 198 330 1501 1646 4702 5734 5861 198
Size: 132 1171 145 3056 1032 127 2082
Fragments arranged by size:
3056 2082 1171 1032 145 132 127
MspA1I CmG'CkG
Cuts at: 174 221 2652 3664 4170 4573 4631 4856 5170
Size: 47 2431 1012 506 403 58 225 314
Cuts at: 5170 5530 6543 6788 174
Size: 360 1013 245 1131
Fragments arranged by size:
2431 1131 1013 1012 506 403 360 314
245 225 58 47
NaeI GCC'GGC
Cuts at: 5926 5926
Size: 7745
NarI GG'CG_CC
Cuts at: 5176 5424 5176
Size: 248 7497
NcoI C'CATG_G
Cuts at: 107 4125 107
Size: 4018 3727
NdeI CA'TA_TG
Cuts at: 1304 1304
Size: 7745
NgoMIV G'CCGG_C
Cuts at: 5924 5924
Size: 7745
NotI GC'GGCC_GC
Cuts at: 301 301
Size: 7745
NsiI A_TGCA'T
Cuts at: 2349 2349
Size: 7745
NspI r_CATG'y
Cuts at: 335 475 1296 1500 2204 4798 5298 6205 335
Size: 140 821 204 704 2594 500 907 1875
Fragments arranged by size:
2594 1875 907 821 704 500 204 140
PasI CC'CwG_GG
Cuts at: 1675 1675
Size: 7745
PciI A'CATG_T
Cuts at: 471 6201 471
Size: 5730 2015
PflMI CCAn_nnn'nTGG
Cuts at: 3164 3627 3164
Size: 463 7282
PmeI GTTT'AAAC
Cuts at: 4744 4744
Size: 7745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 626 658 6884 6916 626
Size: 32 6226 32 1455
Fragments arranged by size:
6226 1455 32 32
PshAI GACnn'nnGTC
Cuts at: 3999 3999
Size: 7745
PsiI TTA'TAA
Cuts at: 2291 2291
Size: 7745
PspOMI G'GGCC_C
Cuts at: 162 168 162
Size: 6 7739
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 7713
PstI C_TGCA'G
Cuts at: 2780 2993 3191 3694 4589 4792 5477 2780
Size: 213 198 503 895 203 685 5048
Fragments arranged by size:
5048 895 685 503 213 203 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7745
PvuII CAG'CTG
Cuts at: 221 2652 3664 4170 4573 4631 5170 5530 221
Size: 2431 1012 506 403 58 539 360 2436
Fragments arranged by size:
2436 2431 1012 539 506 403 360 58
RsrII CG'GwC_CG
Cuts at: 5940 5940
Size: 7745
SacI G_AGCT'C
Cuts at: 2633 4759 2633
Size: 2126 5619
SalI G'TCGA_C
Cuts at: 4782 4782
Size: 7745
SapI GCTCTTCn'nnn_
Cuts at: 2666 5768 5978 2666
Size: 3102 210 4433
Fragments arranged by size:
4433 3102 210
SbfI CC_TGCA'GG
Cuts at: 3191 4792 3191
Size: 1601 6144
ScaI AGT'ACT
Cuts at: 436 456 7262 436
Size: 20 6806 919
Fragments arranged by size:
6806 919 20
SexAI A'CCwGG_T
Cuts at: 1660 1660
Size: 7745
SfcI C'TryA_G
Cuts at: 33 2776 2989 3187 3690 4585 4788 5473 6466
Size: 2743 213 198 503 895 203 685 993
Cuts at: 6466 6657 33
Size: 191 1121
Fragments arranged by size:
2743 1121 993 895 685 503 213 203
198 191
SfoI GGC'GCC
Cuts at: 5177 5425 5177
Size: 248 7497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7745
SmaI CCC'GGG
Cuts at: 167 4767 167
Size: 4600 3145
SmlI C'TyrA_G
Cuts at: 3043 4324 4900 5815 6307 6569 3043
Size: 1281 576 915 492 262 4219
Fragments arranged by size:
4219 1281 915 576 492 262
SpeI A'CTAG_T
Cuts at: 67 1344 67
Size: 1277 6468
SphI G_CATG'C
Cuts at: 4798 5298 4798
Size: 500 7245
SrfI GCCC'GGGC
Cuts at: 167 167
Size: 7745
SspI AAT'ATT
Cuts at: 828 828
Size: 7745
StyI C'CwwG_G
Cuts at: 107 1148 1561 2021 2937 4125 4436 4879 5233
Size: 1041 413 460 916 1188 311 443 354
Cuts at: 5233 107
Size: 2619
Fragments arranged by size:
2619 1188 1041 916 460 443 413 354
311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 146 4265 5328 5461 5920 6129 146
Size: 4119 1063 133 459 209 1762
Fragments arranged by size:
4119 1762 1063 459 209 133
TatI w'GTAC_w
Cuts at: 434 454 728 4711 7260 434
Size: 20 274 3983 2549 919
Fragments arranged by size:
3983 2549 919 274 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3306 3338 4589 4621 7262 7294 3188
Size: 32 86 32 1251 32 2641 32 3639
Fragments arranged by size:
3639 2641 1251 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4612 5542 4612
Size: 930 6815
XbaI T'CTAG_A
Cuts at: 4776 4776
Size: 7745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1023 5240 1023
Size: 4217 3528
XmaI C'CCGG_G
Cuts at: 165 4765 165
Size: 4600 3145
XmnI GAAnn'nnTTC
Cuts at: 638 2476 2720 4450 4484 638
Size: 1838 244 1730 34 3899
Fragments arranged by size:
3899 1838 1730 244 34
ZraI GAC'GTC
Cuts at: 880 2926 880
Size: 2046 5699
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI BaeI BcgI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I DraIII FseI
FspAI MfeI NheI NruI PacI PfoI PmlI PpuMI
PspXI SacII SanDI SfiI SgrAI SnaBI StuI SwaI
XhoI