Restriction map for
pF1KE9636
(Circular) (Six-base) MAPSORT of: pF1KE9636.seq Check: 1223 from: 1 to: 7790
pF1KE9636 7790 bp
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2865 3158 3669 2865
Size: 293 511 6986
Fragments arranged by size:
6986 511 293
AccI GT'mk_AC
Cuts at: 2142 2356 2640 4828 2142
Size: 214 284 2188 5104
Fragments arranged by size:
5104 2188 284 214
Acc65I G'GTAC_C
Cuts at: 4806 4806
Size: 7790
AclI AA'CG_TT
Cuts at: 7630 7630
Size: 7790
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 2413 2522 4019 4688 5615 6047 6794 7062
Size: 2134 109 1497 669 927 432 747 268
Cuts at: 7062 279
Size: 1007
Fragments arranged by size:
2134 1497 1007 927 747 669 432 268
109
AflIII A'CryG_T
Cuts at: 637 2242 5142 6246 7223 637
Size: 1605 2900 1104 977 1204
Fragments arranged by size:
2900 1605 1204 1104 977
AgeI A'CCGG_T
Cuts at: 6178 6178
Size: 7790
AhdI GACnn_n'nnGTC
Cuts at: 2965 2965
Size: 7790
AleI CACnn'nnGTG
Cuts at: 144 916 1688 144
Size: 772 772 6246
Fragments arranged by size:
6246 772 772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1615 2592 4150 1615
Size: 977 1558 5255
Fragments arranged by size:
5255 1558 977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1360 1392 1360
Size: 32 7758
AlwNI CAG_nnn'CTG
Cuts at: 321 2179 2343 2814 3030 3330 3538 3684 3906
Size: 1858 164 471 216 300 208 146 222
Cuts at: 3906 3990 4096 4371 4570 5095 6662 321
Size: 84 106 275 199 525 1567 1449
Fragments arranged by size:
1858 1567 1449 525 471 300 275 222
216 208 199 164 146 106 84
ApaI G_GGCC'C
Cuts at: 4296 4467 4624 4296
Size: 171 157 7462
Fragments arranged by size:
7462 171 157
ApaLI G'TGCA_C
Cuts at: 6560 6560
Size: 7790
ApoI r'AATT_y
Cuts at: 379 1447 3381 3826 4794 7143 7399 379
Size: 1068 1934 445 968 2349 256 770
Fragments arranged by size:
2349 1934 1068 968 770 445 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7790
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7790
AvaI C'yCGr_G
Cuts at: 512 1097 1271 2585 3191 3922 4289 4330 4454
Size: 585 174 1314 606 731 367 41 124
Cuts at: 4454 4523 4810 512
Size: 69 287 3492
Fragments arranged by size:
3492 1314 731 606 585 367 287 174
124 69 41
BamHI G'GATC_C
Cuts at: 4403 4815 4403
Size: 412 7378
BanI G'GyrC_C
Cuts at: 1205 1350 1484 1599 1713 1982 2847 2938 2974
Size: 145 134 115 114 269 865 91 36
Cuts at: 2974 3857 4806 5220 5468 5503 1205
Size: 883 949 414 248 35 3492
Fragments arranged by size:
3492 949 883 865 414 269 248 145
134 115 114 91 36 35
BanII G_rGCy'C
Cuts at: 400 513 2592 2663 3702 4037 4210 4296 4310
Size: 113 2079 71 1039 335 173 86 14
Cuts at: 4310 4455 4467 4558 4624 4804 5834 400
Size: 145 12 91 66 180 1030 2356
Fragments arranged by size:
2356 2079 1039 1030 335 180 173 145
113 91 86 71 66 14 12
BbeI G_GCGC'C
Cuts at: 1986 3861 5224 5472 1986
Size: 1875 1363 248 4304
Fragments arranged by size:
4304 1875 1363 248
BbsI GAAGACnn'nnnn_
Cuts at: 785 1617 1705 2019 785
Size: 832 88 314 6556
Fragments arranged by size:
6556 832 314 88
BbvCI CC'TCA_GC
Cuts at: 2759 2925 3763 4571 4772 2759
Size: 166 838 808 201 5777
Fragments arranged by size:
5777 838 808 201 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 209 243 3580 3614 209
Size: 34 3337 34 4385
Fragments arranged by size:
4385 3337 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3595 5688 6449 7790 3595
Size: 2093 761 1341 3595
Fragments arranged by size:
3595 2093 1341 761
BclI T'GATC_A
Cuts at: 5310 5310
Size: 7790
BglI GCCn_nnn'nGGC
Cuts at: 1980 2282 2755 3915 3985 1980
Size: 302 473 1160 70 5785
Fragments arranged by size:
5785 1160 473 302 70
BglII A'GATC_T
Cuts at: 815 4221 5305 7342 815
Size: 3406 1084 2037 1263
Fragments arranged by size:
3406 2037 1263 1084
BlpI GC'TnA_GC
Cuts at: 2180 4031 4902 2180
Size: 1851 871 5068
Fragments arranged by size:
5068 1851 871
Bme1580I G_kGCm'C
Cuts at: 151 1210 1355 1604 1718 2493 2941 4296 4467
Size: 1059 145 249 114 775 448 1355 171
Cuts at: 4467 4624 5415 5508 6564 151
Size: 157 791 93 1056 1377
Fragments arranged by size:
1377 1355 1059 1056 791 775 448 249
171 157 145 114 93
BmgBI CAC'GTC
Cuts at: 640 640
Size: 7790
BmrI ACTGGGnnnn_n'
Cuts at: 1056 1695 3614 4619 5002 5083 5418 7562 1056
Size: 639 1919 1005 383 81 335 2144 1284
Fragments arranged by size:
2144 1919 1284 1005 639 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3375 4224 4469 6157 3375
Size: 849 245 1688 5008
Fragments arranged by size:
5008 1688 849 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 1000 1401 1680 2179 2310 2467 2775 2851
Size: 365 401 279 499 131 157 308 76
Cuts at: 2851 3097 3319 3366 3421 3889 4177 4246 4417
Size: 246 222 47 55 468 288 69 171
Cuts at: 4417 4438 4444 4489 4618 635
Size: 21 6 45 129 3807
Fragments arranged by size:
3807 499 468 401 365 308 288 279
246 222 171 157 131 129 76 69
55 47 45 21 6
Bpu10I CC'TnA_GC
Cuts at: 2759 2925 3185 3763 4209 4557 4571 4772 2759
Size: 166 260 578 446 348 14 201 5777
Fragments arranged by size:
5777 578 446 348 260 201 166 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3130 3154 3358 3837 4966 5845 6337 6635 3130
Size: 24 204 479 1129 879 492 298 4285
Fragments arranged by size:
4285 1129 879 492 479 298 204 24
BsaI GGTCTCn'nnnn_
Cuts at: 176 3865 7494 176
Size: 3689 3629 472
Fragments arranged by size:
3689 3629 472
BsaAI yAC'GTr
Cuts at: 1614 2243 5143 1614
Size: 629 2900 4261
Fragments arranged by size:
4261 2900 629
BsaBI GATnn'nnATC
Cuts at: 6 2116 5304 7341 6
Size: 2110 3188 2037 455
Fragments arranged by size:
3188 2110 2037 455
BsaHI Gr'CG_yC
Cuts at: 1983 2139 3858 5221 5469 6171 7667 1983
Size: 156 1719 1363 248 702 1496 2106
Fragments arranged by size:
2106 1719 1496 1363 702 248 156
BsaWI w'CCGG_w
Cuts at: 141 762 2012 4979 5500 6178 6452 6599 7010
Size: 621 1250 2967 521 678 274 147 411
Cuts at: 7010 141
Size: 921
Fragments arranged by size:
2967 1250 921 678 621 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 1360 1390 1868 1898 3460 3490 4157
Size: 30 1227 30 478 30 1562 30 667
Cuts at: 4157 4187 4219 4249 4719 4749 103
Size: 30 32 30 470 30 3144
Fragments arranged by size:
3144 1562 1227 667 478 470 32 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 390 670 943 1006 1163 1516 1519 2053 3070
Size: 280 273 63 157 353 3 534 1017
Cuts at: 3070 3316 3639 3751 4084 4216 4318 4543 4588
Size: 246 323 112 333 132 102 225 45
Cuts at: 4588 4600 4615 4618 390
Size: 12 15 3 3562
Fragments arranged by size:
3562 1017 534 353 333 323 280 273
246 225 157 132 112 102 63 45
15 12 3 3
BseYI C'CCAG_C
Cuts at: 167 521 1978 2835 2858 3050 3471 3501 3688
Size: 354 1457 857 23 192 421 30 187
Cuts at: 3688 4518 4654 5215 5973 6550 7046 167
Size: 830 136 561 758 577 496 911
Fragments arranged by size:
1457 911 857 830 758 577 561 496
421 354 192 187 136 30 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1002 1780 1892 3440 4613 727
Size: 275 778 112 1548 1173 3904
Fragments arranged by size:
3904 1548 1173 778 275 112
BsiEI CG_ry'CG
Cuts at: 104 5332 5378 6586 104
Size: 5228 46 1208 1308
Fragments arranged by size:
5228 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2000 2326 2592 2663 3643 3702 4037 4310 4558
Size: 326 266 71 980 59 335 273 248
Cuts at: 4558 4804 5582 5772 6564 2000
Size: 246 778 190 792 3226
Fragments arranged by size:
3226 980 792 778 335 326 273 266
248 246 190 71 59
Bsp1286I G_dGCh'C
Cuts at: 151 400 513 1210 1355 1604 1718 2000 2326
Size: 249 113 697 145 249 114 282 326
Cuts at: 2326 2493 2592 2663 2941 3643 3702 4037 4210
Size: 167 99 71 278 702 59 335 173
Cuts at: 4210 4296 4310 4455 4467 4558 4624 4804 5415
Size: 86 14 145 12 91 66 180 611
Cuts at: 5415 5508 5582 5772 5834 6564 151
Size: 93 74 190 62 730 1377
Fragments arranged by size:
1377 730 702 697 611 335 326 282
278 249 249 190 180 173 167 145
145 114 113 99 93 91 86 74
71 66 62 59 14 12
BspEI T'CCGG_A
Cuts at: 762 2012 7010 762
Size: 1250 4998 1542
Fragments arranged by size:
4998 1542 1250
BspHI T'CATG_A
Cuts at: 1814 4310 6966 1814
Size: 2496 2656 2638
Fragments arranged by size:
2656 2638 2496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2351 2699 2865 3158 3669 4334 4840 5054 5356
Size: 348 166 293 511 665 506 214 302
Cuts at: 5356 5737 2351
Size: 381 4404
Fragments arranged by size:
4404 665 511 506 381 348 302 293
214 166
BsrBI CCG'CTC
Cuts at: 50 2136 2514 6082 6136 7620 7785 50
Size: 2086 378 3568 54 1484 165 55
Fragments arranged by size:
3568 2086 1484 378 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1062 3038 5702 1062
Size: 1976 2664 3150
Fragments arranged by size:
3150 2664 1976
BsrFI r'CCGG_y
Cuts at: 734 1542 2006 2333 3427 3452 4686 5788 5969
Size: 808 464 327 1094 25 1234 1102 181
Cuts at: 5969 6178 734
Size: 209 2346
Fragments arranged by size:
2346 1234 1102 1094 808 464 327 209
181 25
BsrGI T'GTAC_A
Cuts at: 3479 3479
Size: 7790
BssSI C'ACGA_G
Cuts at: 2765 6061 6419 2765
Size: 3296 358 4136
Fragments arranged by size:
4136 3296 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1350 2343 3030 3906 4096 4371 5167 1350
Size: 993 687 876 190 275 796 3973
Fragments arranged by size:
3973 993 876 796 687 275 190
BstBI TT'CG_AA
Cuts at: 6151 6151
Size: 7790
BstEII G'GTnAC_C
Cuts at: 3229 4335 3229
Size: 1106 6684
BstXI CCAn_nnnn'nTGG
Cuts at: 840 918 2617 3627 840
Size: 78 1699 1010 5003
Fragments arranged by size:
5003 1699 1010 78
BstYI r'GATC_y
Cuts at: 1 242 431 815 1184 3218 4221 4403 4815
Size: 241 189 384 369 2034 1003 182 412
Cuts at: 4815 5282 5305 5640 5886 6898 6984 6996 7342
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7342 1
Size: 449
Fragments arranged by size:
2034 1012 1003 467 449 412 384 369
346 335 246 241 189 182 86 23
12
BstZ17I GTA'TAC
Cuts at: 2357 2357
Size: 7790
Bsu36I CC'TnA_GG
Cuts at: 2852 3523 4067 4139 4280 2852
Size: 671 544 72 141 6362
Fragments arranged by size:
6362 671 544 141 72
BtgI C'CryG_G
Cuts at: 107 254 1839 3732 107
Size: 147 1585 1893 4165
Fragments arranged by size:
4165 1893 1585 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 183 201 947 2904 4526 5740 5921 6084 183
Size: 18 746 1957 1622 1214 181 163 1889
Fragments arranged by size:
1957 1889 1622 1214 746 181 163 18
BtsI GCAGTG_nn'
Cuts at: 1958 2782 3712 3796 3883 3946 4128 5033 1958
Size: 824 930 84 87 63 182 905 4715
Fragments arranged by size:
4715 930 905 824 182 87 84 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1344 1379 1344
Size: 35 7755
DraI TTT'AAA
Cuts at: 1317 4789 1317
Size: 3472 4318
DraIII CAC_nnn'GTG
Cuts at: 2631 2631
Size: 7790
DrdI GACnn_nn'nnGTC
Cuts at: 2287 5496 6354 2287
Size: 3209 858 3723
Fragments arranged by size:
3723 3209 858
EaeI y'GGCC_r
Cuts at: 732 857 1304 2607 3611 5375 5549 5940 5967
Size: 125 447 1303 1004 1764 174 391 27
Cuts at: 5967 7312 732
Size: 1345 1210
Fragments arranged by size:
1764 1345 1303 1210 1004 447 391 174
125 27
EagI C'GGCC_G
Cuts at: 5375 5375
Size: 7790
EarI CTCTTCn'nnn_
Cuts at: 1493 2126 4586 5813 6023 1493
Size: 633 2460 1227 210 3260
Fragments arranged by size:
3260 2460 1227 633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 235 444 1240 2478 4027 4500 4705 5998 6308
Size: 209 796 1238 1549 473 205 1293 310
Cuts at: 6308 6454 7600 235
Size: 146 1146 425
Fragments arranged by size:
1549 1293 1238 1146 796 473 425 310
209 205 146
EcoICRI GAG'CTC
Cuts at: 2590 2661 3700 4035 4308 4556 4802 2590
Size: 71 1039 335 273 248 246 5578
Fragments arranged by size:
5578 1039 335 273 248 246 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 635 1000 1401 1680 2179 2310 2413 2467
Size: 356 365 401 279 499 131 103 54
Cuts at: 2467 2522 2775 2851 3097 3319 3366 3421 3889
Size: 55 253 76 246 222 47 55 468
Cuts at: 3889 4019 4177 4246 4417 4438 4444 4489 4618
Size: 130 158 69 171 21 6 45 129
Cuts at: 4618 4688 5615 6047 6794 7062 279
Size: 70 927 432 747 268 1007
Fragments arranged by size:
1007 927 747 499 468 432 401 365
356 279 268 253 246 222 171 158
131 130 129 103 76 70 69 55
55 54 47 45 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2563 3718 4114 4330 2563
Size: 1155 396 216 6023
Fragments arranged by size:
6023 1155 396 216
EcoO109I rG'GnC_Cy
Cuts at: 829 895 2151 2772 3261 3282 3519 3528 3933
Size: 66 1256 621 489 21 237 9 405
Cuts at: 3933 4293 4323 4464 4620 4621 4708 4929 829
Size: 360 30 141 156 1 87 221 3690
Fragments arranged by size:
3690 1256 621 489 405 360 237 221
156 141 87 66 30 21 9 1
EcoRI G'AATT_C
Cuts at: 4794 4794
Size: 7790
EcoRV GAT'ATC
Cuts at: 1584 1584
Size: 7790
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5809 5809
Size: 7790
FspI TGC'GCA
Cuts at: 3515 5571 3515
Size: 2056 5734
HaeII r_GCGC'y
Cuts at: 528 1986 2220 3861 5224 5472 6494 7473 528
Size: 1458 234 1641 1363 248 1022 979 845
Fragments arranged by size:
1641 1458 1363 1022 979 845 248 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 946 978 1080 1112 1418 1450 1868
Size: 32 811 32 102 32 306 32 418
Cuts at: 1868 1900 2009 2041 2951 2983 3415 3447 3520
Size: 32 109 32 910 32 432 32 73
Cuts at: 3520 3552 3765 3797 4219 4251 5632 5662 5664
Size: 32 213 32 422 32 1381 30 2
Cuts at: 5664 5694 103
Size: 30 2199
Fragments arranged by size:
2199 1381 910 811 432 422 418 306
213 109 102 73 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 959 1950 4256 4829 959
Size: 991 2306 573 3920
Fragments arranged by size:
3920 2306 991 573
KasI G'GCGC_C
Cuts at: 1982 3857 5220 5468 1982
Size: 1875 1363 248 4304
Fragments arranged by size:
4304 1875 1363 248
KpnI G_GTAC'C
Cuts at: 4810 4810
Size: 7790
MluI A'CGCG_T
Cuts at: 7223 7223
Size: 7790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 146 1504 1529 2798 4250 6461 6645 146
Size: 1358 25 1269 1452 2211 184 1291
Fragments arranged by size:
2211 1452 1358 1291 1269 184 25
MscI TGG'CCA
Cuts at: 859 5551 859
Size: 4692 3098
MslI CAynn'nnrTG
Cuts at: 144 916 1024 1055 1339 1574 1597 1688 1813
Size: 772 108 31 284 235 23 91 125
Cuts at: 1813 1831 2122 2306 5779 5906 144
Size: 18 291 184 3473 127 2028
Fragments arranged by size:
3473 2028 772 291 284 235 184 127
125 108 91 31 23 18
MspA1I CmG'CkG
Cuts at: 256 1457 1913 2098 2606 2835 2871 3594 3965
Size: 1201 456 185 508 229 36 723 371
Cuts at: 3965 4368 4901 5215 5575 6588 6833 256
Size: 403 533 314 360 1013 245 1213
Fragments arranged by size:
1213 1201 1013 723 533 508 456 403
371 360 314 245 229 185 36
NaeI GCC'GGC
Cuts at: 736 2008 2335 3429 5971 736
Size: 1272 327 1094 2542 2555
Fragments arranged by size:
2555 2542 1272 1094 327
NarI GG'CG_CC
Cuts at: 1983 3858 5221 5469 1983
Size: 1875 1363 248 4304
Fragments arranged by size:
4304 1875 1363 248
NcoI C'CATG_G
Cuts at: 107 1839 3732 107
Size: 1732 1893 4165
Fragments arranged by size:
4165 1893 1732
NdeI CA'TA_TG
Cuts at: 1077 1346 4360 1077
Size: 269 3014 4507
Fragments arranged by size:
4507 3014 269
NgoMIV G'CCGG_C
Cuts at: 734 2006 2333 3427 5969 734
Size: 1272 327 1094 2542 2555
Fragments arranged by size:
2555 2542 1272 1094 327
NruI TCG'CGA
Cuts at: 202 202
Size: 7790
NspI r_CATG'y
Cuts at: 1338 4843 5343 6250 1338
Size: 3505 500 907 2878
Fragments arranged by size:
3505 2878 907 500
PciI A'CATG_T
Cuts at: 6246 6246
Size: 7790
PflMI CCAn_nnn'nTGG
Cuts at: 383 1523 2631 3965 383
Size: 1140 1108 1334 4208
Fragments arranged by size:
4208 1334 1140 1108
PfoI T'CCnGG_A
Cuts at: 977 3343 4580 977
Size: 2366 1237 4187
Fragments arranged by size:
4187 2366 1237
PmeI GTTT'AAAC
Cuts at: 4789 4789
Size: 7790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1360 1392 6929 6961 1360
Size: 32 5537 32 2189
Fragments arranged by size:
5537 2189 32 32
PpuMI rG'GwC_Cy
Cuts at: 3282 3519 3528 4323 3282
Size: 237 9 795 6749
Fragments arranged by size:
6749 795 237 9
PshAI GACnn'nnGTC
Cuts at: 3716 3716
Size: 7790
PspOMI G'GGCC_C
Cuts at: 4292 4463 4620 4292
Size: 171 157 7462
Fragments arranged by size:
7462 171 157
PspXI vC'TCGA_Gb
Cuts at: 4330 4330
Size: 7790
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1544 1576 1544
Size: 32 7758
PstI C_TGCA'G
Cuts at: 755 1289 2543 2810 3026 3593 3707 3902 3941
Size: 534 1254 267 216 567 114 195 39
Cuts at: 3941 4092 4376 4837 5522 755
Size: 151 284 461 685 3023
Fragments arranged by size:
3023 1254 685 567 534 461 284 267
216 195 151 114 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7790
PvuII CAG'CTG
Cuts at: 2098 2835 3594 3965 4368 5215 5575 2098
Size: 737 759 371 403 847 360 4313
Fragments arranged by size:
4313 847 759 737 403 371 360
RsrII CG'GwC_CG
Cuts at: 5985 5985
Size: 7790
SacI G_AGCT'C
Cuts at: 2592 2663 3702 4037 4310 4558 4804 2592
Size: 71 1039 335 273 248 246 5578
Fragments arranged by size:
5578 1039 335 273 248 246 71
SacII CC_GC'GG
Cuts at: 257 257
Size: 7790
SalI G'TCGA_C
Cuts at: 4827 4827
Size: 7790
SanDI GG'GwC_CC
Cuts at: 3528 3528
Size: 7790
SapI GCTCTTCn'nnn_
Cuts at: 2126 4586 5813 6023 2126
Size: 2460 1227 210 3893
Fragments arranged by size:
3893 2460 1227 210
SbfI CC_TGCA'GG
Cuts at: 4837 4837
Size: 7790
ScaI AGT'ACT
Cuts at: 7307 7307
Size: 7790
SexAI A'CCwGG_T
Cuts at: 1702 1702
Size: 7790
SfcI C'TryA_G
Cuts at: 33 444 751 811 1285 2539 2641 2806 3022
Size: 411 307 60 474 1254 102 165 216
Cuts at: 3022 3322 3589 3703 3898 3937 4048 4088 4372
Size: 300 267 114 195 39 111 40 284
Cuts at: 4372 4833 5518 6511 6702 33
Size: 461 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 474 461 411 307
300 284 267 216 195 191 165 114
111 102 60 40 39
SfoI GGC'GCC
Cuts at: 1984 3859 5222 5470 1984
Size: 1875 1363 248 4304
Fragments arranged by size:
4304 1875 1363 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7790
SmaI CCC'GGG
Cuts at: 1273 3193 4291 4812 1273
Size: 1920 1098 521 4251
Fragments arranged by size:
4251 1920 1098 521
SmlI C'TyrA_G
Cuts at: 1097 3109 3169 3373 3852 4330 4945 5860 6352
Size: 2012 60 204 479 478 615 915 492
Cuts at: 6352 6614 1097
Size: 262 2273
Fragments arranged by size:
2273 2012 915 615 492 479 478 262
204 60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7790
SphI G_CATG'C
Cuts at: 4843 5343 4843
Size: 500 7290
SrfI GCCC'GGGC
Cuts at: 3193 3193
Size: 7790
SspI AAT'ATT
Cuts at: 552 552
Size: 7790
StuI AGG'CCT
Cuts at: 2749 3358 2749
Size: 609 7181
StyI C'CwwG_G
Cuts at: 107 1190 1839 1923 2360 2942 3732 4298 4924
Size: 1083 649 84 437 582 790 566 626
Cuts at: 4924 5278 107
Size: 354 2619
Fragments arranged by size:
2619 1083 790 649 626 582 566 437
354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4647 4675 5373 5506 5965 6174 4647
Size: 28 698 133 459 209 6263
Fragments arranged by size:
6263 698 459 209 133 28
TatI w'GTAC_w
Cuts at: 1560 3479 7305 1560
Size: 1919 3826 2045
Fragments arranged by size:
3826 2045 1919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 4157 4189 4719 4751 7307 7339 490
Size: 32 3635 32 530 32 2556 32 941
Fragments arranged by size:
3635 2556 941 530 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5587 5587
Size: 7790
XbaI T'CTAG_A
Cuts at: 4821 4821
Size: 7790
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 257 1362 2632 3803 3987 5285 257
Size: 1105 1270 1171 184 1298 2762
Fragments arranged by size:
2762 1298 1270 1171 1105 184
XhoI C'TCGA_G
Cuts at: 1097 4330 1097
Size: 3233 4557
XmaI C'CCGG_G
Cuts at: 1271 3191 4289 4810 1271
Size: 1920 1098 521 4251
Fragments arranged by size:
4251 1920 1098 521
XmnI GAAnn'nnTTC
Cuts at: 1375 1375
Size: 7790
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsmBI BssHII ClaI FseI FspAI HindIII
HpaI MfeI NheI NotI NsiI PacI PasI PmlI
PsiI SfiI SgrAI SnaBI SwaI ZraI