Restriction map for
pF1KE9652
(Circular) (Six-base) MAPSORT of: pF1KE9652.seq Check: 4714 from: 1 to: 10856
pF1KE9652 10856 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1125 3488 1125
Size: 2363 8493
AccI GT'mk_AC
Cuts at: 1035 2039 5539 6073 7565 7894 1035
Size: 1004 3500 534 1492 329 3997
Fragments arranged by size:
3997 3500 1492 1004 534 329
Acc65I G'GTAC_C
Cuts at: 7872 7872
Size: 10856
AclI AA'CG_TT
Cuts at: 10696 10696
Size: 10856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 2117 3440 3675 4163 8681 9113 9860 10128
Size: 1183 1323 235 488 4518 432 747 268
Cuts at: 10128 934
Size: 1662
Fragments arranged by size:
4518 1662 1323 1183 747 488 432 268
235
AfeI AGC'GCT
Cuts at: 2206 2206
Size: 10856
AflII C'TTAA_G
Cuts at: 592 3181 592
Size: 2589 8267
AflIII A'CryG_T
Cuts at: 7442 8208 9312 10289 7442
Size: 766 1104 977 8009
Fragments arranged by size:
8009 1104 977 766
AgeI A'CCGG_T
Cuts at: 9244 9244
Size: 10856
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 10856
AleI CACnn'nnGTG
Cuts at: 2767 4589 7496 2767
Size: 1822 2907 6127
Fragments arranged by size:
6127 2907 1822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2504 5971 6712 2504
Size: 3467 741 6648
Fragments arranged by size:
6648 3467 741
AlwNI CAG_nnn'CTG
Cuts at: 619 675 880 3769 5698 8161 9728 619
Size: 56 205 2889 1929 2463 1567 1747
Fragments arranged by size:
2889 2463 1929 1747 1567 205 56
ApaI G_GGCC'C
Cuts at: 908 7365 908
Size: 6457 4399
ApaLI G'TGCA_C
Cuts at: 7544 9626 7544
Size: 2082 8774
ApoI r'AATT_y
Cuts at: 144 285 638 2107 2428 2737 3309 3439 4360
Size: 141 353 1469 321 309 572 130 921
Cuts at: 4360 6674 7860 10209 10465 144
Size: 2314 1186 2349 256 535
Fragments arranged by size:
2349 2314 1469 1186 921 572 535 353
321 309 256 141 130
AseI AT'TA_AT
Cuts at: 20 3755 20
Size: 3735 7121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10856
AvaI C'yCGr_G
Cuts at: 1550 1796 3734 6133 7876 1550
Size: 246 1938 2399 1743 4530
Fragments arranged by size:
4530 2399 1938 1743 246
BamHI G'GATC_C
Cuts at: 4760 7049 7881 4760
Size: 2289 832 7735
Fragments arranged by size:
7735 2289 832
BanI G'GyrC_C
Cuts at: 622 1675 2450 3231 7872 8286 8534 8569 622
Size: 1053 775 781 4641 414 248 35 2909
Fragments arranged by size:
4641 2909 1053 781 775 414 248 35
BanII G_rGCy'C
Cuts at: 908 1355 1524 2078 2261 2336 5335 5738 7365
Size: 447 169 554 183 75 2999 403 1627
Cuts at: 7365 7647 7870 8900 908
Size: 282 223 1030 2864
Fragments arranged by size:
2999 2864 1627 1030 554 447 403 282
223 183 169 75
BbeI G_GCGC'C
Cuts at: 8290 8538 8290
Size: 248 10608
BbsI GAAGACnn'nnnn_
Cuts at: 3207 3711 4570 3207
Size: 504 859 9493
Fragments arranged by size:
9493 859 504
BciVI GTATCCnnnnn_n'
Cuts at: 2180 6252 8754 9515 10856 2180
Size: 4072 2502 761 1341 2180
Fragments arranged by size:
4072 2502 2180 1341 761
BclI T'GATC_A
Cuts at: 2876 8376 2876
Size: 5500 5356
BglI GCCn_nnn'nGGC
Cuts at: 631 4246 5889 631
Size: 3615 1643 5598
Fragments arranged by size:
5598 3615 1643
BglII A'GATC_T
Cuts at: 4020 4103 5264 8371 10408 4020
Size: 83 1161 3107 2037 4468
Fragments arranged by size:
4468 3107 2037 1161 83
BlpI GC'TnA_GC
Cuts at: 668 7968 668
Size: 7300 3556
Bme1580I G_kGCm'C
Cuts at: 627 908 1530 7365 7548 8481 8574 9630 627
Size: 281 622 5835 183 933 93 1056 1853
Fragments arranged by size:
5835 1853 1056 933 622 281 183 93
BmrI ACTGGGnnnn_n'
Cuts at: 2009 2736 4939 6152 6813 7430 8068 8149 8484
Size: 727 2203 1213 661 617 638 81 335
Cuts at: 8484 10628 2009
Size: 2144 2237
Fragments arranged by size:
2237 2203 2144 1213 727 661 638 617
335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5283 5772 9223 5283
Size: 489 3451 6916
Fragments arranged by size:
6916 3451 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 350 525 572 841 964 1016 4981 5720
Size: 6 175 47 269 123 52 3965 739
Cuts at: 5720 6184 6316 6363 6369 6725 6743 7015 7060
Size: 464 132 47 6 356 18 272 45
Cuts at: 7060 7138 7554 7815 344
Size: 78 416 261 3385
Fragments arranged by size:
3965 3385 739 464 416 356 272 269
261 175 132 123 78 52 47 47
45 18 6 6
Bpu10I CC'TnA_GC
Cuts at: 493 1937 2912 4166 6527 493
Size: 1444 975 1254 2361 4822
Fragments arranged by size:
4822 2361 1444 1254 975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 2983 3109 3127 3910 6351 7659 8032 8911
Size: 1916 126 18 783 2441 1308 373 879
Cuts at: 8911 9403 9701 1067
Size: 492 298 2222
Fragments arranged by size:
2441 2222 1916 1308 879 783 492 373
298 126 18
BsaI GGTCTCn'nnnn_
Cuts at: 4540 4880 7036 7430 10560 4540
Size: 340 2156 394 3130 4836
Fragments arranged by size:
4836 3130 2156 394 340
BsaAI yAC'GTr
Cuts at: 2277 8209 2277
Size: 5932 4924
BsaBI GATnn'nnATC
Cuts at: 6 586 5525 6944 8370 10407 6
Size: 580 4939 1419 1426 2037 455
Fragments arranged by size:
4939 2037 1426 1419 580 455
BsaHI Gr'CG_yC
Cuts at: 448 2841 5358 8287 8535 9237 10733 448
Size: 2393 2517 2929 248 702 1496 571
Fragments arranged by size:
2929 2517 2393 1496 702 571 248
BsaWI w'CCGG_w
Cuts at: 4763 8045 8566 9244 9518 9665 10076 4763
Size: 3282 521 678 274 147 411 5543
Fragments arranged by size:
5543 3282 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1701 1731 6988 7018 7111 7141 7813 7843 1701
Size: 30 5257 30 93 30 672 30 4714
Fragments arranged by size:
5257 4714 672 93 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 528 600 1409 1606 1660 3636 5275 5761 6151
Size: 72 809 197 54 1976 1639 486 390
Cuts at: 6151 6799 7732 528
Size: 648 933 3652
Fragments arranged by size:
3652 1976 1639 933 809 648 486 390
197 72 54
BseYI C'CCAG_C
Cuts at: 829 1199 1217 1576 1627 4635 5061 5691 8281
Size: 370 18 359 51 3008 426 630 2590
Cuts at: 8281 9039 9616 10112 829
Size: 758 577 496 1573
Fragments arranged by size:
3008 2590 1573 758 630 577 496 426
370 359 51 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 1769 6046 6788 1159
Size: 610 4277 742 5227
Fragments arranged by size:
5227 4277 742 610
BsiEI CG_ry'CG
Cuts at: 104 8398 8444 9652 104
Size: 8294 46 1208 1308
Fragments arranged by size:
8294 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2078 2365 6024 6191 7548 7870 8648 8838 9630
Size: 287 3659 167 1357 322 778 190 792
Cuts at: 9630 2078
Size: 3304
Fragments arranged by size:
3659 3304 1357 792 778 322 287 190
167
BsmI GAATG_Cn'
Cuts at: 1098 1817 4859 1098
Size: 719 3042 7095
Fragments arranged by size:
7095 3042 719
BsmBI CGTCTCn'nnnn_
Cuts at: 1821 1821
Size: 10856
Bsp1286I G_dGCh'C
Cuts at: 627 908 1355 1524 1530 2078 2261 2336 2365
Size: 281 447 169 6 548 183 75 29
Cuts at: 2365 5335 5738 6024 6191 7365 7548 7647 7870
Size: 2970 403 286 167 1174 183 99 223
Cuts at: 7870 8481 8574 8648 8838 8900 9630 627
Size: 611 93 74 190 62 730 1853
Fragments arranged by size:
2970 1853 1174 730 611 548 447 403
286 281 223 190 183 183 169 167
99 93 75 74 62 29 6
BspEI T'CCGG_A
Cuts at: 4763 10076 4763
Size: 5313 5543
BspHI T'CATG_A
Cuts at: 3468 10032 3468
Size: 6564 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1125 3325 3488 3784 3824 7906 8120 8422 8803
Size: 2200 163 296 40 4082 214 302 381
Cuts at: 8803 1125
Size: 3178
Fragments arranged by size:
4082 3178 2200 381 302 296 214 163
40
BsrBI CCG'CTC
Cuts at: 50 4392 6440 9148 9202 10686 10851 50
Size: 4342 2048 2708 54 1484 165 55
Fragments arranged by size:
4342 2708 2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1511 2645 2699 3043 8768 1511
Size: 1134 54 344 5725 3599
Fragments arranged by size:
5725 3599 1134 344 54
BsrFI r'CCGG_y
Cuts at: 727 3502 5429 8854 9035 9244 727
Size: 2775 1927 3425 181 209 2339
Fragments arranged by size:
3425 2775 2339 1927 209 181
BsrGI T'GTAC_A
Cuts at: 1239 1239
Size: 10856
BssSI C'ACGA_G
Cuts at: 4567 4697 6190 9127 9485 4567
Size: 130 1493 2937 358 5938
Fragments arranged by size:
5938 2937 1493 358 130
BstAPI GCAn_nnn'nTGC
Cuts at: 1023 1414 3389 4442 8233 1023
Size: 391 1975 1053 3791 3646
Fragments arranged by size:
3791 3646 1975 1053 391
BstBI TT'CG_AA
Cuts at: 9217 9217
Size: 10856
BstEII G'GTnAC_C
Cuts at: 5295 5295
Size: 10856
BstXI CCAn_nnnn'nTGG
Cuts at: 265 1386 4976 265
Size: 1121 3590 6145
Fragments arranged by size:
6145 3590 1121
BstYI r'GATC_y
Cuts at: 1 126 488 3138 4020 4103 4263 4760 5184
Size: 125 362 2650 882 83 160 497 424
Cuts at: 5184 5264 7049 7881 8348 8371 8706 8952 9964
Size: 80 1785 832 467 23 335 246 1012
Cuts at: 9964 10050 10062 10408 1
Size: 86 12 346 449
Fragments arranged by size:
2650 1785 1012 882 832 497 467 449
424 362 346 335 246 160 125 86
83 80 23 12
BstZ17I GTA'TAC
Cuts at: 6074 6074
Size: 10856
Bsu36I CC'TnA_GG
Cuts at: 4867 7715 4867
Size: 2848 8008
BtgI C'CryG_G
Cuts at: 107 1530 2682 2775 3488 4590 5649 5925 6776
Size: 1423 1152 93 713 1102 1059 276 851
Cuts at: 6776 107
Size: 4187
Fragments arranged by size:
4187 1423 1152 1102 1059 851 713 276
93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1169 8806 8987 9150 1169
Size: 7637 181 163 2875
Fragments arranged by size:
7637 2875 181 163
BtsI GCAGTG_nn'
Cuts at: 6055 6083 6094 7503 7705 8099 6055
Size: 28 11 1409 202 394 8812
Fragments arranged by size:
8812 1409 394 202 28 11
DraI TTT'AAA
Cuts at: 2433 5022 6179 7855 2433
Size: 2589 1157 1676 5434
Fragments arranged by size:
5434 2589 1676 1157
DraIII CAC_nnn'GTG
Cuts at: 3024 6195 3024
Size: 3171 7685
DrdI GACnn_nn'nnGTC
Cuts at: 3715 4415 6742 8562 9420 3715
Size: 700 2327 1820 858 5151
Fragments arranged by size:
5151 2327 1820 858 700
EaeI y'GGCC_r
Cuts at: 779 1585 2786 3552 4393 5088 5660 6200 8441
Size: 806 1201 766 841 695 572 540 2241
Cuts at: 8441 8615 9006 9033 10378 779
Size: 174 391 27 1345 1257
Fragments arranged by size:
2241 1345 1257 1201 841 806 766 695
572 540 391 174 27
EagI C'GGCC_G
Cuts at: 8441 8441
Size: 10856
EarI CTCTTCn'nnn_
Cuts at: 1335 1350 1615 1654 1729 1819 1901 2249 4082
Size: 15 265 39 75 90 82 348 1833
Cuts at: 4082 6410 6452 7007 7665 8879 9089 1335
Size: 2328 42 555 658 1214 210 3102
Fragments arranged by size:
3102 2328 1833 1214 658 555 348 265
210 90 82 75 42 39 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9064 9374 9520 10666 9064
Size: 310 146 1146 9254
Fragments arranged by size:
9254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2076 7868 2076
Size: 5792 5064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 350 525 572 841 934 964 1016 2117
Size: 6 175 47 269 93 30 52 1101
Cuts at: 2117 3440 3675 4163 4981 5720 6184 6316 6363
Size: 1323 235 488 818 739 464 132 47
Cuts at: 6363 6369 6725 6743 7015 7060 7138 7554 7815
Size: 6 356 18 272 45 78 416 261
Cuts at: 7815 8681 9113 9860 10128 344
Size: 866 432 747 268 1072
Fragments arranged by size:
1323 1101 1072 866 818 747 739 488
464 432 416 356 272 269 268 261
235 175 132 93 78 52 47 47
45 30 18 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3670 4870 5080 7405 3670
Size: 1200 210 2325 7121
Fragments arranged by size:
7121 2325 1200 210
EcoO109I rG'GnC_Cy
Cuts at: 597 789 904 905 1000 1340 1869 5209 5961
Size: 192 115 1 95 340 529 3340 752
Cuts at: 5961 6153 7330 7995 597
Size: 192 1177 665 3458
Fragments arranged by size:
3458 3340 1177 752 665 529 340 192
192 115 95 1
EcoRI G'AATT_C
Cuts at: 285 2107 3439 4360 7860 285
Size: 1822 1332 921 3500 3281
Fragments arranged by size:
3500 3281 1822 1332 921
EcoRV GAT'ATC
Cuts at: 4743 4743
Size: 10856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4191 4830 8875 4191
Size: 639 4045 6172
Fragments arranged by size:
6172 4045 639
FspI TGC'GCA
Cuts at: 3382 8637 3382
Size: 5255 5601
HaeII r_GCGC'y
Cuts at: 2208 5793 8290 8538 9560 10539 2208
Size: 3585 2497 248 1022 979 2525
Fragments arranged by size:
3585 2525 2497 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2175 2207 2831 2863 3558 3590 4005 4037 4057
Size: 32 624 32 695 32 415 32 20
Cuts at: 4057 4089 4486 4518 4539 4571 5125 5157 6301
Size: 32 397 32 21 32 554 32 1144
Cuts at: 6301 6333 6694 6726 6778 6810 7741 7773 8698
Size: 32 361 32 52 32 931 32 925
Cuts at: 8698 8728 8730 8760 2175
Size: 30 2 30 4271
Fragments arranged by size:
4271 1144 931 925 695 624 554 415
397 361 52 32 32 32 32 32
32 32 32 32 32 32 32 30
30 21 20 2
HincII GTy'rAC
Cuts at: 5163 6896 7895 5163
Size: 1733 999 8124
Fragments arranged by size:
8124 1733 999
HindIII A'AGCT_T
Cuts at: 3449 3538 4173 3449
Size: 89 635 10132
Fragments arranged by size:
10132 635 89
HpaI GTT'AAC
Cuts at: 6896 6896
Size: 10856
KasI G'GCGC_C
Cuts at: 8286 8534 8286
Size: 248 10608
KpnI G_GTAC'C
Cuts at: 7876 7876
Size: 10856
MfeI C'AATT_G
Cuts at: 5108 5108
Size: 10856
MluI A'CGCG_T
Cuts at: 10289 10289
Size: 10856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2534 3917 4017 4993 5111 7473 9527 9711 2534
Size: 1383 100 976 118 2362 2054 184 3679
Fragments arranged by size:
3679 2362 2054 1383 976 184 118 100
MscI TGG'CCA
Cuts at: 2788 3554 5662 6202 8617 2788
Size: 766 2108 540 2415 5027
Fragments arranged by size:
5027 2415 2108 766 540
MslI CAynn'nnrTG
Cuts at: 1016 2249 2767 3370 4427 4567 4589 5030 7028
Size: 1233 518 603 1057 140 22 441 1998
Cuts at: 7028 7496 8845 8972 1016
Size: 468 1349 127 2900
Fragments arranged by size:
2900 1998 1349 1233 1057 603 518 468
441 140 127 22
MspA1I CmG'CkG
Cuts at: 532 849 1068 1131 1532 3766 4639 5698 6811
Size: 317 219 63 401 2234 873 1059 1113
Cuts at: 6811 7967 8281 8641 9654 9899 532
Size: 1156 314 360 1013 245 1489
Fragments arranged by size:
2234 1489 1156 1113 1059 1013 873 401
360 317 314 245 219 63
NaeI GCC'GGC
Cuts at: 3504 9037 3504
Size: 5533 5323
NarI GG'CG_CC
Cuts at: 8287 8535 8287
Size: 248 10608
NcoI C'CATG_G
Cuts at: 107 2682 2775 3488 5649 5925 6776 107
Size: 2575 93 713 2161 276 851 4187
Fragments arranged by size:
4187 2575 2161 851 713 276 93
NdeI CA'TA_TG
Cuts at: 4041 4936 4041
Size: 895 9961
NgoMIV G'CCGG_C
Cuts at: 3502 9035 3502
Size: 5533 5323
NspI r_CATG'y
Cuts at: 1021 3351 7909 8409 9316 1021
Size: 2330 4558 500 907 2561
Fragments arranged by size:
4558 2561 2330 907 500
PasI CC'CwG_GG
Cuts at: 793 892 948 793
Size: 99 56 10701
Fragments arranged by size:
10701 99 56
PciI A'CATG_T
Cuts at: 9312 9312
Size: 10856
PflMI CCAn_nnn'nTGG
Cuts at: 994 1324 4226 5698 5876 6660 994
Size: 330 2902 1472 178 784 5190
Fragments arranged by size:
5190 2902 1472 784 330 178
PfoI T'CCnGG_A
Cuts at: 6271 6720 6271
Size: 449 10407
PmeI GTTT'AAAC
Cuts at: 7855 7855
Size: 10856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9995 10027 9995
Size: 32 10824
PpuMI rG'GwC_Cy
Cuts at: 789 1000 1869 5209 5961 6153 789
Size: 211 869 3340 752 192 5492
Fragments arranged by size:
5492 3340 869 752 211 192
PsiI TTA'TAA
Cuts at: 4805 4805
Size: 10856
PspOMI G'GGCC_C
Cuts at: 904 7361 904
Size: 6457 4399
PspXI vC'TCGA_Gb
Cuts at: 1550 1550
Size: 10856
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1352 1384 1352
Size: 32 10824
PstI C_TGCA'G
Cuts at: 924 1136 1316 3485 3821 5418 6545 6801 7903
Size: 212 180 2169 336 1597 1127 256 1102
Cuts at: 7903 8588 924
Size: 685 3192
Fragments arranged by size:
3192 2169 1597 1127 1102 685 336 256
212 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10856
PvuII CAG'CTG
Cuts at: 532 849 1068 1131 3766 4639 5698 8281 8641
Size: 317 219 63 2635 873 1059 2583 360
Cuts at: 8641 532
Size: 2747
Fragments arranged by size:
2747 2635 2583 1059 873 360 317 219
63
RsrII CG'GwC_CG
Cuts at: 9051 9051
Size: 10856
SacI G_AGCT'C
Cuts at: 2078 7870 2078
Size: 5792 5064
SacII CC_GC'GG
Cuts at: 1533 1533
Size: 10856
SalI G'TCGA_C
Cuts at: 7893 7893
Size: 10856
SapI GCTCTTCn'nnn_
Cuts at: 1335 2249 7007 8879 9089 1335
Size: 914 4758 1872 210 3102
Fragments arranged by size:
4758 3102 1872 914 210
SbfI CC_TGCA'GG
Cuts at: 3485 3821 7903 3485
Size: 336 4082 6438
Fragments arranged by size:
6438 4082 336
ScaI AGT'ACT
Cuts at: 5644 10373 5644
Size: 4729 6127
SexAI A'CCwGG_T
Cuts at: 185 744 2348 4352 185
Size: 559 1604 2004 6689
Fragments arranged by size:
6689 2004 1604 559
SfcI C'TryA_G
Cuts at: 33 920 1132 1312 1358 1721 3056 3169 3481
Size: 887 212 180 46 363 1335 113 312
Cuts at: 3481 3817 5414 6541 6797 7468 7899 8584 9577
Size: 336 1597 1127 256 671 431 685 993
Cuts at: 9577 9768 33
Size: 191 1121
Fragments arranged by size:
1597 1335 1127 1121 993 887 685 671
431 363 336 312 256 212 191 180
113 46
SfoI GGC'GCC
Cuts at: 8288 8536 8288
Size: 248 10608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10856
SmaI CCC'GGG
Cuts at: 3736 7878 3736
Size: 4142 6714
SmlI C'TyrA_G
Cuts at: 592 1082 1550 1796 2998 3124 3142 3181 3925
Size: 490 468 246 1202 126 18 39 744
Cuts at: 3925 6330 7638 8011 8926 9418 9680 592
Size: 2405 1308 373 915 492 262 1768
Fragments arranged by size:
2405 1768 1308 1202 915 744 492 490
468 373 262 246 126 39 18
SnaBI TAC'GTA
Cuts at: 2277 2277
Size: 10856
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10856
SphI G_CATG'C
Cuts at: 7909 8409 7909
Size: 500 10356
SspI AAT'ATT
Cuts at: 4605 4605
Size: 10856
StyI C'CwwG_G
Cuts at: 107 216 242 498 648 785 1005 1098 1112
Size: 109 26 256 150 137 220 93 14
Cuts at: 1112 1335 2682 2775 3488 5205 5649 5925 6572
Size: 223 1347 93 713 1717 444 276 647
Cuts at: 6572 6776 7990 8344 107
Size: 204 1214 354 2619
Fragments arranged by size:
2619 1717 1347 1214 713 647 444 354
276 256 223 220 204 150 137 109
93 93 26 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 505 1231 2468 3249 3413 7078 7528 8439 8572
Size: 726 1237 781 164 3665 450 911 133
Cuts at: 8572 9031 9240 505
Size: 459 209 2121
Fragments arranged by size:
3665 2121 1237 911 781 726 459 450
209 164 133
TatI w'GTAC_w
Cuts at: 1239 2120 2819 4571 5642 6618 7257 10371 1239
Size: 881 699 1752 1071 976 639 3114 1724
Fragments arranged by size:
3114 1752 1724 1071 976 881 699 639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1608 1640 1701 1733 5493 5525 6988 7020 10373
Size: 32 61 32 3760 32 1463 32 3353
Cuts at: 10373 10405 1608
Size: 32 2059
Fragments arranged by size:
3760 3353 2059 1463 61 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1230 6604 8653 1230
Size: 5374 2049 3433
Fragments arranged by size:
5374 3433 2049
XbaI T'CTAG_A
Cuts at: 7273 7887 7273
Size: 614 10242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3927 4976 8351 3927
Size: 1049 3375 6432
Fragments arranged by size:
6432 3375 1049
XhoI C'TCGA_G
Cuts at: 1550 1796 1550
Size: 246 10610
XmaI C'CCGG_G
Cuts at: 3734 7876 3734
Size: 4142 6714
XmnI GAAnn'nnTTC
Cuts at: 508 3158 3310 4152 5783 508
Size: 2650 152 842 1631 5581
Fragments arranged by size:
5581 2650 1631 842 152
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AloI AscI AvrII BaeI BbvCI BcgI BfrBI
BmgBI BmtI BsiWI BssHII ClaI CspCI FseI FspAI
NheI NotI NruI NsiI PacI PmlI PshAI SanDI
SfiI SgrAI SrfI StuI SwaI ZraI