Restriction map for
pF1KE9696
(Circular) (Six-base) MAPSORT of: pF1KE9696.seq Check: 493 from: 1 to: 9101
pF1KE9696 9101 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 765 1291 4545 765
Size: 526 3254 5321
Fragments arranged by size:
5321 3254 526
AccI GT'mk_AC
Cuts at: 4239 5029 6139 4239
Size: 790 1110 7201
Fragments arranged by size:
7201 1110 790
Acc65I G'GTAC_C
Cuts at: 4994 6117 4994
Size: 1123 7978
AclI AA'CG_TT
Cuts at: 8941 8941
Size: 9101
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 1630 1783 3331 3815 4250 5450 5767 5883
Size: 1045 153 1548 484 435 1200 317 116
Cuts at: 5883 6926 7358 8105 8373 585
Size: 1043 432 747 268 1313
Fragments arranged by size:
1548 1313 1200 1045 1043 747 484 435
432 317 268 153 116
AfeI AGC'GCT
Cuts at: 4675 4675
Size: 9101
AflII C'TTAA_G
Cuts at: 3169 3169
Size: 9101
AflIII A'CryG_T
Cuts at: 1511 2306 6453 7557 8534 1511
Size: 795 4147 1104 977 2078
Fragments arranged by size:
4147 2078 1104 977 795
AgeI A'CCGG_T
Cuts at: 3641 7489 3641
Size: 3848 5253
AhdI GACnn_n'nnGTC
Cuts at: 4173 4173
Size: 9101
AleI CACnn'nnGTG
Cuts at: 3682 3919 3682
Size: 237 8864
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 541 573 2488 2520 2755 2787 541
Size: 32 1915 32 235 32 6855
Fragments arranged by size:
6855 1915 235 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 124 727 730 763 787 1057 2290 4413 4745
Size: 603 3 33 24 270 1233 2123 332
Cuts at: 4745 4885 5505 6406 7973 124
Size: 140 620 901 1567 1252
Fragments arranged by size:
2123 1567 1252 1233 901 620 603 332
270 140 33 24 3
ApaI G_GGCC'C
Cuts at: 2352 3477 5922 2352
Size: 1125 2445 5531
Fragments arranged by size:
5531 2445 1125
ApaLI G'TGCA_C
Cuts at: 7871 7871
Size: 9101
ApoI r'AATT_y
Cuts at: 334 2524 2584 3102 3117 3784 6105 8454 8710
Size: 2190 60 518 15 667 2321 2349 256
Cuts at: 8710 334
Size: 725
Fragments arranged by size:
2349 2321 2190 725 667 518 256 60
15
AseI AT'TA_AT
Cuts at: 20 20
Size: 9101
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9101
AvaI C'yCGr_G
Cuts at: 932 1029 3503 4298 4532 5159 5292 6065 6121
Size: 97 2474 795 234 627 133 773 56
Cuts at: 6121 932
Size: 3912
Fragments arranged by size:
3912 2474 795 773 627 234 133 97
56
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4978 5011 4978
Size: 33 9068
BamHI G'GATC_C
Cuts at: 6126 6126
Size: 9101
BanI G'GyrC_C
Cuts at: 975 2296 2517 3430 4956 4965 4994 5935 6117
Size: 1321 221 913 1526 9 29 941 182
Cuts at: 6117 6531 6779 6814 975
Size: 414 248 35 3262
Fragments arranged by size:
3262 1526 1321 941 913 414 248 221
182 35 29 9
BanII G_rGCy'C
Cuts at: 931 1335 1499 1865 2352 2861 3041 3477 3606
Size: 404 164 366 487 509 180 436 129
Cuts at: 3606 3739 5922 6065 6115 7145 931
Size: 133 2183 143 50 1030 2887
Fragments arranged by size:
2887 2183 1030 509 487 436 404 366
180 164 143 133 129 50
BbeI G_GCGC'C
Cuts at: 5939 6535 6783 5939
Size: 596 248 8257
Fragments arranged by size:
8257 596 248
BbsI GAAGACnn'nnnn_
Cuts at: 454 2171 2406 3989 4094 4166 4217 5328 454
Size: 1717 235 1583 105 72 51 1111 4227
Fragments arranged by size:
4227 1717 1583 1111 235 105 72 51
BbvCI CC'TCA_GC
Cuts at: 160 1058 4060 5806 160
Size: 898 3002 1746 3455
Fragments arranged by size:
3455 3002 1746 898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1580 1614 1580
Size: 34 9067
BciVI GTATCCnnnnn_n'
Cuts at: 6999 7760 9101 6999
Size: 761 1341 6999
Fragments arranged by size:
6999 1341 761
BclI T'GATC_A
Cuts at: 1455 2687 2697 6621 1455
Size: 1232 10 3924 3935
Fragments arranged by size:
3935 3924 1232 10
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 9101
BglII A'GATC_T
Cuts at: 5309 6616 8653 5309
Size: 1307 2037 5757
Fragments arranged by size:
5757 2037 1307
BlpI GC'TnA_GC
Cuts at: 770 3058 5710 6213 770
Size: 2288 2652 503 3658
Fragments arranged by size:
3658 2652 2288 503
Bme1580I G_kGCm'C
Cuts at: 2352 2359 2472 2520 3258 3435 3477 5830 5922
Size: 7 113 48 738 177 42 2353 92
Cuts at: 5922 6726 6819 7875 2352
Size: 804 93 1056 3578
Fragments arranged by size:
3578 2353 1056 804 738 177 113 93
92 48 42 7
BmgBI CAC'GTC
Cuts at: 3921 5730 3921
Size: 1809 7292
BmrI ACTGGGnnnn_n'
Cuts at: 2222 2502 3227 4056 6313 6394 6729 8873 2222
Size: 280 725 829 2257 81 335 2144 2450
Fragments arranged by size:
2450 2257 2144 829 725 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1373 7468 1373
Size: 6095 3006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 559 1319 2157 2312 2872 2938 3274 4665
Size: 398 760 838 155 560 66 336 1391
Cuts at: 4665 4751 4945 4991 5473 5515 5719 5978 161
Size: 86 194 46 482 42 204 259 3284
Fragments arranged by size:
3284 1391 838 760 560 482 398 336
259 204 194 155 86 66 46 42
Bpu10I CC'TnA_GC
Cuts at: 160 1058 1319 4060 4154 5806 160
Size: 898 261 2741 94 1652 3455
Fragments arranged by size:
3455 2741 1652 898 261 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2743 2983 3391 4828 6277 7156 7648 7946 2743
Size: 240 408 1437 1449 879 492 298 3898
Fragments arranged by size:
3898 1449 1437 879 492 408 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 8805 8805
Size: 9101
BsaAI yAC'GTr
Cuts at: 2081 6454 2081
Size: 4373 4728
BsaBI GATnn'nnATC
Cuts at: 6 667 1691 6615 8652 6
Size: 661 1024 4924 2037 455
Fragments arranged by size:
4924 2037 1024 661 455
BsaHI Gr'CG_yC
Cuts at: 5032 5936 6532 6780 7482 8978 5032
Size: 904 596 248 702 1496 5155
Fragments arranged by size:
5155 1496 904 702 596 248
BsaWI w'CCGG_w
Cuts at: 397 817 3641 3890 4259 6290 6811 7489 7763
Size: 420 2824 249 369 2031 521 678 274
Cuts at: 7763 7910 8321 397
Size: 147 411 1177
Fragments arranged by size:
2824 2031 1177 678 521 420 411 369
274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 1486 1516 1867 1897 1939 1969 2488
Size: 30 915 30 351 30 42 30 519
Cuts at: 2488 2518 4755 4785 5044 5074 541
Size: 30 2237 30 259 30 4568
Fragments arranged by size:
4568 2237 915 519 351 259 42 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 580 1213 1216 1432 1459 1462 1512 1963
Size: 237 633 3 216 27 3 50 451
Cuts at: 1963 2869 2935 3850 4039 4213 4216 4668 4680
Size: 906 66 915 189 174 3 452 12
Cuts at: 4680 5065 5530 5716 343
Size: 385 465 186 3728
Fragments arranged by size:
3728 915 906 633 465 452 451 385
237 216 189 186 174 66 50 27
12 3 3 3
BseYI C'CCAG_C
Cuts at: 2174 2723 2973 3508 3732 4323 4373 4657 4894
Size: 549 250 535 224 591 50 284 237
Cuts at: 4894 4899 5344 6526 7284 7861 8357 2174
Size: 5 445 1182 758 577 496 2918
Fragments arranged by size:
2918 1182 758 591 577 549 535 496
445 284 250 237 224 50 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2287 3943 4621 4731 5662 1642
Size: 645 1656 678 110 931 5081
Fragments arranged by size:
5081 1656 931 678 645 110
BsiEI CG_ry'CG
Cuts at: 104 3456 6643 6689 7897 104
Size: 3352 3187 46 1208 1308
Fragments arranged by size:
3352 3187 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1499 2861 3041 3335 4616 6115 6893 7083 7875
Size: 1362 180 294 1281 1499 778 190 792
Cuts at: 7875 1499
Size: 2725
Fragments arranged by size:
2725 1499 1362 1281 792 778 294 190
180
BsmBI CGTCTCn'nnnn_
Cuts at: 1213 4712 1213
Size: 3499 5602
Bsp1286I G_dGCh'C
Cuts at: 931 1335 1499 1865 2352 2359 2472 2520 2861
Size: 404 164 366 487 7 113 48 341
Cuts at: 2861 3041 3258 3335 3435 3477 3606 3739 4616
Size: 180 217 77 100 42 129 133 877
Cuts at: 4616 5830 5922 6065 6115 6726 6819 6893 7083
Size: 1214 92 143 50 611 93 74 190
Cuts at: 7083 7145 7875 931
Size: 62 730 2157
Fragments arranged by size:
2157 1214 877 730 611 487 404 366
341 217 190 180 164 143 133 129
113 100 93 92 77 74 62 50
48 42 7
BspEI T'CCGG_A
Cuts at: 817 4259 8321 817
Size: 3442 4062 1597
Fragments arranged by size:
4062 3442 1597
BspHI T'CATG_A
Cuts at: 611 3158 8277 611
Size: 2547 5119 1435
Fragments arranged by size:
5119 2547 1435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 765 1291 4545 4767 6151 6365 6667 7048 765
Size: 526 3254 222 1384 214 302 381 2818
Fragments arranged by size:
3254 2818 1384 526 381 302 222 214
BsrBI CCG'CTC
Cuts at: 50 2499 4920 7393 7447 8931 9096 50
Size: 2449 2421 2473 54 1484 165 55
Fragments arranged by size:
2473 2449 2421 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 7013 7013
Size: 9101
BsrFI r'CCGG_y
Cuts at: 1103 1145 3641 5559 5661 5810 5847 7099 7280
Size: 42 2496 1918 102 149 37 1252 181
Cuts at: 7280 7489 1103
Size: 209 2715
Fragments arranged by size:
2715 2496 1918 1252 209 181 149 102
42 37
BssSI C'ACGA_G
Cuts at: 1289 3122 7372 7730 1289
Size: 1833 4250 358 2660
Fragments arranged by size:
4250 2660 1833 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2970 6478 2970
Size: 3508 5593
BstBI TT'CG_AA
Cuts at: 7462 7462
Size: 9101
BstEII G'GTnAC_C
Cuts at: 5134 5134
Size: 9101
BstXI CCAn_nnnn'nTGG
Cuts at: 1258 1258
Size: 9101
BstYI r'GATC_y
Cuts at: 1 644 1628 3165 5309 6126 6593 6616 6951
Size: 643 984 1537 2144 817 467 23 335
Cuts at: 6951 7197 8209 8295 8307 8653 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2144 1537 1012 984 817 643 467 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1098 3436 4426 4721 1098
Size: 2338 990 295 5478
Fragments arranged by size:
5478 2338 990 295
BtgI C'CryG_G
Cuts at: 2272 3203 4998 5170 5358 5573 2272
Size: 931 1795 172 188 215 5800
Fragments arranged by size:
5800 1795 931 215 188 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3858 5754 7051 7232 7395 3858
Size: 1896 1297 181 163 5564
Fragments arranged by size:
5564 1896 1297 181 163
BtsI GCAGTG_nn'
Cuts at: 390 1089 1193 1670 1776 3533 3852 4279 4288
Size: 699 104 477 106 1757 319 427 9
Cuts at: 4288 6344 390
Size: 2056 3147
Fragments arranged by size:
3147 2056 1757 699 477 427 319 106
104 9
ClaI AT'CG_AT
Cuts at: 3413 3452 3413
Size: 39 9062
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1519 1554 1519
Size: 35 9066
DraI TTT'AAA
Cuts at: 5424 6100 5424
Size: 676 8425
DraIII CAC_nnn'GTG
Cuts at: 2016 3127 3330 4409 5261 2016
Size: 1111 203 1079 852 5856
Fragments arranged by size:
5856 1111 1079 852 203
DrdI GACnn_nn'nnGTC
Cuts at: 3657 6807 7665 3657
Size: 3150 858 5093
Fragments arranged by size:
5093 3150 858
EaeI y'GGCC_r
Cuts at: 797 1147 1190 3530 3615 3686 5612 5663 5816
Size: 350 43 2340 85 71 1926 51 153
Cuts at: 5816 5924 6686 6860 7251 7278 8623 797
Size: 108 762 174 391 27 1345 1275
Fragments arranged by size:
2340 1926 1345 1275 762 391 350 174
153 108 85 71 51 43 27
EagI C'GGCC_G
Cuts at: 6686 6686
Size: 9101
EarI CTCTTCn'nnn_
Cuts at: 247 870 1409 1724 1897 2632 2866 3662 3791
Size: 623 539 315 173 735 234 796 129
Cuts at: 3791 7124 7334 247
Size: 3333 210 2014
Fragments arranged by size:
3333 2014 796 735 623 539 315 234
210 173 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 474 933 5740 7309 7619 7765 8911 474
Size: 459 4807 1569 310 146 1146 664
Fragments arranged by size:
4807 1569 1146 664 459 310 146
EcoICRI GAG'CTC
Cuts at: 1497 2859 3039 6113 1497
Size: 1362 180 3074 4485
Fragments arranged by size:
4485 3074 1362 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 559 585 1319 1630 1783 2157 2312 2872
Size: 398 26 734 311 153 374 155 560
Cuts at: 2872 2938 3274 3331 3815 4250 4665 4751 4945
Size: 66 336 57 484 435 415 86 194
Cuts at: 4945 4991 5450 5473 5515 5719 5767 5883 5978
Size: 46 459 23 42 204 48 116 95
Cuts at: 5978 6926 7358 8105 8373 161
Size: 948 432 747 268 889
Fragments arranged by size:
948 889 747 734 560 484 459 435
432 415 398 374 336 311 268 204
194 155 153 116 95 86 66 57
48 46 42 26 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1721 3439 5497 5551 5635 5680 1721
Size: 1718 2058 54 84 45 5142
Fragments arranged by size:
5142 2058 1718 84 54 45
EcoO109I rG'GnC_Cy
Cuts at: 265 534 617 1042 1170 2452 3473 3513 4422
Size: 269 83 425 128 1282 1021 40 909
Cuts at: 4422 4527 5155 5863 5918 5919 5963 6240 265
Size: 105 628 708 55 1 44 277 3126
Fragments arranged by size:
3126 1282 1021 909 708 628 425 277
269 128 105 83 55 44 40 1
EcoRI G'AATT_C
Cuts at: 2584 3117 6105 2584
Size: 533 2988 5580
Fragments arranged by size:
5580 2988 533
EcoRV GAT'ATC
Cuts at: 855 855
Size: 9101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5463 7120 5463
Size: 1657 7444
FseI GG_CCGG'CC
Cuts at: 1107 1149 1107
Size: 42 9059
FspI TGC'GCA
Cuts at: 6882 6882
Size: 9101
HaeII r_GCGC'y
Cuts at: 4664 4677 5506 5906 5939 6535 6783 7805 8784
Size: 13 829 400 33 596 248 1022 979
Cuts at: 8784 4664
Size: 4981
Fragments arranged by size:
4981 1022 979 829 596 400 248 33
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 763 795 1867 1899 3320 3352 3998 4030 4159
Size: 32 1072 32 1421 32 646 32 129
Cuts at: 4159 4191 5158 5190 6943 6973 6975 7005 763
Size: 32 967 32 1753 30 2 30 2859
Fragments arranged by size:
2859 1753 1421 1072 967 646 129 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4137 4562 5030 6140 4137
Size: 425 468 1110 7098
Fragments arranged by size:
7098 1110 468 425
HindIII A'AGCT_T
Cuts at: 955 2624 955
Size: 1669 7432
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 9101
KasI G'GCGC_C
Cuts at: 5935 6531 6779 5935
Size: 596 248 8257
Fragments arranged by size:
8257 596 248
KpnI G_GTAC'C
Cuts at: 4998 6121 4998
Size: 1123 7978
MluI A'CGCG_T
Cuts at: 1511 8534 1511
Size: 7023 2078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 388 2297 3866 5042 7772 7956 388
Size: 1909 1569 1176 2730 184 1533
Fragments arranged by size:
2730 1909 1569 1533 1176 184
MscI TGG'CCA
Cuts at: 3532 3617 3688 5818 6862 3532
Size: 85 71 2130 1044 5771
Fragments arranged by size:
5771 2130 1044 85 71
MslI CAynn'nnrTG
Cuts at: 3682 3919 4070 4318 4484 4619 5363 7090 7217
Size: 237 151 248 166 135 744 1727 127
Cuts at: 7217 3682
Size: 5566
Fragments arranged by size:
5566 1727 744 248 237 166 151 135
127
MspA1I CmG'CkG
Cuts at: 159 727 745 763 784 1803 1842 2723 4344
Size: 568 18 18 21 1019 39 881 1621
Cuts at: 4344 5523 5697 6212 6526 6886 7899 8144 159
Size: 1179 174 515 314 360 1013 245 1116
Fragments arranged by size:
1621 1179 1116 1019 1013 881 568 515
360 314 245 174 39 21 18 18
NaeI GCC'GGC
Cuts at: 1105 1147 5561 5663 5812 7282 1105
Size: 42 4414 102 149 1470 2924
Fragments arranged by size:
4414 2924 1470 149 102 42
NarI GG'CG_CC
Cuts at: 5936 6532 6780 5936
Size: 596 248 8257
Fragments arranged by size:
8257 596 248
NcoI C'CATG_G
Cuts at: 5358 5573 5358
Size: 215 8886
NdeI CA'TA_TG
Cuts at: 2067 2067
Size: 9101
NgoMIV G'CCGG_C
Cuts at: 1103 1145 5559 5661 5810 7280 1103
Size: 42 4414 102 149 1470 2924
Fragments arranged by size:
4414 2924 1470 149 102 42
NspI r_CATG'y
Cuts at: 556 1601 2310 2988 3024 6154 6654 7561 556
Size: 1045 709 678 36 3130 500 907 2096
Fragments arranged by size:
3130 2096 1045 907 709 678 500 36
PasI CC'CwG_GG
Cuts at: 2352 2352
Size: 9101
PciI A'CATG_T
Cuts at: 2306 7557 2306
Size: 5251 3850
PflMI CCAn_nnn'nTGG
Cuts at: 2016 2723 4409 4912 2016
Size: 707 1686 503 6205
Fragments arranged by size:
6205 1686 707 503
PfoI T'CCnGG_A
Cuts at: 177 2915 5492 177
Size: 2738 2577 3786
Fragments arranged by size:
3786 2738 2577
PmeI GTTT'AAAC
Cuts at: 6100 6100
Size: 9101
PmlI CAC'GTG
Cuts at: 2081 2081
Size: 9101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 573 1726 1758 2488 2520 8240 8272 541
Size: 32 1153 32 730 32 5720 32 1370
Fragments arranged by size:
5720 1370 1153 730 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 265 1042 1170 2452 4422 4527 5863 5963 265
Size: 777 128 1282 1970 105 1336 100 3403
Fragments arranged by size:
3403 1970 1336 1282 777 128 105 100
PshAI GACnn'nnGTC
Cuts at: 1040 1040
Size: 9101
PspOMI G'GGCC_C
Cuts at: 2348 3473 5918 2348
Size: 1125 2445 5531
Fragments arranged by size:
5531 2445 1125
PspXI vC'TCGA_Gb
Cuts at: 1029 1029
Size: 9101
PstI C_TGCA'G
Cuts at: 1188 5961 6148 6833 1188
Size: 4773 187 685 3456
Fragments arranged by size:
4773 3456 685 187
PvuI CG_AT'CG
Cuts at: 104 3456 104
Size: 3352 5749
PvuII CAG'CTG
Cuts at: 159 727 763 784 1803 5523 5697 6526 6886
Size: 568 36 21 1019 3720 174 829 360
Cuts at: 6886 159
Size: 2374
Fragments arranged by size:
3720 2374 1019 829 568 360 174 36
21
RsrII CG'GwC_CG
Cuts at: 400 7296 400
Size: 6896 2205
SacI G_AGCT'C
Cuts at: 1499 2861 3041 6115 1499
Size: 1362 180 3074 4485
Fragments arranged by size:
4485 3074 1362 180
SalI G'TCGA_C
Cuts at: 5028 6138 5028
Size: 1110 7991
SanDI GG'GwC_CC
Cuts at: 4527 5863 4527
Size: 1336 7765
SapI GCTCTTCn'nnn_
Cuts at: 870 1897 2866 3662 3791 7124 7334 870
Size: 1027 969 796 129 3333 210 2637
Fragments arranged by size:
3333 2637 1027 969 796 210 129
SbfI CC_TGCA'GG
Cuts at: 5961 6148 5961
Size: 187 8914
ScaI AGT'ACT
Cuts at: 1468 1957 4183 8618 1468
Size: 489 2226 4435 1951
Fragments arranged by size:
4435 2226 1951 489
SexAI A'CCwGG_T
Cuts at: 3137 3713 3137
Size: 576 8525
SfcI C'TryA_G
Cuts at: 33 764 1184 1658 2209 5957 6144 6829 7822
Size: 731 420 474 551 3748 187 685 993
Cuts at: 7822 8013 33
Size: 191 1121
Fragments arranged by size:
3748 1121 993 731 685 551 474 420
191 187
SfoI GGC'GCC
Cuts at: 5937 6533 6781 5937
Size: 596 248 8257
Fragments arranged by size:
8257 596 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9101
SmaI CCC'GGG
Cuts at: 6123 6123
Size: 9101
SmlI C'TyrA_G
Cuts at: 1029 2758 2998 3169 3370 4807 5159 6256 7171
Size: 1729 240 171 201 1437 352 1097 915
Cuts at: 7171 7663 7925 1029
Size: 492 262 2205
Fragments arranged by size:
2205 1729 1437 1097 915 492 352 262
240 201 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9101
SphI G_CATG'C
Cuts at: 6154 6654 6154
Size: 500 8601
StuI AGG'CCT
Cuts at: 3112 3592 4456 3112
Size: 480 864 7757
Fragments arranged by size:
7757 864 480
StyI C'CwwG_G
Cuts at: 672 1391 1790 3740 4930 5358 5573 5636 6235
Size: 719 399 1950 1190 428 215 63 599
Cuts at: 6235 6589 672
Size: 354 3184
Fragments arranged by size:
3184 1950 1190 719 599 428 399 354
215 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 882 994 2535 6684 6817 7276 7485 232
Size: 650 112 1541 4149 133 459 209 1848
Fragments arranged by size:
4149 1848 1541 650 459 209 133 112
TatI w'GTAC_w
Cuts at: 1466 1955 4181 8616 1466
Size: 489 2226 4435 1951
Fragments arranged by size:
4435 2226 1951 489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1486 1518 2229 2261 2436 2468 3314 3346 8618
Size: 32 711 32 175 32 846 32 5272
Cuts at: 8618 8650 1486
Size: 32 1937
Fragments arranged by size:
5272 1937 846 711 175 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 404 3544 4544 6898 404
Size: 3140 1000 2354 2607
Fragments arranged by size:
3140 2607 2354 1000
XbaI T'CTAG_A
Cuts at: 4944 6132 4944
Size: 1188 7913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 185 1343 2529 4410 5643 6596 185
Size: 1158 1186 1881 1233 953 2690
Fragments arranged by size:
2690 1881 1233 1186 1158 953
XhoI C'TCGA_G
Cuts at: 1029 5159 1029
Size: 4130 4971
XmaI C'CCGG_G
Cuts at: 6121 6121
Size: 9101
XmnI GAAnn'nnTTC
Cuts at: 1268 2584 4351 1268
Size: 1316 1767 6018
Fragments arranged by size:
6018 1767 1316
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AlfI AscI AvrII BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I FspAI MfeI NheI NotI NruI
NsiI PacI PsiI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI